Piperazine-N,N-Bis (2-Ethanesulfonic Acid), 1.5 Sodium

Piperazine-N, N '-Bis (2-Ethanesulfonic Acid), 1.5 Sodium, Chinese name piperazine-N, N' -bis (2-ethanesulfonic acid) 1.5 sodium salt, often abbreviated as PIPES 1.5Na. This compound has a wide range of uses and has played an important role in the field of biochemical research.
First, it is used in the preparation of buffer solutions. In biochemical experiments such as cell culture and enzymatic reactions, pH stability is crucial. The suitable buffer range of PIPES 1.5Na is about pH 6.1-7.5, which can effectively maintain the pH stability of the system and create an excellent environment for the normal development of the structure and function of biological macromolecules. For example, when cells are cultured, cells are extremely sensitive to the pH of the environment in which they are located, and small changes may affect cell growth, metabolism and even survival. With the help of PIPES 1.5Na buffer solution, the pH of the extracellular liquid can be stabilized in a suitable range to ensure the healthy growth of cells.
Second, it plays a significant role in protein and nucleic acid research. In protein crystallization experiments, a suitable pH environment is the key to obtaining high-quality protein crystals. PIPES 1.5Na buffer solution can precisely regulate the pH of the system, helping protein molecules to aggregate and crystallize in an appropriate form, providing convenience for analyzing the three-dimensional structure of proteins. Similarly, in nucleic acid electrophoresis experiments, a stable pH environment can ensure the stable migration rate of nucleic acid molecules, so that the nucleic acid band separation effect is better, which is convenient for subsequent analysis and detection.
Third, it is also useful in some chemical reaction systems. Some pH-sensitive organic synthesis reactions need to be carried out under specific pH conditions to improve the yield and selectivity of the reaction. PIPES 1.5Na can adjust and maintain the pH of the reaction system as needed, promote the smooth progress of the reaction, and avoid the situation that the reaction by-products increase due to pH fluctuations or the reaction cannot proceed normally.

Piperazine - N, N '- Bis (2 - Ethanesulfonic Acid), 1.5 Sodium (piperazine - N, N' -bis (2 - ethanesulfonic acid) 1.5 sodium salt, referred to as PIPES 1.5 Na), this is a commonly used reagent in biochemical research. Its chemical properties are unique, let me tell you in detail.
This compound has good buffering properties and can effectively maintain the stability of the pH of the solution within a specific pH range. Its buffering range is about pH 6.1 to 7.5, acting as a loyal guardian, guarding the pH value of the solution from large fluctuations. In many biochemical and cell biology experiments, it is of great significance for maintaining a suitable microenvironment. When cells are cultured, such a stable pH environment is required to ensure the normal growth and metabolism of cells.
PIPES 1.5Na is soluble in water, which makes it very convenient to prepare various solutions. And its chemical properties are relatively stable. Under normal temperature and pressure, it does not easily react with common substances, making it easy to store and use for a long time. However, although its stability is good, when it encounters special chemicals such as strong oxidants, chemical reactions will still occur, causing changes in its structure and properties.
In addition, in the molecular structure of PIPES 1.5Na, the piperazine ring interacts with ethanesulfonic acid groups, giving it unique chemical activity and solubility. This structural property not only determines its buffering properties, but also affects its interaction with biomacromolecules. In experiments such as protein crystallization, this property can be used to assist proteins in forming an ordered crystal structure, assisting researchers in exploring the fine structure of proteins.

Piperazine-N, N '-bis (2-ethanesulfonic acid), 1.5 sodium salt (referred to as PIPES 1.5Na), has a rather subtle impact on the environment. This substance is widely used in industry and scientific research, but after it enters the environment, its impact gradually becomes apparent.
First of all, from the perspective of chemical properties, PIPES 1.5Na has good buffering properties, in water or soil, or affects local acid-base balance. Although its buffer range is limited, it affects microbial metabolism and reproduction in microscopic ecology, such as the microenvironment where specific microorganisms live, or causing acid-base upheaval.
Furthermore, bioaccumulation is also a key. If it enters the natural water body with sewage, some aquatic organisms may ingest and accumulate this substance. Although there is currently no conclusive evidence that it is highly enriched in organisms, long-term low-dose exposure may cause changes in biological physiology, such as affecting the respiratory function of gills of fish, hindering gas exchange, and affecting survival.
In addition, it also has an effect on soil ecology. PIPES 1.5Na or complexes with metal ions in the soil, altering the activity and mobility of metal ions. This process may affect the availability of soil nutrients, interfere with the absorption of nutrients by plant roots, and cause plant growth to be inhibited. For example, it may cause the morphology of trace elements such as iron and zinc to change in the soil, making it difficult for plants to ingest, showing symptoms of nutrient deficiency.
Although the research on the environmental impact of this substance has not been perfect, it has already begun to show signs. In the future, further investigation is needed to clarify its long-term and comprehensive impact on the ecosystem and provide a solid basis for environmental protection and rational use.

Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), 1.5 Sodium (referred to as PIPES sodium salt), this is a commonly used reagent for biochemical research. When storing and transporting, many key matters should be paid attention to.
The first thing to pay attention to is the storage temperature. This reagent should usually be stored in a refrigerated environment of 2-8 ° C. If the temperature is too high, or the chemical properties of the reagent are changed, its molecular structure may be affected, thereby reducing the activity of the reagent and affecting the accuracy of the experimental results. On the contrary, if the temperature is too low, such as below 2 ° C, some reagents may crystallize and precipitate, which also causes inconvenience to their use.
Humidity control cannot be ignored. The reagent should be placed in a dry place and avoided in a humid environment. Due to high humidity, the reagent is easy to absorb moisture, or cause moisture, resulting in a decrease in its purity. The reagent after deliquescence, or the introduction of impurities, interferes with the experimental process and affects the reliability of the experimental conclusions.
When transporting, ensure that the packaging is intact. This reagent is mostly bottled, and collisions and bumps should be prevented during transportation to avoid glass bottle cracking and reagent leakage. And the transportation process also needs to maintain suitable temperature conditions, try to match the storage temperature requirements, and ensure that the quality of the reagent is not affected by transportation.
In conclusion, proper storage and transportation of Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), 1.5 Sodium is of great significance to the quality of reagents and to ensure the smooth development of experiments and the accuracy of results.

To prepare Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), 1.5 Sodium (piperazine - N, N - bis (2 - ethanesulfonic acid) 1.5 sodium salt), the following methods can be used:
First, the raw materials need to be prepared. Piperazine is the key starting material, and 2 - chloroethanesulfonic acid or its suitable salts are required as sulfonic acid introduction reagents. At the same time, alkali substances such as sodium hydroxide are used to adjust the pH and promote the reaction during the reaction, and can promote the formation of sodium salts of the product.
To start the reaction, place an appropriate amount of piperazine in a suitable reaction vessel, and add an appropriate amount of solvent, such as polar organic solvents such as methanol, ethanol or a mixed system of water and organic solvents, to help the material disperse and the reaction proceed. Then, under stirring, slowly add 2-chloroethanesulfonic acid or its salts. This process should be controlled at temperature, which can be started at low temperature, and then moderately heated according to the reaction process, so that the reaction can occur smoothly. Generally speaking, the initial temperature can be set to 0-10 ° C, followed by heating to 30-50 ° C for continuous reaction.
During the reaction, with the help of a pH meter to monitor the pH of the system, a sodium hydroxide solution is added dropwise in a timely manner to maintain the reaction system in a suitable pH range, usually between 7 and 9, which is conducive to the forward progress of the reaction and the formation of products. The reaction lasts for a period of time, about 2 to 6 hours. During this period, regular sampling and monitoring are carried out. The reaction process and product formation are observed by means of thin layer chromatography (TLC) or high performance liquid chromatography (HPLC).
When the reaction reaches the desired level, that is, the reaction of the raw materials is complete or the proportion of the products reaches a satisfactory value, stop heating and stirring. Then, the reaction solution is cooled to room temperature, at which time solid may precipitate. If there is no precipitation, some of the solvent can be removed by vacuum distillation to promote the crystallization of the product. The resulting solid is collected by suction filtration and washed with an appropriate amount of cold solvent to remove impurities.
Finally, the obtained crude product is recrystallized and purified. Select a suitable solvent, such as ethanol-water mixed solvent, heat to dissolve the crude product, and filter it hot to remove insoluble impurities. The filtrate is slowly cooled to recrystallize the product and precipitate it. It is collected by suction filtration again and dried in vacuum at a suitable temperature to obtain the pure product of Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), 1.5 Sodium.