Hepes-Na-N-(2-Hydroxyethyl)Piperazine-N'-2-Ethanesulfonic Acid,Sodium Salt

Lingxian Chemical

Specifications

HS Code	711254
Chemical Formula	C8H17N2NaO4S
Molar Mass	260.29 g/mol
Appearance	White to off - white powder
Solubility	Soluble in water
Ph Range For Buffering	6.8 - 8.2
Pka	7.55 (at 25°C)
Chemical Stability	Stable under normal conditions
Osmolarity	Low osmotic effect when used in appropriate concentrations
Biocompatibility	Good biocompatibility

Packing & Storage

Packing	100g of Hepes - Na in sealed, chemical - resistant packaging for safe storage.
Storage	Store Hepes - Na (N-(2 - Hydroxyethyl)Piperazine - N'-2 - Ethanesulfonic Acid, Sodium Salt) in a cool, dry place. Keep it away from sources of heat and ignition, as excessive heat can degrade the chemical. Store in a tightly - sealed container to prevent moisture absorption, which could impact its purity and chemical properties.
Shipping	Hepes - Na will be carefully packaged to prevent damage. Shipped in well - sealed containers suitable for chemicals, ensuring safety during transit. Delivery options may vary, with tracking provided for customer convenience.

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Hepes-Na-N-(2-Hydroxyethyl)Piperazine-N'-2-Ethanesulfonic Acid,Sodium Salt

General Information

Historical Development

The industry of chemical industry is changing with each passing day, and all kinds of products have their own origins. Hepes - Na - N - (2 - Hydroxyethyl) Piperazine - N '- 2 - Ethanesulfonic Acid, Sodium Salt This product was originally developed to solve biochemical problems. In the past, scholars have studied and experienced many hardships to achieve this result. Its birth was not achieved overnight, but was the result of years of exploration. From initial conception to repeated trials, every step was dedicated. Over time, this product has gradually developed its capabilities in the field of biochemistry, and its application has become more and more extensive. It has paved the foundation for scientific research, promoted biochemical research and witnessed the evolution of chemical history.

Product Overview

Hepes-Na, that is, N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt, is a delicate reagent used in the analysis of pharmaceuticals. Its mild nature, in the biochemical reaction system, can stabilize and control the pH, just like the needle of the sea, to protect the smooth reaction.
Looking at its characteristics, it dissolves well and can be easily soluble in water and various organic solvents, which is convenient for the preparation of various experiments. And its buffering performance is excellent, which can maintain the balance of acid and base in the system in a wide pH range.
In the fields of biomedicine and molecular biology, this product is widely used. When cell culture, it can create a suitable acid-base environment for cells to help cells thrive; in the study of nucleic acid and protein, it also relies on its stable system to ensure the accuracy of experiments. It is a good companion for scientific research and is indispensable.

Physical & Chemical Properties

Hepes-Na, that is, N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt, this compound has unique physical and chemical properties. It is a white crystalline powder that is highly soluble in water to form a clear solution. Chemically, it is an excellent biological buffer that can effectively maintain a specific pH range and is widely used in biochemical and cell biology experiments. In terms of physical properties, the melting point is quite stable, making it easy to store and use. Due to its excellent stability and low interference with biological systems, it is indispensable in many experimental scenarios. Whether it is cell culture or enzyme activity research, it can rely on its stability characteristics to ensure a suitable experimental environment and help scientific research. It is like a solid foundation in the scientific research journey.

Technical Specifications & Labeling

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid. Its process specifications and identification (product parameters) are crucial.
Looking at its process, from the selection of raw materials, it is necessary to choose pure and high-quality products to ensure product purity. The preparation process is rigorous and orderly, and the reaction is based on specific proportions and processes to ensure accurate ingredients.
In terms of identification, the product parameters are clear. Its purity should reach a very high standard, and the impurity content should be minimal. The characters should be stable, and the color and texture should be uniform. On the packaging, key information such as name, composition, purity, storage conditions, etc. are clearly labeled to make it clear to the user at a glance, in order to meet the needs of experiments and production, and to meet the needs of scientific research and industry.

Preparation Method

The method of preparing Hepes-Na (N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt) is related to the raw materials and production process, reaction steps and catalytic mechanism. Take an appropriate amount of N- (2-hydroxyethyl) piperazine and the raw material containing the sulfonic acid group in a specific reaction vessel, and put it in a precise ratio. Adjust the reaction temperature to a suitable range, add a specific catalyst to promote its full reaction. During this period, closely observe the reaction process and control the reaction rate. After the reaction is completed, the impurities are removed through a series of separation and purification methods, and the pure Hepes-Na product is obtained. In this way, high-quality Hepes-Na products can be prepared to meet the needs of all parties.

Chemical Reactions & Modifications

Hepes-Na, that is, the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, is also essential in chemical reactions. This compound is often used as a chemical compound to control the acidity of the reaction environment. Its ability to maintain the stability of the phase, so that the reaction can be beneficial.
Its chemical properties, Hepes-Na has good performance, can effectively resist the influence of external factors of acidity. It is indispensable in both biochemical and industrial processes. Because it can change the activity of the reaction material, the rate of the reaction, and improve the rate of the reaction product.
Therefore, Hepes-Na plays an important role in chemical research and phase engineering in terms of its effectiveness in improving chemical performance.

Synonyms & Product Names

Hepes-Na, that is, N- (2-hydroxyethyl) piperazine-N '- 2-ethanesulfonate sodium salt, is widely used in biochemical research. Its nicknames are also popular, such as hydroxyethyl piperazine ethanesulfonate sodium, etc. The various trade names in Guanfu City are also different.
Because Hepes-Na has good buffering properties and can stabilize the pH of the solution, it is indispensable in cell culture, enzymatic reactions and other experiments. In the past, biochemical research was just emerging, and many sages worked hard on the selection of buffers. After years of research, trial and error, they got Hepes-Na and other good products.
The reason is that, although their names vary, their essence is to assist biochemical exploration, make research smooth, and produce frequent results, which contribute greatly to the development of the biochemical field.

Safety & Operational Standards

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonate. The safety and operation specifications of this chemical are of paramount importance.
Anyone who handles this substance must read the relevant books carefully to understand its properties and methods. When taking it, when it is in a purifier, do not let sundries into it, causing it to be stained. And it needs to be operated in a clean and well-ventilated place to avoid fireworks and heat sources to prevent accidents.
When storing, it should be placed in a dry and cool place, away from strong acids, bases and oxidized substances, and sealed in a container to prevent it from being damp and deteriorated. If you come into contact with this object on the skin, rinse it with water as soon as possible. If it enters your eyes, you need to rinse it with a lot of water urgently and seek medical attention.
When using a finished utensil, wash it according to regulations to prevent residue from remaining. Sprinkling objects should be collected quickly in a special utensil and handled in accordance with environmental protection regulations. They should not be disposed of at will.
In order to ensure safety, those who use this object should be familiar with emergency methods and always have medicines for treatment. In this way, they can use it and avoid its harm, so as to achieve scientific research and protect personal safety.

Application Area

Hepes-Na, that is, N- (2-hydroxyethyl) piperazine-N '- 2-ethanesulfonate sodium salt, has a wide range of application fields. In the context of biochemical research, it is often used as a buffer, which can stabilize and control the pH value of the reaction system and maintain the anterograde biochemical reaction. In cell culture, it can create an acid-base environment suitable for cell growth and promote healthy cell proliferation. In drug development, it helps drugs maintain stable chemical properties and improve drug efficacy. This substance plays a key role in many scientific research and practical application scenarios. It is an indispensable material for researchers to carry out related research, and helps to explore the mysteries of life and promote medical progress.

Research & Development

In recent years, I have been in the field of biochemistry, exploring Hepes - Na - N - (2 - Hydroxyethyl) Piperazine - N '- 2 - Ethanesulfonic Acid, Sodium Salt. Its properties are different. In the context of biochemical reactions, it often acts as a buffer agent, stabilizes the acid and base of the solution, and protects the properties of all biochemical substances.
Begin to explore its system. After all kinds of attempts, adjust all kinds of parameters, and hope to obtain pure and excellent products. The temperature, time, and ratio of agents of the reaction are all carefully studied. At the beginning, the fruit is not as expected, the quality is not pure, and the rate is not good. However, he was not discouraged, he repeatedly adjusted the strategy, and finally obtained the best method, and the product was excellent and the rate rose.
Re-research its use. For the cultivation of cells, add this agent, the state of cells is good, and the colonization is smooth. In the analysis of enzymes, stabilize their rings, and the nature of enzymes is constant. From this perspective, this product has great function in the research of biochemistry. I will cultivate it deeply, and promote its wider use in various domains, for the advancement of biochemistry, and exhaust my wisdom and strength.

Toxicity Research

I am committed to the toxicity research of chemicals, and recently focused on Hepes - Na - N - (2 - Hydroxyethyl) Piperazine - N '- 2 - Ethanesulfonic Acid, Sodium Salt. It has attracted much attention in many chemical experiments and industrial applications, but the toxicity research is still lacking.
I adhere to a cautious attitude and explore its toxicity with a scientific method. Observe its properties, observe its response to other substances, and analyze its changes in different environments. Hope to know its impact on biological organisms in detail and understand its potential danger. It is hoped that this research can establish safety regulations for applications in related fields, avoid harm caused by unknown toxicity, and ensure the safety of people and the environment. Make this substance under reasonable control, make the best use without harm, and seek well-being for the advancement of chemistry and industry.

Future Prospects

Guanfu Hepes - Na - N - (2 - Hydroxyethyl) Piperazine - N '- 2 - Ethanesulfonic Acid, Sodium Salt is really an exquisite chemical product. Although it has certain capabilities now, it still has a broad environment in the future.
In the future, it may be able to reveal a more brilliant appearance in the field of biomedicine. Help precision medicine, open up a new path for disease healing. In the realm of cell culture, it can become a stable cornerstone, ensuring safe cell growth and outstanding performance. And it is expected to be in the path of scientific research exploration, such as sharp blades breaking barriers, adding strong power to the solution of mysteries. Make our understanding of the microscopic world deeper. Expect this product to shine in the future, benefit many fields, and achieve extraordinary things.

Frequently Asked Questions

What is the chemical structure of Hepes - Na - N- (2 - Hydroxyethyl) Piperazine - N '-2 - Ethanesulfonic Acid, Sodium Salt?

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonate, and its chemical structure is as follows.
The main structure of this compound is a piperazine ring. A 2-hydroxyethyl group is connected to the nitrogen atom at one end of the piperazine ring, and a 2-ethanesulfonate group is connected to the nitrogen atom at the other end, and the sulfonate group exists in the form of sodium salt. From the structural point of view, the piperazine ring is a six-membered nitrogen-containing heterocycle, which has certain rigidity and chemical stability. The presence of 2-hydroxyethyl gives the molecule a certain hydrophilicity, because its hydroxyl group can participate in the formation of hydrogen bonds and enhance the solubility in water. 2-Ethane sulfonate is a strong acidic group, and its sodium salt form can be completely ionized in aqueous solution, which makes the compound have good buffering ability and is often used in biological buffer systems. The overall structure characteristics enable it to effectively maintain the pH stability of the system within a specific pH range, and it is widely used in biochemistry and cell biology experiments to ensure that the activity and function of biological macromolecules and cells are not affected by violent changes in pH.

What are the common applications of Hepes - Na - N- (2 - Hydroxyethyl) Piperazine - N '-2 - Ethanesulfonic Acid, Sodium Salt in biological experiments?

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, which is widely used in biological experiments.
First, it is often seen in the field of cell culture. Cell culture requires accurate maintenance of a stable pH environment. Hepes-Na can effectively resist pH fluctuations caused by external factors by its excellent buffering ability, creating a stable growth microenvironment for cells. When cells are cultured in vitro, they are highly susceptible to changes in medium composition and accumulation of cell metabolites. Hepes-Na can maintain pH in an appropriate range to ensure normal physiological function and growth and reproduction of cells.
Second, protein-related experiments are also commonly used. The structure and function of proteins are extremely sensitive to environmental pH. In experiments such as protein separation, purification and activity research, the Hepes-Na buffer system can stabilize the pH of the solution, prevent the protein from denaturing due to pH discomfort, ensure that the protein is in its natural active state, and help the experiment to accurately explore the characteristics and functions of proteins.
Third, in enzymatic reaction experiments, Hepes-Na also plays a key role. The catalytic activity of enzymes depends on specific pH conditions. Hepes-Na buffer can provide and maintain this suitable pH, so that the enzyme can fully demonstrate the catalytic efficiency and ensure the smooth progress of enzymatic reactions, which is of great significance for the study of enzyme kinetics and enzyme mechanism.
Fourth, there are also many applications in nucleic acid research. In DNA and RNA extraction, amplification and related analysis experiments, it is indispensable to stabilize the pH environment. Hepes-Na can effectively maintain the pH stability of the system, avoid the damage of nucleic acid structure, and ensure the accuracy and reliability of nucleic acid experiment results.

What are the storage conditions of Hepes - Na - N- (2 - Hydroxyethyl) Piperazine - N '-2 - Ethanesulfonic Acid, Sodium Salt?

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid. This substance should be stored in a cool and dry place, protected from direct light and moisture. Due to its chemical properties, if exposed to high temperature or humid environment, it may cause changes in its properties and affect its quality and utility.
When stored, it should be placed in a sealed container to prevent reaction in contact with air. Common storage temperature is about 2-8 ° C. In this temperature range, its stability is quite good. If the temperature is too high, the molecular activity will be enhanced, or adverse reactions such as decomposition will be triggered; if the temperature is too low, it may cause crystallization, which will also affect the use.
In addition, the storage place should be kept away from oxidants, acids, bases and other substances. Due to the specific groups in the chemical structure, it is easy to chemically react with these substances and cause deterioration. In the laboratory, it is often placed in a special reagent cabinet and arranged in an orderly manner according to the type of chemical substances, which is convenient for access and avoids mutual interference, so as to ensure that Hepes-Na maintains good performance during storage.

What are the advantages of Hepes - Na - N- (2 - Hydroxyethyl) Piperazine - N '-2 - Ethanesulfonic Acid, Sodium Salt over other buffers?

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid. Compared with other buffers, its advantages are as follows:
First, Hepes-Na has good buffering ability. In a wide pH range, especially near the physiological pH (pH 6.8-8.2), it can effectively maintain the stability of the pH of the system. Just like a rock-solid dike, it resists the impact of external acid-base changes on the pH of the system and provides a stable environment for many biochemical reactions. In the field of cell culture, cells are extremely sensitive to pH. Hepes-Na can accurately maintain the pH of the culture medium and ensure the normal growth and metabolism of cells.
Second, this buffer is chemically stable. It is not easy to chemically react with other substances in the system, just like a gentleman who is clean and protected. Under extreme conditions such as high temperature and high ionic strength, it can also maintain its own properties unchanged, so it is suitable for a variety of experimental conditions. For example, in some biochemical experiments that require high temperature treatment, Hepes-Na will not decompose or deteriorate due to rising temperature, ensuring the smooth progress of the experiment.
Third, Hepes-Na has good biocompatibility. It is almost non-toxic and disturbing to biological cells and tissues. It is like a gentle breeze and gently cares for biological systems. In biomedical research, experiments involving biological active substances such as cells and enzymes do not adversely affect their activity, thus ensuring the accuracy and reliability of the experimental results.
Fourth, good solubility. Whether in water or some organic solvents, it can quickly dissolve to form a uniform solution, like ice and snow in the warm sun, quickly integrated into it. This makes it easy to prepare different concentrations of buffers during experimental operations, providing convenience for experiments.

What is the pH buffer range of Hepes - Na - N- (2 - Hydroxyethyl) Piperazine - N '-2 - Ethanesulfonic Acid, Sodium Salt?

Hepes-Na is the sodium salt of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, which is a commonly used biological buffer. Its pH buffer range is about 6.8 to 8.2. In this range, Hepes-Na can effectively resist the large pH change caused by the addition of a small amount of acid or base, and maintain the relative stability of the pH of the system.
This buffer range makes Hepes-Na widely used in many biochemical and cell biology experiments. When cell culture, cells are suitable for growing in an environment close to the physiological pH value, and Hepes-Na can create a stable pH condition to ensure normal cell physiological activities and metabolism. In enzymatic reactions, most enzymes also need a specific pH range to exhibit the best activity. This buffer can ensure the stability of the pH of the reaction system and enable the enzyme to perform its catalytic function efficiently.
In short, Hepes-Na plays a key role in maintaining the pH stability of biological systems due to the specific pH buffer range, providing a solid foundation for the smooth development of various biological experiments.