3- (N-Morpholinyl) -2-Hydroxypropylsulfonic Acid Mopso

3- (N - Morpholinyl) -2 - Hydroxypropylsulfonic Acid (Mopso) is a commonly used amphoteric ion buffer with the following chemical properties:
1. ** pH characteristics **: The pKa value of this substance is about 6.9 - 7.7. It can play a good buffering effect in the specific pH range of biochemical experiments (about pH 6.5 - 8.0). It can effectively maintain the stability of the pH value of the solution and resist the pH change caused by the addition of a small amount of acid or base. This property is essential in enzymatic reactions and protein studies that require precise control of the pH environment.
2. ** Solubility **: Easily soluble in water to form a clear and transparent solution. This good water solubility makes Mopso easy to disperse and apply in various aqueous experimental systems, which is conducive to its uniform mixing with other reagents during experimental operation and guarantees the accuracy and reproducibility of experimental results.
3. ** Chemical stability **: Under normal storage and use conditions, Mopso is quite stable in nature, not easy to volatilize, decompose or react with common chemicals. It can be stored for a long time and maintain its buffering properties unchanged, ensuring the reliability of experimental results and reducing errors caused by deterioration of reagents.
4. As a zwitterion, Mopso has a uniform charge distribution and little interference with the structure and activity of biological macromolecules such as proteins and nucleic acids. It can provide a stable pH environment in experiments to study the properties and functions of biomolecules without affecting the inherent properties of biomolecules, providing reliable conditions for experiments.

3- (N-morpholinyl) -2-hydroxypropanesulfonate sodium salt (Mopso) is widely used in biochemical experiments.
It is often used in protein and enzyme related experiments. In protein research, because proteins are sensitive to environmental pH, Mopso can maintain pH stability of the system, prevent protein denaturation, and maintain its structure and function integrity. For protein crystallization experiments, precise pH conditions are required. Mopso can regulate the environment and help proteins form regular crystals, so X-ray crystallography is used to analyze the structure.
In enzyme experiments, Mopso is also key. Enzyme activity is greatly affected by pH, and the activity is highest at a specific pH. Taking amylase as an example, when studying its hydrolyzed starch activity, Mopso is used with buffer solution to stabilize pH, which can accurately measure the change of enzyme activity under different conditions and explore the mechanism of enzyme action.
In cell culture experiments, Mopso is also used. Cell growth requires a suitable pH environment. Mopso participates in the buffer system of cell culture medium to maintain a stable pH, creating good conditions for cell growth and proliferation, and maintaining normal physiological functions and metabolic activities of cells.
In the field of nucleic acid research, such as DNA and RNA related experiments, Mopso can regulate the pH of the reaction system to ensure the stability of nucleic acid structure and prevent its degradation. It is important for nucleic acid extraction, amplification and sequencing to ensure the smooth progress of the experiment and the accuracy of the results.

3- (N-morpholinyl) -2-hydroxypropanesulfonic acid (Mopso) is a commonly used buffer in biochemical research. Its buffer range is crucial and affects the accuracy of many experiments. The buffer range of Mopso is about pH 6.5 - 7.9.
This buffer range is of great significance for specific biochemical reactions and experimental systems. The structure and function of many biomolecules are extremely sensitive to pH, and slight changes may cause changes in their activity. Mopso can effectively resist large fluctuations in pH caused by the addition of a small amount of acid or base in this pH range, so that the reaction system maintains a relatively stable acid-base environment.
In some enzymatic reactions, specific enzymes need to exhibit the best activity under a suitable pH environment. If the pH of the system deviates from the appropriate range, the enzyme activity may be inhibited or even inactivated. Mopso, with its buffering effect, can ensure the smooth progress of enzymatic reactions under stable pH conditions, ensuring the reliability and reproducibility of experimental results. Another example is protein electrophoresis experiments, a stable pH environment is also indispensable for protein separation and identification, and Mopso buffer can provide suitable acid-base conditions for such experiments.

Sodium 3- (N-morpholinyl) -2-hydroxypropanesulfonate (Mopso) is a commonly used amphoteric ion buffer for biochemical experiments. Its proper preservation is extremely important and is related to the accuracy of experimental results. According to the preservation of objects in "Tiangong Kaiwu", the following method should be followed:
The first environment is dry. Mopso is very easy to absorb moisture. If placed in a humid place, water vapor is easy to attach, causing deliquescence and affecting its purity and buffering performance. Therefore, a well-ventilated warehouse with low humidity should be selected, and a desiccant should be placed around, and it should be changed frequently to keep the environment dry.
The temperature is suitable. High temperature will make the chemical properties of Mopso lively, or cause decomposition; low temperature may make it crystallized, and it is difficult to dissolve when used. 15-25 ° C is the best. For example, the contents in "Tiangong Kaiwu" need to be timely, and the storage of Mopso needs to be suitable for temperature.
Furthermore, keep away from light. Light or promote photochemical reactions in Mopso, causing changes in composition. Use a brown bottle or opaque container to hold it in a place without direct light.
End and package sealed. Mopso is in too much contact with air and is easy to react with oxygen, carbon dioxide, etc. After taking it, seal it tightly in the original package as soon as possible, or reinforce it with a sealing film to prevent air from penetrating. In this way, Mopso must be properly preserved to make its performance stable and ready for experiments.

3- (N-morpholinyl) -2-hydroxypropanesulfonic acid (Mopso) is a commonly used buffer in biochemical research, and its stability under different pH conditions is very critical.
In an acidic environment, if the pH is low, the sulfonic acid group in the Mopso structure can remain stable. Because of its strong acidity and high degree of dissociation, it is not easily affected by protonation. However, the nitrogen atom of the hydroxyl group and the morpholinyl group may change the charge distribution and molecular configuration due to protonation. However, in the weakly acidic range of common buffering applications, such as pH 6-7, Mopso can maintain relatively stable, because the buffer system can resist large changes in pH and the structure will not change significantly.
Under neutral conditions, that is, when the pH is about 7, the sulfonic acid group of Mopso is completely dissociated and negatively charged, while the morpholine base is electrically neutral. In this case, the overall charge distribution of the molecule is relatively stable, and the interaction between the groups is also balanced. Therefore, Mopso has good stability and can effectively exert the buffering effect to ensure the constant pH of the solution.
In an alkaline environment, with the increase of pH, the hydroxyl group of Mopso may further dissociate, resulting in an increase in the negative charge of the molecule. If the alkalinity is too strong, the nitrogen atom of the morpholine group may be affected, or it may react with hydroxide ions, which will destroy the stability of the molecular structure. However, in its applicable alkaline range, such as pH 7-8.5, Mopso can still maintain structural stability by means of a buffering mechanism to ensure pH stability of the system.
In short, within the recommended pH range of 6.5-7.9 for Mopso, its stability is quite good, and it can effectively maintain the pH stability of the solution. It is suitable for a variety of biochemical experiments. However, beyond this range, its stability may be affected, the structure or properties may change, and the buffering efficiency may also be reduced.