4-Morpholinoethanesulfonic Acid

4-Morpholinoethanesulfonic Acid, Chinese name 4-morpholine ethanesulfonic acid, often referred to as MES. Its main uses are many, let me tell you one by one.
In the field of biochemistry and molecular biology experiments, MES plays a significant role. Because it has good buffering properties and can maintain a specific pH range, it is crucial in many biochemical reactions. Many enzymatic reactions are extremely sensitive to environmental pH, and MES buffers can create a stable environment for enzymes to maintain activity and function. For example, in protein separation and purification experiments, MES buffers can be prepared to a suitable pH to optimize the charge state of proteins and improve the separation effect and purity.
MES is also indispensable in cell culture. Cell growth is demanding on the environment, and pH stability is a key factor. The MES buffer system helps maintain the pH stability of the cell culture medium, providing a suitable microenvironment for cell growth and proliferation. Different cell lines have different preferences for pH. MES buffers can be precisely adjusted according to specific needs to ensure normal physiological activities of cells and reduce cell damage or death due to pH fluctuations.
In addition, MES is widely used in the field of drug development. During drug synthesis, specific reaction conditions need to be suitable for the pH environment, and MES buffers can be provided on demand. In drug quality studies, the physiological environment is simulated to evaluate the stability and solubility of drugs, and the MES buffer simulates the pH of body fluids, which helps to accurately judge drug performance and provides a key reference for drug dosage form design and optimization.
Furthermore, in electrophoresis experiments, MES is used as a buffer to adjust the pH of the electrophoresis system to ensure the orderly migration of biological macromolecules such as proteins and nucleic acids in the electric field according to their own charge and size, and to achieve efficient separation and analysis.
In summary, 4-Morpholinoethanesulfonic Acid has important uses in biology, medicine and other fields, and is of great significance to scientific research progress and practical applications.

4-Morpholinoethanesulfonic Acid, or 4-morpholine ethanesulfonic acid, is often referred to as MES. This is a buffer commonly used in biochemical research. Its physicochemical properties are as follows:
MES has a white crystalline powder in appearance, stable properties, and easy to store and use. It is highly soluble in water, which allows it to quickly and uniformly disperse in the solvent when configuring the buffer solution to form a uniform and stable system. However, it has low solubility in common organic solvents such as ethanol and acetone, which should be paid attention to when experimenting with solvents.
From the perspective of acid-base characteristics, the pKa value of MES is about 6.1 (25 ° C), which means that when the pH value is close to 6.1, it exhibits excellent buffering ability, which can effectively maintain the stability of the pH value of the solution and prevent large fluctuations in the pH value due to external factors. Many physiological processes in organisms can proceed normally within a specific pH range, and the MES buffer solution can provide a stable acid-base environment for related reactions in this scenario.
In terms of thermal stability, MES is quite stable under normal experimental temperature conditions, but when the temperature is too high, chemical changes such as decomposition may also occur, which in turn affects its buffering performance. Therefore, it is necessary to control the appropriate temperature during storage and use to avoid adverse effects of high temperature.
In addition, the chemical properties of MES are relatively stable, and it is not easy to chemically react with common metal ions, reducing agents, oxidizing agents, etc. under normal conditions, which makes it possible to coexist with a variety of biomolecules or chemical reagents in the same system without interfering with each other, which is of great significance in the construction of complex biochemical experimental systems.

4 - Morpholinoethanesulfonic Acid (MES) is a commonly used buffer in biochemical experiments. When storing this material, all points must be paid attention to.
The first to bear the brunt, temperature is of paramount importance. MES should be stored in a low temperature and stable place, usually refrigerated at 2 - 8 ° C. This is due to high temperature, which can easily cause its chemical properties to change, accelerate decomposition or deterioration, and then affect its buffering performance. If the ambient temperature is too high, the MES structure may be damaged, the buffering effect will be greatly reduced, and the experimental results will be difficult to be accurate.
Secondly, the control of humidity should not be underestimated. Humid environment is prone to moisture in MES, which will not only change its physical form, such as agglomeration, but also may trigger chemical reactions and reduce purity. Therefore, the storage place must be dry, and a desiccant can be prepared to maintain the dry environment.
In addition, light will also affect MES. It should be stored in a dark place or in a brown bottle. Light may induce luminescent chemical reactions, change the molecular structure of MES, and cause its performance to change.
In addition, it is necessary to pay attention to the choice of storage containers. Corrosion-resistant and well-sealed containers should be used to prevent MES from reacting with the material of the container, and good sealing can avoid contact with air, prevent oxidation and absorb impurities in the air.
In short, proper storage of MES, strict control of temperature, humidity, light, and selection of suitable containers can ensure its stability, play a proper buffering role in the experiment, and ensure the reliability and accuracy of the experimental results.

4 - Morpholinoethanesulfonic Acid, also known as 4 - morpholine ethanesulfonic acid, often abbreviated as MES, is a commonly used buffer in biochemical research. During its use, safety precautions are quite important, as detailed below:
First protective measures. MES has a certain degree of irritation and requires comprehensive protection during contact. Be sure to wear suitable protective gloves when operating. Materials such as nitrile rubber gloves can effectively block its contact with the skin and avoid skin irritation causing redness, swelling, itching and other discomforts. At the same time, protective glasses are also indispensable to prevent MES powder or solution from splashing into the eyes, damaging eye tissue, causing pain, tears and even vision impairment. If it is a poorly ventilated place, a mask should also be worn to prevent inhalation of MES powder and avoid irritation of the respiratory tract, causing symptoms such as cough and asthma.
The second is the storage requirement. MES should be stored in a cool, dry and well-ventilated place. Excessive temperature can easily cause changes in its chemical properties and affect the use effect. Humid environment may make MES absorb moisture, which not only affects the purity, but also may cause caking and other conditions. It is also crucial to store it separately from oxidants, acids, alkalis and other substances, because it may chemically react with these substances, causing fire, explosion and other hazards.
Re-discussion of the specifications for use. When configuring MES buffer, be sure to follow the exact proportions and steps. Weighing should be accurate, otherwise the pH value of the buffer and other properties do not match expectations and affect the accuracy of the experimental results. The dissolution process needs to be fully stirred to ensure that the MES is completely dissolved and reaches a uniform and stable state. During use, closely monitor the pH value change of the reaction system. The MES buffer capacity is limited. If the system is too acidic or alkaline, it may exceed the buffer range and cause pH to go out of control.
Talk about emergency treatment at the end. If you accidentally come into contact with the skin, you should immediately rinse with a large amount of flowing water. The rinsing time should be 15 to 20 minutes to remove the MES from the skin as much as possible. If there is still discomfort after rinsing, you need to seek medical attention in time. If it splashes into the eyes, rinse with a large amount of water quickly. When rinsing, turn the If the inhalation of MES powder, quickly transferred to fresh air, keep the respiratory tract unobstructed, if breathing difficulties and other serious symptoms, immediately call first aid.

4 - Morpholinoethanesulfonic Acid (abbreviated as MES) is a buffer often used in biochemical and molecular biology experiments. Whether it will react with other chemical substances depends on the specific type of substance it is exposed to.
If a strong acid and a strong base are encountered, MES will react with it to regulate the pH of the solution because of its buffering properties. For example, when a strong acid is added to a buffer system containing MES, MES will bind hydrogen ions to maintain pH stability; when a strong base is added, it will release hydrogen ions to neutralize.
As far as metal ions are concerned, MES may be able to complex with some metal ions by virtue of the coordination atoms such as nitrogen and oxygen it contains. Such as transition metal ions such as copper ions and zinc ions, under suitable conditions, or form stable complexes, which may change the chemical properties and activities of metal ions.
When MES coexists with some organic compounds with active functional groups, the reaction depends on the characteristics of functional groups. Like organic compounds containing active halogen atoms, carboxyl groups, amino groups and other functional groups, under specific reaction conditions, such as suitable temperature, catalyst presence, or with ethanesulfonic acid groups or morpholine groups of MES Substitution, condensation and other reactions occur.
However, if other substances are chemically stable, such as inert gases, certain saturated alkanes, etc., under normal conditions, MES is difficult to react with. Overall, whether MES can react with other chemical substances requires comprehensive consideration of various factors such as the chemical properties of the substance and the reaction conditions in order to accurately determine.