What are the chemical properties of 3- (N-Morpholinyl) -2-Hydroxypropanesulfonic Acid Sodium Salt?
3- (N-morpholinyl) -2-hydroxypropanesulfonate sodium salt is a commonly used biological buffer and is widely used in the field of biochemical research. It has the following chemical properties:
1. ** pH **: This compound is weakly acidic and will partially dissociate in aqueous solution, releasing protons, thus exhibiting acidic characteristics. Its sodium salt form is alkaline, because sodium ions can combine with hydroxide ions, making the solution alkaline. With this acid-base property, it can effectively regulate the pH of the solution, maintain stability within a specific pH range, and create a suitable environment for biochemical reactions.
2. ** Solubility **: This substance is highly soluble in water, which is one of its key advantages as a biological buffer. In aqueous solutions, it can be quickly and evenly dispersed to achieve efficient and precise adjustment of solution pH. And good solubility makes it compatible with various biomolecules and reagents, and will not affect the experimental process due to poor solubility.
3. ** Stability **: Under conventional conditions, 3- (N-morpholino) -2-hydroxypropane sulfonate sodium salt is quite stable and is not easily decomposed or deteriorated by factors such as light, heat, and air. However, although its stability is good, it should still be stored in a dry and cool place to avoid contact with strong oxidants, strong acids, strong bases and other substances to prevent chemical reactions from occurring and destroying its chemical structure, which in turn affects the buffering performance.
4. ** Buffering capacity **: As a buffer, it has excellent buffering capacity and can effectively resist large fluctuations in the pH value of the solution when a small amount of acid or base is added. This is due to the dissociable groups contained in its molecular structure, which can maintain the stability of the pH value of the solution through the release or acceptance of protons when the acidic or alkaline environment changes. The buffer range is usually between pH 6.5 and 7.9, which fits the appropriate pH range for chemical reactions in many organisms, providing a strong guarantee for the activity and stability of biomolecules.
What experiments is 3- (N-Morpholinyl) -2-Hydroxypropanesulfonic Acid Sodium Salt commonly used for?
3- (N-morpholino) -2-hydroxypropane sulfonate sodium salt, often used in biochemical experiments. It has the characteristics of good buffering performance and stable chemical properties, so it is widely used in many biochemical reaction systems.
It is often used in protein and nucleic acid related experiments. For example, when purifying proteins, it is necessary to create a suitable and stable environment. This salt can maintain a specific pH range, prevent proteins from denaturing due to pH changes, and ensure protein activity and structural integrity. In nucleic acid electrophoresis experiments, it is also indispensable to provide a stable buffer environment for nucleic acid migration, so that nucleic acids can swim orderly in gel media according to their own charge and size, achieving the purpose of separation and analysis.
also plays a key role in cell culture experiments. Cell growth is extremely sensitive to environmental pH. This salt can regulate the pH of the culture medium, meet the needs of cell growth, maintain normal cell physiological functions, and ensure the smooth progress of cell culture. When studying enzyme activity, different enzymes are best active under specific pH conditions. This salt can build and maintain the corresponding pH environment, helping researchers accurately explore enzyme kinetics and catalytic mechanisms.
3- (N-Morpholinyl) -2-Hydroxypropanesulfonic Acid Sodium Salt What should I pay attention to when storing?
3 - (N - morpholino) - 2 - hydroxypropane sulfonate sodium salt, this is a chemical reagent, when storing, many matters need to be paid attention to.
bear the brunt, temperature is crucial. It should be stored in a cool place, usually 2 - 8 ℃ is better. If the temperature is too high, it may cause a chemical reaction of the reagent, causing it to deteriorate and affecting the use effect. Such as in summer, if placed in a high temperature environment, the reagent may accelerate decomposition.
Humidity should not be ignored. It should be stored in a dry place, because the humidity is too high, it is easy for the reagent to absorb moisture. Once it absorbs moisture, or changes its chemical properties, such as agglomeration, it will not only be inconvenient to use, but also affect the accuracy of the experiment.
Furthermore, attention should be paid to avoiding light. This reagent may be sensitive to light, under light, or cause luminescent chemical reactions, causing its structure to change. Therefore, when storing, it should be placed in a brown bottle, or stored in a light-proof cabinet.
In addition, attention should also be paid to its isolation from other substances. Avoid co-storage with oxidizing, reducing substances, acids, alkalis, etc., to prevent chemical reactions. If it coexists with strong oxidants, it may cause violent reactions, or even safety risks.
Store in a well-ventilated place. If the ventilation is not good, the volatile gas of the reagent will accumulate, or it will pose a hazard to the surrounding environment and human health.
After taking it, it must be properly sealed. Prevent air from entering and avoid the reaction of the reagent with oxygen, carbon dioxide and other components in the air, so as to maintain its chemical stability. In this way, the properties of this reagent are kept stable during storage for subsequent experiments.
How soluble is 3- (N-Morpholinyl) -2-Hydroxypropanesulfonic Acid Sodium Salt?
3- (N - Morpholinyl) -2 - Hydroxypropanesulfonic Acid Sodium Salt, Chinese name 3- (N-morpholinyl) -2-hydroxypropane sulfonate sodium salt, often abbreviated as MOPSO-Na. The solubility of this substance is quite important, which is related to its performance in various experimental and industrial applications.
In terms of solubility, MOPSO-Na has good solubility in water. Because of its molecular structure, it contains both hydrophilic sulfonic acid groups (-SO-Na), which easily form ion-dipole interactions with water molecules, and nitrogen-containing morpholine rings and hydroxyl groups (-OH), which can form hydrogen bonds with water molecules. With these strong interactions, MOPSO-Na can be rapidly dispersed, ionized, and uniformly dissolved in water to form a clear and transparent solution.
In organic solvents, the solubility of MOPSO-Na is different. In polar organic solvents such as methanol and ethanol, because these solvents are similar to water, they can form hydrogen bonds or other interactions with MOPSO-Na, so there is a certain solubility. However, in non-polar organic solvents, such as benzene and n-hexane, MOPSO-Na is almost insoluble due to the mismatch of intermolecular forces.
In general, 3- (N - Morpholinyl) -2 - Hydroxypropanesulfonic Acid Sodium Salt has good solubility in water and some polar organic solvents, but poor solubility in non-polar organic solvents. This solubility characteristic makes it suitable for dissolution configuration in biochemistry and molecular biology experiments, as a buffer, etc., according to specific needs, to achieve the best application effect.
How does 3- (N-Morpholinyl) -2-Hydroxypropanesulfonic Acid Sodium Salt react with other reagents?
3- (N - Morpholinyl) -2 - Hydroxypropanesulfonic Acid Sodium Salt, Chinese name 3- (N-morpholinyl) -2-hydroxypropane sulfonate sodium salt, is often used as a biological buffer. The reaction between it and other reagents varies due to the wide variety of reagents it matches.
If it encounters a strong acid, because it is weakly basic, it will neutralize like a base and an acid to generate corresponding salts and water. For example, when reacting with hydrochloric acid, it will produce corresponding organic salts and water.
When in contact with metal ions, the hydroxyl groups and sulfonic acid groups in its structure have the ability to coordinate metal ions, or form complexes. Like binding to copper ions, complexes with specific structures and properties will be formed.
If it encounters a strong oxidant, some groups in the molecule, such as hydroxyl groups, may be oxidized, causing the molecular structure to change. For example, by strong oxidants such as potassium permanganate, hydroxyl groups may be oxidized to aldehyde groups, carboxyl groups, etc.
When coexisting with nucleophiles, the carbon atoms of the sulfonic acid group in the molecule may become a nucleophilic reaction check point, and the nucleophilic reagent attacks this check point and a nucleophilic substitution reaction occurs. When coexisting with some enzymes or proteins, it can adjust and maintain the pH stability of the system as a buffer, or affect the activity and structure of enzymes or proteins, ensuring that they function in a suitable pH environment.
