Hepes, Hemisodium Salt N-(2-Hydroxyethyl)Piperazine-N-(2-Ethanesulfonic Acid), Hemisodium Salt

Lingxian Chemical

Specifications

HS Code	781522
Chemical Formula	C8H17N2NaO6S
Molar Mass	292.28 g/mol
Appearance	White to off - white powder
Solubility	Soluble in water
Ph Range For Buffer	6.8 - 8.2
Pka At 25 C	7.55
Storage Condition	Store at room temperature, keep container tightly closed
Stability	Stable under normal conditions
Density	N/A (powder)
Cas Number	106923-33-9

Packing & Storage

Packing	Hepes hemisodium salt in 500g package, suitable for chemical applications.
Storage	Store Hepes, Hemisodium Salt in a cool, dry place, away from heat sources. Kept in a tightly - sealed container to prevent moisture absorption and degradation. Avoid exposure to strong oxidizing agents. Recommendations might include storing at a temperature range of 2 - 8°C if long - term stability at low temperatures is beneficial for this biological buffer.
Shipping	Hepes, Hemisodium Salt is shipped in tightly - sealed containers, safeguarded from moisture and environmental contaminants. Shipment follows strict chemical - transport regulations to ensure safe and damage - free delivery.

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Hepes, Hemisodium Salt N-(2-Hydroxyethyl)Piperazine-N-(2-Ethanesulfonic Acid), Hemisodium Salt

General Information

Historical Development

Hepes, Hemisodium Salt, that is, N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt, the development of this substance can be traced back to the past. At the beginning, the academic community was still shallow in its understanding, and only a little bit of its basic properties were known. After unremitting research by various scholars, it gradually developed its edge in the field of biochemistry. It has a significant effect in maintaining the stability of the pH of the solution, just like the needle of the sea, providing a stable environment for many experiments. With the passage of time, the technology has become more and more mature, and the preparation process has been continuously refined, from the initial crude to the current sophisticated. Its application is also becoming more and more extensive, and it is indispensable in cell culture, biological agents, and many other aspects. Just like the stars illuminating the road of scientific research, it promotes the steady progress of biochemical research and becomes a shining key element in the scientific research process.

Product Overview

Today there is a product named Hepes, Hemisodium Salt, that is, N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt. This is a commonly used product in chemical research. It is stable in nature and has many applications in biochemical experiments. It can be used as a buffer to maintain a stable pH of the system, ensure a suitable experimental environment, and make the reaction smooth. Its shape may be powder, with pure color and uniform quality. In the process of scientific research, many experiments rely on its assistance, just like a boat needs a good river and sea. It is important for researchers to help explore the mysteries of chemistry and is of great value in the field of biochemical research.

Physical & Chemical Properties

Hepes, Hemisodium Salt, is the hemisodium salt of N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid). Its physical properties are unique. It is mostly white crystalline powder at room temperature, with good solubility, and can be rapidly dispersed in common solvents such as water. In terms of chemical properties, this substance is relatively stable in chemical properties, and can be used as a buffer within a certain pH range to effectively maintain the pH of the system. Due to its special structure, it has a wide range of uses in biochemical experiments and other fields. It is often used in cell culture and other environments. It can provide a stable acid-base environment for biological reactions, help experiments to carry out smoothly, and ensure the normal functioning of bioactive substances. It is an indispensable and important chemical substance in biochemical research.

Technical Specifications & Labeling

Today there are chemicals called Hepes, Hemisodium Salt, which is N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) hemisodium salt. Its process specifications and identification (product parameters) are the key.
The process specifications need to be accurate, related to the purity of the ingredients, and the impurity content must be strictly limited to ensure its excellent quality. The production process should also be standardized and orderly. From the selection of raw materials to the synthesis steps, there are rules and no mistakes.
In terms of identification, the product name should be clear and accurate. If the above name is used, it cannot be confused. Parameter labeling should be complete, and characteristics such as molecular weight, purity value, and solubility should be detailed so that users can see it at a glance to ensure that the chemical can be applied correctly in scientific research, production, and other fields to achieve its due effect.

Preparation Method

The method of preparing Hepes, Hemisodium Salt (N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Hemisodium Salt) requires first preparing all raw materials. Take the appropriate reactant in a specific ratio, where the purity of the raw material is crucial and the quality of the product is related.
During the reaction, the temperature, pressure and reaction duration are controlled according to carefully set process steps. In a specific container, the reactants are mixed in sequence to ensure uniform contact and sufficient reaction. And a delicate catalytic mechanism needs to be set up, and a suitable catalyst is selected to improve the reaction rate and yield.
Each step must be carefully carried out, and the reaction progress must be monitored in real time according to the standard process. In this way, high-quality Hepes, Hemisodium Salt can be prepared to meet all needs.

Chemical Reactions & Modifications

Hepes, hemisodium salt, that is, N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt, the chemical reaction and modification of this substance are related to many wonders. In biochemical reaction systems, it is often used as a buffer, which can stabilize the acid and base of the system, just like the needle of the sea, to protect the smooth reaction. The characteristic of its reaction is that it can adjust itself skillfully according to changes in the environment, so as to achieve the balance between acid and base. As for modification, it can be modified by chemical modification to add functional groups, change its hydrophilic and hydrophobic properties, or change its molecular structure, increase its stability, so that it can be used in a variety of biochemical scenarios, and add a lot to biochemical research and application.

Synonyms & Product Names

Hepes, the hemisodium salt (N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid), hemisodium salt) This substance is widely used in the field of biochemistry. Its synonyms are also important to researchers.
Guanfu's ancient books, there are many other names. Or hydroxy ethylpiperazine ethanesulfonate sodium hemisodide, or variants of this name, all refer to this substance. Although the names are different, they are actually the same, all are Hepes, and the hemisodium salt is also. The names sold by merchants are different, but in fact, one is also.
Researchers use it mostly because it can stabilize the acid and base of the solution, and it is indispensable for biochemical reactions. Because of its synonym, when buying, it must be carefully reviewed to avoid mistaken purchase. It is essential to clarify its synonym and trade name in biochemical research.

Safety & Operational Standards

There are chemical products today, named Hepes, Hemisodium Salt, which is N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt. The safety and operating specifications of this product are of great importance and must not be ignored.
When handling this product, clean your hands and face, wear plain work clothes, wear a protective cap, and pedal your feet to prevent staining. In a well-ventilated place, avoid pyrotechnics, heat, and avoid its qualitative change.
When taking it, the utensils must be clean and dry. Measure accurately, and do not make deviations. Accidentally splash on the body and skin, quickly rinse with water, if it enters the eyes, especially rush, and seek medical attention immediately.
When storing, it should be placed in a cool and dry place, away from water and fire, and tightly sealed. It should also be stored separately from other things to prevent them from interfering and causing changes.
After use, the rest should not be discarded at will, and disposed of in accordance with regulations. The utensils used should be cleaned in time for later use.
In short, those who use this thing should be in awe and strictly abide by the norms, so that the security will be safe and everything will go well.

Application Area

Hepes, Hemisodium Salt, namely N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) hemisodium salt, has a wide range of application fields. In biochemical research, it can be used as a buffer to maintain the stability of the pH of the system and ensure the smooth progress of biochemical reactions. In the field of cell culture, it can create a suitable acid-base environment for cells and promote cell growth and reproduction. When developing pharmaceuticals, as an important excipient, it contributes to the stable preservation and effective delivery of drugs. With its unique chemical properties, this compound plays a key role in many fields, providing strong support for scientific research and practical application, and has become an indispensable substance for modern scientific research and production.

Research & Development

I am dedicated to the research of Hepes, Hemisodium Salt. This is the compound of N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Hemisodium Salt. It is quite important in biochemical research.
At the beginning, the preparation method was not good, and the quality was uneven. Yu et al traced the source to explore the mechanism of the reaction and the conditions for adjusting the sub. After repeated tests and improved processes, the purity and yield were improved simultaneously.
Its properties were studied again, and its stability and pH regulation were observed in different environments. After long-term research, its characteristics were clarified, which laid the foundation for practical application. Although this road of research is full of thorns, everyone is pleased with every progress. It is hoped that this achievement will be beneficial to the biochemical fields and promote their development.

Toxicity Research

The toxicity of Hepes, Hemisodium Salt, N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Hemisodium Salt, has been investigated in depth. In various experiments, the toxicity of this substance has been found, which is related to the health of living beings, and cannot be ignored.
Those who study it, observe its reaction in different doses and environments, and hope to understand its harm to the body. Or observe its damage to cells, or observe its metabolism in living organisms, are all ways to understand toxicity.
Although the investigation has not been completed, the knowledge obtained has shown its signs of toxicity. It is of great benefit for future generations to use this thing or prevent its harm. When using this thing, future generations can be cautious, weigh the pros and cons, and avoid disasters.

Future Prospects

I try to study Hepes, Hemisodium Salt, a chemical substance. It is N- (2 -Hydroxyethyl) Piperazine-N- (2 -Ethanesulfonic Acid), a genus of Hemisodium Salt. Looking at the present, it has been widely used and has many benefits in biochemical experiments. However, my heart looks to the future, hoping that it can be better expanded. I hope that in medical research and development, it can help create new drugs and heal patients; in biotechnology, it can promote technological refinement and open up new horizons. Although achievements have been made today, the road to the future should shine in many fields, pave the way for the advancement of science and the well-being of mankind, and become an extraordinary cause.

Frequently Asked Questions

Hepes, Hemisodium Salt N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), What is the chemical structure of Hemisodium Salt?

Hepes, hemisodium salt, that is, N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt, its chemical structure is as follows:
The main structure of this compound is composed of a piperazine ring. One nitrogen atom on the piperazine ring is connected to 2-hydroxyethyl, and the other nitrogen atom is connected to 2-ethanesulfonic acid. The sulfonic acid group is bound to the sodium ion in the form of a hemisodium salt.
From the perspective of the piperazine ring, it is a six-membered nitrogen-containing heterocycle with certain stability and rigidity. The hydroxyl group of 2-hydroxyethyl is hydrophilic and can participate in the formation of hydrogen bonds, The sulfonate of 2-ethane sulfonate group is a strong acidic group with good water solubility and ionization ability, which can enhance the stability and reactivity of the compound in aqueous solution. The semi-sodium salt form reflects its acid-base balance characteristics. The sodium ion partially binds to the sulfonate group, which not only maintains a certain acidity, but also increases the stability and solubility of the compound due to the presence of sodium ion. The overall structure endows the compound with an important role in the biological buffer system, which can effectively maintain the pH stability of the system and is widely used in biochemical and cell biology experiments.

Hepes, Hemisodium Salt N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Hemisodium Salt What are the usual application fields?

Hepes, the hemisodium salt, namely N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt, has a wide range of uses. In the field of cell culture, this is an indispensable buffer. The cell growth environment is extremely sensitive to pH value, and small fluctuations may affect the cell state. Hepes hemisodium salt can effectively maintain the pH value of the medium, create a suitable growth environment for cells, and help the normal metabolism and proliferation of cells.
In biochemical research, many biochemical reactions require strict pH levels. This substance can precisely regulate the pH value of the reaction system to ensure the smooth progress of enzymatic reactions, protein purification and other experiments, providing guarantee for accurate experimental results.
Furthermore, in the field of drug development, whether it is the in vitro screening of drug active ingredients or the study of drug stability, a stable pH environment is essential. Hepes hemisodium salt can simulate the pH of the human physiological environment, help researchers better explore drug properties, and accelerate the drug development process. In short, Hepes hemisodium salt plays a key role in many fields of life science, and is of great significance in promoting related research progress.

What are the storage conditions for Hepes, Hemisodium Salt N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Hemisodium Salt?

Hepes, the hemisodium salt, that is, N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt, its storage conditions are very critical. This substance should be stored in a cool, dry and well-ventilated place.
Because of the cool environment, it can avoid the change of its properties caused by high temperature. High temperature often causes the internal structure of the compound to change, or cause decomposition and deterioration, which damages its chemical activity and purity. And dry places can prevent it from being damp. Moisture can easily lead to many chemical reactions, such as hydrolysis, which can destroy the original structure and properties of the substance. Furthermore, good ventilation can disperse the volatile substances that may accumulate in time, ensure its quality and stability, and prevent the accumulation of harmful gases, so as to avoid potential safety hazards.
When storing, also pay attention to separate from other chemicals. Different chemicals have different properties, and they are easy to cause chemical reactions when mixed together, or cause risks such as combustion and explosion. And it needs to be placed out of the reach of children. Because many chemicals are potentially dangerous, accidental touch and ingestion can cause serious consequences.
In addition, the packaging must be tight. In good condition, the packaging can effectively isolate the outside air, moisture and impurities, ensuring that the Hepes semi-sodium salt remains stable for a long time. After taking it, it should be sealed as soon as possible to prevent external factors from affecting its quality. In this way, the Hepes semi-sodium salt can be properly preserved to ensure its performance and quality.

Hepes, Hemisodium Salt N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), How stable is Hemisodium Salt in solution?

Hepes, the hemisodium salt, namely N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) hemisodium salt, is in solution, and its stability is very critical. This substance is often used in the fields of biochemistry and cell culture, and stability has a deep impact on its effectiveness.
In a conventional solution environment, if the pH value is in an appropriate range, generally about 6.8-8.2, Hepes hemisodium salt can be relatively stable. This is because of the specific functional groups in its structure, within this pH range, it can maintain its own chemical structure intact and does not easily decompose or react. For example, in cell culture fluids, under suitable pH conditions, it can effectively control the pH of the solution and create a stable living environment for cells.
However, the temperature of the solution will also affect its stability. If the temperature is too high, such as exceeding 37 ° C, it may cause molecular movement to intensify, cause structural changes, and reduce stability. Extreme low temperatures, although they can slow down molecular movement, may cause crystals to precipitate, which also affects its stability and original properties in the solution.
In addition, if other chemicals are present in the solution and interact with them, they may also change the stability of Hepes hemisodium salts. Like some metal ions, or form complexes with the substance, affecting its chemical properties and stability.
In conclusion, the stability of Hepes semi-sodium salt in solution is influenced by many factors such as pH value, temperature, and other solutes. When using, these conditions need to be carefully controlled to ensure its effectiveness.

Do Hepes, Hemisodium Salt N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Hemisodium Salt have common reactions with other chemicals?

Hepes, that is, N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) hemisodium salt, is quite commonly used in biochemical research and is mostly used as a buffer. The most common chemical substances that react with it are various acids and bases. Due to its buffering properties, when exposed to acid, the sulfonate in Hepes hemisodium salt will receive protons and react to form corresponding acid salts; when exposed to base, the hydroxyl group of hydroxyethyl will release protons and react with it to form corresponding salts and water, thereby maintaining the pH stability of the system.
In addition, metal ions can also react with Hepes hemisodium salt. Like divalent copper ions, zinc ions, etc., can coordinate with nitrogen and oxygen atoms in Hepes molecules to form complexes. This reaction is of great significance in the study of the biological activity of metal ions and the interaction between proteins and metal ions.
Also, Hepes hemisodium salts can react with some organic compounds with active groups. For example, compounds containing carboxyl groups can esterify with Hepes' hydroxyl groups under appropriate conditions; compounds containing amino groups can react with sulfonic acid groups and other reactions, which may have applications in organic synthesis and drug development.
It should be noted that the specific reaction situation will be affected by reaction conditions, such as temperature, concentration, pH value, etc. Under different conditions, the rate of reaction and the product may vary.