N-[Tris(Hydroxymethyl)Methyl]-2-Hydroxypropanesulfonic Acid, Sodium Salt

N - [Tris (Hydroxymethyl) Methyl] - 2 - Hydroxypropanesulfonic Acid, Sodium Salt, namely N - tris (hydroxymethyl) methyl - 2 - hydroxypropane sulfonate sodium salt, its main application fields are as follows:
In the field of biochemistry and molecular biology experiments, this substance is often used as a buffer. In many experimental reaction systems, it can effectively maintain a stable pH environment. Due to many biochemical reactions, it is extremely sensitive to pH value. A slight deviation may cause the reaction rate to change, or even make the reaction unable to proceed normally. This substance can quickly react with acidic or basic substances in the system when they increase by virtue of its specific acid-base balance characteristics, to ensure pH stability, and to help various biomolecules, such as proteins, nucleic acids, etc., maintain their inherent structure and activity in a suitable environment, so that relevant experiments can be carried out accurately.
In the field of cell culture, it also plays a key role. Cell growth requires a strict pH value of the environment in which it is located. Adding this substance as a buffer component can make the pH value of the cell culture medium constant in the range suitable for cell growth, creating a stable living environment for cells, which is conducive to cell proliferation, differentiation and maintenance of normal physiological functions. It has greatly promoted the research of cell biology and large-scale cell culture in biopharmaceuticals.
It is indispensable in electrophoresis experiments. The electrophoresis process requires precise control of pH conditions to achieve effective separation and analysis of biomacromolecules. As an important component of the buffer system, this substance can ensure the stability of the pH value of the buffer during the electrophoresis process, allowing proteins, nucleic acids and other macromolecules to migrate and separate ideally in the electric field according to their own charges and molecular sizes, thus providing strong support for researchers to obtain accurate experimental results.

N - [Tris (Hydroxymethyl) Methyl] - 2 - Hydroxypropanesulfonic Acid, Sodium Salt, the Chinese name is often N - tris (hydroxymethyl) methyl - 2 - hydroxypropane sulfonate monosodium salt, is a commonly used buffer in biochemical research. Its physical and chemical properties are as follows:
Look at its properties, it is a white crystalline powder at room temperature, with fine texture, easy access and operation, and this state is conducive to storage and subsequent experimental preparation.
In terms of solubility, the substance has good solubility in water, and can quickly and uniformly disperse in the aqueous system to form a uniform and stable solution. This property is very important, because as a buffer, it is often required to configure a specific concentration of aqueous solution, and good water solubility can ensure that it can fully play a buffering role in the solution.
Its buffering performance is excellent, within a specific pH range, it can effectively resist the pH change caused by the addition of a small amount of acid or base, and maintain the pH value of the solution relatively stable. This specific pH range is compatible with the suitable environment for many biochemical reactions, and is widely used in biochemical experiments and simulation of physiological processes in vivo. It can ensure that the structure and function of biological macromolecules are not affected by large fluctuations in pH.
Thermal stability is also worth mentioning. Its chemical structure and properties remain stable within a certain temperature range, and will not decompose or deteriorate due to moderate changes in temperature. This allows it to continuously play a stable buffering role in experimental operations involving temperature changes, such as some biochemical reactions that require heating or cooling steps, providing a strong guarantee for the reliability and repeatability of experimental results.

N - [Tris (Hydroxymethyl) Methyl] - 2 - Hydroxypropanesulfonic Acid, Sodium Salt, Chinese name: N - tris (hydroxymethyl) methyl - 2 - propane sulfonic acid, its storage Study.
This product should be suitable for dry weather and avoid direct exposure to light. Because light is easy to cause its chemical properties to be damaged, the shadow product is not good. Its temperature is also sensitive, and the tide can be desiccated and so on. Therefore, it is necessary to maintain the temperature of the environment. The temperature should be controlled at a certain temperature, usually as low as 60%.
In addition, the temperature is also a factor. High resolution may cause decomposition and reaction. Generally, the storage temperature is between 2 and 8 degrees Celsius, and this temperature can effectively maintain its quality. In some special combinations, if the temperature is maintained at 2-8 degrees Celsius, the protection degree does not exceed 30 degrees Celsius.
Storage, it is necessary to pay attention to the sealing performance of its package. A well-sealed package can not only block the drying of the outside world, oxygen and other materials, but also prevent it from being reacted to by the outside world or the outside world. When using the package, it can be quickly sealed as before to ensure that the remaining product is not affected. In this way, it is possible to ensure that N - [Tris (Hydroxymethyl) Methyl] - 2 - Hydroxypropanesulfonic Acid, Sodium Salt maintains good performance during storage.

To prepare N - [Tris (hydroxymethyl) methyl] -2 -hydroxypropanesulfonate sodium salt, follow the following steps:
First take an appropriate amount of Tris (trimethylol aminomethane) and place it in a clean reaction vessel. Take a certain amount of 2-hydroxypropanesulfonate with a precise measuring tool and slowly pour it into the container containing Tris. The ratio of the two needs to be precisely prepared, generally according to the stoichiometric ratio or the best ratio explored experimentally.
Then, under stirring conditions, adjust the temperature of the reaction system to a suitable range, usually between 30 ° C and 60 ° C. Continuous stirring allows the two to fully contact and react. During this process, the molecules interact and the chemical bonds are rearranged.
Wait for the reaction to proceed for a period of time, and use analytical methods such as thin-layer chromatography or high-performance liquid chromatography to monitor the reaction progress until the reaction reaches the desired level.
Then, slowly add the sodium hydroxide solution to the reaction mixture. The alkali addition process needs to be careful, pay attention to the pH change of the reaction system, so that the pH is maintained in a specific range, generally between 8 and 10, to promote the formation of the target sodium salt.
After adding the alkali, continue to stir for a period of time to ensure the complete reaction. After that, use vacuum distillation to remove the solvent in the system and concentrate the product.
The concentrated mixture is recrystallized with a suitable organic solvent. Select a solvent suitable for the solubility of the product, such as ethanol, acetone, etc. After multiple recrystallization, the purity of the product can be effectively improved.
The crystallization is completed, and the Brinell funnel is used with a suction filter device to separate the crystals from the mother liquor. The obtained crystals are washed with a small amount of cold organic solvent to remove impurities attached to the surface.
Finally, the washed crystals are placed in an oven and dried at a suitable temperature to obtain a pure N - [Tris (hydroxymethyl) methyl] -2 -hydroxypropanesulfonate sodium salt product. The whole preparation process requires attention to the proportion of raw materials, reaction temperature, pH control and purification steps to obtain the ideal product.

N - [Tris (Hydroxymethyl) Methyl] - 2 - Hydroxypropanesulfonic Acid, Sodium Salt is TAPS sodium salt, which is used in different industries at different concentrations.
In biochemistry and molecular biology experiments, it is often used as a buffer to maintain a stable pH environment. In protein electrophoresis experiments, its concentration is generally 10-50 mM. Because protein separation is extremely sensitive to pH, this concentration range can effectively control the pH in the appropriate range, ensure the stability of protein charge and mobility, and achieve good separation effect. In enzyme activity studies, 20-100 mM is usually used. Different enzymes have a specific optimum pH. The concentration of TAPS sodium salt buffer can simulate the physiological environment of enzyme action, ensure that the enzyme activity is in the best state, and facilitate the accurate study of enzyme catalytic mechanism and kinetic parameters.
In the field of cell culture, the concentration is generally used in the range of 5-25 mM. Cell growth requires strict environmental pH. This concentration of TAPS sodium salt buffer can maintain the pH stability of the culture medium, avoid adverse effects on cell growth, metabolism and morphology due to pH fluctuations, and create a suitable microenvironment for cells to survive.
In some industrial production, such as fermentation industry, the concentration may be 1-10 g/L. Microbial metabolism during fermentation can cause pH changes in the system, affecting fermentation efficiency and product quality. This concentration of TAPS sodium salt can adjust and stabilize pH, ensure the normal growth and metabolism of microorganisms, and promote the smooth progress of fermentation.