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What is the Chinese name of Piperazine-1,4-Bis (2-Ethanesulfonic Acid)?
Piperazine-1,4-bis (2-ethanesulfonic acid) is an important reagent in the field of biochemical research. It is often used to prepare buffer solutions to maintain a specific pH environment and ensure that biochemical reactions can be carried out stably and accurately.
According to its naming, "piperazine" indicates the six-membered heterocyclic structure of the core of the compound, which is composed of two nitrogen atoms and four carbon atoms arranged in the middle. "1,4-bis (2-ethanesulfonic acid) " clearly states that at the 1st and 4th positions of the piperazine ring, a 2-ethanesulfonic acid group is connected to each other. Among them, the 2-ethanesulfonic acid group has a strong acidic functional group of the sulfonic acid group, which makes the whole compound have good acid-base buffering ability.
In biochemical experiments, the activities and structures of many biological macromolecules such as proteins and enzymes are extremely sensitive to the pH of the environment. The buffer solution prepared by piperazine-1,4-bis (2-ethanesulfonic acid) can effectively resist the interference of a small amount of external acid and base substances, and ensure that the pH of the reaction system is in an appropriate range, so that the biological macromolecules can maintain their normal biological functions and activities. For example, in some enzymatic reactions, the precise pH conditions play a decisive role in the catalytic efficiency and specificity of the enzyme, and the piperazine-1,4-bis (2-ethanesulfonic acid) buffer system plays an indispensable role.
What are the main uses of Piperazine-1,4-Bis (2-Ethanesulfonic Acid)?
Piperazine-1,4-Bis (2-Ethanesulfonic Acid), Chinese name piperazine-1,4-diethylsulfonic acid, often referred to as PIPES. This substance has a wide range of uses and is often used as a buffer in the field of biochemical research. In the cell culture environment, cells are extremely sensitive to the pH they are located in. PIPES can create and maintain a stable pH environment, enabling cells to grow and metabolize normally under suitable acid-base conditions, just like building a stable "home" for cells.
In protein crystallization experiments, protein crystallization requires specific and precise environmental conditions. The PIPES buffer system can finely regulate the pH, promote the orderly arrangement of protein molecules and then crystallize, just like a precise "conductor", guiding proteins to complete the "dance" of crystallization.
In addition, in the study of enzyme activity, many enzymes can only show the best activity within a specific pH range. PIPES buffer can maintain this specific pH range, helping researchers to accurately explore the activity changes of enzymes in a suitable acid-base environment, just like building a suitable "stage" for enzymes to fully display their activity.
PIPES plays a key role in many biochemical experimental fields, providing indispensable conditions for the smooth progress of related research.
What are the physicochemical properties of Piperazine-1,4-Bis (2-Ethanesulfonic Acid)?
The physicochemical properties of piperazine-1,4-bis (2-ethanesulfonic acid) are particularly important. Its properties are usually white crystalline powder, which is pure and delicate in appearance, like the condensation of snow, with uniform texture.
In terms of its solubility, it can be quite dissolved in water, just like ice crystals entering the flow, quietly disappearing, forming a uniform solution. This property makes it very convenient to use in the reaction or preparation of many aqueous phase systems. Its aqueous solution is slightly acidic, similar to the water of spring stream, slightly flexible, and the pH value is often stable in a specific range. This acid-base property is crucial for maintaining the environmental stability of certain biochemical reactions.
In addition, its thermal stability should not be underestimated. In a moderate temperature range, such as a still lake, its structure and properties can remain stable. However, if the temperature is too high, it will also boil and overflow, and the structure may change. Therefore, when storing and using, it is necessary to pay attention to the control of temperature.
In addition, piperazine-1,4-bis (2-ethanesulfonic acid) has a certain chelating ability to metal ions, like a gentle arm that gently surrounds metal ions. This property can play a role in regulation and stability in some reactions or systems involving metal ions. Its chemical properties are relatively stable, and it is not easy to react easily with common weak oxidants or reducing agents. It seems to be hidden in the market, calm and restrained. However, under specific conditions, it can also exhibit unique chemical activity and participate in delicate chemical reactions, adding a different color to the research and application in many fields.
What are the precautions for storing Piperazine-1,4-Bis (2-Ethanesulfonic Acid)?
Piperazine-1,4-Bis (2-Ethanesulfonic Acid), Chinese name piperazine-1,4-bis (2-ethanesulfonic acid), often referred to as PIPES. When storing this agent, there are many precautions that need to be clarified.
First, pay attention to the temperature and humidity of storage. Due to its nature, it should be stored in a cool and dry place. If the temperature is too high, it may change its properties and damage its effectiveness; if the humidity is too high, it is easy to deliquescent and affect the quality. If it is in hot summer or humid places in the south, it should be protected with caution.
Second, pay attention to the storage environment. It should be kept away from fire sources and oxidants, because it may have certain chemical activity, be exposed to fire or oxidants, or react violently, causing danger. The storage place should also avoid mixing with other chemicals to prevent interaction.
Third, the packaging must be in good condition. When storing, it is necessary to ensure that the packaging is sealed to prevent excessive contact with the air. If the packaging is damaged, it is easy to react with the ingredients in the air and accelerate deterioration. After taking it, the packaging should be sealed in time to maintain its stability.
Fourth, the storage period cannot be ignored. Although there is a certain shelf life, the quality may decline over time. Regular inspection is required. When it is about to expire, it is more important to pay attention to its status to ensure that the effect is the same when used.
When storing Piperazine-1,4-Bis (2-Ethanesulfonic Acid), follow the above to ensure its quality and safety for experimental or production needs.
What is the preparation method of Piperazine-1,4-Bis (2-Ethanesulfonic Acid)?
Piperazine - 1,4 - Bis (2 - Ethanesulfonic Acid), that is, piperazine - 1,4 - bis (2 - ethanesulfonic acid), often abbreviated as PIPES, its preparation method is as follows:
1. ** Preparation of starting materials **:
- Piperazine and 2 - chloroethanesulfonic acid and other main raw materials are required. Piperazine is a basic cyclic organic compound, 2 - chloroethanesulfonic acid contains active halogen atoms and sulfonic acid groups, both of which play a key role in the reaction.
- Prepare suitable solvents, such as polar aprotic solvents, such as dimethyl sulfoxide (DMSO) or N, N-dimethylformamide (DMF), such solvents can effectively dissolve raw materials and promote the reaction.
2. ** Reaction process **:
- Dissolve piperazine in an appropriate amount of solvent and stir to disperse uniformly. Because piperazine is alkaline, it can be used as a base in the reaction system to participate in the reaction process.
- Slowly add 2-chloroethanesulfonic acid solution to the piperazine solution dropwise. The dropwise speed needs to be strictly controlled to prevent the reaction from being too violent. This reaction is a nucleophilic substitution reaction. The orphan of the piperazine nitrogen atom attacks the carbon atom connected to the chlorine atom of 2-chloroethanesulfonic acid. The chlorine atom leaves as a leaving group to form a C-N bond.
- The reaction usually needs to be carried out at a certain temperature, generally controlled at 50-80 ° C. Moderate heating can speed up the reaction rate and make it easier to achieve the desired effect. At the same time, the reaction process needs to be carried out under the protection of an inert gas (such as nitrogen) to prevent the raw material and product from being oxidized by oxygen in the air.
3. ** Product Separation and Purification **:
- After the reaction is completed, the reaction mixture is first cooled to room temperature. Due to the difference in the solubility of the product at different temperatures, cooling is helpful for subsequent separation.
- Add an appropriate amount of water to the cooled mixture to dilute the reaction system and promote the dissolution of some impurities.
- Use organic solvent extraction. An organic solvent that is not miscible with water, such as ether or ethyl acetate, can be selected. After multiple extractions, the product is transferred to the organic phase and separated from most of the impurities.
- Collect the organic phase and dry it with a desiccant such as anhydrous sodium sulfate to remove the moisture in it.
- After that, the organic solvent is removed by reduced pressure distillation to obtain a crude product.
- The crude product is recrystallized, and a suitable recrystallization solvent is selected, such as ethanol-water mixed solvent, to further purify the product, and finally a high-purity Piperazine-1,4-Bis (2-Ethanesulfonic Acid) can be obtained.
