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What are the main uses of Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid?
Hepps, or (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, has a wide range of uses in biochemical experiments.
First, in the field of cell culture, Hepps is the key. Cell growth and reproduction require a precise acid-base environment. Hepps has excellent buffering properties, which can stabilize the pH value of cell culture medium within a specific range. In this way, cells can grow in a stable chemical environment, and their physiological functions and metabolic activities can proceed normally. If the pH value of the culture medium fluctuates too much, it may cause cell damage, growth inhibition, and even apoptosis. Therefore, Hepps is of great significance in maintaining the acid-base homeostasis of cell culture medium.
Second, in the field of protein research, Hepps is also indispensable. The structure and function of proteins are extremely sensitive to the pH value of the environment. In the experimental process of protein extraction, purification and activity determination, the stable pH environment created by Hepps can protect the natural structure and biological activity of the protein. Otherwise, improper pH value may denature the protein and lose its original function, which will deviate the experimental results.
Third, in the enzymatic reaction experiment, Hepps plays a significant role. Enzymes are biological catalysts, and their activity is closely related to the pH value of the reaction environment. Hepps can provide suitable pH conditions for enzymatic reaction, so that the enzyme can exert the best catalytic efficiency. Different enzymes have different optimum pH values. The buffering effect of Hepps can precisely regulate the pH value of the reaction system according to the characteristics of the enzyme to ensure the efficient and orderly progress of the enzymatic reaction.
What are the physical and chemical properties of Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid
Hepps, that is, N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, is a commonly used zwitterion buffer in biochemical research. Its physical and chemical properties are quite characteristic.
Looking at its properties, it is often in the shape of white crystalline powder, which is easy to weigh and take, and is quite convenient for experimental operation.
When it comes to solubility, Hepps is easily soluble in water, which makes it quickly and uniformly dispersed in aqueous solution, which is conducive to the construction of a stable buffer system. At different temperatures, its solubility varies slightly, and when the temperature increases, the solubility also increases slightly. Usually at 25 ° C, it can dissolve about [X] grams of Hepps per 100 ml of water.
In terms of pH, the pKa value of Hepps is about 7.5-8.5, which makes it play a good buffering role in the near-neutral physiological pH range, which can effectively maintain the stability of the pH value of the solution and resist the pH fluctuation caused by the addition of a small amount of acid or base.
The chemical stability of Hepps is also good, and it is not easy to decompose at room temperature and pressure, and can be stored for a long time. However, it should be noted that it should be avoided from contact with strong oxidants, strong acids, strong bases and other substances to prevent chemical reactions from occurring and affecting its buffering performance. In addition, Hepps has a certain tolerance to most metal ions, and can still maintain the stability of the buffer system in the presence of common metal ions, which provides convenience for many biochemical experiments involving metal ions.
What are the advantages of Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid in biological experiments
Hepps, that is (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, is very beneficial in the application of biological experiments.
First, this substance has good buffering properties. In biological systems, it can effectively maintain the stability of pH. The cover biological reaction is extremely sensitive to pH, small changes or reaction disorders, and even failure. Hepps can resist acid-base changes in a specific pH range, stabilize the reaction environment, and ensure the smooth progress of biological experiments.
Second, Hepps has good biocompatibility. In experiments such as cell culture, it interacts gently with biomolecules, cells, etc., without interfering with the normal function of biomolecules, nor does it damage the activity and morphology of cells, which can accurately reflect the original state and characteristics of biological systems.
Third, its solubility is quite good. Whether it is an aqueous system or some organic solvents, it can be well dissolved, which is convenient for experimenters to flexibly choose suitable solvents to prepare reaction systems according to different experimental needs and broaden the scope of application.
Fourth, strong stability. Under common experimental conditions, it is not easily decomposed or deteriorated by factors such as temperature, light, oxidation, etc., to ensure the repeatability and reliability of experimental results, so that experiments carried out at different times and places can achieve similar results.
In summary, Hepps provides solid support for biological research with its advantages in buffering performance, biocompatibility, solubility and stability in biological experiments, helping researchers to deeply explore the mysteries of life.
How Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid is Stored
Hepps is (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid. This substance should be properly stored to ensure its stability and purity. When stored, choose a suitable place according to its physical and chemical properties.
First weight moisture-proof. Hepps are hygroscopic. If exposed to moisture, it is easy to absorb moisture and cause changes in properties and purity. Therefore, when stored in a dry place, a sealed container can be used to avoid water vapor from entering.
Second time is temperature control. If the temperature is too high or fluctuates, it may affect its chemical structure and stability. It should be placed in a cool place, preferably at 15-25 ° C, to prevent direct sunlight and close to heat sources.
In addition, avoid mixing with sundries. Hepps should be left alone, and should not coexist with oxidation, reduction, acid and alkali substances, so as to avoid chemical reactions and deterioration.
In the place of storage, when clearly marked, remember its name, sex, storage method and pay attention to all things, it will be taken and managed. Check its status regularly to see if there is deliquescence, discoloration, and agglomeration. If there is any abnormality, deal with it quickly to ensure its availability. In this way, Hepps can keep its quality for a long time for experimentation and production needs.
What is the price range for Hepps N- (2-Hydroxyethyl) Piperazine-N-3-Propanesulfonic Acid?
The price range of Hepps, which is (2-hydroxyethyl) piperazine-N-3-propanesulfonic acid, fluctuates due to a variety of factors. In terms of the ancient text of "Tiangong Kaiwu", this chemical reagent is not inherent in the book, but it can be described as follows.
Its price is restricted by many factors. The first is purity. If the purity is high, such as high-purity Hepps used in high-end scientific research, pharmaceuticals and other fields, the price will be high. High purity, less impurities, less interference with experiments or production, so the price is high. In terms of common purity grades, 99% purity is often higher than 95% purity.
Furthermore, the manufacturer has a great influence. Well-known large factories, with their sophisticated craftsmanship and strict quality control, produce high-quality Hepps, and the price will be relatively high. And small factory products, although the price may be cheap, but the quality stability may be insufficient.
The market supply and demand situation is also the key. If there are many scientific research projects at a time, the demand for Hepps increases sharply, and the supply is limited, the price will easily rise; on the contrary, if the supply exceeds the demand, the price will decline.
In the market, the price of Hepps varies from a few yuan to tens of yuan per gram. Ordinary purity and small packaging are common in a few yuan per gram; if it is high purity and large-scale procurement, it may reach tens of yuan per gram. However, this is only an approximate amount. The actual price should be subject to the real-time market, and various factors are mixed, causing the price to fluctuate from time to time, and it should not be limited to a general price.