What is the chemical structure of Piperazine-1,4-Diethylsulfonic Acid Monosodium Salt Pipes-Na
Piperazine - 1,4 - Diethylsulfonic Acid Monosodium Salt is Pipes - Na, and its chemical structure is as follows: The core of this compound is a piperazine ring. The piperazine ring is a six-membered nitrogen-containing heterocyclic ring composed of two nitrogen atoms and four carbon atoms alternately connected. At the 1st and 4th positions of the piperazine ring, respectively, an ethyl sulfonate group (-CH -2, CH, SO, SO, H) is connected, but this is in the form of a monosodium salt, which means that the hydrogen atom of one of the sulfonate groups (-SO, SO, H) is replaced by a sodium ion (Na, Na), forming the -SO, Na structure. This structure makes Pipes-Na have specific acid-base buffering properties, and is often used as a buffer in biochemical research and other fields, which can effectively maintain the system within a specific pH range. Due to the combination of sulfonic acid groups and sodium ions in the structure, it can interact with hydrogen ions or hydroxide ions in the solution to adjust and stabilize the pH value.
What are the main uses of Piperazine-1,4-Diethylsulfonic Acid Monosodium Salt Pipes-Na
Pipes-Na is a commonly used reagent in biochemical research. Its main uses are widely used in many fields of biochemistry and cell biology.
In the context of cell culture, Pipes-Na often acts as a buffer. Cell growth requires a suitable acid-base environment. This reagent can effectively maintain the stability of the pH value of the culture medium, avoid pH fluctuations caused by cell metabolism, and affect the normal growth and function of cells. Just like creating a stable "habitat" for cells to multiply safely.
In protein research, Pipes-Na is also indispensable. The structure and function of proteins are highly susceptible to environmental factors, and pH is one of the keys. The use of Pipes-Na to build a stable buffer system can enable proteins to be studied in an environment close to physiological conditions, which can help to accurately explore the characteristics and behavior of proteins, just like paving a smooth path for protein research.
In the field of enzymatic reaction research, Pipes-Na also plays an important role. Enzymes are extremely sensitive to the pH of the reaction environment, and if there is a slight deviation, the enzyme activity will be affected. Pipes-Na can ensure the smooth progress of enzymatic reactions under suitable pH conditions, helping researchers to clarify the mechanism of enzyme action, just like a guiding light for enzymatic reactions.
In addition, Pipes-Na is also used in nucleic acid-related experiments. During the extraction, purification and analysis of nucleic acids, a stable pH environment helps to maintain the integrity and activity of nucleic acids, and Pipes-Na plays a role in escorting this process.
Piperazine-1,4-Diethylsulfonic Acid Monosodium Salt Pipes-Na What are the precautions during use
Piperazine - 1,4 - Diethylsulfonic Acid Monosodium Salt (Pipes - Na) is a commonly used buffer for biochemical experiments. When using, many things need to be paid attention to.
First, this agent is very hygroscopic, so when storing, it must be placed in a dry place and tightly sealed to prevent it from affecting the purity and buffering performance due to moisture absorption. If the agent has already absorbed moisture, it may cause the concentration of the buffer to be inaccurate, which may deviate the experimental results.
Second, when configuring the buffer, it is necessary to precisely control its concentration. The concentration of the buffer required for different experiments may vary. Be sure to use a precise measuring tool to carefully weigh an appropriate amount of Pipes - Na for configuration according to the specific requirements of the experiment. A slight deviation in concentration may affect the stability of the pH of the system and affect the structure and function of biomacromolecules, just as the activity of enzymes may be changed due to improper buffer concentration.
Third, the pH range of Pipes-Na buffer is usually between 6.1 and 7.5, and it needs to meet this pH range when used. If it exceeds this range, its buffering capacity will be greatly reduced, and it will not be able to effectively maintain the pH stability of the system, resulting in fluctuations in the pH of the experimental system, which will affect the experimental process and results.
Fourth, when mixed with other reagents, it is necessary to know in advance whether it will react with Pipes-Na. Some metal ions or specific organic reagents may react chemically with Pipes-Na, changing its chemical properties and causing interference to the experiment. Therefore, the relevant information should be carefully checked before mixing, or a small-scale test should be carried out in advance to ensure compatibility between the two.
Fifth, the prepared Pipes-Na buffer should not be stored for a long time, especially in high temperature or in an environment where microorganisms are prone to breed. If stored for a long time, the buffer may be contaminated by microorganisms, or chemical changes may occur, affecting its buffering performance. It is generally recommended to use it now. If it really needs to be stored, it should be placed in a low temperature environment and pay attention to the shelf life.
What are the storage conditions of Piperazine-1,4-Diethylsulfonic Acid Monosodium Salt Pipes-Na?
Piperazine - 1,4 - Diethylsulfonic Acid Monosodium Salt is Pipes - Na, which is a commonly used buffer for biochemical experiments. Its storage conditions are quite critical, as follows:
- ** Temperature conditions **: It is suitable to be stored in a low temperature environment, usually 2 - 8 ° C. This temperature range can effectively inhibit the activity of buffer molecules, reduce the rate of chemical reaction with the outside world, and thereby maintain its chemical stability. If the temperature is too high, the thermal motion of the molecules will intensify, which will easily cause the decomposition or deterioration of the buffer; if the temperature is too low, it may freeze, destroy the molecular structure, and affect the buffer performance.
- ** Humidity requirements **: It needs to be placed in a dry place to avoid high humidity environment. Due to its certain hygroscopicity, if the ambient humidity is too large, it will absorb moisture in the air, causing its purity to decrease, and may cause deliquescence, changing its chemical composition and properties, seriously affecting the accuracy of experimental results.
- ** Packaging seal **: Be sure to ensure that the packaging is tight. Due to its chemical activity, it is easy to react with gases such as oxygen and carbon dioxide when exposed to air. Sealed packaging can effectively isolate external gases, prevent changes in ingredients, and prolong its shelf life. After taking it, the packaging should be re-sealed in time to prevent external factors from interfering.
- ** Avoid light **: It should be stored away from light. Light may cause photochemical reactions, resulting in changes in the structure of the buffer, affecting the buffering effect. Therefore, it is recommended to store it in a brown bottle or in a cabinet protected from light to reduce the impact of light on it.
What is the approximate price range of Piperazine-1,4-Diethylsulfonic Acid Monosodium Salt Pipes-Na?
Piperazine - 1,4 - Diethylsulfonic Acid Monosodium Salt is PIPES - Na, and its price range varies due to many factors. In the context of "Tiangong Kaiwu", there is no direct comparable example of the price of this chemical. However, by analogy with the way the prices of various items described in "Tiangong Kaiwu" are considered, they are also affected by raw materials, production methods, supply and demand.
If the raw materials are easy to obtain and the production method is simple, coupled with sufficient supply and normal demand, the price may be inexpensive, per gram or several yuan. If the raw materials are rare, the preparation is complicated, or the market demand is urgent and the supply is scarce, the price will be high, and it can reach tens of yuan per gram or even higher.
In today's market, ordinary purity PIPES-Na, when purchased in bulk, ranges from 3 to 10 yuan per gram; high purity and special specifications, 20 to 50 yuan per gram. However, the market conditions are changing rapidly, and it is difficult to determine the exact price due to differences in logistics and taxes in various places. This price range is only a rough estimate for reference.