N - (2-Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS

Lingxian Chemical

Specifications

HS Code	404299
Chemical Formula	C9H20N2O4S
Molecular Weight	252.33
Appearance	White to off - white crystalline powder
Pka At 25 C	8.0 - 8.2
Ph Range For Buffering	7.3 - 8.7
Solubility	Soluble in water
Melting Point	~235 - 238°C
Buffering Capacity	Good buffering capacity in its effective pH range
Stability	Stable under normal conditions
Compatibility	Compatible with many biological molecules

Packing & Storage

Packing	500g of N-(2 - Hydroxyethyl) Piperazine N'-3-Propanesulfonic Acid EPPS in a sealed pouch.
Storage	EPPS (N-(2 - Hydroxyethyl)Piperazine N'-3 - Propanesulfonic Acid) should be stored in a cool, dry place. Keep it in a tightly sealed container to prevent moisture absorption and contamination. Avoid storing near reactive substances. Store at room temperature preferably in a location away from direct sunlight and heat sources to maintain its chemical integrity.
Shipping	EPPS (N-(2 - Hydroxyethyl)Piperazine - N'-3 - Propanesulfonic Acid) is shipped in well - sealed containers. It's packaged to prevent moisture and contamination during transit, following strict chemical shipping regulations.

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N - (2-Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS

General Information

Historical Development

Guanfu EPPS This product, its name is N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid. Tracing its origin, at the beginning of the chemical industry, in the exploration of exquisite reagents, unremitting research. At that time, the technology was not as prosperous as it is today, but everyone relied on their determination to study the physical properties and explore the preparation method.
After years of hard work, this good product was obtained. EPPS first appeared and made a name for itself in biochemical research. In the buffer system, it has excellent performance and can stabilize the degree of acid and base, which is the cornerstone of many experiments. With the passage of time, scientific research has advanced, and EPPS has been widely used. It has made outstanding achievements in cell culture, protein analysis, and other fields, helping the academic community to repeatedly solve problems and promoting the vigorous progress of biochemistry.

Product Overview

Nowadays, there is a substance called N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid, referred to as EPPS. This is a product of fine chemicals and has a wide range of uses. Its mild nature can be used as a buffer in biochemical research and other fields to maintain the stability of the pH of the system.
Looking at its structure, it has both the skeleton of piperazine and the hydroxyethyl and propanesulfonic acid groups. This unique structure gives it good buffering properties. When preparing, the reaction conditions need to be carefully controlled to ensure good quality. EPPS is soluble in water and is compatible with many reagents, which provides convenience for experimental operation. It is an indispensable product in chemical research and is useful in many aspects such as biochemistry and medicine.

Physical & Chemical Properties

EPPS, that is, N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid is also. Its properties, with acid-base buffering ability. In biochemical experiments, it is often used as a buffer. Looking at its physical properties, it is a white crystalline powder at room temperature, easily soluble in water, and colorless and transparent. Regarding its chemical properties, it can stabilize the solution of acid and alkali in a suitable pH range. Because of this structure, there are protonated and deprotonated groups, which change according to the pH of the environment and move in balance to maintain pH stability. And it reacts less with a variety of reagents and does not disturb the experimental process. This characteristic makes EPPS widely used in biochemistry and molecular biology experiments, and is also an important reagent for researchers.

Technical Specifications & Labeling

EPPS, that is, N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid. Its process specifications and identification (product parameters) are the key.
As far as process specifications are concerned, the synthesis process needs to be strictly controlled, the raw materials used must be pure, and the reaction conditions such as temperature, pressure, and duration need to be precisely controlled to ensure that the reaction is complete and the purity of the product is improved. As for the logo, the product label should be named EPPS, listing the molecular formula and molecular weight in detail, clearly marking the parameters such as purity, water content, and pH range, and clearly indicating the storage conditions and precautions in a prominent place in the package to prevent deterioration and ensure that the product quality is the same, so that it can be used for scientific research.

Preparation Method

The method of making EPPS requires first to clarify its raw materials. Take 2-hydroxyethylpiperazine and 3-propanesulfonic acid as the main ones. The preparation process first makes the two meet, and the temperature is controlled to promote the reaction. The reaction process, observe the change of its color and properties, and proceed in sequence. After the reaction is completed, it needs to be refined and purified. Recrystallization and chromatography can be used to remove its impurities and keep pure EPPS. This refining method is related to the purity of the product and cannot be ignored. And in the whole preparation process, the reaction rate, temperature, pressure, and stability mechanism can be controlled to obtain high-quality EPPS products, which can be used for various biochemical studies without mistaking their effectiveness.

Chemical Reactions & Modifications

Taste the wonders of chemical industry, it is related to the change of matter. On N - (2 - Hydroxyethyl) Piperazine N '- 3 - Propanesulfonic Acid (EPPS). Its chemical reaction is like the interaction of yin and yang in heaven and earth, and new substances are formed.
The reaction of EPPS has something to study. Its molecular structure is exquisite, and in the reaction, functional groups interact, such as dancers and rhymes, with orderly rhythm. Hydroxyl groups, sulfonic acid groups, etc., each show their own abilities, or affinity or sparsity, causing the reaction to proceed according to specific rules.
As for variability, EPPS is like a wise man's change in different situations. The difference in temperature and pH can make the reaction path easier, and the products are also different. This variability expands a broad field for chemical applications and can be modulated on demand to meet diverse requirements. It is widely used in biochemical, pharmaceutical and other industries. It is also a treasure of chemical industry.

Synonyms & Product Names

EPPS, that is, N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid. Its name is different, and it is also known. This substance has a wide range of uses, and is often used as a buffer in biochemical research. Looking at its characteristics, it has good buffering ability and can stabilize the acid and base of the system. It is the same as all buffers, but it also has its uniqueness. EPPS helps researchers control conditions in many experimental scenarios and makes the results more accurate. With its unique chemical structure, it acts delicately between solutions. Therefore, in the field of biochemistry, EPPS is called unique and excellent in nature. It is a weapon for scientific research, helping to explore the secrets of life and uncover the secrets of chemistry.

Safety & Operational Standards

The name of the chemical substance today is "N - (2 - Hydroxyethyl) Piperazine N '- 3 - Propanesulfonic Acid EPPS", which is related to the safety and operation specifications of this product. It should be explained in detail.
EPPS This product must abide by the specifications when operating. The operator needs to be professionally trained and familiar with the operation process. Operate in a well-ventilated place to avoid dust. And when operating, wear suitable protective clothing, goggles and protective gloves to prevent it from coming into contact with the skin and eyes.
As for safety, although EPPS has specific chemical properties, it can be kept safe if handled properly. If you accidentally touch the skin, you should quickly rinse it with plenty of water and seek medical treatment if necessary. If it enters the eye, you should also rinse it with water immediately and then seek medical attention. When storing, it should be placed in a cool, dry and well-ventilated place, away from fire and heat sources, and stored separately from oxidants. Do not mix.
Although EPPS is a chemical substance, it can be used well in accordance with safety and operating standards, and it can be used without fear of safety. It can play its due role in chemical research and other fields.

Application Area

EPPS, N - (2 -hydroxyethyl) piperazine N '-3 -propanesulfonic acid is also. Its application field is wide, especially important in biochemical research.
In the study of proteins and enzymes, EPPS can create a stable acid-base environment, so that proteins and enzymes maintain their inherent activity and structure. Because of its good buffering performance, it can precisely regulate the pH value of the reaction system, making the experimental results more reliable.
In the field of cell culture, EPPS can ensure the stability of acid and base in the microenvironment of cell growth, which is conducive to normal metabolism and proliferation of cells. Provide suitable pH conditions for cells, just like fertile soil breeding, to ensure healthy cell growth.
EPPS is indispensable in many biochemical experiments and biomedical research, and has made great contributions to scientific research progress.

Research & Development

In recent years, researchers have paid attention to N - (2 - Hydroxyethyl) Piperazine N '- 3 - Propanesulfonic Acid, that is, EPPS. EPPS has a wide range of uses in the field of biochemistry, can be used as a buffer reagent to maintain the acid-base balance of the system, and is beneficial to the study of proteins and enzymes.
We have worked hard to study the method of its preparation, and strive to improve. At first, we tried all kinds of paths, or the raw materials were rare, or the steps were complicated, and the yield was not satisfactory. After repeated investigation, the process was improved, and the raw materials were easy to purchase and cheap, the process was simple and efficient, and the yield gradually increased.
Looking at its development, the need for EPPS is increasing day by day, and the scope of application is also expanding. Our generation should make unremitting efforts to study, optimize production, improve quality, and make every effort for the development of chemical industry and the progress of scientific research.

Toxicity Research

Nowadays, there is a substance named N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid, which is called EPPS. As a chemical researcher, I often consider its toxicity studies. EPPS is a common reagent in biochemical experiments.
To understand its toxicity, extensive data collection is required and detailed testing is required. Observe its reaction in different environments and different organisms. However, toxicity research is not done overnight. It is necessary to follow the scientific method to observe its effect on the physiological and biochemical indicators of organisms.
Or in cell experiments, to explore its effect on cell growth and metabolism; or in animal experiments, to observe its change in organ function and behavior. Only with prudence and careful study can we obtain the true appearance of EPPS toxicity and provide evidence for its safe use and rational application.

Future Prospects

Now N - (2 -hydroxyethyl) piperazine N '-3 -propanesulfonic acid (EPPS) is a chemical product with promising prospects. Its use in the field of biochemical research is becoming more and more extensive. In the future, scientific research progress will urge its application to expand.
Or it can be used as a buffer reagent in more accurate biological experiments to make the experimental conditions stable and controllable, and help the research results become more accurate. Or in the process of new drug development, with its unique chemistry, it participates in key reactions and becomes the cornerstone of innovative drugs.
We imagine that EPPS will be like a star, shining brightly in the future of scientific research, helping to explore the unknown, opening up new chapters, and contributing to human well-being.

Frequently Asked Questions

What is N- (2 - Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS?

N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, or EPPS, is a very important biochemical reagent. It is widely used in the field of biochemical research and is often used as a buffer.
Looking at this EPPS, it has many unique properties. From the chemical structure, it contains specific functional groups, which endows it with good buffering properties. In many biochemical experiments, it is essential to maintain a stable pH environment, and EPPS can play a key role here. It can effectively adjust the pH of the solution and keep the pH value in a relatively constant range.
In the research experiments of proteins and enzymes, EPPS buffer system is frequently used. The activity of proteins and enzymes is highly susceptible to changes in environmental pH, and the stable pH conditions created by EPPS help to ensure that proteins and enzymes maintain their normal structure and function, and avoid their denaturation or inactivation due to pH fluctuations.
In addition, in experimental scenarios such as cell culture, EPPS is also indispensable. Cell growth and metabolism are extremely sensitive to environmental pH, and a suitable buffer system provides a stable acid-base environment for cell growth. EPPS can meet this need and help cells grow and multiply healthily under suitable pH conditions. EPPS, with its excellent buffering properties, plays an important role in many research fields such as biochemistry and molecular biology, providing a solid guarantee for the smooth development of various experiments and greatly promoting the progress and development of related scientific research.

What are the main uses of N- (2 - Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS?

N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid (EPPS) has a wide range of uses. In biochemical research, it is often used as a buffer. Because it has a suitable acid-base buffer range, about pH 7.3-8.7, it can stabilize the pH of the solution, so it is indispensable in many biochemical reaction systems.
In the field of protein research, EPPS can ensure the stability of the structure and function of proteins. Proteins can easily cause structural alteration and functional impairment in an uncomfortable acid-base environment. EPPS can control the pH of the system, making the protein in a good condition, which is conducive to the study of its characteristics, activities and interactions.
In the process of cell culture, EPPS also has great achievements. Cell growth requires a suitable acid-base environment. EPPS can maintain the pH stability of the culture medium and prevent it from fluctuating greatly due to the accumulation of cell metabolites, so as to help the normal growth and proliferation of cells, providing a guarantee for the smooth cell culture experiment.
In the enzymatic reaction, EPPS acts as a buffer medium, which can adjust the pH of the reaction environment and keep the enzyme in the optimum pH conditions to develop its high-efficiency catalytic activity, which is of great significance for the study of the kinetics and mechanism of action of the enzyme.
In addition, EPPS can stabilize the pH in molecular biology experiments, such as nucleic acid extraction and amplification. It can also protect the integrity of the nucleic acid structure and ensure the reliability of the experimental results.

What are the physicochemical properties of N- (2 - Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS?

N- (2-hydroxyethyl) piperazine-N '-3-propanesulfonic acid, or EPPS, is a commonly used zwitterionic buffer for biochemical research. Its physicochemical properties are as follows:
EPPS is mostly white crystalline powder in appearance, stable in properties, easy to store and use. It has good solubility in water and can quickly dissolve to form a uniform solution. This property makes it extremely convenient for buffer preparation and can quickly reach the desired concentration.
EPPS has excellent buffering properties and can effectively maintain the pH value of the solution within a specific pH range. The effective buffer range is about pH 7.3-8.7, which is suitable for many biochemical reactions and experimental needs. Due to the fact that the pH value of most organisms is near this range, EPPS can simulate the acid-base environment in organisms, ensure the stability of biomolecular structure and activity, and provide suitable pH conditions for related research.
EPPS has a relatively high melting point, generally at 231-235 ° C. The higher melting point indicates that its intermolecular force is strong and the structure is stable. It is not easy to decompose or phase change due to temperature changes under conventional experimental conditions, ensuring the stability of the experimental system. In addition, EPPS is chemically stable, does not easily react with common chemical reagents, and can maintain its own structure and properties in different chemical environments. It can coexist with a variety of salts, proteins, nucleic acids, and other biomolecules without affecting its performance, providing significant advantages in the study of complex biochemical systems.

N- (2 - Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS What are the precautions in the experimental operation?

N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid (EPPS) requires several precautions during experimental operation.
First, the dissolution of EPPS is crucial. Because its solubility is related to many factors, water temperature is one of them. Generally speaking, dissolving EPPS in warm water can make its dissolution smoother. If the water temperature is too low, the dissolution rate of EPPS is slow, or the dissolution may be insufficient; if the water temperature is too high, it may affect its chemical properties, so the water temperature must be accurately controlled when dissolving.
Furthermore, the adjustment of pH value should not be underestimated. As a buffer, EPPS has a specific buffer range, generally between pH 7.3 and 8.7. During the experimental operation, the pH value of the system needs to be carefully adjusted according to the specific needs of the experiment. During the adjustment process, the concentration and amount of reagents used need to be carefully controlled to prevent the pH value from deviating from the expected range and causing the experimental results to deviate.
In addition, the stability of the EPPS solution also needs to be paid attention to. It deteriorates in the air or due to factors such as carbon dioxide, so the prepared solution should be used as soon as possible and stored properly. If it is not stored properly, the solution may breed microorganisms or undergo chemical reactions, which will make the EPPS ineffective and affect the experimental results.
At the same time, the compatibility of EPPS with other reagents cannot be ignored. Before mixing EPPS with other chemical reagents, it is clear whether the two will react. Some reagents may produce precipitation, discoloration, etc. with EPPS, which interferes with experimental observation and result analysis. Therefore, a pre-experiment should be carried out before the experiment to confirm the compatibility between the reagents.
Finally, the experimental operation must follow the specifications. When using EPPS, the utensils used should be clean and dry to prevent impurities from mixing. After the operation is completed, properly dispose of the remaining reagents and must not be discarded at will to avoid pollution to the environment.

What are the advantages of N- (2 - Hydroxyethyl) Piperazine N '-3-Propanesulfonic Acid EPPS over other similar reagents?

N- (2-hydroxyethyl) piperazine N '-3-propanesulfonic acid (EPPS) is a commonly used buffer in biochemical research, and has many advantages over other similar reagents.
First, EPPS has a specific pH buffer range, about pH 7.3-8.7. In many biochemical reactions and biological systems, this pH range is crucial. Many biological macromolecules, such as proteins and enzymes, can maintain stable structure and activity in this range. Other similar reagents have different buffer ranges. If this specific range is required in application scenarios, EPPS is more suitable.
Second, EPPS is chemically stable. Under common experimental conditions, it is not easy to react with other chemical substances, which can ensure the stability of the buffer system and make the experimental results more reliable. Some similar reagents may deteriorate due to structural differences, poor stability, and easy to be affected by environmental factors, interfering with the experiment.
Third, EPPS has low toxicity to biological systems. In biological experiments such as cell culture and enzyme activity determination, it will not have significant adverse effects on biological samples and can truthfully reflect the reactions and processes in biological systems. Some similar reagents may be toxic to organisms due to their special groups, limiting their use.
Fourth, EPPS has good solubility, which can be quickly dissolved in water or some organic solvents, which is conducive to the preparation of buffer during experimental operation, which can effectively save time and energy and ensure the efficient development of experiments. In contrast, some similar reagents have poor solubility and complicated dissolution process, which affects the experimental efficiency.