N-(2-Hydroxyethyl)Piperazine-N'-(2-Ethanesulfonic Acid) Sodium Salt

N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt has unique chemical properties. This substance is a white crystalline powder with high water solubility. When dissolved in water, it can quickly and uniformly disperse to form a clear solution.
From the perspective of acid-base characteristics, it is an excellent biological buffer. In a wide pH range, it can effectively maintain the pH stability of the solution. Near the physiological pH value, the buffering capacity is particularly strong, providing a stable acid-base environment for biochemical reactions. Many biological experiments and in vitro diagnostic reagents rely on this property.
In terms of thermal stability, under conventional experimental temperature and storage conditions, the properties are stable, and it is not easy to decompose when heated. Even if it is placed in a slightly higher temperature environment for a short time, the chemical structure and buffering properties are basically not affected, ensuring reliable performance during experiments and production.
Its chemical structure contains specific functional groups such as hydroxyl groups, piperazine rings and sulfonic acid groups. Hydroxyl groups enhance the hydrophilicity and hydrogen bond formation ability of molecules, which is of great significance for interactions with other biomolecules. Piperazine rings endow molecules with certain rigidity and spatial structure, which affects their conformation in solution and binding mode with other substances. Sulfonic acid groups are highly acidic and easily ionized in water, and play a key role in maintaining the ion balance and pH stability of buffer systems.
In addition, the substance is chemically mild, has no obvious chemical reaction or interference with most biomolecules, and has good compatibility with biological macromolecules such as proteins and nucleic acids, making it suitable for research and application in many biological-related fields.

N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt, often referred to as HEPES sodium salt. This agent has a wide range of uses and has made great contributions to the field of biochemistry and cell culture.
In the field of biochemical research, this agent can be used as an excellent buffer. Because it can effectively maintain the acid-base balance of the reaction system, it is like a boat in a wave, so that the biochemical reaction can proceed smoothly in a suitable pH. Many enzymatic reactions, protein and nucleic acid studies rely on it to create a stable environment, ensure the reaction is precise and orderly, just like a skilled craftsman carefully crafted, so that the biochemical phenomenon is true.
As for the field of cell culture, HEPES sodium salt is even more indispensable. Cell growth is extremely sensitive to the environment, and a slight deviation in pH is like a delicate flower meeting frost, or growth is hindered. This agent can make the pH of the culture solution constant, just like a loving father protecting his son, creating a stable home for cells. It can not only play a role in conventional culture conditions, but also maintain a stable pH even in special circumstances where gas exchange is limited, ensuring cell growth and helping researchers to travel smoothly in the process of cell research.

To prepare N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt, the following method can be used:
First take an appropriate amount of N- (2-hydroxyethyl) piperazine and 2-chloroethanesulfonic acid and place it in a clean reaction vessel. The ratio of the two should be precisely prepared according to the stoichiometric ratio to ensure that the reaction is fully carried out.
Then, add an appropriate amount of solvent, such as deionized water or a suitable organic solvent, to the container to evenly disperse the reactants and promote reaction contact.
After that, slowly add an appropriate amount of alkaline substances, such as sodium hydroxide. When adding alkali, it is necessary to pay attention to the change of the reaction temperature. The temperature can be controlled within a suitable range by means of a cooling device, usually 20-30 ° C. The addition of alkaline substances is designed to promote the reaction in the direction of generating sodium salts.
After adding alkali, turn on the stirring device to fully mix the reaction system. During the reaction process, closely observe the reaction phenomena, such as the color change of the solution and the formation of precipitation.
After the reaction continues for a period of time, the reaction process can be monitored by thin-layer chromatography or other suitable analytical methods. When the reaction reaches the expected level, that is, the raw materials are exhausted and the amount of product generated reaches the ideal value, the reaction is terminated.
Then, the reaction products are separated and purified. First, the solvent is removed by vacuum distillation to obtain the concentrated product crude. Then the crude product is purified by recrystallization, and a suitable solvent is selected, such as ethanol-water mixed solvent. After multiple recrystallization, pure N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt crystals can be obtained. Finally, the residual solvent in the crystal is removed by vacuum drying to obtain the finished product.

N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt, that is, HEPES sodium salt, there are many matters to pay attention to when storing.
This substance is quite sensitive to humidity and is very easy to absorb moisture. Therefore, it should be stored in a dry place, and it is necessary to ensure that the humidity of the storage environment is appropriate to prevent its properties from changing due to moisture absorption and affecting the subsequent use effect.
Temperature also needs to be carefully controlled. Generally speaking, it should be stored in a low temperature environment, generally 2-8 ° C. Excessive temperature may cause changes in its chemical properties and reduce its stability. If it is exposed to a high temperature environment for a long time, it may cause reactions such as decomposition, thus losing its original efficacy.
Furthermore, contact with oxidants and other substances should be avoided during storage. Due to the characteristics of its chemical structure, chemical reactions may occur in case of oxidants, resulting in deterioration. Therefore, it needs to be stored separately, away from oxidant chemicals to prevent interaction.
At the same time, the choice of storage container should not be ignored. It is advisable to choose a container with good sealing, which can effectively prevent moisture from invading and avoid unnecessary reactions with external substances. After taking it, the container should be sealed in time to ensure the stability of its storage environment. Only in this way can the quality and properties of N - (2-hydroxyethyl) piperazine - N '- (2-ethanesulfonic acid) sodium salt be maintained to the maximum extent for subsequent experiments or production needs.

N- (2-hydroxyethyl) piperazine-N '- (2-ethanesulfonic acid) sodium salt, commonly known as HEPES sodium salt, is an important reagent commonly used in biochemical research. In cell culture and other experiments, it plays a key role in maintaining a stable pH environment. Its price range is difficult to sum up, because many factors can be influenced.
First, depending on the manufacturer, different manufacturers, due to brand reputation, production process and cost control, pricing is quite different. Well-known large manufacturers, with their exquisite craftsmanship, strict quality control, product prices may be high; while emerging manufacturers, in order to compete for market share, may attract customers at lower prices.
Second, it depends on the packaging specifications. Usually, for small packages, the price per gram is higher due to the high unit production cost; for large packages, the unit cost is diluted and the price per gram is relatively low. For example, there must be a gap between the unit price of 5 grams and 500 grams.
Third, the market supply and demand situation is also a major factor affecting the price. If the market demand for the reagent is strong and the supply is limited, the price may rise; conversely, when the supply exceeds the demand, the price will fall.
In terms of common market conditions, for small packages (such as 5-25 grams), the price per gram may be in the tens of yuan; for medium packages (100-500 grams), the price per gram may be reduced to ten to ten yuan; for large packages (1 kg and above), the price per gram may be as low as ten yuan. But this is only a general range. When actually purchasing, inquiring about different suppliers can get accurate quotations.