What is the chemical structure of N-Tris (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid?
N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, its chemical properties are as follows:
This compound contains a monoethanesulfonic acid group (- CH 2O CH 2O SO-H), and a trimethylmethyl (- CH 2O OH) in the nitrogen atomic phase. The carbon atom in the nitrogen atom's ethanesulfonic acid group forms the skeleton of the whole molecule. Its chemical formula can be C H 5NO S.
Trimethylmethyl gives the molecule good water-based water-based, so that it has a certain solubility and qualitative properties in aqueous solution. The ethanesulfonic acid group provides acidic properties, enabling it to dissolve in a certain piece, and shows performance. This compound is often used in biological chemistry because it can effectively maintain the acidity of the solution at a specific pH, ensuring the stability and function of biological macromolecules (such as proteins, nucleic acids, etc.). The interaction of each group in the compound jointly determines the chemical and physical properties of the compound in different environments.
Common Uses of N-Tris (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid
N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, commonly known as TES, is a commonly used amphoteric buffer in biochemical research. It has a wide range of uses in biochemical experiments, as follows:
First, it is crucial in the study of enzyme activity. Various enzymatic reactions are extremely sensitive to environmental pH, and TES can create and maintain a specific pH environment, so that enzymes are in the best active state. For example, in some protein enzyme activity measurement experiments that require precise pH conditions, TES buffer can ensure the pH stability of the reaction system, help researchers accurately determine enzyme activity, and clarify the mechanism of enzyme action.
Second, it is also indispensable in the field of protein research. The structure and function of proteins are closely related to the pH of the environment in which they are located. TES buffer can prevent proteins from being denatured due to pH fluctuations and maintain their natural conformation and biological activity. During the process of protein purification and crystallization, maintaining a suitable pH with the help of TES buffer can improve protein purity and crystallization success rate, laying the foundation for protein structure analysis.
Third, it has a significant effect in cell culture. Cell growth has strict requirements on environmental pH, and minor pH changes may affect cell metabolism, proliferation and even survival. TES buffer can effectively adjust the pH of cell culture medium and create a stable survival environment for cells. It is widely used in a variety of cell culture experiments in vitro to ensure normal physiological functions of cells and assist cell biology research.
Fourth, nucleic acid research is also used. Nucleic acid extraction, amplification, electrophoresis and other experimental links require a suitable pH environment. TES buffer can stabilize the pH of the reaction system, ensure the stability of nucleic acid structure, prevent nucleic acid degradation, and improve the accuracy and repeatability of experimental results. For example, when optimizing the PCR reaction system, TES buffer can be used to precisely regulate the pH to improve the amplification efficiency.
What is the suitable pH range for N-Tris (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid in biological experiments?
N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, or TES, is very important in biological experiments. The pH range of this reagent is usually between 6.8 and 8.2.
In many biological experimental scenarios, maintaining a suitable pH environment is of great significance to the stability of biomolecules and the normal advancement of biochemical reactions. Due to its unique chemical properties, TES can exhibit excellent buffering ability in a specific pH range.
When the pH is about 6.8, TES can effectively resist the interference of acidic substances, so that the pH of the system does not drop sharply; while at nearly 8.2, it can also stabilize the pH of the system in the face of the invasion of alkaline substances and avoid a sharp rise.
For example, in protein research experiments, the structure and function of proteins are extremely sensitive to the pH of the environment. If the pH deviates from the appropriate range, the protein may denature and damage its function. At this time, the use of TES as a buffer in the pH range of 6.8 to 8.2 can ensure that the protein is in a stable environment, allowing the experiment to proceed smoothly, and assisting researchers in accurately exploring protein properties.
How soluble is N-Tris (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid?
N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, its dissolution characteristics are related to many experiments and applications. This substance is easily soluble in water, just like manna melts into the earth, and can be infinitely mixed with water to form a uniform solution. Under normal temperature, in an appropriate amount of water, it can dissolve quickly, just like ice and snow melt in the warm sun.
Its dissolution process is smooth and orderly, not as abrupt as a strong wind and rain. When it is slowly added to water, with a little stirring, it is like a boat wave, gradually dispersing and dissolving. Because its molecular structure is rich in hydrophilic groups, it is like countless small hands, tightly embracing water molecules, so it shows good water solubility.
However, its solubility in organic solvents is very different. Compared with the affinity of water, organic solvents such as alcohols and ethers have a lower degree of acceptance. In these organic solvents, it is often difficult to dissolve as easily as in water, as if it is in a foreign land, and it is difficult to integrate into it. This is because the molecular properties of organic solvents are quite different from those of water, and it cannot form an effective effect with the hydrophilic groups of the substance, resulting in limited solubility. With such solubility characteristics, in actual operation, the experimenter needs to carefully select the solvent according to specific needs in order to make the substance work best.
What are the advantages of N-Tris (Hydroxymethyl) Methyl-2-Aminoethanesulfonic Acid over other buffer reagents?
N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, commonly known as TES, has obvious advantages compared with other buffer reagents.
The first is that the acid-base range is suitable. The effective buffer range of TES is about pH 7.5-8.5. In many biochemical and molecular biology experiments, this range is suitable for the stability and activity of many biological macromolecules. If protein research, multi-proteins can maintain their natural conformation and function in this pH range, and TES can create such a stable acid-base environment, which is much more beneficial than those whose buffer range is not suitable.
The second is that the chemical stability is good. Under common experimental conditions, TES is not easy to react with metal ions, oxidants, reducing agents, etc. Taking nucleic acid experiments as an example, metal ions often cause nucleic acid degradation, but the buffer system constructed by TES can resist the interference of metal ions. It is a buffer that is easier to react with other substances, which can ensure the integrity of nucleic acid, and the experimental results are more reliable.
Furthermore, it has excellent biocompatibility. TES is almost non-toxic and non-interfering to most biological systems. In cell culture experiments, TES is used as a buffer, which does not affect cell growth, metabolism and differentiation. Compared with cytotoxic buffer reagents, it can more accurately reflect the physiological state of cells, and the authenticity and scientificity of experimental data can be improved.
In addition, the solubility is good. TES has good solubility in water and common organic solvents. It is convenient and efficient to prepare buffer solutions, and can quickly achieve a uniform and stable state. It saves a lot of tediousness for experiments and is indispensable for modern experiments that pursue efficiency.