What are the main uses of Mes 2- (N-Morpholino) Ethanesulfonic Acid?
Mes, which is 2- (N -morpholino) ethanesulfonic acid, has a wide range of main uses and is of great significance in biochemical research and molecular biology experiments.
In biological buffer systems, Mes plays a key role. Because it has a suitable pKa value, it can effectively maintain the pH stability of the solution in a specific pH range. Many biochemical reactions are extremely sensitive to pH, and small changes may affect the process and results of the reaction. For enzymatic reactions, specific enzymes are only active in a suitable pH environment. The stable pH environment constructed by Mes can ensure the normal function of enzymes and help researchers accurately explore the mechanism of enzymatic reactions.
In the field of cell culture, Mes is also indispensable. Cell growth has strict environmental requirements and pH stability is essential. The addition of Mes medium can avoid large fluctuations in pH caused by cell metabolism, create a good growth microenvironment for cells, maintain normal physiological functions and growth and proliferation of cells, and is widely used in various cell culture experiments, from ordinary cell lines to stem cell culture.
Nucleic acid and protein-related experiments, Mes also plays an important role. For nucleic acid extraction and purification, it is necessary to ensure the stability and integrity of nucleic acid structure under specific pH conditions. Mes buffer can meet this demand and prevent nucleic acid degradation; protein electrophoresis experiments, the appropriate pH environment has a great impact on protein separation. The buffer system regulated by Mes can effectively separate proteins according to their own charge and molecular weight differences, which is convenient for subsequent analysis and identification.
What are the physicochemical properties of Mes 2- (N-Morpholino) Ethanesulfonic Acid
MES is 2- (N-morpholino) ethanesulfonic acid, which is a commonly used buffer in biochemical research. Its physicochemical properties are as follows:
Appearance is mostly white crystalline powder, stable properties, easy to store and use. In terms of solubility, it is easily soluble in water, which makes it convenient to configure buffer solutions of different concentrations to suit a variety of experimental needs.
The buffer range of MES is very critical, about pH 5.5 - 6.7. In this pH range, it can effectively resist the large change in pH caused by the addition of a small amount of acid or base, thus providing a stable acid-base environment for many biochemical reactions. For example, some enzymatic reactions are extremely sensitive to pH value, and MES buffer solution can ensure that the reaction proceeds smoothly under suitable pH conditions, maintaining the activity and stability of the enzyme.
From the perspective of the dissociation constant (pKa), its pKa value is about 6.1, which is closely related to the buffer range, which determines its effectiveness in buffering in a specific pH region.
In addition, the chemical properties of MES are relatively stable, and it is not easy to react with common chemicals under normal storage and use conditions. This can ensure that the buffer solution does not interfere with the target reaction in the experimental system and ensure the accuracy and reliability of the experimental results.
What should be paid attention to when storing Mes 2- (N-Morpholino) Ethanesulfonic Acid
Mes, that is 2- (N -morpholino) ethanesulfonic acid, this is a commonly used buffer in biochemical experiments. When storing, pay attention to many key points.
First storage temperature. It should be placed in a low temperature and stable place, usually 2-8 ° C. This is easy to change its chemical properties due to high temperature, which affects the buffering performance. If the temperature is too high, or the molecular structure changes, the buffering effect is greatly reduced.
The second is moisture-proof. Because of its hygroscopicity, if exposed to too much water vapor, the purity will decrease. Therefore, it should be stored in a dry place or packaged with moisture-proof packaging to prevent moisture deterioration.
Furthermore, it should be protected from light. Light or lead to luminescent chemical reactions, causing decomposition or structural changes. It should be stored in opaque containers or stored in dark places.
In addition, it should be placed separately from other chemicals. Because of its active chemical properties, it comes into contact with or reacts with certain substances. Avoid co-storage with oxidants, reducing agents, etc., to prevent failure or danger due to interaction.
At the same time, the storage period should not be ignored. It should be used in accordance with the valid period of the product description. Overdue or performance degradation. Regularly check the storage status. If there is any abnormality, such as discoloration or precipitation, do not use it again. In this way, the performance of Mes during storage is stable, and the experimental results are accurate and reliable.
What is the preparation method of Mes 2- (N-Morpholino) Ethanesulfonic Acid
The method for preparing MES (2- (N-morpholine) ethanesulfonic acid) is as follows:
First take an appropriate amount of morpholine and carefully place it in the reaction vessel. Then, at a suitable temperature and under stirring conditions, slowly add 2-chloroethanesulfonic acid dropwise. This process requires strict control of the reaction temperature to prevent side reactions.
After the dropwise addition is completed, continue to stir for a period of time to promote the full reaction of the two. After the reaction, the reaction solution is cooled and then extracted with a suitable organic solvent to remove impurities.
After extraction, the organic phase is collected and the organic solvent is removed by vacuum distillation to obtain crude MES.
To obtain high-purity MES, further refining is required. The crude product can be dissolved in an appropriate amount of water and decolorized by activated carbon to remove colored impurities. Then, the recrystallization operation is carried out. Select a suitable solvent, control the crystallization temperature and speed, and obtain pure MES crystals.
Finally, the crystals are filtered and dried to obtain the finished MES. During the preparation process, factors such as temperature, material ratio and reaction time have a great impact on the purity and yield of the product, and need to be carefully controlled.
What are the differences in the application of Mes 2- (N-Morpholino) Ethanesulfonic Acid in different fields
MES, which is 2- (N-morpholino) ethanesulfonic acid, is widely used in the fields of biochemistry and molecular biology, with different applications.
In the field of cell culture, MES is often used as a buffer. Cells are extremely sensitive to the pH value of the growth environment, and MES can maintain a stable pH range, generally between 6.0 and 7.0, which is suitable for most cell growth. Because of its good buffering capacity, it can resist pH fluctuations caused by cell metabolism to produce acid or alkali, create a stable microenvironment for cells, and ensure normal cell growth and metabolism. For example, in culturing some adherent cells that are sensitive to pH changes, the addition of MES buffer system can significantly improve cell survival and proliferation.
In the enzyme reaction system, MES plays a key role. Many enzymatic reactions have strict pH requirements, and MES buffers can precisely regulate pH on demand to ensure that enzymes are in the best active state. Different enzymes have specific optimal pH, such as some acid phosphatases with the strongest activity when the MES buffer pH is about 5.5, providing suitable conditions for related research and applications to ensure efficient and stable enzymatic reactions.
In nucleic acid electrophoresis experiments, MES is also indispensable. Nucleic acid electrophoresis requires a specific pH environment to ensure stable migration and separation of nucleic acid molecules. The MES buffer system can provide suitable pH and ionic strength, so that nucleic acid molecules can migrate orderly in the gel according to their own size and charge characteristics, so as to achieve accurate separation and analysis. For example, when separating small RNA fragments, the electrophoresis buffer containing MES can achieve ideal resolution and band clarity.