Capso, Sodium Salt 3-(Cyclohexylamino)-2-Hydroxy-1-Propanesulfonic Acid, Sodium Salt

3- (cyclohexylamino) -2-hydroxy-1-propane sulfonate sodium salt, often referred to as its English abbreviation CAPSO-Na. This substance has a wide range of uses and is a key reagent in the field of biochemical research.
In the study of proteins and enzymes, its role cannot be ignored. The structure and function of proteins and enzymes are highly susceptible to environmental pH, and CAPSO-Na can create and maintain a specific pH environment to ensure that proteins and enzymes are in a stable and active state. For example, in some enzymatic reaction experiments, by precisely adjusting the buffer system containing CAPSO-Na, the enzyme can exhibit the best catalytic activity, which helps researchers to further explore the mechanism of enzyme action.
Cell culture work is also inseparable from it. Cells are extremely sensitive to the pH of their environment, and a slight deviation may affect cell growth, metabolism and even survival. As a buffer component of cell culture medium, CAPSO-Na can effectively regulate the pH value of the culture medium, create a suitable survival microenvironment for cells, and ensure normal cell growth and proliferation. It is widely used in cell biology research and biopharmaceutical cell culture.
In addition, CAPSO-Na also plays an important role in electrophoresis experiments. The electrophoresis process requires precise control of pH conditions to achieve efficient separation and analysis of biomacromolecules. It can stabilize the pH of the electrophoresis system, enabling the ideal separation of biomacromolecules such as proteins and nucleic acids according to their own charges and size differences, thus laying a good foundation for subsequent analysis and identification.

In the experiment, the optimal concentration of 3 - (cyclohexylamino) - 2 - hydroxy - 1 - propanesulfonic acid (Capso) sodium salt varies depending on the experimental system and purpose.
If the experiment is related to protein research, such as protein crystallization, activity determination, etc., the concentration of 0.05 - 0.2M is usually appropriate. Covering this concentration range can effectively maintain the pH stability of the system, and there is little interference with the structure and function of the protein, which helps the protein to maintain its inherent characteristics under near physiological conditions.
When it comes to enzymatic reaction experiments, the concentration of 0.1 - 0.3M is more commonly used. This concentration can provide a suitable micro-environment for the enzyme, stabilize the active center conformation of the enzyme, and enable the enzyme to fully exert its catalytic efficiency, while avoiding adverse effects on enzymatic reactions due to factors such as ionic strength.
In cell culture and other related experiments, considering the sensitivity of cells to the environment, the concentration is mostly controlled at 0.02-0.1M. This can not only adjust the pH of the culture medium, ensure that cells grow in a suitable pH environment, but also prevent high-concentration salts from having toxic effects on cells, and ensure normal cell growth and metabolism.
All in all, in order to determine the optimal use concentration of Capso sodium salt, researchers need to explore carefully and accurately through pre-experiments according to specific experimental needs, so as to obtain the ideal concentration suitable for specific experiments.

This is to investigate the stability of sodium salts of 3 - (cyclohexylamino) -2 -hydroxy-1 -propanesulfonic acid (CAPSO). As a commonly used buffer in biochemical research, the stability of CAPSO sodium salts is related to many experiments and applications.
As far as chemical stability is concerned, under conventional environments, this compound has certain stability. In its structure, the sulfonic acid group interacts with hydroxyl and amino groups to stabilize the molecular structure. However, under extreme acid-base conditions, the stability may be affected. In strongly acidic environments, amino groups or protons change the charge distribution and chemical properties of molecules; in strongly basic environments, hydroxyl groups may participate in the reaction, affecting their structure and function.
Temperature is also a key factor affecting its stability. Usually, under normal temperature conditions, CAPSO sodium salts can exist stably for a long time. However, when the temperature rises, the thermal motion of the molecule intensifies, or prompts chemical reactions to occur, resulting in decomposition or deterioration. If the heating temperature is too high and the time is too long, the chemical bonds within the molecule may break, changing its chemical structure and reducing its stability.
In addition, light will also play a role in its stability. Long-term strong light irradiation may lead to luminescent chemical reactions, resulting in molecular structure changes. Although the compound is not a typical photosensitive substance, it is still important to avoid direct exposure to strong light to maintain its stability.
In terms of storage, it should be placed in a dry, cool and dark place. Dry environment can prevent reactions such as hydrolysis caused by moisture; cool conditions can reduce the thermal movement of molecules and reduce the probability of chemical reactions; avoid photochemical reactions in the dark. In this way, the stability of CAPSO sodium salt can be maintained to the greatest extent, and its performance and effect in experiments and applications can be ensured.

3- (cyclohexylamino) - 2-hydroxy-1-propane sulfonate sodium salt, namely Capso, Sodium Salt, is a commonly used biological buffer. In the biochemical experimental environment, it encounters with many chemical substances, or reacts, or no reactions occur, depending on the specific material properties and reaction conditions.
If it encounters a strong acid, it is weakly basic, or an acid-base neutralization reaction may occur. The protons in the acid interact with the amino or hydroxyl groups in Capso and Sodium Salt, thereby changing the pH value and chemical composition of the solution. For example, when it encounters hydrochloric acid, the hydrogen ions in the hydrochloric acid will combine with the amino groups of Capso and Sodium Salt to form corresponding salts.
If there is a strong oxidizing agent, the hydroxyl group in the Capso and Sodium Salt molecules may be oxidized, causing the molecular structure to change. Like contacting with strong oxidizing agents such as potassium permanganate, the hydroxyl group may be oxidized to an aldehyde group, a carboxyl group, etc.
However, if it is with stable inert substances, such as some saturated hydrocarbons, due to their low chemical activity, it is usually difficult for Capso and Sodium Salt to react with them. And under the limitations of specific temperature and concentration, even if the theoretically reachable substances, or due to the extremely slow reaction rate, it is difficult to detect obvious signs of reaction within the conventional experimental time. Therefore, whether Capso, Sodium Salt and other chemical substances react requires comprehensive consideration of many factors such as material characteristics and environmental conditions.

3- (cyclohexylamino) -2-hydroxy-1-propanesulfonate sodium salt, there are indeed many safety precautions to be paid attention to when using this substance.
This substance is irritating. If it comes into contact with the skin, rinse it with a lot of water immediately, and then decide whether to seek medical attention according to the situation; if it comes into contact with the eyes, rinse it thoroughly with a lot of flowing water or normal saline quickly, and then seek medical attention. Due to the sensitivity of eye tissue, there is a slight carelessness or serious consequences.
In terms of operation, it should be carried out in a well-ventilated place. Operators need to wear appropriate protective equipment, such as protective gloves, goggles, etc., to avoid dust generation and inhalation. Due to dust or irritation of the respiratory tract, long-term inhalation or damage to the lungs. When storing, it should be placed in a cool, dry and well-ventilated place, away from fire and heat sources, and stored separately from oxidants and acids to prevent reactions and hazards.
Handle with care during handling to prevent damage to packaging and containers. In the event of a leak, the leaking contaminated area should be quickly isolated and personnel should be restricted from entering and leaving. Emergency responders should wear dust masks (full masks) and anti-acid and alkali work clothes. Do not allow leaks to come into contact with combustible substances (such as wood, paper, oil, etc.). In the event of a small leak, a clean shovel can be used to collect it in a dry, clean, covered container. For large leaks, embankments or pits need to be built for containment, and pumps should be transferred to tanks or special collectors for recycling or transportation to waste treatment sites for disposal. In this way, the safety of operation and use can be guaranteed.