Mops, Sodium Salt 3- (N-Morpholino) Propanesulfonic Acid, What is the main use of Sodium Salt?
Mops, which is the sodium salt of 3- (N-morpholino) propane sulfonate, has a wide range of main uses.
In the field of biochemistry and molecular biology experiments, it is often used as a buffer. Because it can effectively maintain the specific pH value of the solution, it provides a suitable acid-base environment for the stable existence and normal function of biological macromolecules. Many enzymatic reactions, protein and nucleic acid related experiments require precise control of pH, and Mops is indispensable for this. For example, in the reaction system of some DNA-restricted endonuclease enzymes, the use of Mops buffer can ensure the optimal activity of the enzyme, so that the enzyme can achieve precise cleavage of specific DNA sequences.
In cell culture, Mops also plays a key role. When cells are cultured in vitro, they are extremely sensitive to environmental pH. Mops can help the culture medium maintain a stable pH, which fits the microenvironment required for cell growth. For example, when culturing mammalian cells, the Mops buffer system can allow cells to proliferate and metabolize normally under relatively stable acid-base conditions, reducing the abnormal growth or death of cells due to pH fluctuations.
In addition, in electrophoresis experiments, Mops buffer is used to prepare gels and electrophoresis buffer systems. It can ensure the stable migration of biological macromolecules such as proteins and nucleic acids in the electric field, and achieve effective separation according to their own charge and size differences. Taking SDS-polyacrylamide gel electrophoresis as an example, suitable Mops buffer can make protein subunits appear clearly on the gel according to molecular weight, which helps researchers to accurately analyze protein composition and characteristics.
Mops, Sodium Salt 3- (N-Morpholino) Propanesulfonic Acid, What are the chemical properties of Sodium Salt?
3 - (N - Morpholino) Propanesulfonic Acid (Mops, Sodium Salt 3- (N - Morpholino) Propanesulfonic Acid, Sodium Salt), which is a commonly used buffer in biochemical research. It has many chemical properties, so let me tell you one by one.
This compound is often white crystalline powder in appearance, stable in properties, easy to store and use. It has good solubility in water, which makes it easy to prepare buffer solutions of the required concentration.
From the perspective of chemical structure, its molecules contain morpholine ring and propanesulfonic acid sodium structure. The morpholine ring gives it certain stability and specific spatial structure, while the sodium propanesulfonic acid structure determines its water solubility and buffering properties.
In terms of buffering properties, this compound exhibits good buffering ability in the pH range of 6.5-7.9, which can effectively maintain the stability of solution pH. Under the changeable biochemical experimental environment, it can ensure that the pH value of the reaction system is in an appropriate range, and ensure that the structure and function of biological macromolecules (such as proteins, nucleic acids, etc.) are not affected by large fluctuations in pH.
In addition, the chemical properties of 3- (N-morpholino) propionate sodium salt are relatively mild, and it has good compatibility with most biomolecules. It is not easy to chemically react with proteins, nucleic acids, etc., so it does not interfere with the normal biochemical reaction process in organisms. Therefore, it is often used in cell culture, enzyme reaction, electrophoresis and other biochemical experimental systems.
Mops, Sodium Salt 3- (N-Morpholino) Propanesulfonic Acid, Sodium Salt What are the precautions when storing?
Mops, that is, 3 - (N - morpholino) propane sulfonate sodium salt, when storing, pay attention to many things.
First, check its temperature and humidity. This compound should be stored in a cool and dry place, because the temperature is too high or it deteriorates, and the humidity is too high or it absorbs moisture, which affects the quality. For example, "Tiangong Kaiwu" talks about material preservation, the temperature and humidity of the environment are the key, which is related to the characteristics and life of the material.
Second, it should be avoided from contact with oxidants, acids and other substances. The chemical properties of Mops determine that if they coexist with such substances, it is easy to cause chemical reactions, damage their chemical structure, and cause their failure. In the past, when preserving medicines, they also knew to place them separately to prevent their interaction.
Third, storage containers are also exquisite. Use a well-sealed container to block the intrusion of air, water vapor, etc. If the container is not well sealed, external substances are easy to interact with Mops, destroying its stability. Ancient treasures also pay attention to the sealing of utensils, so that the preserved objects can last for a long time.
Fourth, pay attention to the shelf life. Although Mops have a certain stability, it may take too long, or it may change qualitatively due to its own slow reaction. Therefore, it is necessary to check in a timely manner according to its production batch and instructions to avoid failure. The ancients also had a method of regular inspection of items to ensure their usability.
Mops, Sodium Salt 3- (N-Morpholino) Propanesulfonic Acid, What happens when Sodium Salt is mixed with other substances?
Mops sodium salt, that is, 3 - (N - morpholino) propanesulfonate sodium salt, is chemically stable. When mixed with other substances, the reaction that occurs depends on the chemical properties of the mixed substances.
If mixed with acids, because Mops sodium salt is weakly basic, acid-base neutralization will occur. For example, in the case of hydrochloric acid, the two will react to generate corresponding salts, water, and released heat. The reaction formula is roughly: 3 - (N - morpholino) propanesulfonate sodium salt + HCl → 3 - (N - morpholino) propanesulfonate + NaCl.
When mixed with a metal salt, if the metal ion in the metal salt can react with certain groups in the sodium salt of 3- (N-morpholino) propionate or form a precipitate, it will produce corresponding changes. For example, with some transition metal salts, such as copper salts, it is possible to form coordination compounds that change the color and chemical properties of the solution.
If mixed with a strong oxidizing agent, some groups in the sodium salt of Mops may be oxidized, causing its structure and properties to change. When mixed with a strong reducing agent, under suitable conditions, some groups may be reduced.
In addition, if mixed with a specific enzyme or biologically active substance, in an organism or in a simulated biological environment, it may affect the relevant biochemical reaction process, or change the activity of the enzyme due to binding with the activity check point of the enzyme. In short, the reaction of Mops sodium salt mixed with other substances is rich and diverse, and the specific reaction needs to be comprehensively determined according to the type of mixed substance, reaction conditions and other factors.
What is the market price of Mops, Sodium Salt 3- (N-Morpholino) Propanesulfonic Acid, Sodium Salt?
The market price of Mops, the sodium salt of 3 - (N - morpholino) propanesulfonic acid, varies due to various factors and is difficult to determine.
If you explore its price in the context of "Tiangong Kaiwu", although there was no such fine chemical at that time, you can compare it according to the rarity and simplicity of the product. At present, this product is mostly chemical synthesis and is widely used in experiments, medicine and other fields.
Its price is related to purity, packaging specifications, purchase volume and suppliers. Those with high purity, such as for high-end scientific research experiments, are expensive; common analytical purity grades, each bottle (such as 250 grams) or between tens of yuan and hundreds of yuan. If you wholesale in large quantities, the unit price may be reduced. When chemical reagent companies sell, small packages are expensive to retail, and large packages are cheap to wholesale. Imported products may be more expensive than domestic products due to quality control and transportation costs.
For example, if a domestic ordinary purity, 250 grams, the price may be 60-80 yuan; imported high-purity packaging of the same amount, or 150-200 yuan. And if you buy several kilograms, the wholesale price may drop to 200-400 yuan per kilogram. Therefore, if you want to know the exact price, you need to consult the relevant suppliers and ask in detail according to your own needs.