Dipso 3-(N,N-Bis[2-Hydroxyethyl]Amino)-2-Hydroxypropanesulfonic Acid

Dipso, or 3- (N, N-bis [2-hydroxyethyl] amino) -2-hydroxypropanesulfonic acid, is a commonly used biochemical reagent. It has the following chemical properties:
This substance is in the form of a white crystalline powder, which has good solubility in water and is slightly soluble in organic solvents. It can exhibit buffering properties in solution, and can effectively maintain the stability of the pH value of the system in biochemical experiments. The pKa value of Dipso is about 7.6, which determines that its buffering efficiency is excellent in the pH7.0-8.2 range. In many experimental scenarios such as enzymatic reactions and protein research, this pH range is quite critical because it is close to the physiological pH value in organisms, which can maintain the natural structure and activity of biomolecules.
In addition, Dipso is chemically stable, and it is not easy to react with other chemical substances under common experimental conditions, which greatly guarantees the purity and stability of the experimental system and does not interfere with the biochemical process studied. And it has little effect on biological macromolecules such as biofilms and proteins, and does not change the original properties and functions of biological macromolecules, so it is widely used in the field of biochemistry and molecular biology experiments.

Dipso, or 3- (N, N-bis [2-hydroxyethyl] amino) -2-hydroxypropanesulfonic acid, has important applications in many fields such as biochemistry and molecular biology.
In biological buffer systems, its role is crucial. Because of its specific acid-base dissociation constant, it can effectively maintain the stability of solution pH within a specific pH range. In the study of proteins and enzymes, proteins and enzymes are extremely sensitive to the pH of their environment, and small pH changes may affect their structure and function. The stable buffer environment constructed by Dipso can keep proteins and enzymes under suitable pH conditions, so as to maintain their natural conformation and biological activity, and ensure the accuracy and reliability of related experiments.
The field of cell culture is also its place of use. Cell growth requires strict requirements on the culture environment, and pH value needs to be strictly controlled. Dipso can create a stable microenvironment, provide suitable conditions for cell growth, and help cells to metabolize and proliferate normally. It is one of the key factors for the success of cell culture experiments.
In the process of nucleic acid research, whether it is DNA extraction, amplification, or RNA transcription, analysis, etc., it needs to be carried out under specific pH conditions. Dipso buffer can effectively maintain the pH stability of the system, avoid nucleic acid degradation or structural changes due to pH changes, and ensure the smooth progress of nucleic acid research experiments.
At the clinical diagnostic level, many diagnostic methods rely on a specific pH environment to ensure the accuracy and sensitivity of detection. As a buffer, Dipso can create suitable reaction conditions for diagnostic reagents, improve the reliability of diagnostic results, and play an indispensable role in the early and accurate diagnosis of diseases.

Dipso is 3- (N, N -bis [2-hydroxyethyl] amino) -2 -hydroxypropanesulfonic acid. It is extremely important to store this reagent properly, which is related to its chemical stability and accurate experimental results. According to the method of "Tiangong Kaiqi", it should be as follows.
The first environment is dry. Moisture is easy to cause deliquescence of the reagent and destroy its chemical structure. Therefore, a dry place should be selected, such as placed in a closed container containing the desiccant, and the humidity at the storage place should be controlled below 40%.
The second time is the appropriate temperature. High temperature can promote its chemical reaction to speed up the deterioration, and low temperature or crystallization affect the use. It is better to 15 ° C - 25 ° C. If it is very hot in summer, you can use an air conditioner to control the temperature. In cold winter, choose warm measures to keep it warm.
Furthermore, avoid strong light. Light or chemical reactions that lead to light damage its quality. It should be stored in a brown bottle and in a place without direct light, such as in a cabinet protected from light.
In addition, when using it, be sure to ensure that the appliance is clean and dry, free of impurities mixed in. After use, seal it immediately to prevent air and moisture from invading.
In this way, according to these storage methods, Dipso can be guaranteed to maintain a good chemical state for a long time for experimental needs.

To determine the purity of Dipso (3- (N, N -bis [2-hydroxyethyl] amino) - 2-hydroxypropanesulfonic acid), you can do it according to the following method.
First, high-performance liquid chromatography (HPLC) is used. Take an appropriate amount of Dipso sample, dissolve it in a suitable mobile phase and dilute it into a solution of a certain concentration. Select a suitable chromatographic column, such as a C18 column, and set the conditions of mobile phase flow rate and column temperature. After injection, determine the main peak according to the retention time, and measure its purity by area normalization method. This method can effectively separate impurities and main components, and obtain purity according to the ratio of peak area.
The second time is measured by titration. Take a certain amount of Dipso sample, add an appropriate amount of water to dissolve it, and titrate it with a standard alkali solution of known concentration. Take phenolphthalein as an indicator until the solution changes color and does not fade within half a minute as the end point. According to the volume and concentration of the standard alkali solution consumed, the content of Dipso is calculated according to the stoichiometric relationship, and then the purity is obtained.
Or use mass spectrometry (MS). The sample is ionized, and the ions are separated and detected according to the mass-to-charge ratio. Information such as the molecular weight of the sample can be obtained from the mass spectrogram, and the purity can also be inferred according to the comparison of the peak intensity with the standard.
Infrared spectroscopy (IR) can also be used as an auxiliary method. Make a suitable sample of Dipso and measure its infrared spectrum Compared with the standard infrared spectrum, the presence or absence of impurities and the approximate purity can be judged according to the position and intensity of the characteristic absorption peaks. Through the comprehensive application of various methods, the purity of Dipso can be accurately determined.

To prepare Dipso, that is, 3- (N, N-bis [2-hydroxyethyl] amino) -2-hydroxypropanesulfonic acid, the preparation method is as follows:
First take an appropriate amount of raw materials, with ethylene oxide and N-methyldiethanolamine as starting materials. In a suitable reaction vessel, control the reaction temperature and pressure. The temperature should be maintained in a specific range, which needs to be precisely regulated. If the temperature is too high or too low, it may affect the reaction process and product purity. The pressure also needs to be stable at a certain value to make the reaction proceed smoothly.
During the reaction process, a specific catalyst is added, which can effectively promote the reaction rate and reduce the activation energy required for the reaction. The amount of catalyst needs to be precisely controlled, too much or too little is not conducive to the reaction. Ethylene oxide reacts with N-methyldiethanolamine under the action of the catalyst to form an intermediate product.
Then, the intermediate product is further processed. The intermediate product is reacted with a specific sulfonating reagent, and the type and amount of sulfonating reagent need to be strictly controlled. Reaction conditions such as temperature and reaction time have an important impact on the formation of the final product.
After the reaction is completed, the product is separated and purified. A variety of separation methods can be used, such as filtration, extraction, distillation, etc., to remove residual impurities, unreacted raw materials and by-products in the reaction system. Through multiple purification operations, the purity of the product is improved until it reaches the required standard, so that pure Dipso can be obtained. The whole preparation process requires fine control of the reaction conditions, raw material ratio and operation process of each step to ensure the quality and yield of the product.