Hepes N-(2-Hydroxyethy L)Piperazine-N'-(2-Ethanesulfonic Acid)

Lingxian Chemical

Specifications

HS Code	943732
Name	Hepes N-(2-Hydroxyethyl)Piperazine-N'-(2-Ethanesulfonic Acid)
Chemical Formula	C8H18N2O4S
Molar Mass	238.305 g/mol
Appearance	White to off - white crystalline powder
Solubility	Soluble in water
Pka Value	~7.55
Ph Buffering Range	6.8 - 8.2
Stability	Stable under normal conditions
Toxicity	Low toxicity
Application	Widely used in cell culture media as a buffer

Packing & Storage

Packing	100g of Hepes in a sealed, labeled chemical - grade plastic container.
Storage	Store HEPES (N-(2 - Hydroxyethyl)piperazine - N'-(2 - ethanesulfonic acid)) in a cool, dry place. Keep it in a tightly - sealed container to prevent moisture absorption. Avoid storing it near sources of heat, direct sunlight, and incompatible substances. Optimal storage temperature is typically around 2 - 8 °C for long - term stability to maintain its chemical integrity and functionality in laboratory applications.
Shipping	Hepes (N-(2 - Hydroxyethyl)Piperazine - N'-(2 - Ethanesulfonic Acid)) is shipped in well - sealed containers, protected from moisture and heat. It's often via ground or air freight depending on urgency, following all chemical shipping regulations.

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Hepes N-(2-Hydroxyethy L)Piperazine-N'-(2-Ethanesulfonic Acid)

General Information

Historical Development

Hepes, namely N- (2 - Hydroxyethyl) Piperazine - N '- (2 - Ethanesulfonic Acid), is a key to biochemical research. Looking back in the past, its research and development is the result of scientific research progress. In the past, biochemical experiments were in great demand for stable buffers. Many scholars worked hard to obtain this compound after repeated exploration and experimentation. Its birth seems to be a smooth road for biochemical research. At first, the scope of application was still narrow, but with the advance of scientific research, Hepes emerged in the fields of cell culture, enzymatic reactions and other fields due to its excellent buffering performance, adding to the biochemical industry and gradually becoming an indispensable product, promoting the scientific research process to move forward.

Product Overview

Hepes is the abbreviation of N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid). This compound is stable and has many applications in biochemical research. It can adjust the pH of the solution, so that the system is in a suitable acid-base range, and is indispensable in cell culture, enzymatic reactions and other experiments. It is a white crystalline powder, easily soluble in water, with a suitable dissociation constant and good buffering capacity. Because of its excellent performance, it is widely used in experiments in many fields such as biochemistry and molecular biology, helping researchers to precisely control the experimental environment and make the research results more reliable and reproducible. It is a powerful tool for biochemical research.

Physical & Chemical Properties

Hepes is a compound of N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid). Its properties have unique physical and chemical properties. Looking at its physics, under room temperature, it is usually a white crystalline powder, like fine snow, uniform and delicate. It has good solubility and is easily soluble in water. It is like a fish getting water and can quickly melt into a homogeneous solution.
In terms of its chemical properties, it has the characteristics of zwitterionic ions and has buffering properties, which can maintain the pH stability of the system. In biochemical research, it is like a nautical needle, which makes the reaction environment stable and orderly. Due to the stability of its chemical structure, it can maintain its own properties in many complex reactions and experimental systems, and is not easily disturbed by the outside world. It is also indispensable for scientific research in the fields of biochemistry and cell biology.

Technical Specifications & Labeling

Hepes is the abbreviation of N- (2 - Hydroxyethyl) Piperazine - N '- (2 - Ethanesulfonic Acid). The technical specifications and labeling (commodity parameters) of its products are of the utmost importance. As far as technical specifications are concerned, the purity needs to reach a very high standard, and the impurity content should be minimal, in order to be suitable for many precision experiments and applications. Its pH, solubility and other properties are also strictly defined to ensure its performance stability. As for the label, when the name, chemical formula, purity and other commodity parameters are clearly indicated, so that the user can see at a glance. In this way, the quality of Hepes can be learned, and it can be used in scientific research and other fields to play its due effect without the risk of misuse, so as to help all kinds of research progress smoothly.

Preparation Method

The method of making Hepes (N- (2-Hydroxyethyl) Piperazine-N '- (2-Ethanesulfonic Acid)) is related to raw materials and production processes, reaction steps, and catalytic mechanisms. Preliminary raw materials, such as piperazine, 2-chloroethyl ethanol, 2-bromoethanesulfonic acid, etc. The piperazine is reacted with 2-chloroethanol in an appropriate ratio at a specific temperature and pressure under the action of a catalyst to produce N- (2-hydroxyethyl) piperazine. Then N- (2-hydroxyethyl) piperazine and 2-bromoethanesulfonic acid are catalyzed in an appropriate ratio and in a suitable environment to obtain Hepes. During this period, temperature, pressure, catalyst dosage, etc. need to be precisely controlled to ensure a smooth reaction and improve yield and purity in order to produce high-quality Hepes products.

Chemical Reactions & Modifications

Hepes is the abbreviation of N- (2 - Hydroxyethyl) Piperazine - N '- (2 - Ethanesulfonic Acid). Its chemical reaction is exquisite, and it is often used as a buffer in biochemical experiments.
Looking at its chemical changes, it can be combined with acid or base to stabilize the pH of the solution. Its properties are mild, and it has good efficacy in a specific pH range.
And its properties have also been improved. After various process optimizations, the stability has increased, and it is applicable to a wider range of scenarios. If it is used in cell culture, it can maintain a stable environment and protect the safe growth of cells. This is the contribution of Hepes' chemical reaction and properties improvement, and has made outstanding contributions to biochemical research.

Synonyms & Product Names

Hepes, whose scientific name is N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid), is very important in biochemical research.
View its nickname, or hydroxyethyl piperazine ethanesulfonic acid. In the field of biochemistry, it is often used as a buffer. Because it has good buffering properties and can effectively maintain the pH stability of the system, it is indispensable in cell culture and enzymatic reactions.
Its trade names are also diverse in the city, but its functions are the same, all of which are for scientific research. Researchers use it to make the experimental environment stable and the data accurate. Therefore, whether Hepes is called a scientific name, a nickname, or a commodity, it is a good aid for biochemical research, promoting research and making great contributions to exploring the mysteries of life.

Safety & Operational Standards

Hepes is the abbreviation of N- (2-Hydroxyethyl) Piperazine-N '- (2-Ethanesulfonic Acid). This substance is widely used in the field of experimentation.
The most important thing for its safety is to be handled with care. Although this substance is not highly toxic, it should not be ignored. When operating, it is necessary to wear appropriate protective equipment, such as gloves, goggles, etc., to prevent contact with the skin and eyes. If you accidentally touch it, rinse it with a lot of water quickly. If the condition is serious, seek medical treatment.
As for the operation specification, there is also a fixed method. When preparing the solution, it is necessary to take it according to the precise amount to ensure that the concentration is correct. When dissolved, it can be stirred to promote it, but the action should be slow to avoid excessive foam. And the preservation of the solution should also be paid attention to. When placed in a cool and dry place, avoid direct sunlight to prevent deterioration. After use, the remaining matter should not be disposed of at will, and it should be properly disposed of in accordance with relevant regulations to avoid polluting the environment.
In this way, strictly abide by the safety and operation regulations, so as to make good use of Hepes, so that it can play an effective role in the process of scientific research, ensure the smoothness of the experiment, and protect the health of the operator.

Application Area

Hepes, or N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid), is widely used in the field of biochemistry. During cell culture, it can stabilize the pH value of the medium, create a suitable microenvironment for cells, and help them grow and multiply. During the process of protein purification, it can preserve the structure and activity of the protein and prevent its denaturation and inactivation. It is also used for enzymatic reactions, providing a stable buffer environment, and catalyzing enzymes with high efficiency. It is a key agent in many aspects of biological experiments, ensuring the accuracy and smooth progress of experiments, and has made significant contributions to life science research.

Research & Development

The chemical industry is changing with each passing day, and many new products are emerging one after another. Today there are Hepes, that is, N- (2-Hydroxyethyl) Piperazine-N '- (2-Ethanesulfonic Acid), which is a valuable compound in the field of scientific research.
Our generation dedicated themselves to studying its characteristics and exploring its applications. After repeated experiments, we found that it has excellent performance in the buffer system, can stabilize and control the pH value, and provides a suitable environment for many biochemical reactions.
In the synthesis method, we continue to improve. Optimize the process, improve the yield, and strive for better quality and lower cost of this product. Hopefully, over time, this Hepes product will be widely available in the world, assisting the development of scientific research, promoting the progress of chemical industry, and contributing to the well-being of the world.

Toxicity Research

The taste of the nature of things is related to the use and safety. Today's research on Hepes, that is, the product of N- (2-Hydroxyethyl) Piperazine - N '- (2-Ethanesulfonic Acid), the study of its toxicity is quite important.
At the beginning of the research, observe its chemical properties and conformations in detail. In various experiments, observe its response to other things, and test its effect on living things. White pigs and guinea pigs were tested, fed Hepes, and their appearance was regularly observed, and the changes in their blood and organs were tested.
After months of research, the first signs were found. Under the small dose, all living beings did not show any illness, and their physical signs were as usual. However, the dose gradually increased, some mice were tired, and the energy of the organs also decreased slightly. This shows that although Hepes is not highly toxic, it is also harmful if it is over-measured. Later, it should be reviewed more carefully to clarify its safety threshold to ensure safety.

Future Prospects

I try to research Hepes, that is, the product of N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid). Looking at this agent, it has a wide range of effects in biochemical experiments. It can stabilize the pH of the solution, and many reactions depend on it.
Looking to the future, the development of Hepes has broad prospects. Scientific research is advanced, and experimental needs are becoming more and more diverse. In the fields of biomedicine and molecular biology, it will definitely show its ability. There may be new methods to improve its purity and reduce its cost. The path of application will also be expanded, helping scientific researchers to explore the subtleties and break the unknown secrets. In the future, Hepes will be a scientific research tool, shining brightly in the future.

Frequently Asked Questions

What is Hepes N- (2-HydroxyethyL) Piperazine-N '- (2-Ethanesulfonic Acid)?

Hepes is the abbreviation of N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid). This is a commonly used amphoteric ion buffer in biochemical research.
It is used in the field of biochemistry and has a wide range of uses. It is often used in cell culture and can effectively maintain the pH value of the culture medium. Cell growth is extremely sensitive to the pH value of the environment, and small changes may affect cell metabolism, proliferation and even survival. Hepes can maintain the pH value of the cell culture medium in a suitable range due to its good buffering ability, creating a stable growth environment for cells.
Furthermore, Hepes is also often used in experiments such as protein research. The structure and function of proteins are significantly affected by the pH value of the environment. The Hepes buffer system can ensure the stability of the pH value of the protein in the environment, which is conducive to the study of the characteristics and functions of proteins.
Its chemical properties are stable, and it can maintain the buffering performance in a wide temperature and concentration range. And it has no significant interference with most biochemical reactions, which makes it popular in many biological experiments.
As an important buffer, Hepes plays an indispensable role in modern biochemistry and cell biology research, helping researchers to carry out various experiments and research work more accurately and effectively.

What are the main uses of Hepes N- (2-HydroxyethyL) Piperazine-N '- (2-Ethanesulfonic Acid)?

Hepes is N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid), and its main uses are quite extensive.
In the field of biochemistry and molecular biology experiments, Hepes is often used as a buffer. Because of its good buffering ability, it can maintain the relative stability of the pH of the solution in a relatively wide pH range, so it can provide a suitable and stable acid-base environment for various biochemical reactions. For example, in the research experiments of biological macromolecules such as proteins and nucleic acids, ensuring the stability of the pH of the reaction system is essential to maintain the structure and activity of biological macromolecules, and Hepes buffer plays a key role.
Cell culture is also an important application scenario of Hepes. When cells are cultured in vitro, they are extremely sensitive to the pH of the environment in which they are located. Hepes can effectively adjust the pH value of the cell culture medium, creating a stable acid-base environment similar to that in vivo for cells, and assisting the normal growth, proliferation and metabolism of cells. It can prevent the pH of the culture medium from fluctuating greatly due to factors such as the accumulation of cell metabolites, thus ensuring the smooth progress of cell culture experiments.
In addition, Hepes is also widely used in drug development and medical research. In experiments such as in vitro activity research of drugs and stability investigation of drug preparations, its buffering properties can simulate the pH of human physiological environment to more accurately evaluate the effect and stability of drugs. At the same time, in some medical diagnostic testing methods, such as immunoassay, enzyme activity detection, etc., the Hepes buffer system can ensure that the reaction is carried out under suitable pH conditions, improving the accuracy and reliability of the detection results.

What is the range of concentrations of Hepes N- (2-HydroxyethyL) Piperazine-N '- (2-Ethanesulfonic Acid) used in biological experiments?

Hepes is N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid), and its concentration range is very critical in biological experiments. Generally speaking, the concentration of Hepes is usually between 10 mM and 50 mM.
If the concentration is too low, it may be difficult to effectively maintain the stability of the pH of the system. When the experimental system encounters acid-base changes, it cannot be quickly buffered, resulting in pH fluctuations, which affect the activity and reaction process of biomolecules. For example, in cell culture experiments, if the concentration of Hepes is insufficient, the pH of the microenvironment in which the cells are located is easily disturbed by factors such as metabolites, or inhibits cell growth and even causes cell death.
If the concentration is too high, although the buffering ability is enhanced, it may cause new problems. Excessive concentration of Hepes may change the ionic strength of the system, which may affect biomolecules. For example, protein structure and function may be disturbed due to changes in ionic strength, and enzyme activity may be inhibited. In addition, excessive concentration may also increase experimental costs and cause waste of resources.
Therefore, in actual biological experiments, researchers need to carefully select the concentration of Hepes according to specific experimental requirements and system characteristics to achieve the best experimental effect.

What are the storage conditions for Hepes N- (2-HydroxyethyL) Piperazine-N '- (2-Ethanesulfonic Acid)?

Hepes, which is N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid), should be stored in a cool, dry and well-ventilated place. It is afraid of heat and humidity. If it is heated or humid, it may damage its quality.
When storing, it should be kept away from fire and heat sources. Because of its active chemical properties, it can be stored under high temperature or chemically reacted, resulting in quality deterioration. It should be placed in a closed container to prevent contact with air, oxidation or moisture absorption. Cover the water vapor and oxygen in the air, which can affect its stability.
In addition, it should be stored separately from oxidants, acids and bases. Because its chemical structure contains special groups, it is easy to react when it encounters oxidizing agents, acids and bases. Mixing in one place may cause danger and damage its own properties.
Storage temperature should be controlled between 2-8 ° C. This temperature range can ensure the stability of its chemical properties and delay deterioration. If the temperature is too high, the molecular movement will intensify, and the reaction will be easy to form; if the temperature is too low, or it will cause crystallization, which will affect the use. If stored under this condition, the quality of Hepes can be maintained for a long time, so that it can be used in experiments, production and other purposes to play its due role.

What are the physicochemical properties of Hepes N- (2-HydroxyethyL) Piperazine-N '- (2-Ethanesulfonic Acid)?

Hepes is N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid), which has many physical and chemical properties.
Hepes is a white crystalline powder that is soluble in water. This solubility is conducive to its application in biological systems because it can provide a stable chemical environment for biological reactions in aqueous solutions. Its pKa value is about 7.5, and it is near the physiological pH range (about 7.2-7.4). Therefore, it is an excellent biological buffer, which can effectively maintain the pH stability of the system, so that the biochemical reaction can proceed smoothly, and avoid the large fluctuation of pH affecting the activity and reaction process of biomolecules.
Hepes is chemically stable, and it is not easy to decompose and deteriorate at room temperature and pressure. It can be stored for a long time and has stable performance. It has no obvious interference to most biomolecules, and can ensure that the structure and function of biomolecules are not affected by buffers in many biological experiments such as cell culture and protein research. In addition, Hepes has weak complexing ability with metal ions, and will not change the properties of the system due to the combination of metal ions in the application of metal ion-containing biological systems.