What is the chemical structure of Capso-Na: 3- (Cyclohexylamine) -2-Hydroxy-1-Propanesulfonic Acid Monosodium Salt?
3 - (cyclohexylamine) - 2 - hydroxy - 1 - propanesulfonate monosodium salt, which is a common zwitterion buffer in biochemical research. Its chemical structure is as follows:
The main chain of the molecule is propanesulfonic acid structure, and the sulfonic acid group ($- SO_ {3 }^{-}$), binds to the sodium cation ($Na ^ {+} $) at position 1, and then forms a monosodium salt. This structure endows the molecule with good water solubility. At position 2, there is a hydroxyl group ($-OH $), which is polar and can participate in the formation of hydrogen bonds, which affects the solubility and chemical reaction activity of the molecule. Position 3 is connected to the cyclohexylamine group. The cyclohexylamine part is composed of a six-membered ring cyclohexyl group and an amino group ($- NH_ {2} $). The amino group can undergo protonation and deprotonation reactions, and plays a key role in the acid-base buffer system. Overall, the molecule can maintain the relative stability of the pH of the solution within a specific pH range due to the simultaneous presence of acidic sulfonic acid groups and basic amino groups. Its unique chemical structure makes it widely used in many biochemical experiments that require precise control of pH, such as enzyme activity determination, protein crystallization, etc.
What are the main applications of Capso-Na: 3- (Cyclohexylamine) -2-Hydroxy-1-Propanesulfonic Acid Monosodium Salt?
3 - (cyclohexylamine) - 2 - hydroxy - 1 - propane sulfonate monosodium salt, that is, Capso - Na, this substance has many applications in biochemical, medical research and other fields.
In the field of biochemical research, it is often used as a buffer. Biochemical reactions are extremely sensitive to environmental pH, and small changes may affect the reaction process and results. Capso - Na can effectively maintain the pH stability of the reaction system by virtue of its specific acid-base characteristics, which is like building a stable "microclimate" for biochemical reactions. Many enzymatic reactions and protein studies rely on this guarantee.
It is also common in the field of pharmaceutical research and development. In the process of drug development, simulating the physiological environment of the human body is a key link. Human body fluids have a specific pH value, and Capso-Na can help create a similar environment, so that drugs can exhibit more realistic properties in the R & D and testing stage, such as drug solubility and stability studies, etc., all need its assistance.
Furthermore, it is also commonly used in the field of cell culture. When cells are cultured in vitro, they have strict pH requirements for the environment they are in. Capso-Na can precisely regulate the pH of the culture medium to create a suitable environment for cell growth and reproduction, just like building a comfortable "residence" for cells to ensure normal physiological functions and activities, which is of great significance for cell biology research and biological product production.
What are the physical properties of Capso-Na: 3- (Cyclohexylamine) -2-Hydroxy-1-Propanesulfonic Acid Monosodium Salt
This is the sodium salt of 3- (cyclohexylamine) -2 -hydroxy-1 -propane sulfonate, which is an important class of biochemical reagents. Its physical properties are crucial and related to many experimental applications.
When it comes to properties, it is often in the state of white crystalline powder, with fine texture and pure appearance. This form is easy to weigh and dissolve, which is conducive to experimental operation. It has good solubility and can dissolve quickly in water to form a uniform and stable solution, which provides convenience for many biochemical reactions that need to be carried out in aqueous solution.
In terms of melting point, it has a specific melting point value, which is of great significance for the identification of its purity. By measuring the melting point, it can be judged whether the purity of the substance is up to standard. If the melting point is accurate, it usually indicates high purity; if the melting point is deviated, it may imply impurities.
In terms of stability, it is relatively stable under conventional conditions and can be properly stored in a dry and cool environment. However, it is necessary to avoid contact with strong oxidants, strong acids and alkalis and other substances to prevent chemical reactions from causing changes in its structure and properties.
Its density is also an important physical parameter. Although it is rarely used directly in general experiments, in some scenarios where the relationship between volume and mass needs to be accurately measured, density data is indispensable and can assist experimenters in accurately calculating the required dosage.
In summary, the physical properties of this substance are essential for its application in the field of biochemistry, and experimenters need to be familiar with these properties in order to use them reasonably and ensure the smooth progress of the experiment.
Capso-Na: 3- (Cyclohexylamine) -2-Hydroxy-1-Propanesulfonic Acid Monosodium Salt
3- (cyclohexylamine) -2-hydroxy-1-propanesulfonate monosodium salt, namely Capso-Na, is a commonly used reagent for biochemical research. Its storage conditions are quite critical, as follows:
First, the temperature should be placed in an environment of 2-8 ° C. Low temperature can effectively slow down the thermal movement of molecules and reduce the rate of chemical reaction between reagents and substances in the environment, thereby maintaining their chemical stability. If the temperature is too high, it may cause some chemical bonds in the reagent to break, triggering decomposition reactions; and if the temperature is too low, it may cause the reagent to freeze, affecting its physical properties and performance.
Second, humidity should not be ignored, and should be stored in a dry place. Because of its certain hygroscopicity, if the ambient humidity is high, it is easy to absorb water and make the reagent deliquescent. Deliquescence will not only change the concentration of the reagent, but also may cause side reactions such as hydrolysis, which will damage the purity and activity of the reagent.
Third, pay attention to avoiding light. Some reagent ingredients are sensitive to light, and photochemical reactions may occur under light, resulting in structural changes and loss of activity. Therefore, it is advisable to store it in a brown bottle or in a dark cabinet to avoid direct light exposure.
Fourth, keep away from strong oxidizing agents and reducing agents when storing. Capso-Na has a specific chemical structure and may be oxidized in contact with strong oxidizing agents; if it encounters strong reducing agents, it may be reduced. Both situations will change its chemical properties and cannot meet the experimental needs. Capso-Na should be properly preserved and the above conditions should be followed in order to ensure its quality and performance and play its due role in biochemical research experiments.
Capso-Na: 3- (Cyclohexylamine) -2-Hydroxy-1-Propanesulfonic Acid Monosodium Salt
3 - (cyclohexylamine) -2 -hydroxy-1 -propanesulfonic acid monosodium salt. Although the synthesis method of this substance has not been detailed in the past books, it can be thought of according to the following methods according to the principles of chemistry.
The raw material containing cyclohexylamine can be taken first to interact with the compound with hydroxyl and sulfonic acid groups. If cyclohexylamine is reacted with a suitable propylene oxide sulfonic acid derivative, with the help of appropriate temperature, pressure and catalyst, the amino group of cyclohexylamine can be ring-opened and added to the epoxy bond of the propylene oxide sulfonic acid derivative to obtain an intermediate containing the target structure.
Then, the intermediate is neutralized, and an appropriate amount of base, such as sodium hydroxide, interacts with the acidic group in the intermediate to form a sodium salt. After refining and separation, 3 - (cyclohexylamine) - 2 - hydroxy - 1 - propane sulfonate monosodium salt can be obtained.
Or another way, cyclohexylamine is reacted with acrylate compounds to obtain an addition product. After sulfonation, hydrolysis, salt formation and other steps, it is also expected to obtain this substance. However, each method needs to carefully consider the reaction conditions, temperature control, pressure control, catalyst selection, and pay attention to the yield and purity of each step of the reaction. After careful care, you can get a good product.