Bes N-Tris-(Hydroxymethyl)Methyl-2-Aminoethanesulfonic Acid

Bes is N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid, N-Tris- (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid is the full name of N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, both of which are commonly used in biochemistry Zwitterion buffers. The following describes its chemical structure in classical Chinese:
This Bes, its structure is based on ethanesulfonic acid, the sulfuric acid is connected to the B terminal, and the nitrogen atom is connected to the dihydroxyethyl group. Looking at its shape, such as the branch extension, ethanesulfonic acid is dry, hydroxyethyl is branched, and the atoms are connected by bonds according to chemical reasons, in an orderly manner. The nitrogen atom has a lone pair of electrons and can bind to the proton, and the sulfonic acid group can dissociate the proton, which is the source of its buffering properties.
As for N-Tris- (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid, it is based on 2-aminoethyl sulfonic acid. The nitrogen atom of the amino group is connected to the trihydroxymethyl group, and the sulfonic acid group is at the end of the ethyl chain. The overall structure is like a tree, the sulfonic acid group is like the root group, and the trihydroxymethyl group connected by the amino group is like a lush branch and leaf, and the atoms are maintained by covalent bonds to construct this stable state. Due to the fact that the structure contains both proton-acceptable groups and proton-giving parts, the pH value can be stabilized in the biochemical system and the suitable reaction environment can be maintained, which is of great significance in biochemical experiments and research.

Bes is N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid, N-Tris- (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid is N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, both of which are commonly used buffers in biochemical and molecular biology experiments. Its main uses are as follows:
First, it is used for biochemical experiments. In the study of biological macromolecules such as proteins and nucleic acids, it can maintain the pH stability of the system. During the separation and purification of proteins, the protein can maintain activity and structural stability under a specific pH environment. The two buffers can precisely regulate the pH, ensure that the natural conformation and function of the protein are not affected, and lay the foundation for subsequent research. For example, in electrophoresis experiments, a suitable pH environment is the key to ensure the smooth separation of biological macromolecules according to their charge and size, and they can create such an environment.
Second, the field of cell culture. When cells are cultured in vitro, they are extremely sensitive to environmental pH. The use of this type of buffer can maintain the pH of the culture medium in the range suitable for cell growth. Generally, animal cells are suitable for pH between 7.2 and 7.4. Buffers can prevent large fluctuations in pH due to factors such as the accumulation of cell metabolites, create a stable chemical environment for cell growth, and help cells to proliferate and differentiate normally.
Third, the enzyme reaction system is indispensable. The catalytic activity of enzymes is closely related to pH, and different enzymes have their optimum pH. When studying the activity and kinetic characteristics of enzymes, using this buffer to construct specific pH conditions makes enzymes in the best catalytic state, and then accurately explore the mechanism of action, catalytic efficiency and other important parameters of enzymes, which is of great significance for in-depth understanding of metabolic pathways in organisms.

Bes is N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, which is used in different fields at different concentrations.
In biochemistry and molecular biology experiments, it is often used in buffer preparation. For example, in protein and nucleic acid research, the concentration is mostly 5-50 mM. This concentration range can effectively maintain the pH stability of the system and avoid the impact of pH fluctuations on the structure and function of biological macromolecules. For enzyme activity measurement experiments, 5-20 mM Bes buffer can ensure that the enzyme is in a suitable pH environment and accurately reflects the enzymatic reaction activity.
In the field of cell culture, the concentration of Bes is usually 10-25 mM. Cells are sensitive to changes in the pH of the growth environment. This concentration range can maintain the pH stability of the culture medium and create a good environment for cell growth and metabolism. For example, in mammalian cell culture, Bes at about 15 mM can ensure normal adherent growth and physiological function of cells.
In some in vitro diagnostic reagents, the concentration of Bes used is adjusted according to specific detection items and methods, roughly 1-20 mM. For example, reagents that detect specific protein concentrations by immune turbidimetry ensure pH stability of the reaction system by optimizing the concentration of Bes, improving detection sensitivity and accuracy.
In summary, the concentration of Bes used in different fields varies greatly, and precise selection and optimization are required according to the specific experimental purpose, reaction system and biological sample characteristics in order to achieve the best experimental effect and application performance.

Bes, that is, N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, is a commonly used amphoteric ion buffer in biochemical research. Its physical and chemical properties are unique, as follows:
Properties and solubility
Under normal temperature and pressure, Bes is mostly in the form of white crystalline powder, which is easy to store and use. It exhibits good solubility in water and can be rapidly and uniformly dispersed to form a uniform solution. However, in organic solvents such as ethanol and acetone, the solubility is quite low. This property makes Bes widely used in biochemical experiments of aqueous phase systems because it can be easily integrated into aqueous reaction environments and is not disturbed by common organic solvents.
pH and Buffering Capacity
The pKa value of Bes is about 7.15, and it can efficiently maintain the stability of solution pH near this pH. In biochemical experiments, the structure and function of many biomacromolecules are extremely sensitive to pH, and small changes may cause changes in their activity. Bes buffer can resist large fluctuations in pH caused by the addition of a small amount of acid or base, creating a suitable and stable acid-base environment for biological reactions. For example, in enzymatic reaction experiments, the Bes buffer system can ensure that the enzyme is in the optimum active pH range, making the experimental results accurate and reliable.
Stability
Bes is chemically stable, and it is not easy to decompose and oxidize on its own under normal conditions. It can be stored for a long time without basic changes in properties. However, when exposed to extreme conditions such as high temperature, strong acid, and strong alkali, its structure may be damaged and its buffering performance will be reduced. Therefore, when storing Bes, it should be placed in a cool and dry place to avoid contact with strong acid and alkali substances.
Toxicity and biocompatibility
Bes has low toxicity and good biocompatibility. This means that it will not cause significant toxicity and interference to cells or biomacromolecules in biological experiments such as cell culture and protein purification, and can ensure that the biological activity and authenticity of the experimental system are not affected. During the cell culture process, Bes buffer can maintain the pH stability of the culture medium without adverse consequences to cell growth and metabolism, ensuring the smooth development of cell experiments.

Compared with other similar reagents, Bes (N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid) does have its unique advantages.
One is the advantage of buffering performance. The buffer range of Bes is suitable for many biochemical and molecular biology experimental scenarios. It can effectively resist the interference of foreign acid and alkali substances and stabilize the pH value of the solution within a specific pH range. Compared with others, its buffering capacity is more powerful and durable, like a solid barrier, which can make the reaction system proceed smoothly in a stable pH environment and ensure the accuracy and reliability of the experimental results.
Second, good biocompatibility. In experiments that require strict biological activity such as cell culture and enzyme reaction, Bes exhibits excellent biocompatibility. It rarely interacts with biomacromolecules and does not have a significant impact on cell growth, metabolism and enzyme activity. On the other hand, some similar reagents may change their structure and function due to binding to biomolecules, resulting in deviations in experimental results.
Third, good chemical stability. Bes is not easily decomposed or deteriorated by factors such as light, temperature, and oxidation under common experimental conditions. This stability makes it more convenient for experimenters to store and use, and there is no need to worry about its rapid failure due to environmental factors, thus ensuring the repeatability and coherence of the experiment.
Fourth, good solubility. Whether in water or a variety of organic solvents, Bes can quickly dissolve to form a uniform solution. This property allows experimenters to flexibly prepare solutions of appropriate concentrations according to different experimental needs, broadening the scope of their experimental applications.
Overall, Bes has become a favored reagent in many experimental scenarios due to its advantages in buffering performance, biocompatibility, chemical stability and solubility.