What is the chemical structure of Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid]?
Piperazine - 1,4 - Bi [2 - Hydroxypropanesulfonic Acid] has a unique chemical structure. The piperazine ring is a six-membered heterocyclic ring composed of two nitrogen atoms and four carbon atoms alternately connected. At positions 1 and 4 of the piperazine ring, a 2-hydroxypropanesulfonic acid group is connected.
2-hydroxypropanesulfonic acid group, one end of the propyl group is connected to the nitrogen atom of the piperazine ring, while the other position of the propyl group is connected to the hydroxyl group and the sulfonic acid group. Sulfonic acid-SOH gives the substance good water solubility and ionic properties, and plays a key role in many chemical reactions and application scenarios. It can participate in the ion exchange process as an ion exchange check point. The existence of hydroxy-OH increases the hydrophilicity of the molecule on the one hand, and on the other hand enables it to participate in various organic reactions, such as esterification reactions, which expands the chemical activity and application scope of the substance. Overall, the chemical structure of Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid] combines the stability of piperazine ring, the reactivity of hydroxyl group and the ionic characteristics of sulfonic acid group, which determines its unique application value and chemical reaction performance in many fields, such as biomedicine, chemical catalysis, etc.
What are the common uses of Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid]?
Piperazine-1,4-bis [2-hydroxypropanesulfonic acid], often used as a biological buffer, has a wide range of uses in biochemical experiments.
It is indispensable in the study of biological macromolecules such as proteins and enzymes. When isolating and purifying proteins, maintain a stable pH environment to ensure that the structure and activity of proteins are not damaged. Such as chromatographic separation of proteins, at a specific pH value, proteins are separated according to their charge characteristics. This substance is used to precisely regulate pH to obtain high-purity proteins.
Enzyme activity research is also crucial. Enzymes are extremely sensitive to the pH of the reaction environment, and different enzymes have the best pH value. Like the determination of amylase activity, this buffer is used to create a suitable pH, so that the enzyme exhibits the best activity, and accurately explores the catalytic characteristics and kinetic parameters of the enzyme.
In the field of cell culture, it also plays a key role. Cell growth requires strict environmental pH, and small changes may cause abnormal cell metabolism, growth inhibition or even death. Adding this substance can stabilize the pH of the culture medium, create a stable living environment for cells, and ensure normal cell growth and proliferation. It is often used in animal cells, plant cells and microbial cell cultures.
In addition, in electrophoresis experiments, the buffer system needs to stabilize the pH to facilitate the separation of biological macromolecules according to differences in charge and molecular weight. This substance can establish a stable buffer environment, ensuring reliable and reproducible electrophoresis results, and assisting researchers in accurately analyzing the characteristics of biological macromolecules.
What are the precautions for Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid] during storage and transportation?
For piperazine-1,4-bis [2-hydroxypropanesulfonic acid], all matters need to be cautious during storage and transportation.
Although this compound is relatively stable, it still needs to be protected from heat and moisture. It should be placed in a cool, dry and well-ventilated place. If exposed to high temperature, or its properties may vary, it will affect the quality. High humidity environment may also cause deliquescence and other conditions, which will damage its purity.
When transporting, the packaging must be tight to prevent leakage. The packaging material used should be corrosion-resistant to avoid reacting with the compound. And during handling, it should be handled with care to avoid damage to the packaging.
Furthermore, the place of storage and transportation should be kept away from fire sources, oxidants and other substances. Due to its flammability, it may also react violently when exposed to oxidants, endangering safety.
In addition, the storage place should be clearly marked, indicating the name, characteristics and precautions of the compound. Management personnel should also be familiar with the properties of this substance in order to deal with emergencies. When transporting, relevant personnel also need to know the emergency response methods to ensure the safety of transportation. In this way, the quality and safety of Fangpopiperazine-1,4-bis [2-hydroxypropanesulfonic acid] during storage and transportation are comprehensive.
What are the physicochemical properties of Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid]?
Piperazine - 1,4 - Bi [2 - Hydroxypropanesulfonic Acid], the Chinese name is often piperazine - 1,4 - (2 - propanesulfonic acid), its physicochemical properties are as follows:
This substance is a multi-white crystalline powder under normal conditions. It is stable in nature and has good water solubility. It can be miscible in water in any ratio to form a uniform transparent solution. Its dissolution process is rapid, and it is weakly acidic in the water. The pH value varies according to the degree. Usually, the pH value of the dilute liquid is around 4.0-6.0.
As far as the melting boiling temperature is concerned, the melting phase is high, and it begins to melt and decompose gradually when it is added to < 300 ° C. This high melting property makes it possible to maintain a solid state in normal conditions. Its decomposition degree also shows that the biochemical properties can be changed under a certain degree of high temperature, and the quality is good.
In addition, it has a certain ability. In some chemical or biological systems, it can maintain the pH value of the phase, and resist the large pH wave caused by the addition of a small amount of acid. This property makes it important in biological chemical analysis, chemical analysis, and other fields. The isofunctional properties of the sulfonic acid group in the synthesis show that it can not only provide some nuclear substitutions, but also show certain acidic phase inverse properties, which can be used as an important component in the synthesis of polymers.
What are the synthesis methods of Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid]?
To prepare Piperazine - 1,4 - Bi [2 - Hydroxypropanesulfonic Acid], the following method is often followed.
First take an appropriate amount of piperazine and place it in a clean reaction vessel. Use an alcohol solvent such as ethanol to fully dissolve it to create a homogeneous reaction environment. In addition, take a specific reagent containing hydroxyl groups and sulfonic acid groups, such as 2-hydroxy-1-propanesulfonic acid derivatives, and slowly add it to the above piperazine solution according to the appropriate amount ratio of substances. Here, it is necessary to precisely control the ratio of the two, which is one of the keys to this product.
In the reaction system, add an appropriate amount of catalyst, such as some organic acids or organic bases, to promote the efficient progress of the reaction. Control the reaction temperature, generally maintained in a moderate range, about 50-80 degrees Celsius, not too high to cause side reactions, nor too low to slow down the reaction. During the reaction, continue to stir to make full contact with the reactants.
After several reactions, when the system is stabilized, use thin-layer chromatography or other suitable analytical means to monitor the reaction process until piperazine and 2-hydroxy-1-propanesulfonic acid derivatives are exhausted, the product is obtained.
After the crude product is separated and purified, column chromatography is preferred, and appropriate stationary and mobile phases are selected to effectively separate the product from impurities. After recrystallization, the product was further purified with a suitable solvent, such as ethanol-water mixed solvent, to obtain high-purity Piperazine-1,4-Bi [2-Hydroxypropanesulfonic Acid], and its structure and purity were confirmed by testing methods.