What are the main applications of 4- (2 - Hydroxyethyl) -1-Piperazineethanesulfonic Acid and Disodium Salt?
4- (2-hydroxyethyl) -1-piperazine ethanesulfonate disodium salt, which is a commonly used reagent in biochemical research. It has important applications in many fields.
In the field of cell culture, it plays a key role. Cell culture requires a suitable pH environment, and this reagent can effectively maintain the stability of the pH value of the culture medium. Cells are quite sensitive to pH changes, and small fluctuations may affect their growth, metabolism and function. With this reagent, pH is delicately regulated to create a stable living environment for cells and ensure normal cell growth and reproduction, just like creating a stable habitat for cells.
In the study of enzymatic reactions, it is also indispensable. The activity of enzymes requires strict reaction conditions, and pH value is one of the key factors. The reagent can ensure the stability of the pH value of the reaction system, so that the enzyme is in the best active state, so that the enzymatic reaction can proceed smoothly, just like laying a smooth path for the operation of enzymes.
In the field of protein research, it also plays an important role. The structure and function of proteins are significantly affected by environmental factors, and pH value is an important factor. This reagent can stabilize the pH of the system, prevent proteins from denaturing due to pH fluctuations, and maintain their natural structure and function, just like building a protective barrier for proteins.
In summary, 4- (2-hydroxyethyl) -1-piperazine ethanesulfonate disodium salt plays a crucial role in cell culture, enzymatic reaction research, protein research and other biological related fields, providing strong support for the smooth development of scientific research.
What are the physicochemical properties of 4- (2 - Hydroxyethyl) -1-Piperazineethanesulfonic Acid, Disodium Salt?
4- (2-hydroxyethyl) -1 -piperazine ethanesulfonate disodium salt is a commonly used biochemical reagent. Its physicochemical properties are unique and crucial in the field of biochemical research.
This substance has stable properties and is mostly white crystalline powder under normal conditions, which is easy to store and use. In terms of solubility, it can be better dissolved in water to form a clear solution, which is conducive to various experimental operations. This property makes it widely used in experimental links such as buffer preparation, which can ensure that the reagent is evenly dispersed in the solution system and give full play to its role.
From the perspective of pH, this substance has good buffering properties and can effectively maintain a specific pH range of the solution. In many biochemical reactions, a suitable pH environment is the key to the smooth progress of the reaction. 4- (2-hydroxyethyl) -1 -piperazine ethanesulfonate disodium salt relies on this characteristic to ensure the stability of the pH of the reaction system and avoid interference due to pH fluctuations, laying the foundation for the success of the experiment.
In addition, its chemical stability is quite high, and it is not easy to react violently with common chemicals under normal conditions. This makes it possible to coexist with a variety of reagents in the same system without affecting its own and other reagents' performance. It provides convenience for the construction of complex biochemical experimental systems, and can cooperate with many biomolecules, enzymes, etc., to help researchers delve deeper into the mysteries of life processes.
4- (2 - Hydroxyethyl) -1-Piperazineethanesulfonic Acid, Disodium Salt What are the precautions during storage?
4 - (2 -hydroxyethyl) -1 -piperazine ethanesulfonate disodium salt, when storing, there are several urgent matters to pay attention to.
This compound is very hygroscopic, so it must be stored in a dry place to prevent moisture decomposition. The humidity of the storage environment should be strictly controlled, usually 40% - 60% relative humidity. If the humidity is too high, it may agglomerate due to moisture absorption, affecting quality and use.
Temperature is also a key factor. Generally speaking, it should be placed in a cool place and refrigerated at 2-8 ° C. If the temperature is too high, it may cause chemical instability and cause adverse reactions such as decomposition.
In addition, it is necessary to avoid mixing with oxidizing agents, acids, bases and other substances. Due to its chemical structure, it is easy to react with the above substances and damage its own purity and performance.
In addition, it should be kept away from fire and heat sources to prevent fire and the deterioration of compounds due to high temperature. The storage place should also ensure good ventilation to avoid the accumulation of harmful gases. In this way, 4 - (2-hydroxyethyl) -1 - piperazine ethanesulfonate disodium salt can be properly stored to maintain its quality and performance stability.
What is the synthesis method of 4- (2 - Hydroxyethyl) -1-Piperazineethanesulfonic Acid, Disodium Salt?
The synthesis method of 4 - (2 - hydroxyethyl) -1 - piperazine ethane sulfonate disodium salt, which is detailed as follows.
First of all, the raw materials should be prepared, 2 - hydroxyethyl piperazine and appropriate sulfonating reagents should be used. Sulfonating reagents, such as 1,2 - dichloroethane sulfonic acid, should be selected carefully to meet the needs of the reaction.
In the reactor, pour an appropriate amount of 2 - hydroxyethyl piperazine, according to the number of moles, and add the sulfonating reagent in a precise way. Reaction temperature, when controlled at an appropriate value. Initially, it can be slowly heated to about 50 - 60 degrees Celsius, so that the two gradually interact. At this temperature, the molecular movement is appropriate, which is conducive to the initiation of the reaction and avoids the proliferation of side reactions.
As the reaction proceeds, the temperature can be gradually raised to 80-90 degrees Celsius to accelerate the reaction process. The reaction time is about time, and it needs to be measured by an instrument during this period to observe the degree of reaction.
After the reaction is completed, the temperature is cooled to room temperature. At this time, the reaction solution contains products and impurities. By means of neutralization, a sodium hydroxide solution can be slowly dripped in, and the pH is adjusted to alkaline, so that the product becomes a sodium salt state.
Then, the separation technique is performed. It can be extracted with an organic solvent to remove its oil-soluble impurities. Then by vacuum distillation, in addition to the solvent, the crude product is obtained.
The crude product is impure and needs to be refined. An appropriate solvent can be selected by recrystallization, such as ethanol-water mixed solvent. Dissolve the crude product in a hot solvent and filter it hot to remove its insoluble matter. After the filtrate is cooled, the product crystallizes out, filtered and dried to obtain a pure 4- (2-hydroxyethyl) -1 -piperazine ethane sulfonate disodium salt. The whole process, the operation should be careful, and the conditions should be strict, in order to obtain a good product.
How do 4- (2 - Hydroxyethyl) -1-Piperazineethanesulfonic Acid, Disodium Salt react with other compounds?
The disodium salt of 4- (2-hydroxyethyl) -1 -piperazine ethanesulfonate, often referred to as "HEPES disodium salt", has a wide range of uses in biochemical experiments. Its properties are relatively stable, and it will react when it encounters specific substances.
In contact with strong acids, the sulfonic acid group in HEPES disodium salt binds to protons, and the equilibrium shift, and the pH of the solution changes. In case of hydrochloric acid, the reaction is intense, and the degree of ionization is greatly changed, which affects the buffering performance.
In case of strong bases, the nitrogen atom on the hydroxyethyl group or piperazine ring can interact with the hydroxide group. Like sodium hydroxide, or dehydroxy of hydroxyethyl, affecting the molecular structure and function.
Metal ions such as copper ions and zinc ions can coordinate and complex with nitrogen and oxygen atoms in HEPES disodium salt, changing molecular properties, or reducing buffering capacity and interfering with experiments.
When encountering strong oxidants, the molecule contains oxidizable elements such as nitrogen and sulfur, or is oxidized, resulting in structural breakdown and dysfunction. In case of hydrogen peroxide, the sulfur oxidation state may rise and the molecular chemistry may be changed.
Organic halide, or nucleophilic substitution with HEPES disodium salt, the halogen atom is replaced with active hydrogen or other groups in the molecule, changing the chemical structure and properties.
Experimental HEPES disodium salt, be careful to pay attention to its possible reaction with other substances to prevent affecting the results, and ensure the accuracy and reliability of the experiment.
