Bes( N,N-Bis-2-Hydroxyethyl)-2-Aminomethanesulfonic Acid

Bes (N, N-bis-2-hydroxyethyl) -2-aminomethanesulfonic acid, which is widely used in many fields such as biochemical experiments and drug development.
In the context of biochemical experiments, it is often used as a buffer. Biochemical reactions often need to occur smoothly in a specific pH environment, and the pH changes slightly, or the reaction rate and protein activity are disturbed. Bes buffers can stabilize and control the pH of the reaction system and keep it within a predetermined range. For example, when protein is purified and enzyme activity is measured, many proteins and enzymes are only active in a specific pH range. By using Bes buffers to create an appropriate environment, the experiment can be accurate and reliable.
In the field of drug research and development, it also plays a key role. In the process of drug development, it is necessary to simulate the human physiological environment to measure the properties and effects of drugs. The pH of the human physiological environment is relatively constant, and Bes buffer can simulate this environment, which can help researchers observe the stability, solubility and interaction with biomolecules of drugs, providing an important basis for the evaluation of drug resistance.
The field of cell culture is also indispensable. When cells are cultured in vitro, they are extremely sensitive to environmental pH. Appropriate pH is essential for normal cell growth and metabolism. Bes buffer can maintain the pH stability of the culture medium, prevent pH fluctuations caused by the accumulation of cell metabolites, and protect cells from growing and proliferating in a good environment. It is of great significance for cell biology research and biological product production.
In the field of analytical chemistry, Bes is also useful. In some analytical methods, such as high performance liquid chromatography, the pH of the mobile phase has a great influence on the separation effect. Bes buffer can adjust the pH of the mobile phase, optimize the separation conditions, and improve the sensitivity and accuracy of analysis.
In short, Bes (N, N-bis-2-hydroxyethyl) -2-aminomethanesulfonic acid is an important reagent in the fields of biochemistry, medicine, cell culture and analytical chemistry due to its unique properties, providing key support for scientific research and production.

Bes (N, N -bis-2-hydroxyethyl-2-aminomethanesulfonic acid) is a commonly used zwitterion buffer in biochemical research, and its physicochemical properties are unique.
First word solubility, Bes is easily soluble in water, can be rapidly dispersed in water and achieve a uniform solution state, this characteristic makes it easy to integrate into various aqueous experimental systems, laying a good foundation for subsequent reactions or tests.
times and stability. Under conventional experimental conditions, Bes has high chemical stability, can resist the interference of many chemical reactions, and is not easy to decompose by itself or react with common chemical substances. In this way, it ensures that the buffer system maintains a stable pH environment during the experimental process, providing a guarantee for the reliability of the experimental results.
Furthermore, the buffering performance is the key. Bes has a specific pKa value and can exhibit excellent buffering ability within a specific pH range. It can effectively resist the pH change caused by the addition of a small amount of acid or alkali, so that the pH value of the solution is maintained at a relatively stable range, thus creating a suitable environment for biochemical reactions that depend on specific pH conditions.
In addition, Bes has a clear melting point, and this physical parameter can be used to identify its purity. The melting point of high-purity Bes is more accurate and fixed. If the purity of the sample is not good, the melting point will often shift or the melting range will widen.
Finally, in terms of appearance, Bes usually appears as a white crystalline powder, which is convenient for observation and accurate weighing, which is conducive to the accuracy of experimental operation.
In summary, Bes is widely used in the field of biochemical experiments due to the above physical and chemical properties, creating stable and suitable conditions for many biochemical reactions and analytical tests.

Bes (N, N -bis-2-hydroxyethyl-2-aminomethanesulfonic acid) is a commonly used zwitterionic buffer in biochemical research. Its differences from other similar compounds can be summarized as follows:
Preliminary structure, Bes has a unique structure, the molecule contains two hydroxyethyl groups and one aminomethanesulfonic acid group. This specific structure gives it specific physicochemical properties. Compared with other buffers, or due to the position and number of hydroxyethyl groups, or the state of aminomethanesulfonic acid groups. If a similar buffer has less one hydroxyethyl group, its hydrophilicity is not as good as Bes, and Bes is more suitable for high hydrophilic systems.
Second, the buffer performance of Bes is about pH6.4-7.8, which is the key to its use in a specific acid-base environment. Different similar compounds have their own buffer ranges. If the buffer range of a buffer is at pH5-6, it is more suitable for the strong acid; while Bes is better in the near-neutral range. When the biological system wants to maintain a near-neutral pH, Bes can be stabilized and controlled, but other buffers are not competent.
Talking about solubility again, Bes has excellent water solubility and can form a homogeneous solution faster than water, which is convenient for biochemical experimental operation. However, other similars may have poor water solubility due to their structure, and they will be in trouble when formulating solutions. It is necessary to assist in the solution and increase the experimental complexity.
Also known as chemical stability, Bes is stable under normal conditions and is not easily affected by oxidation, hydrolysis, etc. However, some similar compounds, or because they contain volatile groups, require special care during storage and use, and are prone to deterioration, which affects the accuracy and reproducibility of the experimental results.
At the end of biocompatibility, Bes has good biocompatibility and little interference with biological systems. However, some similar compounds may have negative effects on biological molecules such as cells and proteins due to structural factors, which are not suitable for biological experiments.

When storing and transporting Bes (N, N-bis-2-hydroxyethyl) - 2-aminomethanesulfonic acid, many matters need to be paid attention to. This substance should be stored in a cool, dry and well-ventilated place to prevent moisture. Moisture can easily cause its properties to change, affecting its quality and utility.
When handling, be sure to handle it with care and must not be loaded and unloaded brutally. Because if it is subject to violent collision or falling, or the packaging is damaged, the material will be exposed and accidents will be caused. The packaging must be tight to prevent leakage. Once leaked, it will not only cause material loss, but also pose a threat to the environment and human health.
During storage, it should be stored separately from oxidants, acids, alkalis and other substances, and must not be mixed. This is determined by its chemical properties. Contact with these substances is prone to chemical reactions and even causes danger. At the same time, the stored Bes should be checked regularly to see if the packaging is damaged and whether the material has deteriorated, so as to detect and deal with problems in time.
During transportation, the transportation vehicle should be equipped with corresponding protective equipment, such as rain protection, sun protection facilities, etc., to ensure that the transportation environment is suitable. And the transportation personnel should be familiar with the characteristics of the substance and emergency treatment methods. If an unexpected situation occurs on the way, they can respond quickly and properly to avoid serious consequences.

The method for preparing Bes (N, N -bis-2-hydroxyethyl-2-aminomethanesulfonic acid) follows the following path.
First, 2-aminoethanesulfonic acid and ethylene oxide are used as starting materials. First, 2-aminoethanesulfonic acid is placed in a suitable reactor, and an appropriate amount of solvent, such as alcohol solvent methanol or ethanol, is added to create a homogeneous reaction environment. Warm up to a moderate temperature, usually at 40-60 ° C, and slowly introduce ethylene oxide gas. This reaction needs to be carried out under stirring to make the material fully contacted. Ethylene oxide reacts with 2-aminoethanesulfonic acid in a molar ratio of 1:2. After the nucleophilic substitution process, the two hydrogen atoms on the amino group are gradually replaced by 2-hydroxyethyl to form Bes. The reaction process is monitored by thin-layer chromatography. When the raw material point disappears, it indicates the end of the reaction. After that, the solvent is removed by reduced pressure distillation, and the residue is purified by recrystallization to obtain a pure Bes product.
Second, use 2-chloroethanesulfonic acid and diethanolamine as raw materials. In the reaction vessel, mix diethanolamine and 2-chloroethanesulfonic acid in a molar ratio of 1:1.2, and add an appropriate amount of basic catalyst, such as sodium hydroxide or potassium carbonate. Select a polar aprotic solvent, such as N, N-dimethylformamide (DMF), and heat the reaction at 60-80 ° C. The amino nucleophilic group of diethanolamine attacks the chlorine atom of 2-chloroethanesulfonic acid, and undergoes a substitution reaction to form the target product Bes. After the reaction is completed, the reaction liquid is cooled, poured into a large amount of water, and the pH is adjusted to neutral with dilute acid. The precipitated solid is filtered, washed with water, and dried to obtain the Bes finished product.
The above preparation methods have their own advantages and disadvantages, and need to be carefully selected according to actual needs and conditions.