4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid

Lingxian Chemical

Specifications

HS Code	170907
Chemical Formula	C8H18N2O6S
Molar Mass	282.304 g/mol
Appearance	White to off - white crystalline powder
Solubility	Soluble in water
Ph Range	6.8 - 8.2 (useful buffer range)
Pka	7.55 at 25 °C
Density	1.382 g/cm³ (approximate)
Melting Point	234 - 237 °C
Stability	Stable under normal conditions
Application	Used as a biological buffer in biochemical and cell culture applications

Packing & Storage

Packing	1 kg of 4-(2 - Hydroxyethyl)-1 - Piperazineethanesulfonic acid packaged in a resealable bag.
Storage	4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic acid (HEPES) should be stored in a cool, dry place, away from heat sources and direct sunlight. Keep it in a tightly closed container to prevent moisture absorption. Store at room temperature in a well - ventilated area, ensuring the storage environment is free from incompatible substances that could potentially react with it.
Shipping	4-(2 - Hydroxyethyl)-1 - Piperazineethanesulfonic acid is carefully packaged to prevent spillage. Shipping is via reliable carriers, ensuring proper handling and temperature control for this chemical's safe transport.

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4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid

General Information

Historical Development

4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid first appeared in the field of scientific research. After years, its development path can also be traced. In the past, scientific researchers were exploring, hoping to obtain excellent reagents. At that time, the technology of chemistry was progressive, and everyone studied various compounds painstakingly.
After many attempts, 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid emerged. Because of its unique nature, it is useful in biochemical experiments, cell culture and many other aspects. With the deepening of research, its application scope has become wider, contributing to the progress of scientific research and assisting a lot. It has gradually become indispensable in the fields of chemistry and biology. Its development process has also witnessed the journey of scientific research progress.

Product Overview

4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid, this is the chemical I have recently researched. Its mild nature and wide range of uses in biochemical experiments.
Viewed its shape, it is often a white crystalline powder, easily soluble in water, and the properties of the solution are stable. In biochemical research, it is often used as a buffer to maintain the pH balance of the system. Because it has little interference with the biological system, it can ensure the suitability of the experimental environment and make many biochemical reactions proceed smoothly.
When preparing, it is necessary to carefully control all aspects, from the selection of raw materials to the control of reaction conditions. In this way, high-quality products can be obtained, paving the way for scientific research.

Physical & Chemical Properties

The physicochemical properties of 4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid are particularly important. Looking at its shape, it is a white crystalline powder under normal conditions, like fine snow, pure and uniform in quality. Its solubility is good, it can be well dissolved in water, just like fine sand entering the flow, and it is immiscible.
When it comes to stability, under normal conditions, it is quite stable and difficult to change, just like a rock. Its melting point is also fixed, and under a specific temperature, there is a change in the state of matter. Due to its unique physical and chemical properties, this substance is used in many fields, either as a buffering agent or in biochemical research, helping to explore the mysteries of life. Its function is significant, and it is one of the important existences in the field of chemical research.

Technical Specifications & Labeling

4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid is also a chemical product. Its process specifications and identification (commodity parameters) are of paramount importance. Looking at its quality, its color is pure and its properties are stable, which is the first standard. The preparation method requires strict procedures, and the selection of raw materials, the control of heat, and the degree of time are all related to quality. In the identification, the name matches the truth, and the label is accurate. The ingredients, properties, uses, and storage methods contained in it are clear one by one. By following this process specification and identification, you can get good products, which can be used in the chemical industry, and make the best use of them. There is no risk of error, so as to meet the needs of all parties.

Preparation Method

The raw material of 4 - (2 -hydroxyethyl) -1 -piperazine ethanesulfonic acid is the key to the production process, reaction steps and catalytic mechanism. First take an appropriate amount of piperazine, with a certain amount of 2-chloroethanol, and mix the two in the reactor in an appropriate proportion. At a suitable temperature and pressure, the two can fully react. This is the beginning of the reaction step. During the reaction, a specific catalyst is added to accelerate the reaction process and optimize the catalytic mechanism to improve the purity and yield of the product. The raw materials used need to be carefully selected to ensure high quality, which is the importance of raw materials. After the reaction is completed, the impurities are removed through several fine purification processes to obtain a pure product of 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid, which is a complete production process.

Chemical Reactions & Modifications

Taste the industry of chemistry, explore the changes of matter, in order to improve. Today there is 4- (2 -Hydroxyethyl) -1 -Piperazineethanesulfonic acid, which is related to chemical reactions and denaturation, and is particularly important.
The wonders of its reaction, if you want to solve the cow, you need to gain insight into the details. Chemists use ingenuity and skills to observe the structure of its molecules and explore the laws of its reactions. If you encounter difficulties, such as sailing against the current, you will not be discouraged.
On the way to denaturation, chemists study carefully, hoping to change its nature and increase its effectiveness. Just like carving beautiful jade, carefully pondering. After countless trials, or a good method, this chemical has better performance, wider application, and added to the chemical industry, benefiting many fields.

Synonyms & Product Names

4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid has many aliases, and in various ancient records, it also has different names. This substance is often used in the industry under a variety of trade names.
From its name, or from its structural characteristics, or according to its properties and uses, various aliases are derived. In the fields of pharmacy and chemistry, it has an important position, so many parties have different expressions on its naming.
or cloud "hydroxyethylpiperazine ethanesulfonic acid", and it is also called "HEPES", which are all common aliases. And its trade names also vary with the needs of manufacturers and markets. Although the names are different, they all refer to the same chemical substance, which plays a unique role in scientific research and industrial production.

Safety & Operational Standards

4 - (2 - hydroxyethyl) - 1 - piperazine ethanesulfonic acid, this is a chemical substance. In our field of chemical research, its safety and operating practices are of paramount importance.
When handling this substance, it should be carried out in a well-ventilated place. Experimenters must wear appropriate laboratory clothes, protective gloves and goggles to prevent it from coming into contact with skin and eyes. In case of inadvertent contact with skin, rinse with plenty of water immediately. If the condition is serious, seek medical treatment; if it comes into contact with eyes, rinse with plenty of water quickly and seek professional medical assistance as soon as possible.
Store in a cool, dry and well-ventilated place, away from sources of fire and oxidants. It needs to be stored separately from other chemicals to avoid reaction.
During use, strictly follow the established operating procedures. Weighing should be accurate, and when configuring the solution, pay attention to its dissolution conditions and concentration requirements. After use, properly dispose of the remaining substances and must not be discarded at will to avoid pollution to the environment.
Only by strictly following these safety and operating standards can we ensure the safety of the research process, and also ensure the accuracy and reliability of the research results, so that our research on 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid can be smoothly advanced.

Application Area

4- (2 -hydroxyethyl) -1 -piperazine ethanesulfonic acid is useful in various fields.
In the field of medicine, it is often used as a biological buffer to stabilize the acid and alkali of the solution, protect the properties of biological molecules, in the liquid of cell culture, and in the agent of biochemical experiments. It is indispensable to maintain the normal state of cells and molecules, and help the experiment to be accurate and reliable.
In the industry, it is also used in papermaking, coatings and other industries. In papermaking, it controls the acid and alkali of pulp and the quality of paper products; in coatings, it stabilizes the acid and alkali of the system and ensures the excellent performance of coatings.
Scientific research category, which is a commonly used reagent, used in protein, enzyme and other research, to help scientists explore biological mysteries and understand the mechanism of life.
From this perspective, 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid is widely used in many fields such as medicine, industry, scientific research, etc., and has important functions. It is the key to promoting the development of all parties.

Research & Development

Today, there is a product named 4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid, which is quite important in the field of biochemistry. We are responsible for the researcher to investigate the properties, performance and use of this product.
Initial observation of its properties, we know that it is an excellent buffer, which can stabilize the pH of the system and ensure the smooth reaction in biochemical experiments. Then explore its use, which is indispensable in cell culture, protein research and other fields, and the researcher obtains accurate results.
We are working tirelessly to explore its use and increase its effectiveness. After years of work, it has been beneficial to the synthesis method and the purification technique. In the future, this product can be developed in more fields, promoting the progress of biochemical research, and contributing to the rise of science.

Toxicity Research

In this world, I have studied a thing named 4- (2 - Hydroxyethyl) -1 - Piperazineethanesulfonic acid. The toxicological study of this thing is related to the public.
Observe the way of poison in the past, and observe its effect on living beings. To understand the toxicity of this acid, we should try it on all living beings. Observe its entrance, skin, or inhalation of qi, and observe changes in the body shape, behavior, and viscera of living beings.
If living beings eat it, and see weakness, reduced food, body loss, or viscera damage, or blood disorder, we know that its toxicity is harmful. And look at its contact with the skin, if the skin is red, swollen, or painful, it is also poisonous. If inhaling it causes coughing, asthma, and difficulty breathing, the toxicity is obvious.
Study the toxicity of this product, and we can know that it is safe in the world.

Future Prospects

Guanfu 4- (2 - Hydroxyethyl) -1 - Piperazineethanesulfonic acid has shown its use in this world. However, our generation regards it as a great hope for the future.
Its properties are stable, and in the field of biochemistry, it is often used as a buffer agent to protect the environment and protect the nature of substances. However, in the future development, we should seek its purity, increase its effectiveness, and expand its use. To make this agent more effective in subtle research, such as cell analysis and gene research.
And hope that the method of its preparation will tend to be simpler and reduce its consumption, which is in line with heaven and nature. In this way, this agent will be as bright as the stars on the way to the future, assisting the biochemical industry, reaching an unprecedented level, and becoming the work expected by everyone.

Frequently Asked Questions

What is the chemical structure of 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid?

4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid, or HEPES, has the following chemical structure:
This compound contains a piperazine ring structure, which is connected to a 2-ethanesulfonate group at position 1 of the piperazine ring and a 2-hydroxyethyl group at position 4. Structurally, the piperazine ring, as a core skeleton, has certain rigidity and stability. Two nitrogen atoms enable the piperazine ring to participate in a variety of chemical interactions, such as binding to protons, thus exhibiting buffering capabilities. The existence of 2-ethyl sulfonate group endows the molecule with good water solubility. Sulfonic acid group (-SO-H) is a strong acidic group, which can partially ionize hydrogen ions in water, and its conjugated base has a certain stability, which plays a key role in regulating the pH of the solution. 2-hydroxyethyl group introduces hydrophilic hydroxyl group (-OH) to the molecule, which not only enhances the water solubility of the molecule, but also interacts with surrounding water molecules or other polar molecules through hydrogen bonds, further affecting the behavior and properties of the molecule in solution.
This structural property makes 4- (2-hydroxyethyl) -1 -piperazine ethanesulfonic acid a commonly used buffer in biochemistry and molecular biology experiments, capable of maintaining a relatively stable pH in a wide pH range, providing a suitable acid-base environment for biomacromolecule activity research, cell culture and other experiments.

Which experimental systems are commonly used for 4- (2-hydroxyethyl) -1-piperazinethanesulfonic acid?

4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid (HEPES) is commonly used in a variety of biological experimental systems such as cell culture, protein crystallization, enzymatic reactions, etc. The following is your explanation:
Cell culture is the cornerstone of biomedical research, and HEPES is crucial in this field. Cells are extremely sensitive to the culture environment, and even small changes in pH can affect their growth, metabolism, and function. HEPES has a strong buffering ability, which can stabilize the pH value of the cell culture environment within a suitable range, usually 7.2-7.4, just like building a strong "acid-base barrier" for cells, ensuring that cells can proliferate and differentiate normally as if they were in a "comfortable home", greatly improving the success rate and stability of cell culture, and laying a solid foundation for subsequent various cell-related studies.
Protein crystallization experiments are dedicated to analyzing the three-dimensional structure of proteins, which is the key to understanding the function and mechanism of protein action. During the experiment, factors such as the pH value of the solution and the ionic strength have a profound impact on the formation of protein crystallization. HEPES can precisely regulate the pH value of the solution, and has good biocompatibility with proteins. It does not interfere with the structure and activity of proteins. It is like a "gentle guardian", which helps proteins to arrange in an orderly manner in a suitable environment, and then form high-quality crystals. It is possible to analyze the structure of proteins through X-ray crystallography and other technologies.
Enzymes are efficient catalysts for various chemical reactions in organisms, and their activity requires strict reaction conditions. In an experimental system that simulates enzymatic reactions in vivo, HEPES can create pH conditions similar to the physiological environment, so that the enzyme can maintain the best active conformation and give full play to catalytic efficiency. For example, many enzymes involved in metabolic pathways can efficiently catalyze the conversion of substrates in the HEPES buffer system, providing a reliable environment for studying the kinetics and mechanism of action of enzymes and screening for inhibitors or activators of enzymes.
In summary, 4 - (2-hydroxyethyl) -1 - piperazine ethanesulfonic acid (HEPES) has become an indispensable key reagent in many biological experimental systems due to its unique buffering properties and biocompatibility, just like a "right-hand man" in the journey of biological research, promoting life science research to a new height.

What is the pH buffer range of 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid?

To know the pH buffer range of 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid buffer, it is necessary to know the characteristics of this buffer. 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid, often referred to as HEPES, is a commonly used buffer in biochemical and molecular biology experiments.
HEPES has many advantages. Its chemical properties are stable, it is less affected by changes in temperature and concentration, and it has little interference with chemical reactions in vivo.
Its pH buffer range is about 6.8 to 8.2. This range allows it to effectively maintain the pH stability of the system in a variety of biological experiments. For example, in cell culture experiments, most mammalian cells are suitable for growing at pH 7.2-7.4, and HEPES buffer can ensure that the pH value of the culture medium is stable in this range, creating good conditions for cell growth. For some enzymatic reactions, the specific pH value is the best state of enzyme activity, and HEPES buffer can control the pH value of the system within a suitable range according to the needs of the reaction to ensure the smooth progress of enzymatic reactions.
Therefore, the pH buffer range of 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid buffer is about 6.8-8.2, which is widely used in the field of biological experiments due to its characteristics.

What are the precautions for 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid during storage?

In the process of storing 4- (2-methoxy) -1-naphthalene acetyl acid, there are many things to pay attention to. This compound has special properties and is sensitive to environmental conditions.
Control of the first degree. It is suitable to be stored at a low and low temperature. Generally speaking, it is better to be at 2 to 8 degrees Celsius. If the temperature is high, it is easy to cause the reaction, causing it to change and reduce the activity. If the temperature is high, or the molecular parts can be affected, the original equilibrium will be broken, so that the effect will be weak, or even ineffective.
Secondly, the temperature should not be ignored. Maintain the dry environment, because it is susceptible to moisture. The temperature in the air is too high, and the crystal lattice of water molecules or compounds can lead to hydrolysis reaction. Once hydrolyzed, the molecule is cracked, and the chemical properties are changed. Therefore, there are good moisture-proof measures in place, such as placing it in a drying device, or adding a dry layer.
Furthermore, light will also dry its properties. This compound is sensitive to light, and under light irradiation, it may react to actinic reactions. Light quantum acts on the molecule, causing the particles to break down, causing chemical cracking or rearrangement. Therefore, when stored in the dark, brown bottles or opaque containers can be used to reduce light and shadow.
In addition, it is necessary to avoid contact with other chemical substances. Due to its chemical activity, or the reaction of certain substances. Such as oxidation and original destruction, it may destroy its molecules. Therefore, it needs to be stored well, and the storage area should be kept clean to prevent the mixing of oil.
Therefore, the storage of 4- (2-methoxy) -1-naphthalene acetyl acid requires the control of the degree, temperature, light and the avoidance of contact with other substances in order to maintain its chemical properties and maintain good efficacy.

How is the solubility of 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid?

What is the solubility of 4 - (2 - hydroxyethyl) - 1 - piperazine ethanesulfonic acid? This is a question about the chemical properties, and let me explain it in the words of the ancients.
Fu 4 - (2 - hydroxyethyl) - 1 - piperazine ethanesulfonic acid, often used as a biochemical experimental buffer. Its solubility is related to the success or failure of many experiments. This substance has good solubility in water. Because of its molecular structure, it contains hydrophilic groups, such as hydroxyethyl and sulfonic acid. These two interact with water molecules, and by the force of hydrogen bonds, the molecules are uniformly dispersed in water to form a stable solution. The practical application of
concept is in the construction of buffer systems for biochemical experiments. The good solubility of this substance ensures that it can be quickly and uniformly integrated into the reaction system, and the pH of the solution can be precisely regulated. Under suitable temperature and stirring conditions, its dissolution rate is particularly fast, and it can reach the required concentration in a short time. And the solution stability is quite good. In common experimental environments, it can be maintained for a long time without precipitation or deterioration.
However, it should be noted that although it has good solubility in water, in organic solvents, the solubility is quite different. In case of organic solvents with strong hydrophobicity, it is difficult to dissolve them because of the weak interaction between hydrophilic groups and organic solvents. This property also limits its application scenarios, and must be carefully considered when selecting solvents and reaction systems.
In short, 4- (2-hydroxyethyl) -1-piperazine ethanesulfonic acid has good solubility in water, which makes it in the buffer field of biochemical experiments. It plays a crucial role, but the solubility of organic solvents depends on the specific solvent properties.