N. N-Bis (2-Hydroxyethyl) -2-Aminoethanesulfonic Acid Bes

N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes) is a commonly used buffer in biochemical research. Its main uses cover a wide range of fields.
In biochemistry and molecular biology experiments, Bes is often used to maintain a stable pH environment. Many biochemical reactions are extremely sensitive to pH, and subtle pH changes may affect the reaction rate, enzyme activity, and even the structure and function of biological macromolecules. Bes has excellent buffering ability, which can effectively resist pH fluctuations caused by the increase and decrease of protons during the reaction process, ensuring that the experimental system is in a suitable pH range, so that various biochemical reactions can proceed normally and orderly.
In the field of cell culture, Bes also plays a key role. Cell growth requires a strict pH value of the environment, and a stable pH environment is a necessary condition for cell survival, proliferation and normal physiological function maintenance. Adding Bes to the cell culture medium can precisely regulate the pH value of the culture medium, creating a stable acid-base environment for cells like in the body, helping cells grow and multiply healthily, and improving the success rate and quality of cell culture.
In addition, Bes is also indispensable in protein and nucleic acid research. During the separation, purification and analysis of proteins and nucleic acids, a suitable pH environment is essential to maintain their natural structure and activity. Bes can provide a stable buffer system to prevent the denaturation of proteins and nucleic acids due to abnormal pH, ensuring the accuracy and reliability of research results.
In summary, N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes), with its unique buffer properties, plays a pivotal role in many key fields such as biochemical research, cell culture and biomacromolecular research, and contributes significantly to the development of life science research.

N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes) is widely used in the fields of biochemical research, pharmaceutical research and development, food industry, and agricultural production.
In the field of biochemical research, Bes is an excellent buffer. Because of its specific pH buffer range, it can maintain the stability of the acid and base environment in organisms. For example, in the study of biological macromolecules such as proteins and enzymes, it can create a suitable pH to ensure the structure and activity of biomolecules. In protein crystallization experiments, precise adjustment of solution pH helps to obtain high-quality protein crystals and lays the foundation for analyzing their structures.
In the field of pharmaceutical research and development, Bes also plays a key role. In pharmaceutical preparations, it can be used as a buffer component to improve drug stability and solubility. When preparing injections, adjust the pH to an appropriate range to reduce the irritation of drugs to the body and enhance the efficacy of drugs. In some eye drops formulations, Bes is added to maintain the pH balance in the eye and reduce the adverse effects on the eyes.
In the food industry, Bes can be used as a food additive. It can adjust the pH value of food, prevent corrosion and keep fresh. In some acidic beverages, stabilize the pH, prevent the growth of microorganisms, and prolong the shelf life. In baked goods, adjust the pH of dough to improve the baking quality.
In the field of agricultural production, Bes can adjust the pH of soil. In some areas, the soil is over-acidic or over-alkaline, which affects the growth of crops. Applying Bes-containing preparations can improve the soil environment, create favorable conditions for crop growth, and then improve crop yield and quality.
In short, N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes) has important applications in many fields and is of great significance to promoting the development of various industries.

N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes) is a commonly used zwitterionic buffer in biochemical research. Its physicochemical properties are quite unique, and it is crucial for the stability and maintenance of biochemical experimental systems.
Bes bears the brunt, and Bes has good water solubility. It can be quickly dissolved in water to form a homogeneous solution, which makes it extremely convenient to prepare buffers. Because it can quickly and evenly disperse in solvents, it ensures uniform distribution of buffer components in the experimental system, laying a foundation for subsequent biochemical reactions to stabilize the environment.
Secondly, the buffer range of Bes is quite critical. Its effective buffering pH range is about 6.4-7.8, which is similar to the environmental pH of many organisms. The structure and function of most enzymatic reactions, proteins and nucleic acids in organisms are extremely sensitive to pH value. Bes can effectively resist pH changes caused by the addition of a small amount of acid or base in this specific pH range, just like a loyal guard guarding the acid-base balance of biological systems, maintaining the normal conformation and activity of biomolecules.
Furthermore, the chemical stability of Bes is quite good. Under common experimental conditions, it is not easily affected by chemical reactions such as oxidation and hydrolysis, and can maintain its own structure and properties stable for a long time. Even in harsh environments such as high temperature and high humidity, it can maintain stable buffering performance, which greatly guarantees the repeatability and reliability of experimental results.
In addition, Bes has good compatibility with biomacromolecules. In the buffer system, it interacts weakly with proteins, nucleic acids and other biomacromolecules, and rarely interferes with the normal physiological functions and chemical reactions of biomacromolecules. This property allows researchers to carry out experiments in close to the real environment in vivo, and the results obtained are more biologically significant and reference value.
All in all, N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes) occupies a pivotal position in the field of biochemical research due to its unique physicochemical properties, providing a powerful tool for researchers to deeply explore the mysteries of life.

The methods for preparing N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (Bes) are as follows:
First, ethanolamine and 2-chloroethanesulfonic acid are used as materials. Take an appropriate amount of ethanolamine first, place it in the reactor, slowly add 2-chloroethanesulfonic acid, and adjust the reaction temperature to a suitable range, such as 50 to 80 degrees Celsius. Because the reaction between the two will generate heat, pay attention to temperature control to prevent side reactions from being caused by excessive temperature. And an appropriate amount of acid binding agent, such as sodium carbonate, can be added to remove the hydrogen chloride generated by the reaction and make the reaction go in the positive direction. After several hours, the reaction process was monitored by thin-layer chromatography. When the raw material point was almost gone, the reaction was completed. After that, the reaction liquid was cooled and recrystallized with an appropriate solvent, such as ethanol-water mixed solvent, to obtain pure Bes.
Second, 2-aminoethanol is used with ethylene oxide and sodium bisulfite as materials. First, 2-aminoethanol is reacted with ethylene oxide. This step is easy to progress under alkaline conditions. A little sodium hydroxide can be added as a catalyst, and the temperature is controlled at 30 to 60 degrees Celsius. Ethylene oxide is ring-opened and 2-aminoethanol is added to obtain an intermediate product. Next, the intermediate product is reacted with sodium bisulfite, and the temperature can be slightly raised to 70 to 90 degrees Celsius, so that the terminal hydroxyl group of the intermediate product is sulfonated with sodium bisulfite. After the reaction is completed, the unreacted raw materials and impurities are removed through reduced pressure distillation, ion exchange resin purification and other steps, and Bes can also be obtained.
Third, N- (2-hydroxyethyl) -2-aminoacetonitrile is used as the starting material. First, N- (2-hydroxyethyl) -2-aminoacetonitrile is heated with an aqueous solution of sulfuric acid, and hydrolyzed at a temperature of about 100 to 120 degrees Celsius to convert the cyano group into a carboxyl group. After the reaction is completed, it is cooled and neutralized to neutral in sodium hydroxide solution. After concentration and crystallization, the crude Bes product is obtained, and then refined by column chromatography or recrystallization to obtain high purity Bes.

When using N, N-bis (2-hydroxyethyl) - 2-aminoethanesulfonic acid (BES), many key matters need to be paid attention to.
First, the dissolution of this reagent is quite important. BES has good solubility in water, but the dissolution process may sometimes be slightly slower. If you want to accelerate the dissolution, you can heat it moderately and add stirring. However, when heating, you must pay attention to the temperature not being too high to prevent BES from decomposing and damaging its chemical properties. Generally speaking, it is appropriate to control the temperature between 30-50 ° C, and the stirring speed should not be too fast, so as not to produce too much foam and affect the operation.
Second, pH adjustment needs to be cautious. BES is often used as a buffer, and its buffer range is between pH 6.4 and 7.8. When using, the pH value should be precisely adjusted according to the specific experimental needs. During the adjustment process, acid or alkali solutions should be added dropwise slowly, while continuous stirring and real-time monitoring with the help of a pH meter to ensure that the pH value is stable within the target range. If the adjustment speed is too fast, it is easy to cause the pH value to exceed expectations, thus affecting the experimental results.
Third, the storage conditions should not be ignored. BES should be stored in a dry, cool and well-ventilated place, away from heat sources and strong light. If not properly stored, such as in a humid environment, BES may absorb moisture and agglomerate, which will affect its purity and use effect. In addition, it is also necessary to pay attention to its shelf life. Expired BES or has deteriorated, so it must not be used for experiments.
Fourth, in terms of safety, although BES is relatively low toxic, it is still necessary to avoid direct contact with the skin and eyes during operation. If it is accidentally contacted, it should be rinsed with plenty of water immediately and seek medical attention in time if necessary. Operating in a well-ventilated environment can also reduce the risk of inhalation and ensure the safety of experimenters.
In short, when using BES, every link from dissolution, pH adjustment, storage to safety protection needs to be properly handled to ensure the smooth progress of the experiment and obtain accurate and reliable results.