What is the Chinese name of Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt?
Piperazine-1,4-bis (2-ethanesulfonic acid) disodium salt, which is the name of a chemical substance. Its full English name is Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt, which is quite commonly used in the field of biochemical research. It is often used to prepare buffer solutions because it can effectively maintain the pH stability of the solution and plays a key role in many biochemical reactions and experimental systems.
Looking at its structure, the piperazine ring is the core, and the two ends are connected to ethanesulfonic acid groups, and it is in the state of disodium salt. This structure gives it specific chemical properties and buffering properties. In solution, it can moderately release or accept protons according to the change of environmental pH, so as to achieve precise regulation of pH value.
It is widely used in protein research, enzyme activity determination and other experiments. In the process of protein crystallization, a suitable pH environment is of great significance for the correct folding and crystallization of proteins. The buffer solution prepared with this substance can create a stable environment and help protein crystallization to be successful. When enzyme activity is measured, the activity of the enzyme is extremely sensitive to pH. Using this substance to prepare a buffer system can allow the reaction to proceed under suitable pH conditions, ensuring that the enzyme activity can be fully displayed, so that the determination results are more accurate and reliable.
In addition, in the field of cell culture, cell growth requires strict environmental pH, and the buffer containing this substance can ensure the stability of the pH of the cell culture environment, create good conditions for cell growth and metabolism, and help cells survive and proliferate healthily. In short, piperazine-1,4-bis (2-ethanesulfonic acid) disodium salt plays an indispensable role in many aspects of biochemical experiments, and has made great contributions to the development of life science research.
What are the main uses of Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt?
Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt, Chinese name piperazine-1,4-bis (2-ethanesulfonic acid) disodium salt, often referred to as PIPES disodium salt. This substance is mainly used in the field of biochemistry and molecular biology experiments.
In biochemical research, it is an extremely important buffer. In many chemical reactions in organisms, it can effectively maintain the stability of the pH value of the reaction system. Enzymatic reactions in organisms are extremely sensitive to pH value, and subtle pH fluctuations may cause changes in enzyme activity or even inactivation. With its stable buffering ability, PIPES disodium salt can create a suitable pH environment to ensure the normal progress of enzymatic reactions. For example, in the process of protein separation and purification, the stability and activity of the protein depend on the pH value of the environment. PIPES disodium salt can maintain the pH of the solution within the stable range of the protein, prevent protein denaturation, and help obtain high-purity and high-activity proteins.
In the field of cell culture, PIPES disodium salt is also indispensable. When cells are cultured in vitro, the growth environment is strict, and pH value is one of the key factors. It can adjust the pH value of the cell culture medium, creating an acid-base environment similar to that in the body for cells to ensure normal cell growth and proliferation. Taking mammalian cell culture as an example, the appropriate pH range is usually between 7.2 and 7.4. PIPES disodium salt can stabilize the pH range of the culture medium and improve the success rate and quality of cell culture.
In addition, in molecular biology experiments such as DNA and RNA extraction and analysis, PIPES disodium salt is often used as a component of the buffer system. The structure and function of nucleic acids are significantly affected by pH. Using its buffering effect can avoid damage to nucleic acids due to pH changes during operation and ensure the accuracy and reliability of experimental results.
What are the storage conditions for Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt?
Piperazine-1,4-bis (2-ethanesulfonic acid) disodium salt, this is the name of a chemical substance. Its storage conditions are quite important, which is related to the stability and quality of the substance.
According to the text of "Tiangong Kaiwu", this substance should be stored in a cool, dry and well-ventilated place. A cool place can avoid high temperature baking to prevent the substance from changing due to heat, causing its properties to change and lose its original effect. A dry environment can prevent water vapor intrusion. If the moisture is too heavy, or the substance is damp, causing deliquescence, etc., it will damage its purity and characteristics. Well ventilated, it can disperse the turbid gas that may accumulate, maintain the freshness of the storage environment, and help the substance to be stored safely.
Furthermore, keep away from fire sources and oxidants. Fire sources are easy to cause fires. This substance may be flammable and dangerous in case of fire; oxidants should also be far away, because it encounters the substance or triggers a violent chemical reaction, causing danger. When storing, attention should also be paid to the sealing of the container to prevent the substance from being in too much contact with the outside air, oxidation and other reactions, which can damage its quality. In this way, the piperazine-1,4-bis (2-ethanesulfonic acid) disodium salt must be properly preserved so that it can be maintained in a good state for a long time, so that it can be used when needed.
What are the precautions for using Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt?
Piperazine - 1,4 - Bis (2 - Ethanesulfonic Acid) Disodium Salt, that is, piperazine - 1,4 - bis (2 - ethanesulfonic acid) disodium salt, in the experimental use, there are a number of urgent precautions need to be paid attention to.
The first one to bear the brunt is its dissolution. When this compound dissolves, it is necessary to pay attention to the selection of solvents. Generally speaking, water is a commonly used solvent, but factors such as water temperature and stirring rate will affect its dissolution rate and degree. It is advisable to add this salt slowly with an appropriate amount of water, and at the same time add moderate stirring to promote uniform dispersion and accelerated dissolution. In case of slow dissolution, it can be heated slightly, but the temperature should not be too high to prevent the structure of the compound from being damaged.
Furthermore, the pH value of the solution cannot be ignored. This salt is often used as a buffer, and its buffer range is specific. When used, it needs to be adjusted precisely according to the pH range required for the experiment. Because of its limited buffering capacity, if it exceeds the appropriate pH range, it may be difficult to maintain a stable pH, which will affect the experimental results. Therefore, when adjusting the pH value, you should use an accurate pH meter to slowly add acid or alkali, and measure while adding until the desired pH value is reached.
Repeat, its stability is also key. This salt should be protected from harsh environments such as high temperature, high humidity and strong light during storage and use. High temperature or cause it to decompose, high humidity can easily make it absorb moisture and agglomerate, and strong light or luminescent chemical reactions can change its chemical properties. Therefore, it is advisable to store it in a cool, dry and dark place. After taking it, seal it in time to prevent deterioration.
In addition, during the experimental operation, safety protection should not be underestimated. Although this compound may not be very toxic, direct contact with skin and eyes should be avoided. When operating, it is advisable to wear appropriate protective gloves and goggles. If you contact it accidentally, you should immediately rinse it with plenty of water and take further medical measures according to the specific situation.
In conclusion, when using Piperazine - 1,4 - Bis (2 - Ethanesulfonic Acid) Disodium Salt, all aspects of dissolution, pH adjustment, stability maintenance and safety protection must be carefully carried out in order to achieve the desired experimental effect.
What are the common reactions of Piperazine-1,4-Bis (2-Ethanesulfonic Acid) Disodium Salt with other substances?
Piperazine-1,4-bis (2-ethanesulfonic acid) disodium salt, this is a commonly used chemical reagent, which is quite common in the field of biochemical research. It is active and easy to react with many substances. Its common reactions are as follows:
First, when it encounters with acids, it can generate a neutralization reaction. Because its molecule has a sulfonic acid group, it can interact with the hydrogen ion in the acid. For example, if it meets hydrochloric acid, the sulfonic acid negative ion will combine with the hydrogen ion to form the corresponding salt and water, just like the ancient yin and yang, which transformed all things. This reaction is like a clever "material exchange", and each has its own place.
Second, it also has a wonderful interaction with metal ions. In this compound, both the nitrogen atom and the oxygen atom of the sulfonate have lone pairs of electrons, which can coordinate and complex with metal ions. Taking copper ions as an example, a stable complex can be formed. The structure and properties of this complex vary according to the type of metal ions and the reaction conditions. It seems that different materials are matched with each other to create a unique form. This reaction is like building a delicate "molecular building", and the parts are closely connected.
Third, under specific conditions, it can react with nucleophiles. The carbon atoms in the ethane sulfonic acid part of the molecule are vulnerable to attack by nucleophiles in a suitable environment. The nucleophiles will replace some groups of the sulfonic acid group, causing molecular structure changes, just like a "replacement game between molecules". This reaction makes the molecule look new and gives rise to different properties.
Fourth, when it encounters a base, it also reacts. The hydrogen of the sulfonic acid group can combine with the hydroxide ion in the base to form water and the corresponding salt. This is another manifestation of acid-base neutralization, just like the two forces check and balance each other, reaching a new balance. This process is like the harmony of yin and yang, building a new order.
The above are all common reactions of piperazine-1,4-bis (2-ethanesulfonic acid) disodium salts. In the field of chemistry and biochemical research, such reactions are of great significance and can help scholars to deeply explore the nature of matter and the law of change, and then promote the progress and development of science.