Diammonium 2,2'-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonate)

2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonate% 29 Dipotassium, the Chinese name is often called ABTS dipotassium salt, its main uses are as follows:
This is a commonly used reagent in the field of biochemistry and analysis. In biochemical research, it is mostly used for enzyme activity detection. For example, peroxidase detection, under the catalysis of peroxidase, ABTS dipotassium salt can be oxidized by hydrogen peroxide to produce products with color changes. This color change can be measured by spectrophotometer. According to the change of absorbance, the peroxidase activity can be accurately measured, which in turn helps to explore enzyme kinetics.
In the field of analytical chemistry, it is often used for colorimetric analysis. Because it reacts with specific substances to produce significant color changes, it can be used as a color developer. When detecting the content of certain substances, ABTS dipotassium salt reacts with it to generate a colored product, and the color depth of the product is determined by colorimetry to obtain the content of the substance. For example, in the quantitative analysis of specific enzymes or chemical components in some biological samples, it plays a key role. In addition, in immunoassay technology, it can also be used to assist in the detection of target substances by means of labeling, which can help to carry out accurate immunoassay.

2% 2C2% 27 -azido-bis (3-ethylbenzothiazoline-6-sulfonic acid) dipotassium (i.e. ABTS dipotassium), its working principle is as follows:
ABTS can be oxidized by a specific oxidase (such as horseradish peroxidase, etc.) or some oxidizing substances. In the presence of a suitable oxidation system, ABTS will lose electrons and undergo an oxidation reaction.
During its oxidation process, the molecular structure of ABTS changes, and the originally colorless or lighter-colored ABTS is transformed into an oxidized ABTS cationic radical with a strong color (usually blue-green) (ABTS • 🥰). This color change is very obvious, and there is a strong absorption peak at a specific wavelength.
By detecting the change of absorbance at a specific wavelength (generally 405nm, 414nm or 650nm, etc., depending on the experiment), the content of oxidizing substances in the process or system of oxidation reaction can be indirectly determined. For example, in enzyme-linked immunosorbent assay (ELISA), if the labeled enzyme is horseradish peroxidase, when the enzyme interacts with the substrate ABTS, the resulting color change can be used to determine the amount of target substance in the sample. The absorbance and the content of the target substance show a specific proportional relationship within a certain range, so as to achieve quantitative analysis. This principle is widely used in many fields such as biochemical analysis and immunoassay to detect related substances sensitively and conveniently.

2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonate% 29 Dipotassium, that is, ABTS dipotassium salt, has many advantages over other similar products.
First, the water solubility is very good. This characteristic allows it to dissolve quickly and uniformly in various aqueous systems, just like fish getting water, it can smoothly participate in many biochemical reactions without worrying about dissolution problems. This is the basis for the smooth development of many reactions. For example, in some enzymatic reactions that need to be carried out in an aqueous solution environment, its good water solubility ensures that the reactants are fully contacted and the reaction proceeds.
Second, the stability is quite strong. Under common experimental conditions, it can maintain the stability of its own chemical structure and properties for a long time, just like a sturdy fortress, and is not easily decomposed or deteriorated by external factors such as temperature, humidity, light, etc. This makes the experimental results more reproducible and reliable. Whether it is a short-term experiment or an experiment that requires a long time, it can provide stable and reliable data support.
Third, sensitive response. Once it encounters a specific reactant, it can react quickly if it encounters a confidant, and the reaction signal is significant, making it easy to observe and detect. In the field of detection, this advantage is obvious, enabling efficient and accurate detection of target substances, just like a keen hunter, who can accurately detect targets.
Fourth, safe and harmless. Whether it is to the experimental operator or to the environment, its toxicity and irritation are extremely low, just like a gentle gentleman, there is no need to worry too much about safety hazards during use, which greatly protects the health of the experimental personnel and reduces the potential harm to the environment.
In summary, 2% 2C2% 27 - Azino - Bis% 283 - Ethylbenzothiazoline - 6 - Sulfonate% 29 Dipotassium salts With the above advantages, occupy an important position in many fields such as biochemistry and testing, providing strong support for scientific research and production.

2% 2C2% 27 -azobis (3-ethylbenzothiazoline-6-sulfonic acid) dipotassium salt, that is, ABTS dipotassium salt, when using, many matters need to be paid attention to.
First, this substance is quite sensitive to light, and it is easy to decompose under light, which will affect the accuracy of the experimental results. Therefore, when storing and retrieving, it must be done under shading conditions, or stored in a brown bottle, sealed immediately after taking it, to avoid light.
Second, when ABTS dipotassium salt dissolves in water, the water temperature should be at room temperature, and hot water should not be used to prevent its structure from being damaged. During the dissolution process, it can be stirred gently to promote its instant dissolution, but the action should not be too violent to avoid excessive foam.
Third, this reagent is mostly used for biochemical testing, such as the determination of peroxidase and other activities. The pH value of the reaction system has a huge impact on the reaction results. Different experiments must precisely adjust the pH value according to the requirements to obtain reliable results.
Fourth, after the ABTS dipotassium salt solution is configured, it should not be left for a long time, because its stability is limited, over time, the color of the solution may change, and the activity will also decrease. Therefore, it is appropriate to use it now to ensure the accuracy of the experiment.
Fifth, during the operation, protective measures are indispensable. Although its toxicity is relatively low, it still avoids direct contact with the skin and eyes. In case of accidental contact, rinse with plenty of water immediately and seek medical attention if necessary.

2% 2C2% 27-azido-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, the storage conditions are as follows:
This substance should be stored in a cool, dry and well-ventilated place. Due to its nature or sensitivity to environmental factors, the temperature should be maintained between 2-8 degrees Celsius, so as to maintain the stability of its chemical structure and delay the rate of deterioration. Humidity also needs to be strictly controlled. Excessive humidity may cause it to absorb moisture and affect the quality. Therefore, the relative humidity of the storage environment should be maintained at 40% - 60%.
Furthermore, it needs to be placed in a dark place. Light or induced luminescent chemical reactions can change the molecular structure and damage the efficacy. When storing, also keep away from strong oxidants, strong acids and strong bases and other substances. These chemicals come into contact with it or cause violent reactions, causing it to fail and even endanger safety.
During storage, ensure that the packaging is tight. If the packaging is damaged, external air, moisture, etc. are easy to invade and accelerate its deterioration. After taking it, it needs to be sealed in time to maintain its quality. Store under these conditions to obtain its full integrity and maintain its effectiveness for a long time.