3- [N, N-Di (Hydroxyethyl) Amino] What is -2-Hydroxypropanesulfonic Acid Dipso?
Sodium 3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonate (Dipso) is an important biochemical reagent. Its appearance is often white crystalline powder, which is widely used in the field of biochemical research.
This substance has excellent buffering properties and can effectively maintain the pH stability of a specific system. In many biochemical reactions and analytical experiments, precise control of pH is essential, and Dipso can achieve effective buffering within a specific pH range due to its unique chemical structure and properties. For example, in protein electrophoresis experiments, it is necessary to ensure that the pH value of the electrophoresis buffer is stable, so Dipso is often selected to provide a stable environment for protein separation and help the experimenter obtain accurate and reliable results.
Furthermore, Dipso has good solubility and can dissolve well in common solvents such as water. This property makes it easy to integrate into various experimental systems and easy to operate. Its chemical properties are relatively stable, and it is not easy to have unnecessary chemical reactions with other substances in the system, which can ensure the purity and stability of the experimental system.
In addition, in the fields of cell culture, Dipso is also used, which can create an acid-base environment suitable for cell growth and provide protection for normal physiological activities and metabolism of cells. In short, 3- [N, N-bis (hydroxyethyl) amino] -2-hydroxypropane sulfonate sodium (Dipso) plays a key role in many aspects of biochemical research due to its unique properties, promoting the continuous development and progress of related fields.
3- [N, N-Di (Hydroxyethyl) Amino] What are the main uses of -2-Hydroxypropanesulfonic Acid Dipso?
3- [N, N -bis (hydroxyethyl) amino] -2 -hydroxypropanesulfonate sodium (Dipso) is a commonly used buffer in biochemical research. Its main uses are roughly three.
One is that it plays a significant role in the buffer system of biochemical experiments. Biochemical reactions require a specific acid-base environment to proceed smoothly. Dipso can maintain the stability of the pH value of the system and will not be affected by minor changes in the pH in the reaction. For example, in enzymatic reactions, many enzymes have their own suitable pH range. Dipso can create a stable environment, so that the enzyme maintains good activity and ensures that the reaction occurs as expected, just like providing a stable "work room" for the enzyme.
Second, it is also indispensable in the study of proteins and nucleic acids. The structure and function of proteins and nucleic acids are extremely sensitive to the pH of the environment. Dipso can effectively stabilize the pH of the environment, protect the natural structure of proteins and nucleic acids, and enable their functions to play normally. If the pH of the environment fluctuates, proteins may denature, and the double-stranded structure of nucleic acids may be damaged, Dipso can protect its integrity, just like an umbrella company for proteins and nucleic acids.
Third, in the field of cell culture, it is also very useful. Cells grow in vitro, which needs to simulate the physiological environment in the body, and the stability of pH value is crucial. Dipso can adjust the pH of the medium, provide a suitable growth environment for cells, and help cells to proliferate and metabolize normally, as if to create a comfortable "home" for cells.
From this perspective, sodium 3- [N, N-bis (hydroxyethyl) amino] -2-hydroxypropanesulfonate (Dipso) plays a key role in many aspects of biochemical research, and is an indispensable and important reagent.
What are the chemical properties of 3- [N, N-Di (Hydroxyethyl) Amino] -2-Hydroxypropanesulfonic Acid Dipso?
3- [N, N -bis (hydroxyethyl) amino] -2 -hydroxypropanesulfonic acid (Dipso) is a very important biochemical reagent. Its chemical properties are unique and have the following numbers:
First, it is zwitterionic. The structure of this compound contains an amino group and a sulfonic acid group. In different pH environments, the amino group can obtain protons into cations, and the sulfonic acid group can release protons into anions. This makes it a buffer in a specific pH range and effectively maintains the stability of the pH of the solution.
Second, it has good solubility. It is easily soluble in water and can quickly and evenly disperse to form a stable and uniform solution, which is conducive to various biochemical experiments and provides a suitable liquid phase environment for related reactions.
Third, good chemical stability. Under common experimental conditions, it is not easy to react with other common chemicals, ensuring that the structure and properties of the experimental system are stable and do not interfere with the biochemical reaction studied, which can ensure the accuracy and reliability of the experimental results.
Fourth, the buffering ability is quite strong. In a specific pH range, it can efficiently neutralize and add a small amount of acid or base, so that the pH value of the solution fluctuates very little. For example, in biochemical analysis, many enzymatic reactions are extremely sensitive to pH. Dipso can create and maintain suitable pH conditions to ensure normal enzyme activity and help the reaction proceed smoothly.
3- [N, N-Di (Hydroxyethyl) Amino] -2-Hydroxypropanesulfonic Acid Dipso What are the precautions during use?
3- [N, N -bis (hydroxyethyl) amino] -2 -hydroxypropanesulfonate sodium (Dipso) is a commonly used buffer for biochemical experiments. When using, many precautions must be paid attention to.
Bear the brunt, the purity of Dipso is crucial. Impure Dipso contains impurities, or interfere with the experimental reaction, resulting in deviations in experimental results. If enzyme activity is measured, impurities or interactions with enzymes affect the accuracy of activity determination. Therefore, when choosing Dipso, high-purity products must be selected, and purity testing should be done before use.
Furthermore, the pH buffer range of Dipso cannot be ignored. The buffer range is about 6.9-8.3, and only within this range can the pH of the solution be effectively maintained. If it exceeds this range, the buffering capacity will drop sharply and the pH will fluctuate, which will affect the stability of the experimental system. For example, in cell culture experiments, pH fluctuations may cause cell growth to be inhibited or even death. Therefore, before the experiment, buffers must be precisely selected according to the needs of the experiment to ensure that the pH of the experimental system is in an appropriate range. The solubility of
Dipso also needs attention. Although it is easily soluble in water, factors such as temperature and solvent composition may affect the dissolution status. If the temperature is too low, the dissolution rate will be slow, and some will even be insoluble. When configuring the buffer, it is necessary to fully stir and heat it properly to promote its complete dissolution, and to ensure that the solvent is pure and no impurities
In addition, the compatibility of Dipso with other substances in the experimental system cannot be ignored. Certain metal ions, proteins, etc., may react with Dipso to change the buffering performance or affect the activity of other substances. For protein-related experiments, it is necessary to explore in advance whether Dipso interacts with proteins. If necessary, adjust the buffering agent or experimental conditions to prevent mutual interference.
At the end of the day, storage conditions are also critical. Dipso should be stored in a dry and cool place, protected from light and cool, to prevent it from absorbing moisture and deteriorating. If stored improperly, its properties will change, affecting the reproducibility and reliability of the experiment.
3- [N, N-Di (Hydroxyethyl) Amino] What is the approximate market price range for -2-Hydroxypropanesulfonic Acid Dipso?
3-% [N, N -bis (hydroxyethyl) amino] -2 -hydroxypropanesulfonate sodium (Dipso) is used in the biochemical reagent market, and its price range varies greatly due to quality, purity, packaging specifications, brands and purchase quantities.
If it is an ordinary laboratory grade, the purity is about 98%, and it is commonly packaged in 500 grams. The price of some ordinary domestic brands is roughly 200 to 500 yuan. This grade is suitable for general biochemical experiments and has a slightly higher tolerance for impurity content.
If it is a high purity, such as more than 99% purity, it is used in demanding scientific research projects. The same 500-gram package can cost 1,000 to 3,000 yuan for imported well-known brands. High-purity products have few impurities, which can ensure the accuracy and reproducibility of experiments, and are mostly used for high-end research.
Packaging specifications also affect the price. Small packages such as 25 grams, ordinary brands or 50 to 100 yuan are suitable for exploratory experiments with only a small amount of reagents. Large packages such as 1 kg or even more, although the unit price will be discounted due to bulk purchase, the total price is still higher, suitable for enterprises or institutions with large dosage.
In addition, the procurement channel is also related to the price. Buying directly from the manufacturer may be slightly lower due to the reduction of intermediate links. Buying through dealers, although convenient, may be slightly higher due to dealer price increases. The relationship between market supply and demand will also cause price fluctuations. During peak demand seasons or tight supply, prices may rise.