What is the chemical structure of Bes N, N-Bis (Hydroxyethyl) -2-Aminoethanesulfonic Acid?
Bes (N - Bis (Hydroxyethyl) - 2 - Aminoethanesulfonic Acid), its chemical structure is as follows:
The molecular backbone of this substance is ethylamine structure, and a hydrogen atom on the amino group of ethylamine (\ (- NH_2\)) is replaced by a group containing two hydroxyethyl groups (\ (- CH_2CH_2OH\)), that is, the formation of\ (N -\) bis (hydroxyethyl) amino moiety; at the same time, a carbon atom of ethylamine is connected to a sulfonic acid group (\ (- SO_3H\)).
From the structural analysis, it has multiple polar groups. The hydroxyl group in the two hydroxyethyl groups (\ (-OH\)) makes the molecule have good hydrophilicity, which can form hydrogen bonds with water molecules and increase its solubility in water. The sulfonic acid group (\ (- SO_3H\)) can be partially ionized in solution, giving the molecule certain acidic and ionic characteristics. This characteristic is of great significance in application scenarios such as buffer solutions. The ethylamine skeleton acts as a bridge connecting various functional groups to maintain the overall structural stability of the molecule.
This structure determines that Bes is often used as a buffer in biochemistry and molecular biology experiments. By virtue of the polar groups in its structure, it can effectively resist the pH change of the solution and maintain the relative stability of the pH value of the system, thus providing a suitable environment for biochemical reactions.
Bes N, N-Bis (Hydroxyethyl) -2-Aminoethanesulfonic Acid is commonly used in which experiments
Bes, that is, N - Bis (Hydroxyethyl) - 2 - Aminoethanesulfonic Acid, Chinese name N, N - bis (2 - hydroxyethyl) - 2 - aminoethanesulfonic acid, this substance is commonly used in biochemical, molecular biology and other experiments.
In protein research experiments, its use is quite extensive. Protein properties are easily affected by the environment, and pH value is particularly critical. Bes has good buffering properties, which can create a stable pH environment, so that proteins maintain their natural conformation and activity. For example, in protein crystallization experiments, by precisely controlling the pH value to the optimal protein crystallization range, Bes can play a buffering effect and help proteins to form crystals in an orderly arrangement, which is convenient for subsequent structure analysis.
Bes is also common in cell culture experiments. Cell growth requires strict environmental requirements, and a suitable pH value is indispensable. Adding Bes to the cell culture solution can effectively maintain the pH stability of the culture solution and create a suitable microenvironment for cell growth. Whether it is adherent cells or suspended cell culture, a stable pH environment helps cells to metabolize and proliferate normally, and reduces cell damage and death caused by pH fluctuations.
Enzyme activity research experiments also require the help of Bes. Enzymatic reactions are extremely sensitive to pH, and different enzymes have their optimum pH values. The Bes buffer system can provide a specific pH environment according to experimental needs, allowing enzymes to exhibit the best activity, which is convenient for researchers to accurately determine enzyme kinetic parameters and explore enzyme action mechanisms. For example, the catalytic efficiency and substrate affinity of some key enzymes involved in metabolic pathways can be accurately determined under the stable pH condition maintained by Bes.
What is the buffer range of Bes N, N-Bis (Hydroxyethyl) -2-Aminoethanesulfonic Acid?
Bes, or N, N-bis (hydroxyethyl) -2-aminoethanesulfonic acid, is a commonly used buffer in biochemistry and molecular biology experiments. Its buffer range is about pH 6.4 - 7.8.
The reason why this buffer has good buffering ability in this pH range is due to its own chemical structure. The amino and hydroxyl groups contained in its molecular structure can react with hydrogen ions or hydroxyl ions in the solution under specific pH conditions, thereby achieving the purpose of stabilizing the pH value of the solution.
When the pH value of the solution tends to be acidic and the concentration of hydrogen ions increases, the amino groups in the Bes molecule will bind hydrogen ions and slow down the decrease in pH value; if the pH value of the solution tends to be alkaline and the concentration of hydroxide ions increases, the hydroxyl groups in the Bes molecule will release hydrogen ions and neutralize hydroxide ions, inhibiting the rise in pH value.
In many biological experiments, such as protein purification, enzyme activity determination, etc., pH value needs to be precisely controlled to ensure that biological macromolecules such as proteins and enzymes maintain normal structure and function. Bes is often selected because its buffer range fits the pH of the environment in most organisms.
In this way, within a suitable pH buffer range, Bes can create a relatively stable chemical environment, help the experiment to proceed smoothly, and ensure the accuracy and reliability of the experimental results.
How is the solubility of Bes N, N-Bis (Hydroxyethyl) -2-Aminoethanesulfonic Acid?
This substance is N, N-bis (hydroxyethyl) -2-aminoethanesulfonic acid, which is often used as a biological buffer. It has good solubility, is easily soluble in water, and can show good solubility characteristics in the aqueous phase, providing a stable environment for many biological experiments and reactions.
Because its molecular structure contains hydrophilic groups, such as hydroxyl groups and sulfonic acid groups, hydroxyl groups can form hydrogen bonds with water molecules, and sulfonic acid groups enhance the ionization and dispersion ability of molecules in water, so it has high solubility in water. Under conventional experimental conditions, it can quickly dissolve in water to form a uniform and transparent solution, which meets the buffer preparation requirements of most experiments.
However, the solubility of this substance in organic solvents is different. In general, there is a certain solubility in polar organic solvents such as ethanol, but it is lower than in water; in non-polar organic solvents such as benzene and hexane, due to the large difference between molecular polarity and non-polar organic solvents, the interaction force is weak, and the solubility is extremely low or even almost insoluble.
In short, N, N-bis (hydroxyethyl) -2-aminoethanesulfonic acid has good solubility in water, and the solubility in organic solvents varies depending on the solvent polarity. This property is crucial in related experiments and industrial applications, providing a basis for rational selection of solvents and reaction conditions.
What are the storage conditions for Bes N, N-Bis (Hydroxyethyl) -2-Aminoethanesulfonic Acid?
Bes is N, N-bis (hydroxyethyl) -2-aminoethanesulfonic acid, and its storage conditions are very critical. This substance should be stored in a dry, cool and well-ventilated place. Be sure to keep away from fires and heat sources, because it is more sensitive to heat, and high temperature environments are prone to change its properties.
When storing, it is necessary to ensure that the container is completely sealed to prevent moisture or oxidation reactions from contact with air. Humid air may absorb moisture, affecting purity and performance; oxidation reactions may change its chemical structure and reduce the use effect.
At the same time, it should be stored separately from strong oxidants, strong acids, strong bases, etc., and must not be mixed. These substances come into contact with it, or cause violent chemical reactions, which not only cause Bes to fail, but also may pose a safety hazard.
In addition, the storage area should be equipped with suitable containment materials in case of leakage, which can be properly handled in time to avoid causing a wider range of pollution and hazards. In this way, Bes can maintain good quality and stability during storage.