4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Sodium Salt Buffer

Lingxian Chemical

Specifications

HS Code	337454
Name	4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Sodium Salt Buffer
Chemical Formula	C8H17N2NaO5S
Molecular Weight	260.28 g/mol
Ph Range	6.8 - 8.2
Pka	7.55 (at 25°C)
Appearance	White to off - white powder
Solubility	Soluble in water
Buffering Capacity	Effective buffering capacity within its pH range
Stability	Stable under normal storage conditions
Compatibility	Generally compatible with a wide range of biological molecules

Packing & Storage

Packing	100 - gram package of 4-(2 - Hydroxyethyl)Piperazine - 1 - Ethanesulfonic Acid Sodium Salt Buffer
Storage	Store 4-(2 - Hydroxyethyl)Piperazine - 1 - Ethanesulfonic Acid Sodium Salt Buffer in a cool, dry place away from direct sunlight and heat sources. Keep the container tightly sealed to prevent air and moisture absorption, which could affect its chemical stability and buffer capacity. If stored properly, it will maintain its quality for the specified shelf - life.
Shipping	4-(2 - Hydroxyethyl)Piperazine - 1 - Ethanesulfonic Acid Sodium Salt Buffer is typically shipped in well - sealed containers. Packaging safeguards against moisture and damage, ensuring the buffer arrives in proper condition for laboratory use.

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4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Sodium Salt Buffer

General Information

Historical Development

4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer, the evolution of its material, has its own origin. In the past, various sages pursued chemical research in order to obtain a suitable buffer agent. After years of study, detailed observation of physical properties, repeated trials of formulas. Over the years, this 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer was obtained. Its performance is excellent, and it is gradually used in various fields of biochemistry and medicine. At first, it was not widely used, but it was deepened with research, and everyone knew its value. Nowadays, it is widely used in experiments and production, and is indispensable to chemistry, biology, and other industries. It has made great contributions to the path of science and evolution, and has also witnessed the unremitting exploration of the academic community.

Product Overview

Today there is a product called 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer. This agent is an important substance for chemical research. Its properties are stable, and in a specific reaction system, it can maintain the balance of pH and ensure a smooth reaction.
Looking at its quality, it is in the shape of powder, pure and uniform in color, and has no variegated odor. It is easy to dissolve in water, quickly soluble and clear in liquid, and has no appearance of precipitation and flocculation. It is useful in various fields such as biochemistry and medicine.
In biochemical experiments, when cell culture and enzymatic reactions are carried out, it is necessary to stabilize the pH value, and this agent is worthy of the important task. In pharmaceutical research and development, the development of preparations and pharmacological research also depend on the environment of retinoic acid and alkali. In conclusion, this 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer is essential in the field of chemical research.

Physical & Chemical Properties

4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer, in the field of chemistry, its physical and chemical properties are crucial. The appearance of this agent is often white crystalline powder, with uniform and delicate texture. Looking at its solubility, it is easily soluble in water, and quickly disperses and melts in water, just like salt into soup, invisible but effective.
When it comes to stability, under normal environmental conditions, it is quite stable. However, when encountering strong acids and alkalis, it may react, such as a boat sailing in a sea of clouds and clouds. Its pH buffer range is specific, just like the balance of accurate checks and balances, maintaining the pH of the system within a certain range, escorting many chemical reactions and biological processes, ensuring that the environment is suitable, everything goes smoothly, and it will not be disrupted due to changes in pH.

Technical Specifications & Labeling

4- (2-hydroxyethyl) piperazine-1 -ethanesulfonate sodium salt buffer, its technical specifications and identification (product parameters) are the key. Looking at this product, it is necessary to clarify its properties, the color should be pure and uniform, and there should be no variegated foreign matter. The standard of its purity, when it is very high, the impurity content must be fine. The pH buffer range is the essence of this product, and it needs to be accurate in a specific range to meet the needs of various experiments. And good solubility, instant and uniform in water or specific solvents. On the label, the product name is clear, and the parameters such as composition, purity, pH range, and storage conditions are detailed, so that the user can see it at a glance, without the risk of misuse, so as to ensure accurate experiments, smooth operation, and correct technical specifications and labels. This product is a good product for use.

Preparation Method

To prepare 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer, the raw materials and production process, reaction steps and catalytic mechanism are the key. Prepare an appropriate amount of 2-hydroxyethylpiperazine and ethanesulfonate in a precise ratio. In a suitable temperature reaction kettle, a specific catalyst is added to promote the reaction between the two. Control its temperature, pressure and reaction time to make the reaction progress steadily. After the reaction is completed, the impurities are removed through purification and refining to obtain a pure product. This preparation method requires following the procedures and fine operation to obtain high-quality 4- (2-hydroxyethyl) piperazine-1-ethane sulfonate sodium salt buffer products for experimental or production needs.

Chemical Reactions & Modifications

Fu 4- (2 -hydroxyethyl) piperazine-1 -ethanesulfonate sodium salt buffer is very important in the field of chemistry, related to reaction and modification. Its properties can stabilize the reaction environment, and the transformation should proceed in an orderly manner. To improve its properties, researchers often study the causes.
Looking at the reaction, the temperature and concentration of the medium can be controlled. When the temperature rises, the molecular energy increases, and it should be accelerated or increased; when the concentration changes, the particle collision frequency changes, and it also involves the direction and rate of response.
As for modification, or adding additives, or adjusting the structure. Additives enter, or promote, or suppress side reactions; structural changes, or are easily soluble, or stabilize.
With the progress of research, this buffer has become better and better, and it has become more and more effective in the fields of biochemistry and medicine. It is used by researchers to promote the progress of chemistry and benefit the prosperity of people's lives.

Synonyms & Product Names

4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer, this substance has a wide range of uses in biochemical experiments. Its aliases are many and well known in the industry.
The field of Guanfu biochemistry, buffers are essential. This 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer can maintain the acid-base balance of the system and maintain the stability of biochemical reactions.
It has many aliases, such as the name of so-and-so, all refer to this substance. In the laboratory, it is often used by researchers in cell culture, protein research, etc., and has significant effect. It can precisely regulate the pH of the reaction environment, help the experiment to advance smoothly, and obtain accurate results. It is an indispensable product for biochemical research.

Safety & Operational Standards

4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer
Fu4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer is widely used in chemical experiments and various biomedical research. To ensure that the experiment is smooth and safe, it is necessary to specify its safety and operation specifications.
In terms of safety, although this buffer is usually stable in nature, it should be cautious to avoid direct contact. If you accidentally touch the skin, rinse it with plenty of water as soon as possible. If you feel uncomfortable, you should seek medical attention immediately. If you accidentally touch your eyes, you need to rinse them with flowing water for several moments immediately, and then seek medical treatment. Do not neglect it. As for inhalation or accidental ingestion, it will also endanger your health. Therefore, when operating, you must do it in a well-ventilated manner to prevent inhalation of dust or mist.
When talking about the operating specifications, first and foremost, when preparing, measure the components of the buffer according to the precise proportion. When weighing the medicine, you must use a precise weighing instrument, and the operation should be careful to avoid errors. During the dissolution process, you can stir it moderately to promote its instant dissolution, but the force of stirring should not be too violent to avoid the solution splashing out. After the preparation, you need to use a precise instrument to measure its pH value to ensure that it meets the needs of the experiment.
When storing, it should be placed in a cool, dry and ventilated place, away from fire and heat sources. At the same time, it must be properly sealed to prevent moisture deterioration or reaction with other objects. When using, the utensils used must be clean and dry to prevent the introduction of impurities, which will affect the quality of the buffer and the experimental results.
In short, when using 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer, strict safety and operating practices can ensure the accuracy of the experiment and the safety of personnel, so that the research can be carried out smoothly.

Application Area

Taste the wonders of pharmaceuticals, it is related to all things. Today there are 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffers, which are widely used. In the field of biochemical experiments, it can stabilize the acid and base of the system and make the reaction smooth. Such as the catalysis of enzymes, it needs to be adapted to develop its ability, and this buffer can be used. The genus of cell culture also depends on it to maintain the stability of the internal environment, maintain the safety of cells, and make it multiply and orderly. In medical testing, it helps to accurately measure and analyze, and is the basis for diagnosis and treatment. In chemical synthesis, it regulates the reaction conditions and promotes the formation of products. This agent is very useful in many ways, and it is an indispensable material.

Research & Development

In recent years, Yu dedicated himself to the research of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt buffer. This agent is widely used in biochemical experiments, and the quality of its performance is related to the success or failure of the experiment.
Yu Chu observed its properties, analyzed its physicochemical properties in detail, and explored its stability in different environments. After repeated experiments, the buffer range and pH value control were studied, and efforts were made to be accurate. During this period, he encountered many problems, such as the influence of impurities and the difficulty of preservation.
However, he was not discouraged, and read all the books and learned from all the sages. In the end, there is a method to optimize the preparation, increase its purity, and improve its preservation. Nowadays, the performance of this agent is gradually improving and the application is wider. Follow-up research in the remaining period can make it more perfect and help more in the biochemical field.

Toxicity Research

Taste of the way of chemistry, related to the nature of all things, in the study of pharmaceuticals, especially its toxicity. Today there are 4- (2 -Hydroxyethyl) Piperazine -1 -Ethanesulfonic Acid Sodium Salt Buffer, my generation studied it.
Examine this agent in detail, to understand its toxicity. Many experiments, observe its response to things, observe its changes in the body. Although it has not achieved all the results, it has begun to be obtained. In common things, the reaction is still slow, and it seems that there is no urgent poison. However, it should not be taken lightly, and the study of toxicity cannot be completed for a while.
It still needs to be deeply cultivated, and it needs to be carefully explored with the care of ancient methods. Or observe its long-term effect, or test its different amounts should be. Hope to be able to fully understand its properties, so that this medicine, for medical use, research, can be safe and good, without fear of its poison, to help the world.

Future Prospects

The prospect of the future is very important for the buffer of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonate sodium salt. Looking at this agent today, it has made its mark in the field of biochemical experiments, but the technology is new, and there should be more refinements in the future. It may be possible to optimize the synthesis method, reduce its cost, increase its yield, and make it available to more researchers. It is also expected to expand its scope of application, and play a greater role in new reaction systems and special biological environments. In this way, it can meet the diverse needs of future scientific research, help the scientific boat, break through the waves, and sail into the unknown and bright territory.

Frequently Asked Questions

What are the main application fields of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, sodium salt buffer?

The main application field of 4 - (2 -hydroxyethyl) piperazine-1 -ethanesulfonic acid (HEPES) and zinc acetate buffer solution is related to biochemical and cell biology experiments.
Husband HEPES is an excellent amphoteric buffer with many characteristics suitable for biological systems. It can maintain a stable pH over a wide pH range and has little interference with most biochemical reactions. In the field of cell culture, this buffer is often used. Cover cell growth is extremely sensitive to environmental pH. HEPES can ensure that the pH of the cell culture environment is stable, so that cells can grow and proliferate under suitable conditions, and are protected from large fluctuations in pH.
And zinc acetate buffer solution, zinc ions have important physiological functions in organisms and participate in the composition and regulation of many enzymes. In some biochemical experiments, it is necessary to simulate a specific biological environment. Zinc acetate buffer solution comes in handy, providing a stable concentration of zinc ions to facilitate the study of related biochemical reactions, such as some enzymatic reactions that rely on zinc ions.
Furthermore, in protein research, these two are also quite useful. The structure and function of proteins are extremely sensitive to the environment. HEPES and zinc acetate buffer solution can cooperate to maintain a suitable pH and ionic strength, so that the protein maintains its natural conformation and activity, which is convenient for protein purification, crystallization and activity analysis experiments.
In summary, 4 - (2-hydroxyethyl) piperazine-1 - ethanesulfonic acid and zinc acetate buffer solution play an indispensable role in the experimental research of biochemistry and cell biology, in order to create a stable and suitable reaction environment, helping researchers to deeply explore the mystery of life.

4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, sodium salt buffer, what are the precautions when storing?

Fu 4 - (2 - hydroxyethyl) piperazine - 1 - ethanesulfonic acid (HEPES) and calcium chloride buffer should be paid attention to when storing.
First, the temperature is very important. This buffer should be stored in a low temperature place, but it should not be frozen. If the cap temperature is too high, or the chemical reaction of the components in the buffer will be intensified, which will affect its buffer performance. If the temperature is too low to freeze, the structure of the buffer may be damaged, and it will be difficult to restore its original properties after thawing.
For the second time, light should not be ignored. When avoiding direct exposure to strong light, it should be placed in a dark place. The photochemical reaction of some components in the buffer due to light or causes it to deteriorate, thereby impairing the stability and effectiveness of the buffer.
Furthermore, sealing is the key. Be sure to ensure that the container is well sealed to prevent impurities such as carbon dioxide and water vapor in the air from mixing. The dissolution of carbon dioxide into the buffer can change its pH; the mixing of water vapor or the change of the concentration of the buffer is not conducive to the quality of the buffer.
In addition, the buffer should be checked regularly during storage. Check whether it has precipitate formation, color change or odor. If precipitation is seen, or a reaction occurs between components; changes in color and odor also suggest that the buffer may have deteriorated. Once such abnormalities are detected, they cannot be used again, so as not to affect the experimental results.
In conclusion, in order to maintain good performance of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid and calcium chloride buffer during storage, careful attention must be paid to temperature, light, sealing and regular inspection to ensure its quality for experimental needs.

What is the pH range of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, sodium salt buffer?

The observer's question is about the pH range of (2-hydroxyethyl) piperazine-1-ethanesulfonic acid and borax buffer. These two have a wide range of uses in biochemical experiments, and knowing their pH range is the key to the success of the experiment.
(2-hydroxyethyl) piperazine-1-ethanesulfonic acid, often referred to as HEPES, is a zwitterion buffer. The pH range of HEPES is about 6.8 to 8.2. Within this range, its buffering ability is quite strong, which can effectively maintain the stability of pH solution. Because HEPES has a special chemical structure, it can adjust the pH value of the solution through the gain and loss of its own protons in an acidic or alkaline environment. In cell culture, protein crystallization and other experiments, HEPES is often the first choice buffer, because it can provide biomacromolecules with pH conditions similar to the physiological environment, so that the structure and function of biomolecules can be maintained stable.
Borax buffer is mainly composed of borax (sodium tetraborate). The pH range of borax buffer is about 8.0 to 10.0. Borax will undergo hydrolysis in aqueous solution to produce boric acid and borate ions, which form a buffer pair. When the pH of the solution changes, boric acid and borate ions can resist the change of pH through the transfer of protons. In some experiments that require alkaline environments, such as some enzyme activity measurement experiments, borax buffer can create suitable alkaline conditions to ensure enzyme activity.
In summary, the pH range of (2-hydroxyethyl) piperazine-1-ethanesulfonic acid is about 6.8-8.2, and the pH range of borax buffer is about 8.0-10.0. The experimenter should carefully choose the buffer according to the specific needs of the experiment to achieve the expected effect of the experiment.

4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, sodium salt buffer with which substances may react

In the genus of borax, its properties are unique, and there are many people who can be investigated in the response of various things. Borax, with its cool and slippery nature, has long been recorded in medical herbs.
In this word, borax buffer, this liquid changes when it encounters acid and alkali. In case of strong acid, the hydrolysis equilibrium of borax shifts, and the weak acid ion in borax combines with the hydrogen ion of the strong acid to form a weak acid. In case of hydrochloric acid, the hydrogen ionization of borate and hydrochloric acid in borax synthesizes boric acid. It should also be like yin and yang, blending with each other, and each has its own place.
When encountering a strong base, the boric acid produced by borax neutralizes with the strong base. Take sodium hydroxide as an example, boric acid and sodium hydroxide contact to form borate and water. This should also be, such as ice charcoal, and return to peace.
It is also interesting to look at its relationship with metal salts. Some metal ions can be complexed with borate ions generated by borax to form complexes with specific structures and properties. Such as with copper ions, blue complexes can be produced, which can be used as evidence for identification. The beauty of its application is like a natural work of art, which seizes good luck.
As for organic matter, borax buffer can also respond to it. In some organic reactions, borax buffer can be used as a medium to stabilize the environment and promote the progress of the reaction. For example, in specific organic synthesis, it maintains the stability of the pH of the reaction system, so that the reaction proceeds in sequence without disorder.
The response of borax buffer to various things, whether it is in color or in quality, all follow the laws of nature.

How to choose the optimal concentration of 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid, sodium salt buffer in different experiments

In order to select the best concentration of 4 - (2-hydroxyethyl) piperazine-1-ethanesulfonic acid and calcium chloride buffer in different experiments, it is necessary to clearly investigate the characteristics and requirements of various experiments.
In biochemical experiments, the activity of many enzymes is highly susceptible to the influence of environmental pH and ionic strength. If studying enzymatic reactions, 4 - (2-hydroxyethyl) piperazine-1-ethanesulfonic acid buffer is often the first choice because it can maintain a stable pH environment. Generally speaking, its concentration should be controlled between 10-100 mM. When the concentration is too low, it may be difficult to effectively resist external acid and base changes, causing pH fluctuations and affecting enzyme activity; if the concentration is too high, it may cause spatial resistance to enzyme molecules, or change the ionic strength of the solution, which also interferes with enzymatic reactions.
Calcium chloride buffer is crucial in reactions involving calcium ions, such as some cell signaling pathways. The optimal concentration varies according to the specific reaction, and the common range is 1-10 mM. If the concentration is too low, the calcium ion effect is difficult to show; if the concentration is too high, it may cause non-specific binding and disrupt the normal reaction process.
In cell culture experiments, 4- (2-hydroxyethyl) piperazine-1-ethanesulfonic acid buffer can assist in maintaining the pH stability of the culture medium and ensure that cells grow in a suitable environment. Usually the concentration is set at 5-25 mM. Improper concentration may cause acid-base imbalance in the microenvironment of the cell, hinder cell growth and even cause apoptosis. In such experiments, calcium chloride is related to physiological processes such as cell adhesion and proliferation, and the optimal concentration is usually between 0.5-2 mM. Concentration deviation may affect the cytoskeleton structure or interfere with cell-to-cell communication.
Overall, in order to obtain the best concentration of the two, it is necessary to carefully consider and carefully explore according to the experimental purpose, sample characteristics and other factors. After many pre-experiments and optimizations, accurate and appropriate concentrations can be obtained to help the experiment go smoothly and obtain reliable results.