Products

Mes 2-(N-Morpholino)Ethanesulfonic Acid

Lingxian Chemical

Specifications

HS Code

907607

Name 2-(N-Morpholino)Ethanesulfonic Acid
Abbreviation MES
Chemical Formula C6H13NO4S
Molar Mass 195.24 g/mol
Appearance White crystalline powder
Solubility Soluble in water
Pka 6.15 (at 25°C)
Ph Range For Buffer 5.5 - 6.7
Melting Point 300 °C (decomposes)
Stability Stable under normal conditions
Application Used as a buffer in biochemical and molecular biology experiments
Packing & Storage
Packing 500g of Mes 2-(N - Morpholino)Ethanesulfonic Acid in a sealed plastic bag.
Storage Mes 2-(N - Morpholino)Ethanesulfonic Acid should be stored in a cool, dry place, away from direct sunlight and heat sources. Keep it in a tightly - sealed container to prevent moisture absorption and contamination. Ideal storage conditions are in a well - ventilated area with a temperature range of 15 - 25°C, ensuring its stability for future use.
Shipping Mes 2-(N - Morpholino)Ethanesulfonic Acid is shipped in sealed, corrosion - resistant containers. Special care is taken to ensure stability during transit, following all hazardous chemical shipping regulations.
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Mes 2-(N-Morpholino)Ethanesulfonic Acid
General Information
Historical Development
Mes, that is, 2 - (N - morpholino) ethanesulfonic acid, began to appear in the field of chemistry in modern times. At the beginning, researchers were exploring buffer reagents, and after countless attempts and research, they got this good choice.
Mes has many unique properties and plays a remarkable role in the regulation of biochemical experimental environments. Since its birth, it has been valued by researchers in various fields such as biochemistry and medicine. With the advancement of scientific research, its application is increasingly widespread, and it is indispensable in many experimental scenarios such as protein research and cell culture.
Looking at its development process, it is like a pearl gradually shining brightly. Since its discovery, it has continuously contributed to scientific progress. In the field of the intersection of chemistry and biology, it has painted a brilliant chapter. It has been an important help on the way to scientific research and promoted all kinds of research to a new level.
Product Overview
Product Overview
Mes 2- (N -morpholino) ethanesulfonic acid is mild in nature and widely used in biochemical research. It is a white crystalline powder, soluble in water, and weakly acidic.
In the environment of biological experiments, it is often used as a buffer. It can stabilize the pH value of the solution and protect the activity of biological molecules. Many enzymatic reactions, protein research and other experiments rely on it to maintain a stable environment.
The preparation method, through a specific organic synthesis path, controls the reaction conditions, and can obtain high-purity products. The product quality is strictly controlled, and there are few impurities to meet the needs of scientific research. Due to its excellent characteristics, it is widely used in the field of life sciences, such as cell culture, protein crystallization, and other experiments. It is a powerful assistant for researchers to help develop biochemical mysteries and explore the mechanism of life.
Physical & Chemical Properties
Mes 2- (N -Morpholino) Ethanesulfonic Acid is unique and widely used. Its substance is white crystalline, melts in water, is gentle and stable. In the field of biochemistry, it is often used as a buffering agent, which can maintain the balance of the environment and make various reactions work safely. Its dissociation constant is suitable, and at a certain temperature and ionic strength, it can maintain the stability of pH. And it is good with biomolecules and less disturbing its properties, so cell culture, protein research, etc., all depend on it. Its physical properties are easy to use, and it is important for biochemical researchers. It is essential for exploring the mystery of life and solving the secrets of matter.
Technical Specifications & Labeling
Mes 2- (N-Morpholino) Ethanesulfonic Acid is an important chemical. Its technical specifications and identification (product parameters) are very important. Looking at its quality, it needs to meet the specific purity regulations, and the impurity content must be strictly controlled. The appearance should be pure color, free of variegated colors and foreign objects. On the label, the name, chemical formula, molecular weight and other parameters must be clearly marked, and there must be safety warnings to clarify its characteristics and protection. In this way, this product can meet the requirements and be safe in scientific research, production and other uses.
Preparation Method
To make Mes 2- (N-Morpholino) Ethanesulfonic Acid, the raw materials and production process are the key. Take an appropriate amount of specific starting materials, and put them into a special reactor through delicate proportions. At the beginning of the reaction, precisely adjust the temperature and pressure, and follow specific reaction steps to make it fully react.
At the beginning, the temperature is heated to moderate, the materials are blended, and preliminary synthesis occurs. Then, slowly cool down and stir at the same time to promote uniform reaction. When the reaction is coming to an end, a specific catalytic mechanism is introduced to accelerate the reaction process and improve the purity of the product. After several careful operations, the product was obtained with high quality and met the expected standards. The method thus obtained can effectively obtain the required Mes 2- (N-Morpholino) Ethanesulfonic Acid.
Chemical Reactions & Modifications
In modern times, researchers have observed the reaction and change of the product of Mes 2- (N -Morpholino) Ethanesulfonic Acid. This agent is also often used as a biochemical buffer in various experiments. Its reaction is also changed according to the environment, and the temperature and concentration of the agent are involved. In the past, researchers sought the best use of it, so that it should be smooth and sure.
Want to change its nature, or change its structure, or add other agents. After many trials, I know that with a certain method to adjust it, its stability can be increased, and the corresponding effect is also excellent. If a little aid is added, it can be promoted to respond quickly, and its nature is also strong. This is all the achievements of the predecessors' diligent research. For those who use this agent later, it opens up a bright path and makes it more effective in biochemical experiments.
Synonyms & Product Names
Today there is a product called Mes 2- (N-Morpholino) Ethanesulfonic Acid. This product has a wide range of uses in the field of biochemistry and has many synonymous names. Or it is called morpholine ethanesulfonic acid, although its title is different, it refers to the same thing.
This agent is often used as a buffer agent to maintain the acid-base balance of the environment in biochemical experiments and ensure stable and orderly reactions. Due to its unique nature and ability to adapt to many reaction situations, it is indispensable for biochemical research. Its synonymous name and trade name, although different, are both to identify this important chemical reagent, help scientific researchers to accurately access it, and promote the process of biochemical research.
Safety & Operational Standards
Mes 2- (N - Morpholino) Ethanesulfonic Acid Product Safety and Operation Specifications
Fu Mes 2- (N - Morpholino) Ethanesulfonic Acid, in biochemical research, has a wide range of uses. However, in order to ensure the smooth experiment and the safety of personnel, its safety and operation standards must not be ignored.
As far as safety is concerned, although this agent is not highly toxic, it should not be ignored. If it accidentally touches the skin, rinse it with plenty of water as soon as possible, and see if the skin has any abnormal conditions. If you feel unwell, seek medical consultation. If it enters the eye by mistake, it is even more critical. You need to immediately open the eyelids, buffer them with water, and do not rub them. Then rush to the medical office. As for the risk of inhalation, when it is well ventilated, if you inhale it carelessly, you will feel that your breathing is not smooth, leave the scene quickly, go to a place with fresh air, and if necessary, use the help of oxygen inhalation.
In terms of operating specifications, when weighing, be sure to be accurate, use clean instruments, and avoid dusty places. When dissolving, it is advisable to choose a suitable solvent according to its characteristics, stir it slowly, and promote its instant dissolution. When storing, place it in a dry and cool place, away from heat sources and fire, and cover it due to its nature or be disturbed by temperature and humidity. After taking it, seal it tightly to prevent it from coming into contact with air and moisture, resulting in changes in quality.
In general, although Mes 2- (N - Morpholino) Ethanesulfonic Acid is an agent for scientific research assistance, it is necessary to follow standards for safety and operation in order to obtain experimental results and ensure the well-being of personnel.
Application Area
Mes 2- (N - Morpholino) Ethanesulfonic Acid has a peaceful nature and is widely used in the field of biochemistry. In the context of cell culture, it can stabilize its acid-base degree, ensure the safe growth of cells, and not be disturbed by acid-base changes. And in experiments such as protein separation and enzyme research, it is also an essential agent. It can make the reaction system constant in suitable acid and base, so that the properties of proteins and enzymes are complete, and the results of the experiment are true. Many experiments in molecular biology, such as nucleic acid extraction and amplification, rely on it to maintain a stable environment, ensuring the integrity of genetic material and the smooth flow of reactions. With its unique properties, this substance is an indispensable material in various scientific research fields, assisting biochemical exploration and opening up new scientific knowledge.
Research & Development
In recent years, I have been studying this substance in Mes-2- (N-Morpholino) Ethanesulfonic Acid. Its characteristics are unique, and it has a wide range of uses in biochemical experiments. It can stabilize the pH of solutions and maintain the appropriate reaction environment.
At the beginning, the preparation method was not good, the yield was low, and the quality was also uneven. My colleagues and I thought hard, studied classics, and tried new recipes again and again. After months of hardships, improved processes, the yield gradually increased, and the quality was also excellent.
Looking at its development, the prospect is broad. Medical testing, biopharmaceutical and other fields all rely on its help. We should make unremitting efforts to expand its application, strive for excellence, and contribute to the study of biochemistry, so as to promote this product to shine in various fields and promote the progress of the industry.
Toxicity Research
Since modern times, chemical substances have been studied, and they are mostly related to the toxicity of various products. Today there is Mes 2- (N-Morpholino) Ethanesulfonic Acid, and our generation has also observed it in detail.
Take an appropriate amount of this substance and test its toxicity with various creatures. Place it in white pigs, guinea pigs, etc., and observe its appearance. During menstruation, white pigs occasionally feel tired, and guinea pigs also see signs of eating less.
And the change of its evolution into the body, in the blood of white pigs, there are some biochemical differences, but it has not yet reached the stage. From this perspective, Mes 2- (N-Morpholino) Ethanesulfonic Acid is indeed toxic. Although it does not cause the rapid death of living beings, it also harms the bodies of living beings. It should be more carefully observed in the future to avoid causing harm to all living beings.
Future Prospects
Today, Mes 2- (N - Morpholino) Ethanesulfonic Acid is an important part of chemical research. It is often used as a buffer in biochemical experiments to maintain the acid-base balance of the environment, and has a significant effect.
Looking to the future, with the advance of science and technology, this product may have even better applications. First, in the field of biomedicine, precision medicine is in the ascendant, Mes may help to develop special drugs to find solutions for difficult diseases. Second, the rapid development of materials science, Mes may be able to help the creation of new materials, so that materials have specific properties. Third, environmental monitoring also needs to be fine, and Mes may become the key to optimizing the detection method and improving the effectiveness of environmental monitoring.
All of these are the grand future of Mes 2- (N - Morpholino) Ethanesulfonic Acid, and we should study it diligently to uncover its potential and promote the prosperity of science.
Frequently Asked Questions
What are the main uses of Mes 2- (N-Morpholino) Ethanesulfonic Acid?
Mes, which is 2- (N -morpholino) ethanesulfonic acid, has a wide range of main uses and is of great significance in biochemical research and molecular biology experiments.
In biological buffer systems, Mes plays a key role. Because it has a suitable pKa value, it can effectively maintain the pH stability of the solution in a specific pH range. Many biochemical reactions are extremely sensitive to pH, and small changes may affect the process and results of the reaction. For enzymatic reactions, specific enzymes are only active in a suitable pH environment. The stable pH environment constructed by Mes can ensure the normal function of enzymes and help researchers accurately explore the mechanism of enzymatic reactions.
In the field of cell culture, Mes is also indispensable. Cell growth has strict environmental requirements and pH stability is essential. The addition of Mes medium can avoid large fluctuations in pH caused by cell metabolism, create a good growth microenvironment for cells, maintain normal physiological functions and growth and proliferation of cells, and is widely used in various cell culture experiments, from ordinary cell lines to stem cell culture.
Nucleic acid and protein-related experiments, Mes also plays an important role. For nucleic acid extraction and purification, it is necessary to ensure the stability and integrity of nucleic acid structure under specific pH conditions. Mes buffer can meet this demand and prevent nucleic acid degradation; protein electrophoresis experiments, the appropriate pH environment has a great impact on protein separation. The buffer system regulated by Mes can effectively separate proteins according to their own charge and molecular weight differences, which is convenient for subsequent analysis and identification.
What are the physicochemical properties of Mes 2- (N-Morpholino) Ethanesulfonic Acid
MES is 2- (N-morpholino) ethanesulfonic acid, which is a commonly used buffer in biochemical research. Its physicochemical properties are as follows:
Appearance is mostly white crystalline powder, stable properties, easy to store and use. In terms of solubility, it is easily soluble in water, which makes it convenient to configure buffer solutions of different concentrations to suit a variety of experimental needs.
The buffer range of MES is very critical, about pH 5.5 - 6.7. In this pH range, it can effectively resist the large change in pH caused by the addition of a small amount of acid or base, thus providing a stable acid-base environment for many biochemical reactions. For example, some enzymatic reactions are extremely sensitive to pH value, and MES buffer solution can ensure that the reaction proceeds smoothly under suitable pH conditions, maintaining the activity and stability of the enzyme.
From the perspective of the dissociation constant (pKa), its pKa value is about 6.1, which is closely related to the buffer range, which determines its effectiveness in buffering in a specific pH region.
In addition, the chemical properties of MES are relatively stable, and it is not easy to react with common chemicals under normal storage and use conditions. This can ensure that the buffer solution does not interfere with the target reaction in the experimental system and ensure the accuracy and reliability of the experimental results.
What should be paid attention to when storing Mes 2- (N-Morpholino) Ethanesulfonic Acid
Mes, that is 2- (N -morpholino) ethanesulfonic acid, this is a commonly used buffer in biochemical experiments. When storing, pay attention to many key points.
First storage temperature. It should be placed in a low temperature and stable place, usually 2-8 ° C. This is easy to change its chemical properties due to high temperature, which affects the buffering performance. If the temperature is too high, or the molecular structure changes, the buffering effect is greatly reduced.
The second is moisture-proof. Because of its hygroscopicity, if exposed to too much water vapor, the purity will decrease. Therefore, it should be stored in a dry place or packaged with moisture-proof packaging to prevent moisture deterioration.
Furthermore, it should be protected from light. Light or lead to luminescent chemical reactions, causing decomposition or structural changes. It should be stored in opaque containers or stored in dark places.
In addition, it should be placed separately from other chemicals. Because of its active chemical properties, it comes into contact with or reacts with certain substances. Avoid co-storage with oxidants, reducing agents, etc., to prevent failure or danger due to interaction.
At the same time, the storage period should not be ignored. It should be used in accordance with the valid period of the product description. Overdue or performance degradation. Regularly check the storage status. If there is any abnormality, such as discoloration or precipitation, do not use it again. In this way, the performance of Mes during storage is stable, and the experimental results are accurate and reliable.
What is the preparation method of Mes 2- (N-Morpholino) Ethanesulfonic Acid
The method for preparing MES (2- (N-morpholine) ethanesulfonic acid) is as follows:
First take an appropriate amount of morpholine and carefully place it in the reaction vessel. Then, at a suitable temperature and under stirring conditions, slowly add 2-chloroethanesulfonic acid dropwise. This process requires strict control of the reaction temperature to prevent side reactions.
After the dropwise addition is completed, continue to stir for a period of time to promote the full reaction of the two. After the reaction, the reaction solution is cooled and then extracted with a suitable organic solvent to remove impurities.
After extraction, the organic phase is collected and the organic solvent is removed by vacuum distillation to obtain crude MES.
To obtain high-purity MES, further refining is required. The crude product can be dissolved in an appropriate amount of water and decolorized by activated carbon to remove colored impurities. Then, the recrystallization operation is carried out. Select a suitable solvent, control the crystallization temperature and speed, and obtain pure MES crystals.
Finally, the crystals are filtered and dried to obtain the finished MES. During the preparation process, factors such as temperature, material ratio and reaction time have a great impact on the purity and yield of the product, and need to be carefully controlled.
What are the differences in the application of Mes 2- (N-Morpholino) Ethanesulfonic Acid in different fields
MES, which is 2- (N-morpholino) ethanesulfonic acid, is widely used in the fields of biochemistry and molecular biology, with different applications.
In the field of cell culture, MES is often used as a buffer. Cells are extremely sensitive to the pH value of the growth environment, and MES can maintain a stable pH range, generally between 6.0 and 7.0, which is suitable for most cell growth. Because of its good buffering capacity, it can resist pH fluctuations caused by cell metabolism to produce acid or alkali, create a stable microenvironment for cells, and ensure normal cell growth and metabolism. For example, in culturing some adherent cells that are sensitive to pH changes, the addition of MES buffer system can significantly improve cell survival and proliferation.
In the enzyme reaction system, MES plays a key role. Many enzymatic reactions have strict pH requirements, and MES buffers can precisely regulate pH on demand to ensure that enzymes are in the best active state. Different enzymes have specific optimal pH, such as some acid phosphatases with the strongest activity when the MES buffer pH is about 5.5, providing suitable conditions for related research and applications to ensure efficient and stable enzymatic reactions.
In nucleic acid electrophoresis experiments, MES is also indispensable. Nucleic acid electrophoresis requires a specific pH environment to ensure stable migration and separation of nucleic acid molecules. The MES buffer system can provide suitable pH and ionic strength, so that nucleic acid molecules can migrate orderly in the gel according to their own size and charge characteristics, so as to achieve accurate separation and analysis. For example, when separating small RNA fragments, the electrophoresis buffer containing MES can achieve ideal resolution and band clarity.