What are the chemical properties of Ches 2- (N-Cyclohexylamino) Ethanesulfonic Acid
Ches is 2 - (N -cyclohexylamino) ethanesulfonic acid, which is a commonly used zwitterionic buffer in biochemical research. It has the following chemical properties:
First of all, this substance is a white crystalline powder with stable properties and is not easy to deteriorate under normal storage conditions. It has good solubility in water and can quickly dissolve to form a uniform solution. This property makes it extremely convenient to configure buffers.
From the perspective of acid-base characteristics, Ches has a pKa value of about 9.3, showing good buffering ability in the range of pH 8.6 - 10.0. In this pH range, when acidic or alkaline substances are added, it can effectively resist large changes in the pH value of the solution and maintain the stability of the pH of the system through the dissociation or combination of its own protons.
Ches is relatively stable chemically and generally does not react chemically with common metal ions and most organic solvents, so it is suitable for a variety of chemical and biological systems. However, in the presence of strong oxidizing agents or reducing agents, its structure may be damaged. In addition, extreme conditions such as high temperature and high humidity may also affect its stability. Due to its unique chemical properties, Ches is widely used in biochemistry and molecular biology experiments, such as protein purification, enzyme activity research, cell culture, etc., to provide a stable pH environment for related research and ensure the accuracy and reliability of experimental results.
Ches 2- (N-Cyclohexylamino) Ethanesulfonic Acid
Ches is 2- (N-cyclohexylamino) ethanesulfonic acid, which has a wide range of uses. In the field of biochemistry and molecular biology, it is often used as a buffer. Because of its specific acid-base properties, it can maintain the relative stability of the pH of the solution within a certain pH range, just like building a stable "base" for biochemical reactions, ensuring the smooth development of various enzymatic reactions, protein and nucleic acid research experiments, as if paving the way for fine biological experimental operations.
In cell culture work, Ches is also indispensable. Cell growth is extremely sensitive to environmental pH, and a slight deviation may affect its normal metabolism and proliferation. As a key member of the buffer system, Ches can maintain the pH of the cell culture environment at an appropriate range, creating a stable home for cells that resembles the original, and helping cells grow and pass on healthily, just like caring for delicate seedlings.
In addition, in the process of drug development, the stability and solubility of drugs are closely related to the pH of the environment. Ches can be used to simulate the pH of the human physiological environment in order to more accurately study the behavior of drugs, just like building a "test stage" close to the real scene of the human body for drugs, providing key support for drug development and optimization.
At the same time, in some chemical analysis and other fields, Ches can be used to adjust the pH of reaction systems or analysis systems due to its buffering properties, making the analysis process more accurate and reliable, like a precise "scale" for complex chemical analysis calibration. In short, Ches plays a crucial role in many scientific research and practical application scenarios with its unique buffering function.
What is the preparation method of Ches2- (N-Cyclohexylamino) Ethanesulfonic Acid
To prepare Ches (2- (N-cyclohexylamino) ethanesulfonic acid), you can do it according to the following method.
First take an appropriate amount of N-cyclohexylamine and place it in a clean reaction vessel. This amine has a cyclic structure and is a key starting material for the reaction. Next, slowly add bromoethanesulfonic acid dropwise to it. The bromine atom of bromoethanesulfonic acid has good departure properties and can undergo nucleophilic substitution reaction with the nitrogen atom of N-cyclohexylamine. The temperature control process is required to prevent overreaction. Generally speaking, temperature control at low temperature, such as 0-5 ° C, can make the reaction proceed smoothly.
Add dropwise, heat up to a moderate temperature, such as 30-40 ° C, and continue to stir to make the reaction sufficient. At this stage, the intermolecular reaction is gradually completed, and the nitrogen atom is connected to the carbon after the bromine atom leaves, forming the basic structure of the target product.
After the reaction is completed, the mixture contains products and unreacted raw materials. By distillation, low-boiling impurities can be removed first, and then further purified by column chromatography. Choose a suitable eluent, such as a mixture of dichloromethane and methanol in a suitable ratio, so that the product and impurities can be separated on the silica gel column. After elution, collection, concentration and other operations, pure 2- (N-cyclohexylamino) ethanesulfonic acid can be obtained. The entire preparation process requires fine operation and attention to the regulation of reaction conditions in order to obtain high-purity products.
What are the application examples of Ches 2- (N-Cyclohexylamino) Ethanesulfonic Acid in different fields
Ches, or 2- (N-cyclohexylamino) ethanesulfonic acid, is used in biochemical research and many fields.
In biochemical experiments, it is often used as a buffer. For example, in protein crystallization experiments, proteins are extremely sensitive to environmental pH, and Ches can precisely maintain the stability of the pH of the system, so that the protein is in a suitable crystallization environment. For example, when studying the structure of a membrane protein, Ches is used to create a stable pH environment, which promotes the successful crystallization of the membrane protein, so that the three-dimensional structure can be analyzed by X-ray crystallography.
Ches is also commonly used in the field of cell culture. When animal cells are cultured, cell growth requires strict pH requirements, and Ches can adjust and stabilize the pH value of the culture medium. For example, in the culture of neural stem cells, suitable pH is essential for their proliferation and differentiation. Ches plays a key role in the culture medium to ensure the normal growth and differentiation of neural stem cells, and to provide cell models for the research and treatment of nervous system diseases.
In the study of enzymes, Ches is also indispensable. The activity of enzymes is significantly affected by pH, and specific enzymes need a specific pH environment to exhibit the best activity. Taking the study of a key enzyme involved in drug synthesis as an example, Ches is used to formulate a buffer system to determine the optimal pH of the enzyme, improve its catalytic efficiency, and provide a basis for the optimization of drug synthesis processes.
In analytical chemistry, Ches can be used to adjust the pH of the analytical system to improve the accuracy and sensitivity of the analytical method. For example, when analyzing biological samples by high performance liquid chromatography, the pH of the mobile phase affects the separation effect of substances, and the addition of Ches adjusts the pH to achieve good separation and accurate determination of various components in complex biological samples.
What are the precautions when using Ches 2- (N-Cyclohexylamino) Ethanesulfonic Acid
Ches is 2- (N-cyclohexylamino) ethanesulfonic acid. The following matters should be paid attention to when using it.
First, pay attention to proper storage. Ches should be stored in a dry, cool and well-ventilated place, away from heat and fire sources. Because it is a chemical substance, if it is not stored properly, such as moisture or heat, it may cause its properties to change and affect the use effect. For example, in a humid environment, it is easy to absorb water, resulting in reduced purity.
Second, pay attention to solution preparation. When preparing Ches solution, its concentration needs to be precisely controlled. According to specific experimental or application needs, operate with the help of precise measuring tools, such as balances, pipettes, etc. Concentration deviations may have a significant impact on subsequent reactions or experimental results. If in the preparation of buffer solution, the concentration is not allowed to change the buffering capacity.
Third, pay attention to pH adjustment. Ches is often used as a buffer, and the pH value needs to be adjusted carefully according to the actual requirements. Different pH environments are suitable for different reactions or systems. When adjusting, it is advisable to slowly add acid-base reagents and monitor in real time with the help of a pH meter to prevent excessive adjustment.
Fourth, be careful and safe to operate. Although Ches is generally less toxic, it is still necessary to avoid direct contact with the skin and eyes. When operating, it is advisable to wear suitable protective equipment, such as gloves, goggles, etc. If it is accidentally touched, rinse with plenty of water immediately and seek medical assistance according to the specific situation.
Fifth, pay attention to compatibility. When using Ches, consider its compatibility with other chemicals. Certain substances may react with Ches and interfere with the experimental or production process. When using in a new system, it is best to conduct a small-scale compatibility test in advance.
