3-[Tris-(Hydroxymethyl)Methylamino]-2-Hydroxypropane Sulphonic Acid

3 - [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid, Chinese name 3 - [tris (methyl) methylamino] - 2 - propane sulfonic acid, often TAPSO. The main application domains of this compound are as follows:
First, in biochemical molecular biology, it plays an important role. Bio-biochemical polybiochemical reaction, TAPSO can be For example, in the analysis of some proteins, it is necessary to control the pH value of the protein to prevent the protein from being damaged. TAPSO can take advantage of its good performance to determine the pH environment, so that the protein can maintain its natural activity image, which is conducive to separation.
Second, it also has important uses in the field of artificial intelligence. For example, enzyme activity determination, immunoassay, etc., the pH value requirements of the reverse pH system are very strict. TAPSO can provide pH-determined components to ensure the reliability of the fruit. For example, in the serum of a specific enzyme activity, TAPSO can be established to determine the environment, which can avoid the deviation of enzyme activity caused by pH waves and help the baby to make sperm.
Third, it is also indispensable in cell culture. The pH value of the cell environment is demanding, and TAPSO can reduce the pH value of the cell culture and provide a good environment for the cell. For example, in some sensitive cell cultures, TAPSO can maintain the pH of the cell culture, promote cell wall growth and proliferation, prevent cell failure or death due to pH failure, and ensure cell growth.

3 - [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid, Chinese name is tris (methyl) methylamino - 2 - propane sulfonic acid, TAPSO. This compound is a commonly used biological product, and it is very important to characterize it at different pH environments.
Generally speaking, TAPSO can exhibit excellent performance at a pH close to its pKa value, which makes it well-characterized. The pKa value of TAPSO is between 7.6 and 9.0. At this pH, it can effectively maintain the pH value of the solution and resist the addition of a small amount of acid or water. It is due to the fact that the functional properties of the TAPSO molecule are high, and the TAPSO molecule is inclined to accept H to form a phase. If the pH value is low and exceeds the pH value, the TAPSO's performance will decrease, and the quality will also be affected, or the biochemical reaction will occur, causing it to change.
Conversely, if the pH value is high and the pKa value is high, the OH degree in the solution will be large, and some functions in the TAPSO molecule may release H to neutralize OH. However, if the pH value is high, beyond the, the identity of TAPSO will decrease, and may even decompose or reverse other substances.
, 3- [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid has good qualitative ability at pH levels close to its pKa value, and at this pH, its qualitative may be affected to varying degrees.

3 - [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid is 3- [Tris (Hydroxymethyl) Methylamino] -2 - Hydroxypropane Sulfonic Acid, often referred to as TAPSO, is a biological buffer. The preparation method is as follows:
Starting material, an appropriate amount of trimethylamine, epoxy chloropropane and 1,3 - propane sulfonate lactone should be prepared. In the first step, trimethylamine aqueous solution is mixed with epichlorohydrin according to a certain molar ratio. At a moderate temperature, such as 30-50 ° C, the reaction is stirred for several hours. During this process, the nitrogen atom of trimethylamine nucleophilically attacks the epoxy ring of epichlorohydrin, and the ring is opened to form an intermediate containing hydroxyl groups and chlorine atoms. After the reaction is completed, the excess trimethylamine and water are removed by distillation under reduced pressure to obtain the intermediate product.
In the next step, the obtained intermediate and 1,3-propane sulfonate lactone are put into the reactor in an appropriate proportion, and an appropriate amount of organic solvent, such as acetone or acetonitrile, is added to promote the reaction to proceed uniformly. Heat up to 50-70 ° C At this time, the sulfonic acid negative ion of 1,3-propane sulfonate lactone undergoes nucleophilic substitution reaction with the chlorine atom of the intermediate to form the target product TAPSO.
The reaction ends, the temperature is lowered, and insoluble impurities are removed by filtration. The filtrate is concentrated under reduced pressure to recover the organic solvent. The residue is recrystallized with an appropriate amount of ethanol or isopropanol to precipitate white crystalline TAPSO. The crystals are filtered and collected, and dried in vacuum to obtain a high-purity 3-[ tris (hydroxymethyl) methylamino] -2-hydroxypropane sulfonic acid product. Throughout the preparation process, attention should be paid to the precise control of the reaction temperature, material ratio and reaction time to ensure product quality and yield.

3 - [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid, Chinese name is often 3- (N-tris (hydroxymethyl) methylamino) -2-hydroxypropane sulfonic acid, referred to as TAPSO, is a commonly used zwitterion buffer in biochemical experiments. Its compatibility with other chemical substances is crucial to the success or failure of experiments and the accuracy of results, which should not be underestimated.
In the biochemical experimental environment, TAPSO coexists harmoniously with many common reagents, such as salts and sugars, without conflict. Salts in solution can adjust the ionic strength, while the chemical structure of TAPSO is stable and the charge distribution is balanced, so it is mixed with common salts such as sodium chloride and potassium chloride, and the buffering performance is not damaged, and the solution system is also safe. Sugars such as glucose and sucrose are mostly protective agents for biomolecules. When coexisting with TAPSO, there is no chemical reaction between the two, and their respective functions can be played normally.
Proteins and enzymes are key components of biological systems. The buffer range of TAPSO is suitable and close to neutral, which can simulate the physiological environment in organisms. In the research experiments of proteins and enzymes, it can effectively maintain its activity and structural integrity. Due to its mild chemical properties, it does not interact with the activity check points of proteins and enzymes, so it can be used as an excellent buffer medium for the study of such biomacromolecules.
However, TAPSO is not compatible with all substances. Strong oxidants and strong reducing agents can react with TAPSO due to their active chemical properties, resulting in structural damage and loss of buffer function. Solutions containing heavy metal ions, such as mercury ions, lead ion solutions, or complexing with some groups of TAPSO, change their chemical properties and buffer properties.
Therefore, at the beginning of the experimental design, the compatibility of TAPSO with other chemical substances used must be checked in detail. If there is any doubt, we should first explore the interaction with small-scale experiments to ensure that the experimental system is stable and the results are accurate, which is the key to the success of the experiment.

3 - [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid, which is also the same. It is commonly used in polybiochemical processes and work processes, and is determined by the pH of the system.
As for the impact of its environment, it needs to be increased. Its chemical properties are determined, and it can be rapidly degraded in the natural environment, or it can be tired. If a large amount of water is added to the water, or the pH value of the water is changed, it will affect the survival of aquatic organisms. Water-sensitive organisms are sensitive to pH degradation, which affects their physiological generation, causing their death and breaking the balance of aquatic life.
and other substances in it or in the water react biochemically, forming unknown by-chemicals, and the environmental effects of these by-chemicals are also unknown. If it enters the soil, it may affect the acidity of the soil and the microbial community, which can affect the growth and cultivation of plants.
However, under proper use and proper management, it can reduce the harm of its environment. For example, control its usage and manage it before discharge to reduce its environmental dosage. In addition, 3 - [Tris - (Hydroxymethyl) Methylamino] - 2 - Hydroxypropane Sulphonic Acid. There is a certain shadow in the environment, so be careful.