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Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid

    Specifications

    HS Code

    992713

    Name Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid
    Chemical Formula C8H18N2O4S
    Molar Mass 238.305 g/mol
    Appearance White to off - white crystalline powder
    Ph Range For Buffering 7.2 - 8.8
    Pka At 25 C 7.55
    Solubility Soluble in water
    Melting Point 150 - 152°C
    Stability Stable under normal conditions
    Application Biochemical research, buffer in biological systems

    As an accredited Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid factory, we enforce strict quality protocols—every batch undergoes rigorous testing to ensure consistent efficacy and safety standards.

    Packing & Storage
    Packing 500g of Hepps in a sealed, chemical - resistant plastic bag for safe storage.
    Storage HEPES (N-(2-Hydroxyethyl)Piperazine-N'-3-Propanesulfonic Acid) should be stored in a cool, dry place, away from heat sources and direct sunlight. Keep it in a tightly sealed container to prevent moisture absorption and contamination. Ideal storage conditions are at room temperature, around 15 - 30°C, though some formulations may specify more precise ranges for optimal long - term stability.
    Shipping Hepps (N-(2-Hydroxyethyl)Piperazine-N'-3-Propanesulfonic Acid) is shipped in carefully sealed containers, safeguarded against moisture and physical damage. Shipment follows strict chemical transport safety regulations.
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    Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid
    General Information
    Historical Development
    Hepps, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid is also. The beginning of its application originated from the research of various scholars. In the past, scholars were diligent in the field of biochemistry, wanting to obtain the best agent suitable for biochemical experiments. After years of hard work, Hepps has appeared. At first, or only known to the general public, its performance is outstanding, it is effective in buffer systems, and it can stabilize pH. Gradually used by all researchers, the field of application has also become wider, from cell culture to protein research, all have seen its shadow. Over the years, Hepps, with its reliability, has moved forward steadily on the road of biochemical research, assisting scientific research and becoming an indispensable agent.
    Product Overview
    Hepps are N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. This compound is mild in nature and has many applications in biochemical research. It can maintain the stability of acid and base in solution and provide a suitable environment for many biochemical reactions.
    Looking at its characteristics, it has good water solubility and can be balanced in a wide temperature range of retinoic acid and alkali. And it is less disturbed by biomolecules, so as not to disturb its properties and structure. It is indispensable in the research of proteins and enzymes, and in the environment of cell culture.
    When preparing, it is necessary to follow fine methods to control temperature and quality to obtain pure products. The quality of its quality is related to the accuracy of experiments and the progress of scientific research. Therefore, those who study this thing must be careful, observe its nature, and make good use of it, which will add to the study of biochemistry.
    Physical & Chemical Properties
    Hepps, namely N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, has important physicochemical properties. This substance is in the form of a white crystalline powder and has stable properties. The melting point is between 234-238 ° C, and it has good solubility in water at room temperature, and can form a clear solution. Its pH buffer range is about 7.2-8.5, which can effectively maintain the pH stability of the system in this range. Chemically, it has amphoteric properties and can react moderately with acids and bases. Due to its special physicochemical properties, it is widely used in biochemical experiments, drug research and development and other fields. It can create a suitable pH environment for the reaction and ensure the smooth progress of experiments or production.
    Technical Specifications & Labeling
    Hepps, that is, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. The technical specifications and labels of its products (commodity parameters) are the key. Hepps need to have a specific purity, and the impurity content must be strictly controlled. Looking at its properties, it should be a white crystalline powder with good solubility. On the label, its chemical name, molecular formula, and molecular weight must be stated. The scope of application is indicated. For example, it is a genus of biological buffers, which are widely used in biochemical experiments. Its stability also needs to be marked, and its quality can only be guaranteed under suitable conditions. This is also specified in the technical specifications and labels of Hepps products (commodity parameters).
    Preparation Method
    The first step in making Hepps is to choose good raw materials. Take 2-hydroxyethylpiperazine and 3-propanesulfonic acid as the base materials, and the two must be pure and free of impurities. The preparation process involves first placing an appropriate amount of 2-hydroxyethylpiperazine in a clean reactor, controlling the temperature at a suitable temperature, slowly adding 3-propanesulfonic acid, and stirring continuously during the period to ensure that the two are fully mixed.
    During the reaction process, closely monitor the changes in temperature and pressure, and adjust them in sequence. After the reaction is completed, cool down, and remove impurities with a special filter device to obtain crude products. After
    , the crude product is refined, dissolved in a suitable solvent, recrystallized many times to remove its impurities and improve its purity. Finally, after drying treatment, high-purity Hepps products are obtained. The production mechanism is rigorous, and each step is interlocked to obtain a good product to meet the needs of all parties.
    Chemical Reactions & Modifications
    To taste the wonders of chemical industry, it is related to the change of substances, and reaction and modification are particularly critical. Today there are Hepps, that is, N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid. In the field of chemical industry, the reaction is particularly important.
    Looking at the reaction, the conditions are changeable, and the temperature and humidity and the genus of the catalyst are affected. At a suitable temperature, the reaction is orderly. If the temperature is high or low, the reaction can be deviated. The choice of catalyst is also related to the rate and effectiveness.
    On its modification, it is designed to optimize its properties. Or increase its stability, so that it can exist in different environments; or change its solubility to meet the needs of variety. After fine regulation of the reaction and rational modification, Hepps performance can be optimized, and it is widely used in the chemical industry.
    Synonyms & Product Names
    Hepps, also known as N- (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, has a wide range of uses in the field of chemical preparations. It seems to be a commonly used buffer in biochemical research. In a specific experimental environment, it can stabilize the pH of the solution and maintain the stability of the reaction system.
    Looking at its various nicknames, they are all used by the academic community for convenience. Or because of the difference in structure and activity, or because of the convenience of application, the same substance has different names. This is also like the ancient things, which change with the world, the place is different, and the names are also different.
    Hepps is like a sea needle in the scene of biochemical experiments, protecting the smooth reaction and ensuring the accuracy of the result. Due to its characteristics, it is indispensable in many fields such as cell culture and enzymatic reactions. It is also an ancient tool that helps craftsmen achieve success.
    Safety & Operational Standards
    Hepps, that is, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. In the research of chemical drugs, its safety and operating standards are of paramount importance.
    All the handling of Hepps involves the adaptation of the environment. When it is used in a well-ventilated place, keep its dust from rising in the air to prevent entry into the body and damage the respiratory system. And it must be kept away from fire and heat sources to prevent the danger of open flames, which may be flammable.
    As for the person who handles it, he must wear appropriate protective equipment. Wear goggles to protect your eyes from damage; wear protective clothing to protect your body and skin; wear protective gloves to prevent the danger of hand contact.
    When taking Hepps, the action should be slow and careful, and do not spill. If there is a spill, clean it up immediately. First collect it with a clean device, and then remove the residue with an appropriate solvent, leaving no hidden dangers.
    When storing, there are also rules. It should be placed in a cool, dry and ventilated place, away from direct sunlight, and stored separately from oxidizers, acids, etc., to avoid their reaction and risk safety.
    Furthermore, in the experimental or production process of using Hepps, complete emergency measures should be set up. If someone touches it, sucks it or takes it by mistake, the corresponding rescue measures should be immediately implemented. Those who touch the skin should be flushed with a large amount of water as soon as possible; those who enter the eye should be urgently rinsed with flowing water or normal saline and seek medical treatment; those who inhale should be moved to a fresh air place and given artificial respiration if necessary; those who take it by mistake should drink an appropriate amount of warm water, induce vomiting and seek medical attention.
    In this way, only in the research and development of Hepps can we abide by safety regulations, follow the scope of operation, ensure the smooth progress of research, and ensure the well-being of personnel.
    Application Area
    Hepps, that is, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. It has a wide range of uses in biochemical experiments. In the context of cell culture, it can stabilize the acid and alkali of the culture medium, so that the cells can be in a suitable environment and help them grow and multiply. In protein research, it can maintain the structure and activity of the protein without denaturation. In the enzymatic reaction, it provides stable pH conditions, so that the enzyme effect can be developed. This substance is an indispensable reagent in biochemistry, cell biology and other fields, helping researchers explore the mysteries of life, uncover the truth of biological processes, and contribute to scientific progress.
    Research & Development
    In the field of Wuyan Chemical Industry, Hepps is N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. Its properties are unique, and it is crucial in biochemical experiments, pharmaceutical research and development.
    At the beginning, analyze its physicochemical properties, and explore its stability and reactivity in different environments. Try various processes to optimize the synthesis method, hoping to increase its yield and reduce its impurities.
    After long-term research, the synthesis technique has gradually refined, and the purity of the product has also increased. However, it is still not enough, and I want to expand its application domain. Looking at the field of medicine, think about its potential in drug carriers and buffer systems; observe biochemical experiments, and hope that it can help with accurate analysis and efficient reactions.
    At present, although the research of Hepps has made progress, the road ahead is still far away. Our generation should continue to study, seek its wider application, higher quality, and contribute to the development of chemical industry.
    Toxicity Research
    The industry of chemical industry is related to people's livelihood, and new products emerge one after another, but the study of its toxicity cannot be ignored. Today there are Hepps, that is, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, which has emerged in various chemical products.
    To study the toxicity of Hepps, we have conducted various experiments. Take white mice as a test, feed them a diet containing Hepps, and observe their daily activities, diet and daily life. Cells were also taken and placed in a petri dish containing Hepps to observe the growth and differentiation of cells.
    After months of research, the results of the test were obtained. In the test of white mice, no significant abnormalities were found, and their diet and activities were normal, and they were healthy. Cell tests also showed that Hepps did not significantly hinder cell growth and differentiation. From this point of view, Hepps is quite toxic, and it is a safe choice for many applications. However, it still needs constant attention and cannot be slack. It is hoped to add a reliable material for the chemical industry and people's livelihood.
    Future Prospects
    Guanfu Hepps This substance, namely N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, has a wide range of uses in the field of biochemistry. Although it has its use today, it still has broad prospects for the future.
    Its stability and good solubility, in biochemical experiments, often used as a buffer agent to keep the environment stable and help the reaction smooth. In the future, science and technology will be new, or it can be used in the context of medical treatment, and there will be new functions. Disease diagnosis may be more accurate because of it; drug development may be used to increase efficiency.
    It is also expected to be used in bioengineering to expand its capabilities. Cell culture, with this help, may be able to breed excellent species and increase yield. In the future, if Xinghan is brilliant, Hepps will also shine brightly, contributing to the development of biochemistry and creating infinite possibilities.
    Where to Buy Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid in China?
    As a trusted Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid manufacturer, we deliver: Factory-Direct Value: Competitive pricing with no middleman markups, tailored for bulk orders and project-scale requirements. Technical Excellence: Precision-engineered solutions backed by R&D expertise, from formulation to end-to-end delivery. Whether you need industrial-grade quantities or specialized customizations, our team ensures reliability at every stage—from initial specification to post-delivery support.
    Frequently Asked Questions

    As a leading Hepps N-(2-Hydroxyethyl)Piperazine-N-3-Propanesulfonic Acid supplier, we deliver high-quality products across diverse grades to meet evolving needs, empowering global customers with safe, efficient, and compliant chemical solutions.

    What are the main uses of Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid?
    Hepps, or (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, has a wide range of uses in biochemical experiments.
    First, in the field of cell culture, Hepps is the key. Cell growth and reproduction require a precise acid-base environment. Hepps has excellent buffering properties, which can stabilize the pH value of cell culture medium within a specific range. In this way, cells can grow in a stable chemical environment, and their physiological functions and metabolic activities can proceed normally. If the pH value of the culture medium fluctuates too much, it may cause cell damage, growth inhibition, and even apoptosis. Therefore, Hepps is of great significance in maintaining the acid-base homeostasis of cell culture medium.
    Second, in the field of protein research, Hepps is also indispensable. The structure and function of proteins are extremely sensitive to the pH value of the environment. In the experimental process of protein extraction, purification and activity determination, the stable pH environment created by Hepps can protect the natural structure and biological activity of the protein. Otherwise, improper pH value may denature the protein and lose its original function, which will deviate the experimental results.
    Third, in the enzymatic reaction experiment, Hepps plays a significant role. Enzymes are biological catalysts, and their activity is closely related to the pH value of the reaction environment. Hepps can provide suitable pH conditions for enzymatic reaction, so that the enzyme can exert the best catalytic efficiency. Different enzymes have different optimum pH values. The buffering effect of Hepps can precisely regulate the pH value of the reaction system according to the characteristics of the enzyme to ensure the efficient and orderly progress of the enzymatic reaction.
    What are the physical and chemical properties of Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid
    Hepps, that is, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, is a commonly used zwitterion buffer in biochemical research. Its physical and chemical properties are quite characteristic.
    Looking at its properties, it is often in the shape of white crystalline powder, which is easy to weigh and take, and is quite convenient for experimental operation.
    When it comes to solubility, Hepps is easily soluble in water, which makes it quickly and uniformly dispersed in aqueous solution, which is conducive to the construction of a stable buffer system. At different temperatures, its solubility varies slightly, and when the temperature increases, the solubility also increases slightly. Usually at 25 ° C, it can dissolve about [X] grams of Hepps per 100 ml of water.
    In terms of pH, the pKa value of Hepps is about 7.5-8.5, which makes it play a good buffering role in the near-neutral physiological pH range, which can effectively maintain the stability of the pH value of the solution and resist the pH fluctuation caused by the addition of a small amount of acid or base.
    The chemical stability of Hepps is also good, and it is not easy to decompose at room temperature and pressure, and can be stored for a long time. However, it should be noted that it should be avoided from contact with strong oxidants, strong acids, strong bases and other substances to prevent chemical reactions from occurring and affecting its buffering performance. In addition, Hepps has a certain tolerance to most metal ions, and can still maintain the stability of the buffer system in the presence of common metal ions, which provides convenience for many biochemical experiments involving metal ions.
    What are the advantages of Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid in biological experiments
    Hepps, that is (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, is very beneficial in the application of biological experiments.
    First, this substance has good buffering properties. In biological systems, it can effectively maintain the stability of pH. The cover biological reaction is extremely sensitive to pH, small changes or reaction disorders, and even failure. Hepps can resist acid-base changes in a specific pH range, stabilize the reaction environment, and ensure the smooth progress of biological experiments.
    Second, Hepps has good biocompatibility. In experiments such as cell culture, it interacts gently with biomolecules, cells, etc., without interfering with the normal function of biomolecules, nor does it damage the activity and morphology of cells, which can accurately reflect the original state and characteristics of biological systems.
    Third, its solubility is quite good. Whether it is an aqueous system or some organic solvents, it can be well dissolved, which is convenient for experimenters to flexibly choose suitable solvents to prepare reaction systems according to different experimental needs and broaden the scope of application.
    Fourth, strong stability. Under common experimental conditions, it is not easily decomposed or deteriorated by factors such as temperature, light, oxidation, etc., to ensure the repeatability and reliability of experimental results, so that experiments carried out at different times and places can achieve similar results.
    In summary, Hepps provides solid support for biological research with its advantages in buffering performance, biocompatibility, solubility and stability in biological experiments, helping researchers to deeply explore the mysteries of life.
    How Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid is Stored
    Hepps is (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. This substance should be properly stored to ensure its stability and purity. When stored, choose a suitable place according to its physical and chemical properties.
    First weight moisture-proof. Hepps are hygroscopic. If exposed to moisture, it is easy to absorb moisture and cause changes in properties and purity. Therefore, when stored in a dry place, a sealed container can be used to avoid water vapor from entering.
    Second time is temperature control. If the temperature is too high or fluctuates, it may affect its chemical structure and stability. It should be placed in a cool place, preferably at 15-25 ° C, to prevent direct sunlight and close to heat sources.
    In addition, avoid mixing with sundries. Hepps should be left alone, and should not coexist with oxidation, reduction, acid and alkali substances, so as to avoid chemical reactions and deterioration.
    In the place of storage, when clearly marked, remember its name, sex, storage method and pay attention to all things, it will be taken and managed. Check its status regularly to see if there is deliquescence, discoloration, and agglomeration. If there is any abnormality, deal with it quickly to ensure its availability. In this way, Hepps can keep its quality for a long time for experimentation and production needs.
    What is the price range for Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid?
    The price range of Hepps, which is (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, fluctuates due to a variety of factors. In terms of the ancient text of "Tiangong Kaiwu", this chemical reagent is not inherent in the book, but it can be described as follows.
    Its price is restricted by many factors. The first is purity. If the purity is high, such as high-purity Hepps used in high-end scientific research, pharmaceuticals and other fields, the price will be high. High purity, less impurities, less interference with experiments or production, so the price is high. In terms of common purity grades, 99% purity is often higher than 95% purity.
    Furthermore, the manufacturer has a great influence. Well-known large factories, with their sophisticated craftsmanship and strict quality control, produce high-quality Hepps, and the price will be relatively high. And small factory products, although the price may be cheap, but the quality stability may be insufficient.
    The market supply and demand situation is also the key. If there are many scientific research projects at a time, the demand for Hepps increases sharply, and the supply is limited, the price will easily rise; on the contrary, if the supply exceeds the demand, the price will decline.
    In the market, the price of Hepps varies from a few yuan to tens of yuan per gram. Ordinary purity and small packaging are common in a few yuan per gram; if it is high purity and large-scale procurement, it may reach tens of yuan per gram. However, this is only an approximate amount. The actual price should be subject to the real-time market, and various factors are mixed, causing the price to fluctuate from time to time, and it should not be limited to a general price.