What is the chemical structure of 4- (2 - Hydroxyethyl) Piperazine-1- (2 - Hydroxy Propane sulfonic Acid)?
4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid), this is a rather complex organic compound. Its chemical structure is cleverly spliced from several parts.
Looking at its structure, the piperazine ring is the core skeleton. The piperazine ring is a six-membered heterocycle containing two nitrogen atoms, with unique electron cloud distribution and chemical activity. At the 4th position of the piperazine ring, 2-hydroxyethyl is chemically bonded. This hydroxyethyl group is a hydrophilic group, connected by ethyl and hydroxyl. The presence of the hydroxyl group endows the compound with certain polarity and hydrophilicity, which can participate in the formation of hydrogen bonds and affect the solubility and reactivity of the compound.
In the first position of the piperazine ring, 2-hydroxypropanesulfonic acid is connected. This part is also of great significance. The propanesulfonic acid group is a strongly acidic group. The sulfonic acid group (-SO-H) can ionize hydrogen ions in aqueous solution, making the compound acidic, and plays a key role in many chemical reactions and ion exchange processes. At the same time, hydroxyl groups are introduced into the propane chain connected to the sulfonic acid group to further increase the hydrophilicity of the molecule and the reaction check point.
The delicate design of this compound makes it have a variety of characteristics, and may have unique uses in the fields of biochemical research, buffer solution preparation, etc. Due to the stability of the piperazine ring, the hydrophilicity of hydroxyethyl and hydroxyl groups, and the acidity of sulfonic acid groups, it can meet the chemical and biological needs under specific conditions.
What are the main applications of 4- (2 - Hydroxyethyl) Piperazine-1- (2 - Hydroxy Propane sulfonic Acid)?
4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid), often referred to as HEPPS, is a biological buffer. It is mainly used in many fields, as detailed below:
In the field of biochemistry and molecular biology, this is a commonly used buffer. In the research experiments of biological macromolecules such as proteins and nucleic acids, operations such as protein separation, purification, crystallization, and nucleic acid amplification and sequencing require precise control of the pH of the reaction system. HEPPS can maintain the stability of the pH of the reaction system by virtue of its specific buffer range and excellent buffer capacity, avoid the impact of pH fluctuations on the structure and activity of biomacromolecules, and help obtain reliable experimental results.
The field of cell culture is also indispensable. When cells are cultured in vitro, they are extremely sensitive to environmental pH. HEPPS can effectively adjust the pH of cell culture medium, create a stable and suitable living environment for cells, promote normal cell growth and proliferation, and prevent cell physiological dysfunction or even death due to abnormal pH. It is one of the key substances to ensure the success of cell culture.
In the study of enzymatic reactions, the activity of enzymes is closely related to the pH of the reaction system. HEPPS can create a stable pH condition, so that the enzyme is in the best active state, which is helpful for in-depth exploration of the catalytic mechanism and kinetic parameters of enzymes, and lays a theoretical foundation for the application of enzymes in industry, medicine and other fields.
In addition, HEPPS also plays an important role in the biochemical detection items of clinical diagnosis. Some tests need to be carried out under specific pH conditions to ensure the accuracy and reliability of test results. HEPPS can meet this demand as a buffer and improve the accuracy of diagnosis.
What are the precautions for the use of 4- (2 - Hydroxyethyl) Piperazine-1- (2 - Hydroxy Propane sulfonic Acid)?
4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid), often used as a biological buffer, should pay attention to the following points when using:
First, pay attention to its pH buffer range. The buffer range of this substance is about 6.1-7.5, and it must be used within the suitable range to ensure the buffering effect. If it exceeds this range, the buffering capacity will be weakened and the pH stability of the system will not be maintained. For example, in a specific enzymatic reaction, the enzyme activity beyond the range may be affected, resulting in abnormal reactions.
Second, pay attention to solubility. It is easily soluble in water, and it should be stirred or heated to help dissolve when dissolving. However, the heating temperature should not be too high to prevent decomposition. If a high-concentration solution is prepared, it can be dissolved in warm water first to avoid uneven local concentration.
Third, pay attention to the influence of purity and impurities. Impurities or interfere with experiment and production. Choose high-purity products and check the quality before use. Like cell culture, impurities or toxic cells interfere with experimental results.
Fourth, pay attention to storage conditions. It should be stored in a cool and dry place, protected from light and cool, to prevent moisture absorption and deterioration. If improper storage causes deterioration, it will affect the system during use, such as reduced buffer performance and introduction of impurities.
Fifth, consider the compatibility with other substances. Coexist or react with metal ions, strong oxidants, etc. Make sure not to react adversely with substances in the system when using. In complex chemical systems, reactions may change the buffering properties and generate precipitates, which may affect the normal progress.
What are the physicochemical properties of 4- (2 - Hydroxyethyl) Piperazine-1- (2 - Hydroxy Propane sulfonic Acid)?
4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid) is a rather important chemical substance. Its physicochemical properties are unique.
In terms of physical properties, this substance is usually white crystalline powder with a fine and uniform appearance. It is quite stable at room temperature and pressure, but it also has certain sensitivity to environmental temperature and humidity and other factors. Its solubility is considerable, and it exhibits good solubility in water. It can dissolve with water in a suitable ratio to form a uniform and stable solution. This property makes it advantageous in many chemical processes and industrial applications involving aqueous solution systems.
In terms of chemical properties, 4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid) has a certain acid-base buffering ability. Because its molecular structure contains specific functional groups, it can effectively adjust the pH of the solution and maintain the acid-base balance of the system within a specific pH range. This buffering property is widely used in biochemical experiments, pharmaceutical preparations and other fields, such as in the process of cell culture, it can create an acid-base environment suitable for cell growth. At the same time, the hydroxyl and sulfonic acid groups in its molecules enable the substance to participate in many chemical reactions, such as esterification reactions, nucleophilic substitution reactions, etc., providing a broad space for its application in the field of organic synthesis.
In addition, the stability of 4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid) cannot be ignored. Under normal storage and use conditions, its chemical structure is not prone to significant changes, and it can maintain its inherent physical and chemical properties for a long time. This characteristic ensures its reliability and repeatability in different scenarios.
What are the advantages of 4- (2 - Hydroxyethyl) Piperazine-1- (2 - Hydroxy Propane sulfonic Acid) over other similar compounds?
4- (2-hydroxyethyl) piperazine-1- (2-hydroxypropanesulfonic acid), hereinafter referred to as HEPPS, does have many advantages compared to other similar compounds.
First, HEPPS has good buffering properties. Just like a rock-solid dam, in a specific pH range, it can effectively resist the impact of acid-base changes on the pH of the system and maintain the stability of the pH of the system. This property is extremely critical in many biochemical reactions and experiments, because the structure and function of most biomolecules are extremely sensitive to pH changes. For example, for many enzymatic reactions, enzymes can only exhibit the best activity under a suitable and stable pH environment. The existence of HEPPS can create such a stable environment to ensure the smooth progress of the reaction.
Second, HEPPS is chemically stable. Like an ancient object that has been baptized and not perishable over time, it is not easy to participate in chemical reactions under common chemical reagents and reaction conditions, and it is not easily decomposed. In this way, in complex experimental systems or industrial production processes, it will not interfere with the target reaction or product quality due to its own chemical changes, greatly improving the reliability and repeatability of experiments and production.
Third, HEPPS has good biocompatibility. Like a good friend who coexists in harmony with organisms, it is non-toxic and irritating to biological cells and tissues. This makes it useful in biomedical research, cell culture and other fields. In the process of cell culture, it can not only create a stable pH environment for cells, but also does not have adverse effects on physiological processes such as cell growth and metabolism, which helps researchers to more accurately study the physiological and pathological mechanisms of cells.
Fourth, HEPPS has good solubility. Just like salts that are highly soluble in water, it can be quickly and fully dissolved in water and a variety of organic solvents. This property is convenient for it to be used in different experimental and production scenarios, and different concentrations of solutions can be flexibly configured according to actual needs to meet diverse application needs.