What is 4- (2-Hydroxyethyl) -1-Piperazinepropanesulfonic Acid?
4- (2 -Hydroxyethyl) -1 -Piperazinpropanesulfonic acid is a commonly used buffer in biochemical research. Its English name is 4- (2 -Hydroxyethyl) -1 - Piperazinepropanesulfonic Acid, often referred to as HEPES.
Looking at its properties, it is mostly white crystalline powder, soluble in water, and can effectively maintain a certain pH in solution. Its buffer range is quite suitable, about pH 6.8-8.2, which is similar to the acid-base range of many organisms in the body environment, so it is widely used in cell culture, enzyme reaction and other biochemical experiments.
In the field of cell culture, cell growth is extremely sensitive to the pH of the environment, and small pH changes may affect cell growth, metabolism and even survival. HEPES buffers can create a stable acid-base environment for cells to ensure normal growth and reproduction under suitable conditions. In the enzyme reaction system, the activity of enzymes is also closely related to the pH of the environment. HEPES can maintain the pH stability of the reaction system, allowing enzymes to exert the best activity and help the reaction proceed smoothly.
In addition, HEPES is stable in nature, non-toxic and irritating to organisms. In complex biochemical systems, it is not easy to have adverse reactions with other substances. Therefore, it is favored by biochemical researchers and has become an indispensable reagent for maintaining the acid-base balance of biochemical systems.
What are the main uses of 4- (2-Hydroxyethyl) -1-Piperazinepropanesulfonic Acid?
4- (2-hydroxyethyl) -1 -piperazinpropanesulfonic acid, often referred to as HEPPS, is a crucial buffer in biochemical research and is widely used in many fields.
First, in biochemistry and molecular biology experiments, it plays a significant role. In the study of proteins and nucleic acids, protein electrophoresis, nucleic acid extraction and amplification experiments are extremely sensitive to environmental pH. HEPPS can stabilize the pH value of the reaction system within a specific range due to its excellent buffering ability, ensuring the stability of the structure and function of proteins and nucleic acids, and will not be denatured or inactivated due to large fluctuations in pH. Just like PCR amplification of DNA, a suitable and stable pH environment is the guarantee for the efficient activity of Taq DNA polymerase, and HEPPS can create and maintain such suitable pH conditions.
Second, the field of cell culture is also inseparable from it. When cells are cultured in vitro, they have strict requirements on the pH value of the environment in which they are located. HEPPS can effectively regulate the pH value of the cell culture medium, create a stable and reliable living environment for cells, and effectively promote the normal growth, proliferation and metabolism of cells. Like mammalian cell culture, the suitable pH range for cell growth is narrow, and HEPPS can stabilize the pH range of the culture medium and prevent cells from growth arrest or even death due to abnormal pH.
Third, it is also indispensable in the study of enzymatic reactions. The activity of enzymes is closely related to the pH of the reaction system, and different enzymes often have their specific optimum pH value. HEPPS can precisely adjust and stabilize the pH value of the reaction system, so that the enzyme is in the best active state, thus helping researchers to deeply explore the catalytic mechanism and kinetic parameters of enzymes. For example, when studying the hydrolysis of some proteases, HEPPS can be used to maintain a stable pH, so that the activity changes of proteases can be clearly observed under different conditions.
What are the physicochemical properties of 4- (2-Hydroxyethyl) -1-Piperazinepropanesulfonic Acid?
4- (2-hydroxyethyl) -1 -piperazinpropanesulfonic acid, its physical state is white crystalline powder at room temperature. Melting point is between 238-240 ℃, this characteristic makes it have a specific transition temperature during heating. In terms of solubility, it is easily soluble in water to form a clear and transparent solution, but it has poor solubility in common organic solvents such as ethanol and ether.
In terms of pH, the pKa value is about 7.5-7.7, which determines its key role in the buffer system, which can effectively maintain the pH value of the solution in a specific range. And the chemical properties of the substance are stable, it is not easy to react with common chemicals under normal conditions, has good thermal stability, and is difficult to decompose at high temperatures, ensuring the reliability of use in a variety of environments. At the same time, its biocompatibility is excellent, and it has minimal interference to biological systems when applied in biological experiments, medical treatment and other fields, providing great convenience for related research and applications.
What should I pay attention to when storing and transporting 4- (2-Hydroxyethyl) -1-Piperazinepropanesulfonic Acid?
4- (2-hydroxyethyl) -1-piperazinopropanesulfonic acid, or HEPPS, is a commonly used buffer for biochemical experiments. When storing and transporting it, pay attention to many aspects.
When storing, the first ambient temperature. It should be placed in a cool place. Generally speaking, the temperature is preferably 2-8 ° C. Due to high temperature, or its chemical properties change, affecting the buffering performance. If the temperature is too low, or the material crystallizes, it is difficult to dissolve during use, resulting in experimental errors.
Secondly, the ambient humidity should not be ignored. It should be stored in a dry place to prevent moisture. Once it gets wet, it may cause reactions such as hydrolysis, which will change its purity and performance.
Furthermore, it needs to be stored away from light. The energy in the light may cause photochemical reactions to occur, destroying the molecular structure and making the buffer ineffective.
When transporting, ensure that the packaging is intact. Because the substance is mostly powder or crystalline, damage to the packaging is prone to leakage, which is both wasteful and pollutes the environment. And the temperature and humidity during transportation also need to be controlled to avoid extreme conditions affecting its quality.
In addition, it should be transported separately from other chemicals. Because of its specific chemical properties, it comes into contact with certain substances or reacts dangerously. In short, the storage and transportation of HEPPS should adhere to the conditions and ensure its quality, which is the foundation for the accuracy and success of the experiment.
What is the approximate market price range for 4- (2-Hydroxyethyl) -1-Piperazinepropanesulfonic Acid?
4- (2-hydroxyethyl) -1 -piperazinpropanesulfonic acid, often referred to as HEPES, is a commonly used zwitterionic buffer in biochemical research. Its market price range varies significantly due to factors such as quality, purity, packaging specifications and suppliers.
Looking at past market conditions, small packages (such as 25 grams) analyze pure grade products, and the price per package may be between 30 and 80 yuan. If it is chemically pure, the price is slightly lower, or 20 to 50 yuan per package.
As for larger packages (such as 500 grams), high purity products, the price per bottle may be 300 to 800 yuan. Ordinary industrial grade, 500 grams package or 150 to 400 yuan.
However, the market is changing, and the price fluctuations of raw materials, production process improvements, and changes in market supply and demand can all cause their prices to fluctuate. When purchasing, when consulting different suppliers and carefully observing market dynamics, you can get affordable and suitable products.
