What are the common applications of N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt?
N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt, that is, N- (tris (hydroxymethyl) methyl) -2-aminoethanesulfonate sodium salt, often referred to as TES sodium salt. This substance has a wide range of uses and is frequently used in the field of biochemical research.
First, it plays an important role in biological buffer systems. Many biochemical reactions in organisms are extremely sensitive to environmental pH levels, and slight changes may affect the reaction process and enzyme activity. Due to its good buffering ability, TES sodium salt can maintain the pH stability of the solution within a specific pH range. It is like a strong fortress, escorting many experiments such as enzymatic reactions, protein crystallization and DNA research, ensuring that the pH of the experimental system is in an appropriate range, so that the reaction can proceed smoothly.
Second, in the field of cell culture, when cells are cultured in vitro, the environment requirements are strict, and pH stability is one of the key factors. TES sodium salt can be integrated into cell culture medium to create a stable acid-base environment, like a warm home, providing suitable conditions for cell growth and proliferation, helping cells to survive healthily, and laying a solid foundation for cell biology research.
Third, in protein research, protein properties and functions are significantly affected by environmental pH. The stable pH environment created by TES sodium salt is conducive to protein separation, purification and identification. In protein analysis technologies such as electrophoresis and chromatography, it can prevent protein denaturation due to pH fluctuations, ensure that proteins maintain their natural structure and function, and help researchers accurately analyze protein mysteries.
What are the physical properties of N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt?
N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt, that is, N- (tris (hydroxymethyl) methyl) -2-aminoethyl sulfonate sodium salt, often referred to as TES sodium salt. The physical properties of this substance are quite important, related to its application in various chemical and biological fields.
Looking at its properties, at room temperature, it is mostly white crystalline powder, which is easy to weigh, store and use. It has good stability and is not easy to decompose or deteriorate under normal environmental conditions.
In terms of solubility, TES sodium salts are easily soluble in water, which makes them rapidly and evenly dispersed in experiments and reactions in aqueous solution systems, which is conducive to the development of various chemical reactions and biological experiments. The dissolution process is relatively mild and usually does not cause severe thermal effects or other side reactions.
In terms of melting point, a specific melting point value provides an important basis for identification and purity judgment. Accurate determination of the melting point can determine whether the purity of the substance is up to standard. Generally speaking, the melting point of high-purity TES sodium salts is relatively fixed. If it contains impurities, the melting point may be offset.
In addition, the density of the substance also has its own characteristics. Although it is not a parameter that is frequently considered on a daily basis, in some experiments involving precise measurement or specific system construction, density data is indispensable, which is related to the precise configuration of the system and the control of the reaction process. In short, the various physical properties are interrelated and jointly determine the applicability and application of N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt in different scenarios.
What should I pay attention to when storing N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt?
N- (tris (hydroxymethyl) methyl) -2 -aminoethanesulfonic acid, sodium salt, this is a commonly used reagent for biochemical experiments. Be careful when storing, and keep the following points in mind:
First, the control of temperature and humidity. It should be stored in a cool and dry place away from direct sunlight. High temperature will cause denaturation of the reagent, and high humidity can make it deliquescent, which affects the quality. Due to the increase in temperature, it can accelerate the movement of molecules, promote chemical reactions, and damage the purity and activity of the reagent; if the humidity is too high, water vapor will adhere to the reagent, or cause it to dissolve or agglomerate. Generally speaking, it is better to store in a refrigerated environment of 2-8 ° C. In some specific cases, lower temperatures may be required.
Second, airtightness is essential. Be sure to ensure that the package is well sealed to prevent air and moisture from invading. The reagent may react with carbon dioxide and oxygen in the air to change its properties. If carbon dioxide interacts with it, or causes its pH to change, which in turn affects the experiment. After taking it, seal the package quickly and do not expose it to the outside for a long time.
Third, store it in categories. Do not mix with oxidants, acids, alkalis, etc. Because of its special chemical properties, coexist with other chemical substances, or react violently, causing danger. If in contact with strong oxidants, there may be a risk of combustion or explosion; improperly mix with acid and alkali, or deteriorate due to reaction.
Fourth, clear identification. On the package, the logo should be clearly marked, and the name, specification, production date, shelf life, etc. can be used for management, and the production date and shelf life can be used to judge whether the reagent can continue to be used, so as to avoid the use of expired deterioration and cause experimental deviation.
How stable is N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt in different pH environments?
N- (tris (hydroxymethyl) methyl) -2 -aminoethanesulfonic acid, sodium salt, commonly known as TES sodium salt, is a common buffer in biochemical research. Its stability under different pH environments is a matter of great concern to researchers.
In an acidic environment, if the pH is too low, this substance may protonate. Because its structure contains protonated groups such as amino groups and sulfonic acid groups. Protonation may change the charge distribution and molecular configuration of the compound, which in turn affects its buffering capacity. However, in a moderately acidic environment, the structure of TES sodium salt is still stable and can still play a buffering effect.
In an alkaline environment, when the pH is too high, some chemical bonds in the molecules may be affected. For example, hydroxyl groups may deprotonate, affecting the interaction between molecules. However, on the alkaline side of the common buffer range, TES sodium salt can still maintain relative stability and effectively regulate the pH of the system.
Generally speaking, the effective buffer range of TES sodium salt is about pH 6.8-8.2. Within this range, the substance has good stability and can effectively maintain the pH stability of the system by adjusting its own acid-base balance. However, beyond this range, with the deviation of pH, its stability gradually decreases and its buffering capacity gradually weakens. Therefore, when using TES sodium salt as a buffer, researchers need to precisely control the pH environment according to the experimental requirements to ensure the good stability of the substance, so that the buffer system can play the best performance.
What are the synthesis methods of N- (Tris (Hydroxymethyl) Methyl) -2-Aminoethanesulfonic Acid Sodium Salt?
The synthesis method of N- (tris (hydroxymethyl) methyl) -2 -aminoethanesulfonate sodium salt, often referred to as TES sodium salt, is as follows:
First take an appropriate amount of tris (hydroxymethyl) aminomethane and place it in a clean reaction vessel. Dissolve it fully with an alcohol solvent such as ethanol to form a uniform solution. This alcohol solvent not only helps to dissolve, but also stabilizes the reaction environment during the reaction process, so that the reaction can proceed smoothly.
Then, under stirring, slowly add an aqueous solution of sodium 2-chloroethanesulfonate dropwise. This dropwise process requires strict control of the rate to prevent overreaction. Sodium 2-chloroethanesulfonate is the key reactant, and its reaction with tris (hydroxymethyl) aminomethane is the core step in the synthesis.
After the dropwise addition is completed, increase the temperature of the reaction system to a suitable range, such as 50-70 ° C, and continue to stir the reaction at this temperature for a number of times. The act of heating up can speed up the molecular movement and promote the effective collision between the reactants, thereby accelerating the reaction rate and making the reaction more sufficient.
After the reaction is completed, the reaction mixture is cooled to room temperature. After that, the solvent in the system is removed by vacuum distillation. Vacuum distillation can achieve the separation of the solvent at a lower temperature to avoid the decomposition of the product due to high temperature.
After removing the solvent, the crude product is obtained. Then purified by recrystallization. Select a suitable solvent, such as ethanol-water mixed solvent, dissolve the crude product, heat it until it is completely dissolved, and then slowly cool it to make the product crystallize and precipitate. The recrystallization process can effectively remove impurities and improve the purity of the product.
Finally, the crystals are collected by filtration, and the water on the surface of the crystal is removed by low temperature drying to obtain a pure N- (tris (hydroxymethyl) methyl) -2 -aminoethanesulfonate sodium salt product.
