What are the chemical properties of Piperazine-N, N '-Bis (2-Hydroxypropanesulfonic Acid) Dihydrate?
Piperazine -\ (N\),\ (N '\) -bis (2-hydroxypropanesulfonic acid) dihydrate, which is a commonly used chemical reagent in the field of fine chemicals. It has unique chemical properties and is widely used in many fields.
Looking at its physical properties, it is a white crystalline powder under normal conditions, with stable properties, can maintain its own shape in air, and is not easy to volatilize. This substance is highly soluble in water, forming a uniform and transparent solution. This solubility makes it convenient to operate in solution system experiments and production.
In terms of chemical properties, it is weakly acidic, and can release protons under appropriate conditions due to the sulfonic acid group in its molecular structure. In chemical reactions, it can be used as a buffer, because it can interact with acids and bases, effectively maintaining the stability of the reaction system\ (pH\) value. In biochemical experiments, many enzymatic reactions are extremely sensitive to\ (pH\), which can ensure that the enzyme is active in a suitable\ (pH\) environment and avoid enzyme inactivation due to large fluctuations in\ (pH\), which affects the experimental results.
Furthermore, because of its hydroxyl group, it can participate in a variety of organic synthesis reactions, such as esterification with carboxyl-containing compounds, providing rich possibilities for the field of organic synthesis, which can be used to prepare compounds with specific structures and functions.
Piperazin-\ (N\),\ (N '\) -bis (2-hydroxypropanesulfonic acid) dihydrate plays a key role in biochemistry, medicine, organic synthesis and other fields due to its stable physical properties and unique chemical activity, and has become an indispensable substance in many scientific research and production processes.
Piperazine-N, N '-Bis (2-Hydroxypropanesulfonic Acid) Dihydrate is commonly used in which experiments
Piperazine-N, N '-Bis (2 - Hydroxypropanesulfonic Acid) Dihydrate, Chinese name piperazine-N, N' -bis (2-hydroxypropanesulfonic acid) dihydrate, often abbreviated as POPSO-2H 2O O, which is a commonly used zwitterion buffer in biochemical research. Due to its unique chemical properties, it has important applications in many experimental scenarios.
In protein research experiments, POPSO-2H 2O O is often used to maintain a stable pH environment. Proteins are extremely sensitive to the pH value of their environment, and slight deviations may cause damage to their structure and function. POPSO-2H2O can stabilize the pH value of the solution within a specific range, creating a suitable living environment for proteins, and enabling researchers to better explore the properties, structures and functions of proteins. For example, in protein crystallization experiments, precise pH regulation is the key to obtaining high-quality protein crystals, and POPSO-2H2O plays an indispensable role in this process.
Enzyme research experiments also frequently use POPSO-2H2O. Enzymes are used as biocatalysts, and their activity is significantly affected by pH. Different enzymes have the best activity under specific pH conditions. POPSO-2H2O can build a suitable pH environment according to the characteristics of the studied enzyme to ensure the efficient activity of the enzyme, so as to facilitate in-depth study of the catalytic mechanism and kinetic parameters of the enzyme.
In cell culture experiments, POPSO-2H2O O is also crucial. Cell growth requires a stable pH environment. POPSO-2H2O can effectively buffer the acidic or alkaline substances produced by cell metabolism, maintain the pH stability of cell culture medium, and provide a strong guarantee for the normal growth and proliferation of cells. In addition, in some nucleic acid-related experiments, such as nucleic acid extraction, purification, and amplification, the stable pH environment created by POPSO-2H2O helps to maintain the structural integrity of nucleic acids and avoid nucleic acid degradation due to pH fluctuations, thus ensuring the smooth progress of the experiment and the accuracy of the results.
What is the price range of Piperazine-N, N '-Bis (2-Hydroxypropanesulfonic Acid) Dihydrate?
Piperazine-N, N '-Bis (2 - Hydroxypropanesulfonic Acid) Dihydrate, Chinese name piperazine-N, N' -bis (2-hydroxypropanesulfonic acid) dihydrate, often referred to as POPSO-2H 2O O, is a commonly used buffer in biochemical research. Its price range varies depending on many factors, making it difficult to determine.
One is related to product purity. The higher the purity, the higher the price. Scientific research-grade high-purity products have very few impurities and can accurately meet the needs of high-end experiments. The preparation process is complicated and the cost is high, so the price is high. If the purity reaches more than 99%, the price per gram may be tens of yuan. The industrial-grade purity is slightly lower, contains some impurities, and is used in industrial production with low requirements. The price is relatively low, per gram or several yuan.
Second, the manufacturers are different. Well-known large factories have mature production processes, strict quality control, stable and reliable product quality, large brand influence, and often higher prices. Small factories are competitive markets, and prices may be favorable, but product quality is uneven.
Third, the purchase volume has a great impact. If the purchase volume is small, if you only buy a few grams, the unit price will be high. If you buy a large amount, the manufacturer may give price concessions due to economies of scale, reduced sales costs, etc. When the quantity is large, the price per gram may be reduced by several yuan.
Fourth, market supply and demand determine the price. In peak demand season, supply is tight and prices rise; in off-season or oversupply, prices have room to fall.
Generally speaking, laboratories purchase scientific research-grade products in small quantities, and the price per gram may be 20-50 yuan; for large industrial purchases, the price per kilogram may be in the thousands of yuan, discounted by several yuan per gram. For specific prices, you need to inquire with suppliers and compare the quotations of different manufacturers, different purity and purchase quantity to obtain a suitable price.
How to store Piperazine-N, N '-Bis (2-Hydroxypropanesulfonic Acid) Dihydrate
Piperazine-N, N '-Bis (2 - Hydroxypropanesulfonic Acid) Dihydrate, Chinese name piperazine-N, N' -bis (2-hydroxypropanesulfonic acid) dihydrate, often referred to as POPSO-2H 2O O, is a commonly used amphoteric ion buffer in biochemical research. Its storage method is crucial, related to its quality and utility, as follows:
First, temperature control. This compound should be stored at a low temperature, and the optimal temperature range is 2-8 ° C. Low temperature environment can effectively slow down its chemical change rate, inhibit decomposition and deterioration caused by excessive temperature, and maintain its chemical stability. If placed in a high temperature environment, the thermal movement of molecules intensifies, which may cause chemical bonds to break and structure to change, which in turn affects its buffering properties.
Second, the prevention of humidity. Because of its certain hygroscopicity, it is necessary to ensure that the storage environment is dry. If the humidity is too high, it is easy to absorb moisture in the air, which not only changes its water content and affects its purity, but also may cause deliquescence, causing it to agglomerate and deteriorate. When storing, a well-sealed container should be selected, or a desiccant should be placed at the storage place to reduce the ambient humidity.
Third, it should be stored away from light. Light, especially direct light, may cause photochemical reactions, causing changes in its structure and properties. Therefore, it should be placed in a brown bottle or an opaque container and stored in a dark place to avoid adverse effects of light.
Fourth, isolate the air. Oxygen, carbon dioxide and other components in the air, or react with the compound. Sealed storage can reduce its contact with the air, prevent oxidation, carbonation and other reactions, and ensure the stability of its chemical properties.
When storing Piperazine - N, N '- Bis (2 - Hydroxypropanesulfonic Acid) Dihydrate, be sure to adhere to the principles of low temperature, dryness, protection from light, and sealing, so as to ensure its quality and make it play its due role in biochemical research and other applications.
What are the alternatives to Piperazine-N, N '-Bis (2-Hydroxypropanesulfonic Acid) Dihydrate?
Piperazine-N, N '-Bis (2 - Hydroxypropanesulfonic Acid) Dihydrate, which is piperazine-N, N' -bis (2-hydroxypropanesulfonic acid) dihydrate, often abbreviated as POPSO-2H2O O, is a biological buffer. Although this specific chemical is not present in the antiquities mentioned in Tiangong Kaiwu, there are many alternative products available today.
First, 2 - (N - morpholino) ethanesulfonic acid (MES), which is similar to POPSO-2H2O O, is a biological buffer. The pKa value of MES is about 6.15, and it can effectively maintain the pH of the solution in the pH range of 5.5-6.7. It is suitable for a variety of biochemical and molecular biology experiments, such as some enzymatic reactions. Its buffering performance can stabilize the reaction environment and ensure the activity and function of the enzyme, just like POPSO-2H2O O maintains a stable pH in a specific biological system, helping the smooth progress of related reactions.
Second, 4-morpholine ethanesulfonic acid (MOES), also a commonly used biological buffer. Its pKa is about 6.5, and the pH buffer range is 6.0-7.2. MOES is chemically stable and can avoid interfering with reactions in biochemical experiments. For example, during protein purification, it can prevent protein denaturation due to pH fluctuations, just like POPSO-2H2O O maintains the stability of the system, so that the protein retains its inherent structure and activity.
Its third, tris (hydroxymethyl) aminomethane (Tris), although the pKa value is about 8.1, the pH range is 7.0-9.0, which is different from POPSO-2H2O O, but it is widely used in the field of biochemistry. Tris can be formulated with a variety of buffers for electrophoresis, immunoblotting and other experiments. In the electrophoresis buffer, Tris can adjust the pH to ensure the stable migration of proteins or nucleic acids in the electric field, and POPSO-2H2O is also responsible for maintaining a stable environment in a specific experimental system, both of which are the keys to ensure the smooth development of the experiment.