N- (2-Aminoethyl) -3-Aminoethanesulphonic acid, Sodium salt What are the common application fields
N- (2-aminoethyl) -3-aminoethanesulfonic acid, sodium salt, often used in biochemical and medical experimental fields.
In protein and enzyme research, this is a commonly used buffer. Proteins and enzymes are extremely sensitive to the pH of their environment, and if there is a slight deviation, their structure and function will be affected. This sodium salt can maintain the stability of the pH of the system in a specific pH range, just like building a stable "small environment" for proteins and enzymes, ensuring that they can display their activity and function normally during the experimental process, so that researchers can accurately explore their characteristics and mechanisms of action.
The field of cell culture is also indispensable. Cell growth requires a suitable acid-base environment. As a buffer substance, this sodium salt can resist pH changes caused by external factors, ensure cell growth and proliferation in a stable environment, and help researchers obtain reliable cell experimental results. Experiments such as cell metabolism research and drug screening depend on this.
In addition, it also plays a key role in the test methods of clinical diagnosis. Many tests require precise control of the pH of the reaction system. This sodium salt can ensure the stability of pH during the test process, improve the accuracy and reliability of the test, and is of great significance for disease diagnosis and monitoring.
In summary, N- (2-aminoethyl) -3-aminoethanesulfonic acid, sodium salt, with its excellent buffering properties, plays an important role in many key links of biochemical and medical experiments, providing strong support for the smooth advancement of scientific research and clinical work.
What are the chemical properties of N- (2-Aminoethyl) -3-Aminoethanesulphonic acid, Sodium salt
N- (2-aminoethyl) -3-aminoethyl sulfonic acid, sodium salt, this is a very important compound. Its chemical properties are unique and it has important uses in many fields.
Looking at its physical properties, it is a white crystalline powder under normal conditions, with good solubility and easy solubility in water. This property makes it easy to disperse and participate in reactions in aqueous solution systems or applications.
In terms of chemical properties, due to the presence of amino groups and sulfonic acid groups in the molecule, it presents a unique chemical activity. Amino groups are alkaline and can neutralize with acids to form corresponding salts. This property allows it to interact with acidic substances under specific conditions to regulate the pH of the system. The sulfonic acid group makes the compound hydrophilic and ionic, which helps it to dissociate in aqueous solution and participate in reactions such as ion exchange.
In addition, the stability of the compound is also worthy of attention. Under normal environmental conditions, its chemical properties are relatively stable. However, under extreme conditions such as specific strong oxidants, strong acids or strong bases, chemical reactions may occur, resulting in changes in molecular structure.
Because of its unique chemical properties, it can be used in the field of biochemistry or as a buffer to maintain the pH stability of the reaction system and ensure the activity and function of biological macromolecules. In the industrial field, or in the preparation of certain special materials, it plays a key role in helping to optimize the properties of materials.
N- (2-Aminoethyl) -3-Aminoethanesulphonic acid, Sodium salt What are the precautions during storage
N- (2-aminoethyl) -3-aminoethyl sulfonate sodium during storage, many matters need to be paid attention to. This agent is inherently stable, but if it is not stored properly, it will also change.
The first storage environment must choose a cool, dry and well-ventilated place. Avoid high temperature and humidity to prevent moisture decomposition or deterioration. If it is in a high temperature environment, its chemical structure may be damaged, and the efficacy will be greatly reduced; in a humid place, it is easy to absorb moisture and agglomerate, which affects the quality.
Furthermore, it should be separated from oxidizing, reducing substances and strong acids and bases. The chemical activity of this agent makes it easy to react with various substances. If it is mixed, it may react violently, endangering safety or damaging its chemical properties.
The sealing of the package is also critical. After taking it, seal the package immediately to prevent air and water vapor from invading. The package is damaged, easy to contact with external substances, and accelerates deterioration.
and when storing, it should be checked regularly. Look at its appearance, such as color, morphology, if the color changes, agglomeration, etc., or has deteriorated.
In addition, detailed records of storage conditions, such as temperature, humidity, and storage start date. This is convenient for traceability and control, and is also an important way to ensure its quality and stability.
Store sodium N - (2 -aminoethyl) - 3 -aminoethyl sulfonate, and observe the above things to ensure its stability and quality during storage.
What is the preparation method of N- (2-Aminoethyl) -3-Aminoethanesulphonic acid, Sodium salt
N- (2-aminoethyl) -3-aminoethyl sulfonic acid, sodium salt, the preparation method is as follows:
First take an appropriate amount of 2-aminoethyl mercaptan and place it in a clean reaction vessel. This is the starting material for the reaction. Careful measurement is required to ensure that the dosage is accurate.
Add a certain amount of acrylonitrile slowly dropwise to the above container containing 2-aminoethyl mercaptan. When adding dropwise, control the rate and do not make it too fast to prevent the reaction from being too violent. This step of the reaction aims to build a molecular skeleton, and the two react slowly to generate a specific intermediate.
After the acrylonitrile is added dropwise, maintain the reaction system at a suitable temperature, and continue to stir for a period of time to allow the reaction to proceed sufficiently, so that the reactants can be converted into the target intermediate as much as possible.
Then, transfer the resulting intermediate to another reaction device, add an appropriate amount of sodium hydroxide solution to it, and carry out the hydrolysis reaction. In this process, the temperature and reaction time need to be strictly controlled. Too high or too low temperature, too long or too short time may affect the purity and yield of the final product.
After the hydrolysis reaction is completed, the reaction mixture should be treated. First, the insoluble impurities in it are removed by suitable methods, such as filtration, to clarify the solution.
Then, using ion exchange resin and other technologies, the solution is purified to remove excess ions to improve the purity of the product.
Finally, through evaporation, concentration, cooling and crystallization, N- (2-aminoethyl) -3-aminoethanesulfonic acid and sodium salt are crystallized from the solution. After suction filtration, washing, drying and other operations, pure N- (2-aminoethyl) -3-aminoethanesulfonic acid and sodium salt products are obtained. Throughout the preparation process, each step requires fine operation and attention to the control of reaction conditions to obtain high-quality products.
What are the reactions of N- (2-Aminoethyl) -3-Aminoethanesulphonic acid, Sodium salt and other compounds?
Sodium N- (2-Aminoethyl) -3-aminoethanesulfonate (N- (2-Aminoethyl) -3-Aminoethanesulphonic acid, Sodium salt) is a useful compound that can play a unique role in many chemical reactions. When
reacts with acids, its molecules contain both amino groups and sodium sulfonate groups, and the amino groups are basic, which can neutralize with acids to form corresponding salts. For example, when reacting with hydrochloric acid, the amino group can combine hydrogen ions to form positively charged ammonium ions, which are paired with chloride ions to form salts. This reaction is often used to adjust the pH of the system or to prepare specific organic salt products.
In the case of alkali, although the sulfonic acid part of the compound is relatively stable, under certain conditions and the action of strong bases, the sodium salt of the sulfonic acid group may undergo partial transformation. For example, at high temperatures and high concentrations of strong bases, the sulfonic acid group may undergo a little hydrolysis or other complex reactions, but such reactions usually require more harsh conditions.
In the field of organic synthesis, its amino group can participate in the reaction as a nucleophilic reagent. If it encounters a halogenated hydrocarbon, the nitrogen atom in the amino group will attack the carbon atom of the halogenated hydrocarbon with its lone pair electron, and the halogen atom will leave, and a nucleophilic substitution reaction will occur, thereby forming a new carbon-nitrogen bond. This is an important strategy for synthesizing nitrogen-containing organic compounds.
The compound can also coordinate with metal ions. Because both amino and sulfonic acid groups have the ability to give electrons, they can form stable complexes with some metal ions (such as copper ions, zinc ions, etc.). This property is widely used in catalytic reactions or material preparation, and metal complexes may exhibit catalytic activities or physical properties that are completely different from the original compounds.
In addition, in biochemical research, sodium N- (2-aminoethyl) -3-aminoethanesulfonate is often used as a buffer component due to its good water solubility and relatively mild chemical properties. In the research experiments of biological macromolecules such as proteins and enzymes, the pH value of the system is adjusted to maintain the stability of the reaction environment and ensure the activity and structural integrity of biomolecules.