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N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Tissue Culture What is the Chinese name of Tested?
This is N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid), which has been tested by tissue culture. It is quite important in the field of biochemistry and cell culture. This substance is often used as a buffer, which can stabilize and control the pH value in the cell culture ring, create a suitable acid-base atmosphere for cells, and enable cells to grow and metabolize in a stable environment. It has excellent buffering properties, which can cope with pH fluctuations caused by metabolic activities during cell culture and ensure the normal operation of cell physiological functions. And after tissue culture testing, it is proved that its purity and quality are of high standards. It can be used in cell culture experiments to reduce the interference and adverse effects of impurities on cells and improve the reliability and repeatability of experiments. It is an indispensable key reagent for cell culture and has been widely used in many fields such as cell biology research and biopharmaceuticals, assisting researchers in exploring the mysteries of cells and promoting the development of biomedicine.
N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Tissue Culture Tested for which tissue culture experiments
N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid), which has been tested by tissue culture, is often used in a variety of tissue culture experiments. This reagent is quite commonly used in cell culture experiments because it can effectively maintain the stability of the pH of the medium, just like an ancient pressure regulator, making the environment constant and suitable for cell growth.
In animal cell culture, such as common mammalian cells such as HEK293 cells and CHO cells, it can ensure that the pH of the medium is in a suitable range for cells, and help cells grow and proliferate well, just like fertile soil nourishes seedlings. In plant tissue culture, it can also play a role in stabilizing the acid-base environment for some sensitive plant cells or tissues, so that plant tissues can develop smoothly under in vitro culture conditions.
In the field of microbial cell culture, such as the culture of some special bacteria or fungi, this reagent also has its place, creating a stable living environment for microorganisms and assisting their reproduction and metabolism. In various tissue culture experiments, it is like a fixed needle in the sea, ensuring the stability of the experimental environment, promoting the smooth progress of the culture process, allowing researchers to better observe and study the growth characteristics and physiological functions of cells and tissues.
N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Tissue Culture Tested What is the purity requirement?
In the ancient method of "Tiangong Kaiwu", there are specific regulations on the purity requirements of N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid) (Tissue Culture Tested). This agent is used in tissue culture, which is related to the cultivation and reproduction of living cells, and the purity must be excellent.
In the past, the practice of Fangjia was extremely strict on the purity of this product. Probably it needs to reach a very high degree of purity, and the impurities must be minimal. Due to the sensitive environment of tissue culture, if there are a little more impurities, it may disturb the normal growth of cells, cause experimental deviations, or even fail.
In ancient theory, its purity often needs to reach more than 95% before it can be used for delicate matters of tissue culture. The control of impurities, starting from the selection of raw materials, must choose high-quality things, and the preparation process must be carefully handled to prevent foreign objects from being mixed in. The method of frying and purifying, step by step, strives to remove the barren and store the cyanine, so that it is pure and flawless, in order to meet the high requirements of tissue culture for the purity of the drug, to ensure the smooth growth of cells in a suitable environment, and the experimental results are accurate and reliable.
N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Tissue Culture Tested How to store
N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid), tested by tissue culture, its storage method is essential. This substance should be placed in a cool, dry and well-ventilated place. Do not expose to strong light, because light can easily change its properties and damage its effectiveness. The temperature should also be paid attention to, and it should be controlled in a moderate range, about 2-8 ° C, so that its stability can be maintained.
Furthermore, the storage place should be kept away from fire and heat sources to prevent accidents. At the same time, it needs to be placed separately from oxidizing agents, acids, bases and other substances. Due to its active chemical properties, contact with such substances may cause chemical reactions, endanger safety, and damage its quality. Where it is stored, the label must be clear, indicating the name, specifications, storage conditions and other key information for access and management. In this way, the substance must be properly preserved so that it can play its due role in subsequent tissue culture and other applications.
N- (2-Hydroxyethyl) Piperazine-N- (2-Ethanesulfonic Acid), Tissue Culture Tested What are the advantages of other congeneric products
N- (2-hydroxyethyl) piperazine-N- (2-ethanesulfonic acid), namely HEPES, has been tested in tissue culture. Compared with other congeneric products, it has many advantages.
HEPES has good buffering ability and can effectively maintain the pH stability of the solution over a wide pH range. Like an ancient good general, it can hold its ground firmly, no matter how the external acid and base changes are disturbed, it can stabilize the pH of the system as before, creating a stable environment for cell culture, just like building a strong barrier for cells to protect them from large fluctuations in pH.
Its biocompatibility is very good, and it is almost non-toxic and irritating to cells. Like a humble gentleman, treat each other gently and do not disturb the normal growth and metabolism of cells. In the microscopic world of cell culture, it does not destroy the inherent ecology of cells, so that cells can grow and multiply safely and maintain normal physiological functions.
Chemical stability, not easy to react with other substances in the system. Like a calm old man, do not participate in disputes at will to ensure that all components in the cell culture system perform their duties and do not cause chaos. It can also be stable during high temperature autoclaving, which facilitates the cell culture process. It can be sterilized together with other reagents, eliminating many complicated steps.
And it is easily soluble in water, can be quickly prepared into the required concentration solution. Like smart water, it blends seamlessly with water, saving time and energy for experimental operations and enabling experimenters to carry out cell culture-related work more efficiently.