4-(2-Hydroxyerhyl)Piperazine-1-Erhanesulfonic Acid

Lingxian Chemical

Specifications

HS Code	739346
Chemical Formula	C8H18N2O4S
Molar Mass	238.305 g/mol
Appearance	White to off - white powder
Solubility	Soluble in water
Pka Value	~7.5
Ph Range For Buffering	6.8 - 8.2
Stability	Stable under normal conditions
Melting Point	~235 - 239 °C
Density	N/A (as a solid powder)
Hygroscopicity	May be hygroscopic

Packing & Storage

Packing	500g of 4-(2 - Hydroxyethyl)Piperazine - 1 - ethanesulfonic Acid in a sealed plastic bag.
Storage	4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) should be stored in a cool, dry place, away from direct sunlight. Keep it in a tightly sealed container to prevent moisture absorption. Since it may react with strong oxidizing agents, store it separately from such substances. Ideal storage conditions help maintain its chemical stability for reliable use in various biochemical applications.
Shipping	Ship 4-(2 - Hydroxyethyl)Piperazine - 1 - Ethanesulfonic Acid in well - sealed containers suitable for chemicals. Ensure proper labeling for hazard and handling. Ship via methods compliant with chemical transportation regulations, maintaining appropriate storage conditions during transit.

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4-(2-Hydroxyerhyl)Piperazine-1-Erhanesulfonic Acid

General Information

Historical Development

The development of 4- (2 - Hydroxyerhyl) Piperazine - 1 - Erhanesulfonic Acid (4- (2 - Hydroxyerhyl) Piperazine - 1 - Erhanesulfonic Acid) can be traced back to the past. In the past, the academic community studied biochemical reagents and strived to precisely regulate the reaction environment. When this agent first appeared, it was only used by niche scientific research. However, with the passage of time and the expansion of scientific research, there is a growing demand for stable buffer systems in many fields.
Its use in biomedical experiments and cell culture has gradually become critical. It can maintain an appropriate pH and ensure the safe growth of cells. And in protein research and other aspects, it escorts experimental stability. From being little known at first to being widely used today, 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid has undergone changes and become an indispensable aid in the scientific research process, promoting biochemical research to a new direction.

Product Overview

4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, this is a delicate compound. Its mild nature and unique chemical characteristics. Looking at its structure, piperazine is cyclized with hydroxyethyl and ethanesulfonic acid groups, complementing each other and forming a delicate state.
In the field of biochemical research, it has a wide range of uses. It is often used as a buffer, which can stabilize the pH value of the solution and provide a suitable acid-base environment for many biochemical reactions. It is like a quiet harbor, protecting the smooth progress of the reaction. Its buffering capacity is quite good, and it can cope with minor acid-base changes and ensure the stability of the system.
The preparation method, through multiple fine processes, with rigorous processes and precise control, obtains this pure product. Only with high quality and few impurities can they fully demonstrate their capabilities in scientific research experiments, assist in biochemical exploration, and contribute to the progress of scientific research.

Physical & Chemical Properties

4- (2-Hydroxyerhyl) Piperazine-1-ethanesulfonic acid (4- (2-Hydroxyerhyl) Piperazine-1-Erhanesulfonic Acid), its physicochemical properties are crucial. This substance is in the form of a white crystalline powder, which is easily soluble in water. Its melting point is in a specific range and exhibits good thermal stability. In terms of solubility, it has good solubility in polar solvents, but limited solubility in non-polar solvents. In terms of pH, it has a certain buffering capacity and can maintain the pH stability of the solution in a specific pH range. These physicochemical properties make it widely used in many fields, such as as as a buffer in biochemical experiments, which can effectively ensure the stability of the reaction environment and help the smooth progress of various experiments.

Technical Specifications & Labeling

Technical Specifications and Labeling of 4- (2-Hydroxyethyl) Piperazine-1-ethanesulfonic Acid (Product Parameters)
For those with 4- (2-Hydroxyethyl) Piperazine-1-ethanesulfonic Acid, the technical specifications are the key. The selection of raw materials must be pure and free of impurities, and the preparation process should be accurate. The reaction temperature should be stable in an appropriate range, and the fluctuation should not exceed the specified value to prevent the product from being impure.
As for the label, the chemical name of the product should be 4- (2-Hydroxyerhyl) Piperazine-1 - Erhanesulfonic Acid, and the important product parameters should be listed in detail. Such as purity geometry, impurity content, should be clearly marked, so that users can see at a glance, in order to comply with technical specifications and identification of the right path.

Preparation Method

The method for making 4 - (2 - hydroxyethyl) piperazine - 1 - ethanesulfonic acid is as follows: Take an appropriate amount of raw materials, among which pure ones need to be selected to ensure quality. First, in the clean vessel, add the corresponding materials according to a certain ratio. This material ratio is related to the quality of the finished product, and must be accurate.
Mix the materials and stir them, control their temperature and speed, and meet the needs of the reaction. When reacting, observe its changes and proceed in sequence. First, make the materials fully blend, then heat up, initiate a reaction, and control the temperature according to time to prevent excessive or insufficient.
After the reaction is completed, the step of purification is carried out. In a specific way, the impurities are removed and the essence is retained. The purified matter is then finely processed to obtain the required finished product. The whole process is closely interlocked at each step, which is related to success or failure. All steps should be done with caution to make high-quality products.

Chemical Reactions & Modifications

Today there is a substance, named 4- (2 - Hydroxyerhyl) Piperazine - 1 - Erhanesulfonic Acid. I am a chemical researcher, studying the chemical reaction and modification of this substance.
View its structure and think about the path of its reaction. Try it under various conditions to determine its characteristics. Or adjust the temperature, or change the amount of agent, observe its change.
After several tests, it is known that it is in a certain environment and can play a specific role. And other methods can be used to change its properties to suit various needs. If additives are added to make the reaction smooth, it can also change its shape and quality and increase its use.
This research in the chemical industry may be of great benefit. Looking forward to the future, I will use the knowledge I have gained to promote the wider use of this thing to benefit everyone.

Synonyms & Product Names

4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, which is widely used in biochemical research. There are many other names, such as HEPPSO, which is also a common trade name. In the context of biochemical experiments, it is often called HEPPSO to facilitate communication and recording.
Looking at its name, 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, the expression is accurate, but slightly complicated. HEPPSO is concise and easy to remember, and it is often heard in the mouth of everyone in the laboratory. Although the two have different names, they actually refer to the same thing, just like the ancient aliases and correct names, both of which are signs of recognizing and using this thing to help the road of biochemical research.

Safety & Operational Standards

4 - (2 - Hydroxyerhyl) Piperazine - 1 - Erhanesulfonic Acid (4 - (2 - Hydroxyerhyl) Piperazine - 1 - Erhanesulfonic Acid) is the chemical we have been working on. It is a matter of safety and operating standards. It is extremely important and cannot be sloppy at all.
When preparing this chemical, all raw materials must be accurately weighed and mixed strictly according to the established ratio. Stirring speed, reaction temperature and time must be carefully controlled. A slight deviation in temperature, or excessive or insufficient reaction, affects the quality of the product.
Store in a cool, dry and well-ventilated place. This chemical is very sensitive to humidity and temperature. Humid environment or high temperature conditions can easily cause it to deteriorate. Packaging must also be tight to prevent contact with air and moisture.
When operating, the experimenter should be fully armed, wearing protective clothing, goggles and gloves to avoid contact with the skin and eyes. In case of inadvertent contact, rinse with plenty of water immediately and seek medical attention in time.
During use, ventilation equipment must be kept in good operation to prevent the accumulation of harmful gases. If a leak occurs, irrelevant personnel should be quickly evacuated and the leaked area should be properly disposed of. First absorb with inert materials, and then dispose of waste in accordance with relevant regulations.
The safety and operation specifications of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid are the key to ensuring the smooth experiment, personnel safety and environmental harmlessness, and must not be slack.

Application Area

4- (2 - Hydroxyerhyl) Piperazine - 1 - ethanesulfonic acid (4- (2 - Hydroxyerhyl) Piperazine - 1 - Erhanesulfonic Acid), this substance has a wide range of uses. In the field of biochemical experiments, it can be used as a buffer to stabilize the pH of the solution, maintain the stability of the experimental system, and make the reaction proceed in sequence, and the results are undoubtedly accurate. In medical research, cell culture often relies on it to create a suitable environment for cells and help them grow and reproduce. It is essential for exploring the mysteries of cells and studying the mechanism of diseases. It is also used in drug development to adjust the pH of the preparation, increase the stability and dissolution of the drug, and improve the efficacy of the drug. In chemical production, in a specific reaction system, it also adjusts the environment, promotes the smooth reaction, and improves the quality of the product. Its application in various fields is like a river in a boat, stable and far-reaching, indispensable.

Research & Development

Today, there is a thing named 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, which is of great research value in various chemical substances. Our generation takes the study and development as the service, and studies the properties and methods of making this thing.
Its properties are also, and it needs to be inspected in detail to observe its reaction in different situations to know whether it is stable and alive. The system seeks its precision and simplicity, and the materials used are easy and efficient. After repeated tests, I have obtained something.
The research of this thing is not only to clarify its current properties and systems, but also to expand its use. Thinking about it in various fields such as medicine and biochemistry, it may be possible to develop new paths and promote the progress of its industry. With time, diligent research, hope to achieve great success, contribute to the development of chemistry, apply it to the world, and benefit everyone.

Toxicity Research

Toxicity Study of 4- (2-Hydroxyethyl) Piperazine-1-ethanesulfonic Acid
This product, 4- (2-Hydroxyethyl) Piperazine-1-ethanesulfonic acid, is often used as a buffer in the field of biochemistry. However, the study of toxicity is related to the safety of users and the impact on the environment, which cannot be ignored.
At the beginning, animals were tested and fed with food containing this product. During menstruation, observe their posture and behavior. It is seen that the test animals may have decreased feeding and vitality, and some organs also showed slight changes.
At the cell level, this product was added to the cultured cells. After examination, cell growth and metabolism were disturbed, and the expression of specific genes was also abnormal.
In summary, although 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid has a wide range of biochemical uses, it is toxic. For subsequent use, be careful and study its safe dose carefully to ensure the safety of people and the environment.

Future Prospects

In the future, the research of chemical substances is not limited. Today, there is 4- (2-ethyl) piperazine-1-ethanesulfonic acid, which is widely used. In the field of biochemistry, it can improve the nature of the solution, and it can be used for many reasons.
Looking forward to the future, its development power can be improved. Or it can be used in new research and development to help improve the material, increase efficiency, and make patients benefit. With the development of biotechnology, it may be possible to make a big impact in the aspects of cell culture, improve the training parts, and promote good cell production.
Our researchers, diligent and refined, make it wonderful, so that this compound can be used in the future, and it can be used in the world for good.

Frequently Asked Questions

What is the chemical structure of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid?

4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, referred to as HEPES, has the following chemical structure:
HEPES is a zwitterionic buffer. Its chemical structure contains a piperazine ring, which is connected to a hydroxyethyl group at the 2 position of the piperazine ring and an ethanesulfonic acid group at the 1 position. Specifically, the piperazine ring is a hexamembered heterocyclic ring composed of two nitrogen atoms and four carbon atoms alternately. A hydroxyethyl group (-CH 2 CH 2 OH) is attached to the nitrogen atom at the 2 position. The oxygen atom in this hydroxyethyl group is covalently bonded to the nitrogen atom on the piperazine ring. The sulfur atom in the ethanesulfonic acid group forms a covalent bond with the nitrogen atom on the piperazine ring. This unique structure endows HEPES with good buffering properties, making it widely used in biochemical and cell biology experiments. It can effectively maintain the pH value of the solution, provide a suitable acid-base environment for biomolecules and cells, and reduce the impact of pH fluctuations on biological systems.

In which experiments is 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid commonly used?

4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) is a commonly used buffer in biochemical and cell biology experiments. It is frequently used in many experiments and is listed as follows:
1. ** Cell culture **: When cells are cultured in vitro, they are extremely sensitive to the pH value of the environment. Due to its good buffering ability, HEPES can stabilize the pH value of the cell culture medium in the range suitable for cell growth, usually 7.2-7.4. This can ensure the normal metabolism and physiological functions of cells. Processes such as cell adherent growth and proliferation benefit from a stable pH environment, preventing damage to cells due to pH fluctuations, and ensuring the smooth progress of cell culture experiments.
2. ** Enzyme activity determination **: Many enzymatic reactions require strict pH values, and slight changes in pH may affect enzyme activity. HEPES can create and maintain specific pH conditions to make enzymes in the best active state. For example, in some protein enzyme activity determination experiments, the HEPES buffer system is used to precisely regulate the pH value of the reaction system, so as to accurately determine the ability of enzymes to catalyze substrate reactions under specific conditions, providing a stable environment for studying the characteristics and functions of enzymes.
3. ** Protein crystallization **: The protein crystallization process requires a suitable and stable solution environment. HEPES can adjust the pH value of the solution while maintaining stable conditions such as ionic strength, which helps protein molecules to arrange in an orderly manner and form crystals. This is of great significance for analyzing the three-dimensional structure of proteins through X-ray crystallography. Only by obtaining high-quality protein crystals can accurate protein structure information be obtained, which helps to further study protein functions and mechanisms of action.

What is the pH buffer range for 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid?

If you want to know the pH buffer range of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid buffer, it should be noted that this is a commonly used buffer for biochemical experiments. Its buffering capacity is quite good, and the application range is specific. The pH buffer range of this buffer is about 6.8 to 8.2.
Guy 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid has a specific chemical structure and acid-base properties. In this pH range, the pH of the solution can be effectively maintained by the absorption and release of protons. In many biochemical and molecular biology experiments, the activity and stability of biological macromolecules such as proteins and enzymes are quite sensitive to environmental pH. The pH buffer range of this buffer is suitable for simulating the pH of most organisms in vivo, and can create a stable environment for biomolecules to maintain normal activity and function.
Therefore, the pH buffer range of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid buffer is approximately 6.8 to 8.2, which is of great significance in related experimental applications.

How is 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid stored?

If you want to store 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, you should do it properly according to the ancient method. This agent likes coolness but is afraid of heat. It should be placed in a shady place, away from direct sunlight, to prevent its quality from changing due to heat.
Its place should be dry and do not allow moisture to invade it. Cover moisture can easily lead to deliquescence and damage its original properties. If stored in a closed container, it can prevent contact with outside air and avoid oxidation and other changes.
When taking it, you should also be cautious. Purify your hands first, then take them with clean instruments, seal them as quickly as possible, and return them to their original storage. In this way, the agent will not be damaged for a long time, so that it can be used for future needs, and it can be used to the best of its ability.

What are the precautions for 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid?

When processing 4- (2-cyanoethyl) piperidine-1-glycolic acid, the following points should be paid attention to:
First, it is related to the purity of the material. Cyanoethyl, piperidine, glycolic acid and other raw materials must meet the purity standards. Too many impurities are easy to cause side reactions, which seriously affect the quality and yield of the product. If the raw material contains impurities, it may compete with the main reactants for the activity check point during the reaction process, generating unexpected by-products, thereby reducing the output of the target product.
Second, the reaction conditions are crucial. In terms of temperature, this reaction is quite sensitive to temperature. If the temperature is too high, the reaction rate will be accelerated, but the side reactions will also be intensified, or the product decomposition, polymerization and other conditions will occur; if the temperature is too low, the reaction rate will be delayed, the reaction time will be greatly extended, and even the reaction cannot be fully carried out. For example, if the reaction needs to be carried out in a specific temperature range, if it deviates from this range, the quality and yield of the product will be affected. Furthermore, the reaction pressure cannot be ignored. Some steps may need to be carried out under specific pressures, and improper pressure control will also cause adverse consequences to the reaction.
Third, the choice of solvent should not be underestimated. A suitable solvent can not only promote the dissolution of the reactants and make the reaction proceed smoothly in the homogeneous system, but also have an effect on the reaction rate and selectivity. Different solvents have different properties such as polarity and solubility, which have a great impact on the reaction process. Improper solvents may not be able to effectively dissolve the reactants, or interfere with the reaction mechanism, which ultimately affects the formation of the product.
Fourth, safety protection should not be ignored. Cyanoethyl-related compounds are toxic and irritating. When operating, safety procedures must be strictly followed, and complete protective equipment must be worn, such as protective clothing, gloves, protective glasses, and gas masks. The experimental site also needs to have good ventilation facilities to discharge the toxic gases that may be generated in time to prevent the poisoning of operators. At the same time, the disposal of waste materials should also meet environmental protection requirements to avoid pollution to the environment.
Fifth, the monitoring of the reaction process is indispensable. With the help of analytical methods such as thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), the reaction process can be monitored in real time, and the progress and degree of the reaction can be accurately grasped, so as to adjust the reaction conditions in time to ensure that the reaction is advancing in the direction of generating the target product and improve the purity and yield of the product.