N- (2-Hydroxyethyl) Piperazine-N '-2-Ethanesulfonic Acid, What are the common uses of Sodium Salt
N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt, commonly used. In the field of biochemical research, it can be used as a chemical. The biochemical reaction of biological organisms needs to be carried out in a specific pH environment. This compound can effectively maintain the pH of the anti-chemical system, just like a guardian of the city, to maintain the order of biochemical reaction.
It is also indispensable in an important field of cell culture. Cell survival and proliferation, the environment is demanding, and some microwaves at pH value may affect the life of the cell. This, N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt can be effective in determining the pH environment, such as warming the nest, and preventing the growth of cells.
Furthermore, in protein research, the function of protein is susceptible to environmental factors. This compound can pass the pH value, avoid protein degradation due to pH degradation, and ensure the development of protein research. Just like protein research, it is a smooth road. In addition, it plays an important role in biochemical, cellular and protein research.
N- (2-Hydroxyethyl) Piperazine-N '-2-Ethanesulfonic Acid, Sodium Salt What are the storage conditions?
N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt, that is, HEPES sodium salt, its storage conditions are quite important. This is a commonly used buffer in biochemical experiments, and it needs to be carefully stored to ensure its quality and performance.
HEPES sodium salt is very easy to absorb moisture, so it should be stored in a dry place. If the air humidity is high, it is easy to absorb moisture from the air, causing its properties to change and affecting the experimental effect. It should be placed in a dryer with a desiccant, such as color-changing silica gel, to monitor and maintain the drying environment. Once the silica gel changes color, it needs to be replaced in time to maintain drying efficiency.
Temperature is also a key factor. Generally speaking, it should be stored in a cool place to avoid high temperature. High temperature may cause its decomposition, or accelerate its chemical change, which will damage its buffering performance. Usually refrigerated at 2-8 ° C, if long-term storage is required, freezing at -20 ° C is better. However, after freezing, it is necessary to pay attention to slow thawing to prevent sudden temperature changes from affecting its quality.
In addition, HEPES sodium salts should avoid contact with strong oxidants, strong acids, strong bases and other substances. Due to their chemical properties or reactions with these substances, they will fail. The storage container should also be corrosion-resistant and well-sealed, such as glass bottles or high-quality plastic bottles, to prevent chemical reactions between the container and the drug, and a good seal can block air and moisture. Following the above storage conditions can make the HEPES sodium salt play its due role in the experiment and ensure the accuracy and reliability of the experimental results.
What are the chemical properties of N- (2-Hydroxyethyl) Piperazine-N '-2-Ethanesulfonic Acid, Sodium Salt?
N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonate sodium salt, its chemical properties can be investigated. This salt has good buffering properties and is commonly used in biochemical research. Its aqueous solution is mildly buffered, which can maintain the stability of a specific pH range, just like the ancient stable rudder boat, to ensure the smooth biochemical reaction.
Looking at its physical properties, it is usually a white crystalline powder, easily soluble in water, like snow melting in a river, quickly dispersed in water to form a uniform liquid phase. And this salt has good chemical stability. It is not easy to react violently with surrounding substances at normal temperature, just like a hermit sitting in a corner, guarding its own nature.
In addition, it has a certain hydrophilicity. Because the molecular structure contains hydrophilic groups such as hydroxyl groups and sulfonic acid groups, it is like a good friend of water relatives, so it can be well dispersed in the water system and play its buffering effect in many aqueous environments of biochemical experiments.
In an acid-base environment, this salt can release or accept protons appropriately according to environmental changes to maintain the pH of the system and ensure the orderly operation of biochemical reactions. It is indispensable in the biochemical field.
How does N- (2-Hydroxyethyl) Piperazine-N '-2-Ethanesulfonic Acid, Sodium Salt react with other reagents?
N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid, sodium salt, often referred to as HEPES sodium salt, is a commonly used buffer in biochemical research. Its reaction with other substances is quite complicated and varies depending on the reaction object.
In case of strong acids, such as hydrochloric acid, this sodium salt will exhibit alkaline properties and undergo acid-base neutralization with it. The neutralization ability of HEPES sodium salt is derived from the basic groups contained in its molecular structure, which can receive protons and form corresponding conjugated acids. After the reaction, the conjugated acid of HEPES and the chloride of sodium salt will be produced.
If it meets a strong base, such as sodium hydroxide, because there are ionizable groups such as hydroxyl groups and sulfonic acid groups in the molecule, HEPES sodium salts may exhibit acidity and react with bases to generate corresponding salts and water.
In addition, in biochemical systems, HEPES sodium salts may also interact with biological macromolecules such as proteins and nucleic acids. Due to the presence of many charged and polar groups on the surface of biological macromolecules, the ionic and polar parts of HEPES sodium salts may interact with biological macromolecules through electrostatic interaction, hydrogen bonds, etc., which affect the structure and function of biological macromolecules.
At the same time, in some chemical reactions, HEPES sodium salt may act as a catalyst, relying on its specific chemical structure to promote or regulate the reaction process. However, such catalytic effects are usually more specific and only effective for some specific types of reactions. In short, the reaction of HEPES sodium salt with other reagents varies according to the specific situation. In practical application, the reaction conditions and the characteristics of the object need to be carefully considered.
How to choose the concentration of N- (2-Hydroxyethyl) Piperazine-N '-2-Ethanesulfonic Acid and Sodium Salt in different experiments
The choice of concentration of N- (2-hydroxyethyl) piperazine-N '-2-ethanesulfonic acid (HEPES) sodium salt in different experiments depends on the experimental purpose, sample characteristics, reaction system and many other factors.
If it is a cell culture experiment, because it needs to create a stable extracellular acid-base environment, the concentration is generally 10-50 mM. This concentration range can effectively maintain the pH value of the culture medium between 7.2 and 7.4, which meets the physiological needs of most cells and ensures the normal growth, metabolism and function of cells.
In the enzymatic reaction experiment, the concentration of HEPES sodium salt depends on the enzyme characteristics and reaction conditions. Usually 20-100 mM. For some pH-sensitive enzymes, the appropriate concentration of HEPES sodium salt can stabilize the pH value of the reaction system, maintain high activity and stability of the enzyme, and facilitate the efficient binding of substrates to enzymes and catalytic reactions.
Protein research experiments, such as protein purification, crystallization, etc., the concentration is usually 20-50 mM. The appropriate concentration not only maintains the pH stability of the protein solution and prevents protein denaturation, but also provides the appropriate ionic strength, which helps the protein maintain its natural conformation and function, and improves the purification efficiency and crystallization success rate.
Immunoassay experiments, such as enzyme-linked immunosorbent assay (ELISA), the concentration of HEPES sodium salt is generally 10-40 mM. This concentration range can stabilize the pH value of the reaction buffer, reduce non-specific binding, and improve the sensitivity and accuracy of the analysis.
In short, to choose the concentration of HEPES sodium salt, researchers need to comprehensively consider the specific requirements and conditions of the experiment, and determine the best concentration through pre-experimental optimization to achieve the best experimental effect.
