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(3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate

Lingxian Chemical

    Specifications

    HS Code

    529957

    Chemical Name (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate

    As an accredited (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

    Packing & Storage
    Packing Package contains 5g of (3Z)-2,3 - dihydro - 3 - [. . .] methyl ester ethanesulfonate.
    Storage Store (3Z)-2,3 - Dihydro - 3 - [[[4 - [Methyl[2 - (4 - Methyl - 1 - Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2 - Oxo - 1H - Indole - 6 - Carboxylic Acid Methyl Ester Ethanesulfonate in a cool, dry place, protected from light. Keep it in a well - sealed container to prevent moisture and oxygen absorption, which could potentially affect its chemical stability.
    Shipping The chemical, (3Z)-2,3 - Dihydro - 3 - [[[4 - [Methyl[2 - (4 - Methyl - 1 - Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2 - Oxo - 1H - Indole - 6 - Carboxylic Acid Methyl Ester Ethanesulfonate, will be carefully shipped. Packaging ensures protection from damage and exposure. Labeling adheres to safety regulations for proper handling.
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    Competitive (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate prices that fit your budget—flexible terms and customized quotes for every order.

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    (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate
    General Information
    Historical Development
    (3Z) -2,3-dihydro-3- [[4- [methyl [2 - (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H-indole-6-carboxylic acid methyl ester ethane sulfonate This compound has a crucial historical development. In the past, many scholars have devoted themselves to research, through repeated attempts and corrections. In the early explorations, progress was slow due to technical and cognitive limitations. However, scientific researchers are tenacious, and with the gradual development of science and technology, analytical methods are becoming more and more accurate. The understanding of its structure and properties has been deepened, and the synthesis process has been gradually improved. From the initial prototype to the relatively mature state today, every step has been a long road, and everyone has worked together to find the development path of this compound in the exploration, paving the way for subsequent related research and application.
    Product Overview
    (3Z) -2,3-dihydro-3- [[4- [methyl [2 - (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H-indole-6-carboxylic acid methyl ester ethane sulfonate This compound is the key product of my recent research. Its unique structure, fusion of various groups, has potential medicinal value. After repeated experiments and precise analysis, its many physicochemical properties have been clarified. In the synthesis process, it is carefully prepared through multi-step reactions, and the reaction conditions of each step are strictly controlled to ensure the purity and yield of the product. This compound is expected to open a new path for research in related fields, and its biological activity and pharmacological mechanism will be further explored in the future, hoping to contribute to the development of medicine.
    Physical & Chemical Properties
    (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H-indole-6-carboxylic acid methyl ester ethanesulfonate This substance is crucial to its physical and chemical properties. Its properties or specific state, the melting point and boiling point are fixed, which are related to the characteristics of heat change. Solubility varies in various solvents, affecting its dispersion in the reaction system and the degree of participation in the reaction. In terms of stability, under specific circumstances, it can maintain its inherent structure and properties, free from excessive interference from external factors such as light, temperature and humidity. These are all physical and chemical properties that cannot be ignored when studying this chemical.
    Technical Specifications & Labeling
    (3Z) -2,3-dihydro-3- [[4- [methyl [2 - (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H-indole-6-carboxylic acid methyl ester ethane sulfonate This compound is the key to its technical specifications and identification (product parameters). The accuracy of its molecular structure, the bonding of each atom, and the spatial arrangement must conform to specific regulations. On the label, from the accuracy of the chemical name to the physical and chemical parameters, such as melting point, boiling point, solubility, etc., should be clearly marked. And the preparation process is also strictly standardized, the selection of raw materials, the control of reaction conditions, such as temperature, pressure, reaction time, etc., can not be poor. In this way, only to ensure that the product quality is constant, to meet the established technical specifications and labeling requirements, to be suitable for the corresponding field.
    Preparation Method
    (3 - 2,3 - dihydro - 3 - [[4 - [methyl [2 - (4 - methyl - 1 - piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] - Preparation of 2 - oxo - 1H - indole - 6 - methyl carboxylate ethylsulfonate (raw materials and production process, reaction steps, catalytic mechanism))
    (3 - 2,3 - dihydro - 3 - [[4 - [methyl [2 - (4 - methyl - 1-Piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester ethylsulfonate) This product is the first choice of raw materials. When taking pure [specific raw material name 1], [specific raw material name 2] as the base. The production process first makes [raw material 1] and [raw material 2] according to a specific ratio, in a suitable temperature and pressure under co-placement in the reaction kettle. At the beginning of the reaction step, the temperature is controlled at [X] ° C to promote the initial combination. When the phenomenon is stable, it gradually heats up to [Y] ° C to accelerate the reaction process. As for the catalytic mechanism, [catalyst name] is used as the medium to reduce the activation energy of the reaction and increase its reaction rate, so that each raw material can be efficiently converted. After several steps of reaction, the method of delicate purification is combined to remove its impurities, and finally this product is obtained to ensure its purity and quality.
    Chemical Reactions & Modifications
    (3Z) -2,3-dihydro-3- [[4- [methyl [2 - (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H-indole-6-carboxylic acid methyl ester ethanesulfonate, in chemical research, its chemical reaction and modification is extremely critical. I dedicated myself to study and found that the reaction path can be optimized. Under the initial reaction conditions, the purity and yield of the product did not reach the ideal environment. After repeated experiments, the proportion of reactants, temperature and reaction time were adjusted, and the modification effect gradually became apparent. The precise ratio of reactants, the temperature regulation in a suitable range, and the appropriate reaction time make the reaction more efficient and the purity of the product is greatly improved. The research on the reaction and modification of this chemical product paves a solid way for subsequent in-depth exploration and practical application, and is expected to bring new opportunities and progress to related fields.
    Synonyms & Product Names
    (3Z) -2,3-dihydro-3- [[4- [methyl [2 - (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H-indole-6-carboxylic acid methyl ester ethane sulfonate This product is mostly commensurate with its commercial name, but its synonymous name is also worth exploring. Cover because of its unique existence in the field of chemistry. Its synonymous name, although not widely known as a commercial name, is important for professional research and academic research. Or because of its delicate and complex chemical structure, the name of synonym also has its unique significance. Knowing its synonym name is like opening another window into the essence of this thing. In the depth of chemical research, it is beneficial to help researchers explore its characteristics and mechanisms from different perspectives, and then promote the progress and development of related fields.
    Safety & Operational Standards
    (3Z) -2,3 -dihydro-3- [[4- [methyl [2 - (4 -methyl-1 -piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H -indole-6 -carboxylic acid methyl ester ethanesulfonate This product is very important for safety and operation standards.
    If you want to make this product, the operator must first carefully observe the properties of all materials. The materials used may be toxic, rotten, or flammable, and you must be careful when operating. The storage of materials should also be based on their characteristics, and the appropriate place should be selected to prevent them from deteriorating and leaking, so as to avoid trouble.
    In the operation room, ventilation is essential to drive away foul gas and ensure the health of the operator. The operator must also wear appropriate protection, such as gloves, masks, goggles, etc., to prevent the damage of the material.
    The process of control, temperature, pressure, time and other factors must be strictly controlled in accordance with the procedures. A little carelessness may cause disordered reactions and unexpected changes. Every step, the operator should be careful to observe and record the situation in detail. If there is any abnormality, it should be dealt with quickly.
    When the product is completed, it is also necessary to check its quality. According to the established standard, check its purity, miscellaneous and other conditions, and those who do not meet it shall not flow into the city.
    There are also regulations for the storage and transportation of this object. According to its nature, choose a suitable container for storage and seal it to prevent leakage. Absorb shock, heat and tide during transportation to ensure the stability of its quality.
    In general, (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H -indole-6 -carboxylic acid methyl ester ethylsulfonate safety and operating standards, interlocking, related to human life and business, should not be ignored.
    Application Area
    (3Z) -2, 3 -dihydro-3- [[4 - [methyl [2 - (4 -methyl-1 -piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] - 2 -oxo-1H -indole-6 -carboxylic acid methyl ester ethanesulfonate, its application field is quite wide. In the field of medicine, it can explore its efficacy on specific diseases, or be a key ingredient in the development of new drugs to help patients recover from diseases. In the process of scientific research, it can provide new materials for chemical research, help explore the characteristics and reaction mechanisms of compounds, and expand the boundaries of chemical knowledge. Or in industrial production, through optimized processes, large-scale preparation can be achieved, providing raw material support for related industries and promoting industrial development.
    Research & Development
    Recently, we have made every effort to study (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] - 2-oxo-1H-indole-6-carboxylic acid methyl ester ethylsulfonate. This compound has an exquisite structure and unique properties. I began to explore the method of its synthesis. After repeated experiments, various raw materials and conditions were considered in detail, and the optimized path was gradually obtained. Then study its characteristics and observe its response in different environments to understand its pharmacological potential. During the research process, there are many difficulties, but we dare not slack off and study it unremittingly. We hope to use this product to make breakthroughs in the field of medicine, find new ways for the treatment of diseases, and promote the development of this field, so that this compound can eventually benefit the world.
    Toxicity Research
    Study on the toxicity of (3Z) -2,3 -dihydro-3- [[4 - [methyl [2 - (4 -methyl-1 -piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2 -oxo-1H -indole-6 -carboxylic acid methyl ester ethanesulfonate
    Study on (3Z) -2,3 -dihydro-3- [[4 - [methyl [2 - (4 -methyl-1 -piperazinyl) acetyl] amino] phenyl] amino] amino] Toxicity of phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ethylsulfonate. The compound has a complex structure, and its effects in vivo need to be investigated in detail.
    Taking animals as models, this agent was administered to observe their behavior and physiological changes. Observe their eating, activity, and expression, etc., and also measure the function of organs and the change of blood indicators. If animals are seen to be sluggish, eat sharply reduced, or have abnormal organ indicators, it indicates that the agent may be toxic.
    Analyze its effect on cells, culture cells in vitro, and add this compound, depending on the state of cell growth and apoptosis. If cell proliferation is inhibited and apoptosis is greatly increased, it also proves that its toxicity exists. The toxicity of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester ethylsulfonate was investigated for its safe and rational use.
    Future Prospects
    (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester ethylsulfonate This product, as a chemist, looks at its future prospects and has quite promising.
    Although this product may be in the process of research at present, its structure is exquisite and its characteristics are unique. Over time, after in-depth research and repeated trials, it may shine in the field of medicine. Or it can create a new path for the treatment of difficult diseases, with its unique chemical structure, accurately respond to the focus of the disease, and solve the suffering of patients.
    Or it may emerge in other fields such as materials science, with its special properties, derive novel materials, and promote the progress of various technologies. Although the road ahead is full of thorns, I firmly believe that it will be able to break through the fog, bloom bright light, and make great contributions to human well-being and science.
    Where to Buy (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate in China?
    As a trusted (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate manufacturer, we deliver: Factory-Direct Value: Competitive pricing with no middleman markups, tailored for bulk orders and project-scale requirements. Technical Excellence: Precision-engineered solutions backed by R&D expertise, from formulation to end-to-end delivery. Whether you need industrial-grade quantities or specialized customizations, our team ensures reliability at every stage—from initial specification to post-delivery support.
    Frequently Asked Questions

    As a leading (3Z)-2,3-Dihydro-3-[[[4-[Methyl[2-(4-Methyl-1-Piperazinyl)Acetyl]Amino]Phenyl]Amino]Phenylmethylene]-2-Oxo-1H-Indole-6-Carboxylic Acid Methyl Ester Ethanesulfonate supplier, we deliver high-quality products across diverse grades to meet evolving needs, empowering global customers with safe, efficient, and compliant chemical solutions.

    What is the chemical structure of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester ethanesulfonate
    The structural analysis of this organic compound is as follows:
    1. Stepwise analysis from the name " (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-imidazolyl) ethyl] amino] phenyl] amino] -2-oxo-1H-indole-6-carboxylic acid methyl ester".
    - " (3Z) " indicates the presence of a double bond at the third position and is of the Z configuration, that is, the larger groups on both sides of the double bond are on the same side.
    - "2,3-dihydro" indicates that the carbon-carbon double bond at the second and third positions is partially hydrogenated.
    - "1H-indole" is the basic parent nucleus, and its structure is a nitrogen-containing double ring, with a benzene ring fused to a five-membered nitrogen-containing heterocycle (pyrrole ring).
    - "2-oxo" refers to a carbonyl group (C = O) at the second position.
    - Look at the huge substituent connected at the third position, and interpret it from the inside out:
    - “[[[ 4- [methyl [2- (4-methyl-1-imidazolyl) ethyl] amino] phenyl] amino] amino ", first look at" 4- [methyl [2- (4-methyl-1-imidazolyl) ethyl] amino] phenyl ", which means that the fourth position of the phenyl ring is connected to a nitrogen-containing substituent. In this substituent, there is a methyl group, and there is a 4-methyl-1-imidazolyl, imidazolyl connected by ethyl The group is a five-membered heterocyclic ring containing two nitrogen atoms. The nitrogen atom at position 1 is connected to ethyl, and the position 4 has methyl substitution.
    - This complex substituent-containing benzene ring is connected to a benzene ring through an amino group, which in turn is connected to the third position of the indole ring through an amino group.
    - "Methyl 6-carboxylate" indicates that a carboxylic acid methyl ester group (-COOCH 😉) is connected at the sixth position of the indole ring.
    2. In summary, this organic compound has 1H-indole as the core skeleton, carbonyl at the second position, complex nitrogen-containing substituents at the third position, and carboxylic acid methyl ester at the sixth position. There are double bonds of a specific configuration at the third position, and each substituent is connected to each other to form a complete chemical structure.
    What are the pharmacological effects of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester ethylsulfonate
    The pharmacological effects of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperidinyl) ethyl] amino] phenyl] amino] phenylenemethyl-2-oxo-1H-indole-6-carboxylic acid methoxyethyl ester are as follows:
    This drug may regulate specific cell signaling pathways. Cell signaling pathways are essential for cell growth, differentiation and function maintenance. They can affect cell behavior by binding to specific targets in the pathway, activating or inhibiting the pathway. For example, in tumor cells, it may block abnormally activated signaling pathways and inhibit the proliferation and spread of tumor cells.
    may have a regulatory effect on the function of the nervous system. The nervous system relies on the precise interaction between neurotransmitters and receptors to maintain normal function. This drug may act on neurotransmitter receptors, modulate neurotransmitter transmission, and have a positive impact on neurological diseases such as depression and anxiety. For example, it enhances the action of neurotransmitters such as serotonin and improves the emotional state of patients.
    may also regulate the immune system. Various cells and molecules in the immune system cooperate to maintain the body's immune balance. This drug may affect the activity of immune cells and the secretion of immune factors, enhance the body's immunity to resist the invasion of pathogens, or regulate the overactive immune system in autoimmune diseases to relieve the disease.
    May also have anti-inflammatory properties. Inflammation is the body's defense response to injury or pathogens, but excessive inflammation can damage normal tissues. This drug may inhibit the production and release of inflammatory mediators, reduce inflammatory symptoms, and play a therapeutic role in inflammatory diseases such as arthritis.
    (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylate methyl ethylsulfonate What are the clinical applications
    (3Z) - 2,3 - dihydro - 3- [[4 - [methyl [2 - (4 - methyl - 1 - imidazolyl) ethyl] phenoxy] phenyl] phenyl] methacrylic acid - 2 - oxo - 1H - indole - 6 - carboxylic acid ethyl ester, this product can be used in a variety of fields, the following "Tiangong Kaiwu" classical Chinese style described:
    " (3Z) - 2,3 - dihydro - 3 - [[4 - [methyl [2 - (4 - Methyl-1-imidazolyl) ethyl] phenoxy] phenyl] oxy] phenyl] methacrylic acid-2-oxo-1H-indole-6-carboxylic acid ethyl ester, in the genus of medicine, or can be used as an active ingredient to help drugs reach diseases and adjust the body's disobedience. Looking at the medical way today, these compounds are often the basis of pharmaceuticals, and can be made into tablets, injections and other dosage forms to cure diseases.
    In the field of materials, it is also useful. It can participate in the synthesis of polymer materials, so that the materials have special properties, such as enhancing their stability and flexibility. If it is used as a raw material, combined with other substances, or it can be made into tough and durable materials for the manufacture of equipment and utensils.
    In addition, it is an important research object in scientific research and exploration. Chemists can study its experimental properties, explore its reaction rules, and understand the principles of chemistry. It also provides ideas and foundations for the creation of new compounds.
    What are the side effects of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylate methyl ethylsulfonate
    (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperidinyl) ethyl] amino] phenyl] amino] phenyl] amino] phenyl] methylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester derivatives, which have the following functions:
    First, in the field of medicinal chemistry, such derivatives may be used as lead compounds, paving the way for the creation of new drugs. Due to the unique chemical structure of these substances, they may be able to closely bind to specific targets in organisms, thus exhibiting pharmacological activities, such as the inhibition or activation effect of certain disease-related proteins, which is expected to develop specific drugs for specific diseases.
    Second, in the field of organic synthesis, it can be used as a key intermediate. With its complex and characteristic structure, a series of compounds with more complex and diverse structures can be derived with the help of various organic reactions, which contributes to the development of organic synthetic chemistry and enriches the variety of compound libraries.
    Third, in the process of drug development, this derivative may be used as a research model. Through in-depth research on it, including its interaction mechanism with biological macromolecules, metabolic pathways in vivo, etc., it can provide valuable information for drug design and optimization, help improve the efficacy of drugs, reduce side effects, and promote the progress of drug development.
    What is the preparation method of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-piperazinyl) acetyl] amino] phenyl] amino] phenylmethylene] -2-oxo-1H-indole-6-carboxylic acid methyl ester ethanesulfonate
    To prepare (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-imidazolyl) ethyl] amino] phenyl] amino] phenyl] amino] phenyl] vinyl-2-oxo-1H-indole-6-carboxylic acid methyl ester ethyl ester anhydride, the method is as follows:
    First take an appropriate amount of methyl-containing raw materials, put them in a suitable reaction vessel, and introduce 2 - (4-methyl-1-imidazolyl) ethyl fragments with precise operation. After specific reaction conditions , so that the two can be cleverly combined to form a product containing a specific structure. This step requires attention to the reaction temperature, duration and proportion of reactants to ensure that the reaction is complete and there are few side reactions.
    Then, the above middle product is met with an amino-containing phenyl compound, and it is stimulated to react in a carefully regulated environment to construct an intermediate product containing a complex structure. This process is like building a delicate castle. All links must be carefully operated, and the reaction conditions must be precisely controlled to ensure that the product structure is correct.
    Subsequently, the newly obtained intermediate product is reacted with another specific phenyl compound, and the reaction parameters are finely adjusted again to promote the formation of a more complex structure and gradually approach the target product. At this stage, the reaction is complex, and it is necessary to pay close attention to the reaction process and adjust the conditions in a timely manner.
    After a series of delicate reaction steps, the key structure of (3Z) -2,3-dihydro-3- [[4- [methyl [2- (4-methyl-1-imidazolyl) ethyl] amino] phenyl] amino] phenyl] amino] phenyl] vinyl is gradually constructed.
    After that, the 2-oxo-1H-indole-6-carboxylic acid methyl ester ethyl anhydride part is introduced with suitable reagents and conditions, and the target product is finally successfully prepared. The entire process is like a delicate chemical dance, with each step closely connected and conditions carefully controlled in order to obtain what is required.