What is the chemical structure of N- [Tris (Hydroxymethyl) Methyl] -3-Amionpropanesulfonic Acid
N - [Tris (Hydroxymethyl) Methyl] - 3 - Amionpropanesulfonic Acid, Chinese name: N - Tris (Hydroxymethyl) Methyl - 3 - Aminopropanesulfonic Acid, often used for TAPS. Its chemical properties are specific.
This compound is composed of three methyl groups (- CH ² OH) in the same central carbon atom. This central carbon atom has another nitrogen atom, and the nitrogen atom has a propanesulfonic acid group (- CH ² CH ² SO 🥰 H).
Looking at the whole framework, based on the core of the central carbon atom, the methyl part can be used for multiple functions due to the existence of the methyl group, which can give the molecule a certain degree of stability and water resistance. The sulfonic acid group (-SO-H) in the propanesulfonic acid group is easy to dissolve in aqueous solution, and is acidic, so that the compound has certain characteristics.
This transformation makes TAPS useful in biochemical research, such as it is often used as a catalyst. Because of its specific acidity, it can effectively maintain the pH value of the solution and play an important role in the polybiochemical reaction system.
N- [Tris (Hydroxymethyl) Methyl] -3-Amionpropanesulfonic Acid is commonly used in which experiments
N - [Tris (Hydroxymethyl) Methyl] - 3 - Amionpropanesulfonic Acid, Chinese name tris (methyl) methylaminopropane sulfonic acid, often used as TAPS. This compound is used for multiplication in polymers.
In biochemical and molecular biology, it is often used as a catalyst. Because of its good performance, it can effectively maintain the pH value of the solution. In protein research, the protein's function is sensitive to the pH value of the environment. TAPS can determine the pH of the protein, prevent the protein from changing due to pH waves, and maintain normal activity. It is convenient for researchers to further explore the characteristics of proteins.
In enzymatic reaction, the catalytic activity of enzymes depends on a specific pH. TAPS can provide an appropriate pH environment, so that enzymes can achieve the best activity, thus assisting researchers in their research on enzymatic reaction, resistance, etc., and laying the foundation for phase-domain research.
In cell culture, the pH value requirements of cell-generated culture are strict. Adding TAPS to the culture base can maintain a pH level, create a good environment for cell generation, and ensure normal cell generation and proliferation, which is beneficial to the development of cell-biological research.
What is the pH buffer range of N- [Tris (Hydroxymethyl) Methyl] -3-Amionpropanesulfonic Acid
N- [Tris (Hydroxymethyl) Methyl] - 3 - Amionpropanesulfonic Acid, Chinese name tris (methyl) methylaminopropanesulfonic acid, often TAPS. This is commonly used in biochemical research, and its pH is important.
Generally speaking, the pH of TAPS is between 7.7 and 9.1. At this pH level, TAPS can exhibit good performance and can effectively maintain the pH value of the solution. In biochemical applications, such as protein analysis, enzyme activity determination, etc., it is necessary to control the pH environment to ensure that the function of biomolecules is not affected. Due to its specific performance, TAPS can be filled with high-quality materials under conditions of near-neutral to weak performance. This unique property makes it applicable to the research of polybiotic molecular biology, providing reliable pH conditions and ensuring the reproducibility of fruits.
N- [Tris (Hydroxymethyl) Methyl] -3-Amionpropanesulfonic Acid
N - [Tris (Hydroxymethyl) Methyl] - 3 - Amionpropanesulfonic Acid, Chinese name: N - tris (methyl) methyl - 3 - aminopropanesulfonic acid, often used for TAPS. This chemical is of paramount importance for proper storage, because its properties affect its performance in chemical and biological applications.
Preservation of TAPS is the first to promote the dry, dry and transparent environment. Because of its absorbency, the humid environment is easy to cause it to absorb water, and it can change its own temperature and chemical properties. Therefore, it should be stored in a dry machine, or use a well-sealed container to block the outside world.
The temperature should not be ignored, and it should be placed in a low-temperature environment. It is generally refrigerated, and the temperature should be 2-8 ° C. In this way, the molecular activity can be effectively reduced, and it may be reversed, such as degradation or polymerization, and its shelf life can be extended.
In addition, TAPS is sensitive to light, and light may cause some photochemical reactions, which will harm its performance. Therefore, it is necessary to use opaque containers for storage, or store it in a dark place to prevent it from being exposed to light and shadow.
Use the TAPS, and it must be used to protect the equipment and dryness used to prevent introduction. And the operation should be rapid, with less exposure to the external environment.
Therefore, in order to properly preserve N - [Tris (Hydroxymethyl) Methyl] - 3 - Amionpropanesulfonic Acid, it is necessary to pay attention to the dryness of the environment, the suitability of the temperature and the shading of the light, and keep the operation in use. Only in this way can we ensure the stability of the product and ensure the reliability of the product.
What are the advantages of N- [Tris (Hydroxymethyl) Methyl] -3-Amionpropanesulfonic Acid over other buffers
N - [Tris (Hydroxymethyl) Methyl] - 3 - Amionpropanesulfonic Acid, or TAPS, is a commonly used zwitterion buffer in biochemical research. Compared with other buffers, it has several significant advantages.
Bearing the brunt, TAPS has an ideal pH buffer range, about pH 7.7 to 9.1. This range meets the needs of many biochemical reactions and biological systems, such as specific enzymatic reactions, protein stability, etc., which can effectively maintain the pH stability of the system and create a suitable acid-base environment for related biological processes.
Furthermore, the chemical properties of TAPS are quite stable. Under common experimental conditions, it is not easily disturbed by factors such as oxidation and hydrolysis, thus ensuring the reliability and repeatability of the buffer system for a long time. Researchers do not need to change buffers frequently, and the experimental results are more reliable.
Moreover, TAPS has a weak chelation ability for most metal ions. This means that in the reaction system involving metal ions, it will not strongly interact with metal ions, which in turn affects the activity or reaction process of metal ions, which is conducive to the accurate study of biochemical reactions dependent on metal ions.
In addition, the interaction between TAPS and biomacromolecules is weak. This property allows the study of biological macromolecules such as proteins and nucleic acids without interfering with their structure and function, and can truly reflect the behavior and characteristics of biological macromolecules under physiological conditions, providing a strong guarantee for accurately exploring the mechanism of biological macromolecules.