What is the chemical structure of N-2-Hydroxy-3- [[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Amino] Propanesulphonic Acid
This is N-2-hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propanesulfonic acid, which has a complex and delicate molecular structure. In this compound, the main body is propanesulfonic acid structure, and the sulfonic acid group (-SO-H) is attached to propane, giving it acidic properties and specific chemical activity. The No. 3 position of propane is connected with a nitrogen-containing group, which is composed of 2-hydroxy-1,1-bis (hydroxymethyl) ethyl groups connected by amino groups. In the 2-hydroxy-1,1-bis (hydroxymethyl) ethyl group, the 1 position of the ethyl group is connected with two hydroxymethyl groups (-CH 2O OH), and the 2 position has a hydroxy group (-OH), which enhances the hydrophilicity and reactivity of the molecule. The amino group (-NH) connected to propane is a key bridge connecting the two parts of the structure, which has an important impact on the overall chemical properties, spatial structure and reaction characteristics of the molecule. This complex structure makes the compound exhibit unique properties in many chemical and biological fields, or can be used for specific chemical reactions or play specific roles in biological systems.
What are the main uses of N-2-Hydroxy-3- [[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Amino] Propanesulphonic Acid
N-2-hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, a biological buffer, is widely used in the field of biochemical research.
It has many main uses. In biochemical experiments, it can accurately maintain the pH value of the solution. Many biochemical reactions are extremely sensitive to pH value, and even a slight change may have a significant impact on the reaction process and results. And this substance can effectively resist pH fluctuations caused by external acid-base or chemical reactions within a specific pH range by virtue of its own characteristics, ensuring that the reaction can be carried out smoothly in a suitable pH environment.
In protein research, the structure and function of proteins are closely related to the pH value of the environment in which they are located. The use of this substance to create a stable pH condition helps to maintain the natural conformation and biological activity of proteins and prevent them from denaturing due to pH discomfort, thus enabling researchers to more accurately explore the properties and functions of proteins.
Cell culture is also one of its important application scenarios. When cells are cultured in vitro, the environmental pH requirements are strict. It can create pH conditions similar to the living environment in the cell body, provide a suitable acid-base environment for physiological activities such as cell growth and metabolism, and ensure the normal proliferation and function of cells. It is of great significance for the study of cell biology.
In addition, in the study of enzymatic reactions, the activity of enzymes is usually restricted by pH value. By using this buffer to stabilize the pH value of the reaction system, the enzyme can be in the best active state, which is convenient for researchers to study the kinetic parameters of enzymes, substrate specificity and other characteristics, and further clarify the mechanism of action of enzymes in vivo.
What are the differences in the application of N-2-Hydroxy-3- [[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Amino] Propanesulphonic Acid in different fields?
N - 2 - hydroxy - 3 - [ [ 2 - hydroxy - 1,1 - bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, often referred to as HEPPSO. This substance is used in different fields and its application varies greatly.
In the field of biochemistry and molecular biology, it is often used as a buffer. Because of its good buffering performance, it can maintain the pH stability of the reaction system, just like an anchor for a stable boat. Many biochemical experiments, such as protein and nucleic acid studies, are extremely sensitive to pH. HEPPSO can precisely regulate the pH of the environment, preserve the structure and activity of biological macromolecules, and help the experiment proceed smoothly.
In the field of medical testing, it is also indispensable. Among diagnostic reagents, it can create a suitable pH environment and improve the accuracy and reliability of detection. Just like the determination of clinical enzyme activity, precise pH conditions are the key to accurate results. HEPPSO takes on the important task of regulating pH here, providing a solid support for disease diagnosis.
In the field of cell culture, HEPPSO also plays an important role. Cell growth requires strict environmental requirements, and the pH value needs to be stabilized within a specific range. It can maintain the pH stability of the culture medium, create a comfortable growth home for cells, promote cell proliferation and normal metabolism, and help cell culture research to advance steadily.
In industrial production, especially in the manufacture of some fine chemical products, HEPPSO is used as an auxiliary agent. It can improve product performance, such as adjusting the pH of the reaction system to affect product purity and quality, and play a unique role in improving product quality.
What is the production process of N-2-Hydroxy-3- [[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Amino] Propanesulphonic Acid
N - 2 - hydroxy - 3 - [ [ 2 - hydroxy - 1,1 - bis (hydroxymethyl) ethyl] amino] propane sulfonic acid, often abbreviated as TES, the preparation process is described later:
The first is 2 - amino - 2 - hydroxymethyl - 1,3 - propanediol as the starting material, which is the basis of the reaction. It is carefully placed in a suitable reactor with good temperature control and stirring ability. Slowly inject a certain amount of 3-chloro-2-hydroxypropanesulfonic acid into the kettle. The ratio of the two needs to be precisely pinched. According to the ancient method, the ratio is related to the purity and quantity of the product, so it needs to be carefully measured.
Next, add an appropriate amount of acid binding agent, such as potassium carbonate, which can trap the generated acid during the reaction and make the reaction go smoothly to the right. In this process, it is necessary to pay close attention to the change of temperature, stabilize the temperature of the system with a water bath or an oil bath, and control it within a specific temperature range according to the past practice, so that the reaction can be efficient and orderly. During the reaction, continue to stir the material to make full contact and accelerate the reaction.
After the reaction is completed, a thick reaction solution is retained by vacuum distillation to remove excess solvent and unreacted raw materials in the system. After that, the reaction solution is purified, and ion exchange resin chromatography is often used. According to the affinity difference between ions, the target product and impurities are separated. After this purification, pure N-2-hydroxy-3 - [ [ 2-hydroxy-1,1-bis (hydroxymethyl) ethyl] amino] propane sulfonic acid can be obtained. Finally, the residual moisture in the product is removed by low temperature drying to obtain a white powder-like finished product, which is the desired product.
What is the market outlook for N-2-Hydroxy-3- [[2-Hydroxy-1,1-Bis (Hydroxymethyl) Ethyl] Amino] Propanesulphonic Acid
N - 2 - hydroxy - 3 - [ [ 2 - hydroxy - 1,1 - bis (hydroxymethyl) ethyl] amino] propanesulfonic acid, this substance is widely used in the field of biochemical research and is often used as a biological buffer. Looking at its market prospects, it can be said to be bright.
Today, life science is developing rapidly, and the fields of biopharmaceuticals, gene testing, and cell culture are booming. In biopharmaceuticals, the production process of proteins and antibodies requires precise control of the pH of the reaction system. With its excellent buffering performance, this buffer can effectively maintain the stability of pH value of the system, ensure the activity and structural integrity of biological macromolecules, and help the production of high-quality drugs. Therefore, the demand in the biopharmaceutical industry is growing.
In the field of gene detection, PCR amplification, sequencing and other experimental steps have strict requirements on the reaction environment. The buffer can provide a suitable and stable acid-base environment to ensure the accuracy and reliability of the test results. With the popularization of gene detection technology in disease diagnosis and personalized medicine, the demand for it is also on the rise.
Furthermore, cell culture is a key link in basic research in life sciences and the production of biological products. Cell growth is extremely sensitive to environmental pH. This buffer can create a stable microenvironment, promote cell growth and proliferation. The development of cell therapy, vaccine research and development and other industries has also promoted its market demand to rise steadily.
In addition, with the increase in scientific research investment and the continuous innovation of technology, more new products and new applications based on this buffer are expected to be developed, and its market prospect will become increasingly broad, and it will definitely shine in the future life science field.