N-(2-Acetamido)-2-Aminoethanesulphonic Acid

N- (2 -acetamido) -2 -aminoethanesulfonic acid, its chemical structure is as follows:
The main chain of this compound is ethanesulfonic acid structure, and the sulfonic acid group (-SO < H >) is connected to one end of ethane. On the carbon atom at the other end of ethane, there is an amino group (-NH2O) connected, and the nitrogen atom in the amino group is connected to the acetyl group (CH < CO >) to form an acetamido group (-NHCOCH < O >). The overall chemical structure can be written as: CH
This structure gives the compound specific chemical properties. The sulfonic acid group makes it acidic to a certain extent and has good water solubility. The existence of acetamide and amino groups affects its acidity and alkalinity and reactivity. This structural characteristic is of great significance in the fields of biochemistry and medicine, and is often used as a biological buffer because its structure can effectively maintain the stability of the pH of the system within a specific pH range, providing a suitable acid-base environment for biochemical reactions.

N- (2-acetamido) -2-aminoethanesulfonic acid, or ACES, is widely used in biological experiments.
One of them is often a buffer. In many biochemical reactions and analytical tests, a constant pH environment is required to ensure the stability of biomolecular structure and function. The buffer range of ACES is about pH6.1-7.5, which meets the physiological pH requirements of many biological systems. For protein and enzyme related experiments, proteins can maintain their natural conformation and activity within this pH range. If the pH deviates from the appropriate range, proteins may denature and enzyme activity may be inhibited. Like enzymatic reaction experiments, ACES can create a stable pH environment, allowing enzymes to efficiently catalyze reactions, and help researchers accurately determine enzyme kinetic parameters.
Second, for cell culture. When cells are cultured in vitro, it is necessary to accurately simulate the physiological environment in vivo, and pH stability is essential. ACES can adjust the pH of the culture medium to provide a good environment for cell growth and proliferation. Different cell lines have different pH requirements. ACES buffering capacity can meet the specific pH requirements of some cells, ensure normal metabolism and function of cells, and prevent cell damage or death due to pH fluctuations.
Third, it is also used in the field of nucleic acid research. In experiments such as nucleic acid extraction, amplification and electrophoresis, suitable pH is of great significance to maintain nucleic acid stability and structural integrity. The ACES buffer system can ensure pH stability of the reaction system and avoid nucleic acid degradation or structural changes. For example, in PCR reactions, accurate pH environment is the key to the activity of Taq enzyme. ACES can help optimize reaction conditions and improve amplification efficiency and specificity.

N- (2-acetamido) -2-aminoethanesulfonic acid, this is a very important organic compound. Its solubility is closely related to many factors.
In water, the substance exhibits a certain solubility. Because its molecular structure contains polar groups, such as amino and sulfonic acid groups, both of which can be combined with water molecules by hydrogen bonds, they can promote their dissolution in water. Under suitable temperature conditions, an appropriate amount of N- (2-acetamido) -2-aminoethanesulfonic acid can be well soluble in water to form a uniform solution.
However, if the temperature is too low, the thermal motion of the molecule weakens, its solubility may decrease, and some solutes may precipitate from the solution. On the contrary, appropriately increasing the temperature can usually enhance its solubility. Due to the increase in temperature, the thermal motion of the molecule can be intensified, which is conducive to the interaction between the solute molecule and the water molecule, and then more dissolution.
As for organic solvents, their solubility varies depending on the type of solvent. Polar organic solvents such as ethanol also have certain interactions with the molecules of the compound, so N - (2-acetamido) -2-aminoethanesulfonic acid also has a certain solubility in them. However, in non-polar organic solvents, such as n-hexane, due to the difference in molecular forces, the solubility of the compound is extremely poor and almost insoluble.
In summary, the solubility of N- (2-acetamido) -2-aminoethanesulfonic acid is significantly affected by factors such as solvent type and temperature, and exhibits good solubility in polar solvents and suitable temperatures.

N- (2-Acetamido) -2-aminoethanesulfonic acid, often referred to as ACES, is a commonly used zwitterion buffer in biochemical research. Its storage conditions are quite critical, as detailed below:
bear the brunt, pay attention to the control of temperature. It should be stored in a cool place, the best temperature range is about 2-8 ° C. This temperature range can effectively slow down the chemical changes that may occur, inhibit the decomposition or deterioration caused by excessive temperature. If the temperature is too high, the activity or structure of ACES molecules may be damaged, resulting in a decrease in buffering performance. For example, if placed in a high temperature environment, the chemical bonds in ACES molecules may be broken due to intensified thermal motion, affecting their normal function.
Secondly, the humidity should not be ignored. A dry storage environment should be kept to avoid moisture. Because of its certain water absorption, the purity may be reduced after moisture, which affects the accuracy of the experimental results. Assuming that the storage environment humidity is high, ACES may absorb moisture and change its own concentration, and the moisture may participate in its chemical reaction to produce impurities.
Furthermore, attention should be paid to isolating the air. ACES may react with oxygen, carbon dioxide and other components in the air. For this purpose, it can be placed in a sealed container. For example, use a sealed glass bottle or plastic bottle to reduce contact with air and prevent oxidation and other reactions to maintain its chemical stability.
In addition, the storage place should avoid direct light exposure. Light or luminescence chemical reactions can adversely affect the structure and properties of ACES. Therefore, it is better to store in an opaque container or in a dark place.
Strictly follow the above storage conditions to ensure that N- (2-acetamido) -2-aminoethanesulfonic acid maintains good quality and performance for a long time to meet the needs of various biochemical experiments.

N- (2-acetamido) -2-aminoethanesulfonic acid, or ACES, its related organisms mostly exist in biochemical experiments and cell culture environments.
Guanfu ACES, in biological systems, is often used as a buffer. In the context of cell culture, it can stabilize its acid-base degree, protect cells in a suitable acid-base atmosphere, and if the cell is housed, it will be protected from large changes in pH.
In enzymatic experiments, the activity of many enzymes depends on the appropriate pH to achieve its full function. ACES can create suitable acid-base conditions, so that enzymes can develop their catalytic ability, just like a good craftsman's adapter, which is easy to achieve. Such as some proteases, in the annulus stabilized by ACES, the hydrolysis of proteins is quite effective.
And in protein electrophoresis experiments, ACES is also indispensable. It can maintain the acid-base balance of the electrophoresis system, so that the protein swims orderly in the gel medium according to its inherent charge and size, so as to form a clear band, so that the researcher can analyze it. If it is divided into threads by longitude and latitude, the structure will appear.
Furthermore, in the nucleic acid extraction and analysis experiments of molecular biology, the buffer environment made by ACES can keep the structure of the nucleic acid stable and prevent its degradation, and keep the pearl in the box, so that it is intact for subsequent research, such as PCR amplification, sequencing, etc., all rely on this stable environment.