What is the chemical structure of N-Cyclohexyl-3-Aminopropanesulfonic Acid Caps?
N-Cyclohexyl-3-Aminopropanesulfonic Acid is N-cyclohexyl-3-aminopropanesulfonic acid, often abbreviated as CAPS. Its chemical structure is as follows:
First, the molecule contains a cyclohexyl group. Cyclohexyl is a six-membered cyclic structure, and six carbon atoms are connected to each other by a single bond to form a stable cyclic skeleton.
On a carbon atom of the cyclohexyl group, an aminopropanesulfonic acid group is connected. Among them, the amino group (-NH2O) is a basic functional group, which has a pair of lone pairs of electrons and can accept protons and thus exhibits alkalinity. Connected to the amino group is a propyl group (-CH -2 CH -2 -), which acts as a linking group to connect the amino group to the sulfonic acid group.
Sulfonic acid group (-SO 😉 H) is another important functional group of this molecule. The sulfonic acid group has strong acidity and can completely ionize hydrogen ions in aqueous solution, making the whole compound exhibit a certain acid-base quality.
As a whole, the chemical structure of N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) fuses the cyclic structure, basic amino group and acidic sulfonic acid group. This unique structure endows it with specific application properties in biochemical and chemical research, and is often used in the construction of buffer systems and other fields.
What are the main uses of N-Cyclohexyl-3-Aminopropanesulfonic Acid Caps?
N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) has a wide range of uses. In the field of biochemical research, it is often used as a buffer. Because of its good buffering properties, it can maintain the stability of the pH of the solution in a specific pH range, which is crucial for many biochemical reactions that require precise control of pH, such as enzymatic reactions. Enzymes can exhibit the best activity in a suitable pH environment. CAPS can create such an environment, so that the enzymatic reaction can proceed smoothly without being disturbed by pH fluctuations.
It also appears frequently in molecular biology experiments. In the nucleic acid extraction and purification process, it can regulate the pH of the reaction system to ensure the stability of the nucleic acid structure and prevent acid-base damage. In addition, in protein electrophoresis experiments, adding CAPS to the buffer can maintain a stable pH environment, ensure the stability of protein migration rate in the electric field, and effectively separate proteins according to their own charge and molecular weight differences, helping researchers accurately analyze protein properties.
In the field of cell culture, CAPS also contributes. Cell growth requires strict environmental pH, which can adjust the pH of cell culture medium, create a suitable living environment for cells, promote normal cell growth and proliferation, and prevent abnormal cell physiological function and even death due to pH discomfort.
In the process of drug development, drug synthesis reactions or drug stability studies, specific pH conditions are often required. As a buffer, CAPS can precisely regulate the pH of the reaction system or drug preparation to ensure smooth drug synthesis and good drug stability, which is of great significance for improving drug quality and efficacy.
What is the purity standard for N-Cyclohexyl-3-Aminopropanesulfonic Acid Caps?
N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) is widely used in biochemical experiments, and its purity standard is the key. According to "Tiangong Kaiwu", the material is pure to get good results, and the same is true for CAPS.
The purity standard of CAPS is the first chemical purity. High-purity CAPS has very few chemical impurities. If it is detected by high-performance liquid chromatography (HPLC), the proportion of the main peak area should reach a very high proportion, usually more than 99%, to ensure that its chemical composition is pure and there is no interference from too many heterogeneous peaks. If there are many impurities, it will be like weeds in a good field in the experiment, which will disrupt the order of the experiment and cause deviations in the results.
The second is the moisture content. Moisture has a great influence on biochemical reagents, and CAPS is no exception. Its moisture content should be strictly controlled, generally determined by Karl Fischer's method, and should be lower than a specific value, such as 0.5%. Excessive moisture, like the decaying beetle of wood, can cause deliquescence and deterioration of CAPS, affecting its stability and reactivity.
Furthermore, the pH (pH) related index. Although CAPS is a buffer, its own pH needs to be stable and within a specific range. At a specific concentration, its pH fluctuation should be minimal. If a solution of a certain concentration is prepared, the pH value should be within a certain precise range. Otherwise, if the boat goes against the water, it will affect its buffering performance and make the pH of the experimental system unstable.
In addition, the burning residue is also an important purity consideration. After being burned at high temperature, the residual material should be minimal, and it is usually stipulated that the burning residue should not exceed a certain proportion, such as 0.1%. If this residue is too much, it will enter the good device, which will contaminate the experimental system and interfere with the experimental process and results.
In short, to obtain high-quality CAPS, all purity indicators must meet strict standards, so that in biochemical experiments, it can be as smooth as a good horse and provide reliable protection for scientific research.
N-Cyclohexyl-3-Aminopropanesulfonic precautions when storing Acid Caps
When storing N-cyclohexyl-3-aminopropanesulfonic acid (Caps), pay attention to the following things.
First, temperature is very important. Caps should be stored in a cool place to avoid high temperature. High temperature can easily cause its chemical properties to change, or accelerate decomposition. Generally speaking, it is better to refrigerate at 2-8 ° C, so as to maintain its stability and prolong the service life.
Second, humidity also needs to be concerned. It should be placed in a dry environment to prevent moisture. Moisture will make Caps agglomerate, affecting its purity and use effect. There should be no water source in the storage place, and the humidity should be controlled at 40% - 60%.
Third, shading is also the key. Caps are sensitive to light, and light may cause them to undergo photochemical reactions, resulting in deterioration. Therefore, it should be stored in a brown bottle or opaque container and placed in a dark place.
Fourth, sealed storage is indispensable. Contact with air, or cause Caps to oxidize, or absorb impurities in the air. Be sure to seal tightly after use to prevent external factors from interfering.
Fifth, separate storage should not be ignored. It should not be co-stored with strong oxidants, strong acids, strong bases and other substances. Because of its active chemical properties, contact with it or react violently, endangering safety and reducing quality. When
Caps are stored, they should be properly disposed of in terms of temperature, humidity, light, sealing and separate storage to ensure their stability for subsequent use.
N-Cyclohexyl-3-Aminopropanesulfonic the market price range of Acid Caps
N-cyclohexyl-3-aminopropanesulfonic acid (Caps) has a wide range of uses in biochemical research, and its market price range varies greatly due to quality, purity and supply channels. Looking at the method of "Tiangong Kaiwu" in the past, although it does not directly discuss the price of this product, when it comes to the price of the product, it often involves various factors, which can be compared.
In today's city, if it is an ordinary purity Caps for general experimental needs, the price per hundred grams may be between tens of yuan and more than 100 yuan. This is because the process is mature and the output is quite abundant, so it can maintain a relatively easy price.
However, if it is a high purity, suitable for high-end scientific research or special industrial processes, the price will rise significantly. This is because the purification technique is complicated and the cost is greatly increased, so the price is high.
Furthermore, the purchase volume is also a key variable in the price. Bulk purchases, due to economies of scale, the unit price is discounted every time. Small retail purchases, the price is often high.
Overall, the market price of Caps varies according to purity, purchase volume and supply and demand conditions, and its price per hundred grams fluctuates in the range of tens to hundreds, and even hundreds.