Pipes, Monosodium Salt Piperazine-N, N-Bis (2-Ethanesulfonic Acid), What are the main uses of Monosodium Salt?
Guanshi, piperazine-N, N-bis (2-ethanesulfonic acid) monosodium salts are very widely used. In the field of biochemistry, this is a commonly used buffer. Biochemical experiments involve the control of acid-base balance, which can stabilize the pH of the system and maintain the stability of the reaction environment, so that all biochemical reactions can be carried out in an orderly manner.
Such as protein analysis and enzyme activity research, all rely on this agent. Protein has activity and structural stability in a specific acid-base environment. This agent can create a suitable acid-base atmosphere, prevent protein denaturation, and accurately analyze. When the enzyme is active, the catalytic effect of the enzyme is closely related to the environmental acid and base. Using this agent to control acid and base can probe the activity change of the enzyme under different conditions and explain the mechanism of action of the enzyme.
In the context of cell culture, it is also indispensable. Cell growth requires harsh environmental requirements, and suitable pH is essential for its growth and proliferation. This agent can stabilize the acid and base of the culture solution, create a good habitat for cells, promote healthy cell growth, and avoid cell damage or death caused by acid-base fluctuations.
In the process of drug development, drug synthesis, preparation and other links, it is necessary to control the acid and base of the reaction system. This agent can ensure the smooth reaction and improve the purity and yield of the drug. And when the drug stability is studied, the different physiological acid and base environments are used to explore the stability of the drug, and to provide evidence for the design and storage conditions of the drug dosage form.
Pipes, Monosodium Salt Piperazine-N, N-Bis (2-Ethanesulfonic Acid), what are the chemical properties of Monosodium Salt
This is the monosodium salt of Piperazine-N, N-bis (2-ethanesulfonic acid), and its chemical properties are particularly important. This compound is in the state of white crystalline powder, which has good solubility in water and can form a uniform solution.
In an acidic environment, the sulfonic acid group of this salt can accept protons and exhibit a certain acid-base buffering ability. The nitrogen atom in its structure gives it the potential to complex with metal ions. In some chemical reaction systems, it can act as a ligand and combine with metal ions to form a stable complex.
And this compound has good thermal stability. Under normal heating conditions, it is not easy to decompose and can maintain its chemical structure stability over a wide temperature range. In the field of organic synthesis, due to its unique chemical properties, it is often used as an intermediate to participate in many organic reactions and assist in the synthesis of more complex organic compounds. In biochemical research, due to its good water solubility and buffering properties, it is also often used to prepare buffer solutions to maintain the stability of acid and base in the reaction system, providing a suitable environment for the study of biomolecules.
Pipes, Monosodium Salt Piperazine-N, N-Bis (2-Ethanesulfonic Acid), What are the storage conditions for Monosodium Salt?
Piperazine - N, N - bis (2 - ethanesulfonic acid) monosodium salt, this substance should be stored in a cool, dry and well-ventilated place. Do not be near fires or heat sources to prevent its properties from changing. Because of its active chemical properties, it is easy to change when heated or wet, so the warehouse temperature should be controlled between 15 ° C and 25 ° C, and the relative humidity should not exceed 65%.
When stored, it should be separated from oxidants, acids, bases, etc., and should not be mixed. Because of its contact with these substances, or violent reaction, it is dangerous. Be sure to pack lightly when handling to prevent package damage. If the packaging is damaged, the substance is exposed to the outside, or interacts with air, moisture, etc., which will lose its effectiveness and or cause harm.
The packaging should be tightly sealed and suitable packaging materials should be selected. Usually packed in plastic outer woven bags, double-layer plastic bags or full paper drums to ensure that the substance is not damp or deteriorated during storage and transportation. The storage place should be equipped with emergency leak treatment equipment and suitable containment materials. If there is a leak, it can be dealt with quickly to reduce the harm. In this way, the quality and safety of Piperazine-N, N-bis (2-ethanesulfonic acid) monosodium salts can be guaranteed by storing in these conditions.
Pipes, Monosodium Salt Piperazine-N, N-Bis (2-Ethanesulfonic Acid), What is the production method of Monosodium Salt?
Piperazine - N, N - bis (2 - ethanesulfonic acid) monosodium salt, the method of preparation of this substance is very detailed, let me explain in detail.
First, piperazine is used as the initial raw material. First, take an appropriate amount of piperazine and place it in a clean reactor. The kettle needs to be strictly cleaned and dried to avoid impurities from disturbing the reaction. Then, slowly inject a certain amount of 2 - chloroethanesulfonic acid. In this process, it is necessary to pay close attention to the ratio of the two. Generally speaking, the molar ratio of piperazine to 2 - chloroethanesulfonic acid should be precisely regulated within a certain range to ensure that the reaction proceeds smoothly and the purity and yield of the product can be guaranteed.
Second, the reaction conditions are of the essence. The reaction temperature needs to be gradually increased. Initially, it is stirred at a moderate low temperature for a period of time to make piperazine and 2-chloroethane sulfonic acid fully mixed and initially react. Then, it is gradually warmed to a higher temperature, and the reaction is maintained at this temperature for several hours. During this period, stirring is uninterrupted to promote full contact of the reactants, so that the reaction occurs uniformly.
Third, when the reaction is approaching completion, the properties of the reaction solution must change significantly. At this time, the reaction solution needs to be cooled to an appropriate temperature, and then an appropriate amount of sodium hydroxide solution is added dropwise. This step is designed to neutralize the excess acid and promote the precipitation of the product in the form of a monosodium salt. The dropwise addition process must be slow and uniform, and the pH value of the solution must be closely monitored. When the pH value reaches a specific value, the dropwise addition is stopped.
Fourth, the precipitated product needs to be separated and purified. First, the solid product is separated from the reaction mixture by filtration. The obtained solid still contains some impurities, so it needs to be recrystallized with an appropriate solvent. Select a suitable solvent, dissolve the solid, heat it to a certain temperature, and then cool slowly to allow the product to crystallize and precipitate. After multiple recrystallization, the purity of the product can reach a high level.
Finally, the purified product is placed in a vacuum drying oven and dried at a suitable temperature for several hours to remove the residual solvent and moisture to obtain the final product of Piperazine-N, N-bis (2-ethanesulfonic acid) monosodium salt.
Pipes, Monosodium Salt Piperazine-N, N-Bis (2-Ethanesulfonic Acid), what are the application cases of Monosodium Salt in different fields
Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), Monosodium Salt (Piperazine - N, N - Bis (2 - ethanesulfonic acid) monosodium salt) are wonderful in various fields, and consider the following use cases:
In the field of biochemical research, this is an excellent buffer. Just like the ancient rock of stability, it can make the pH of the solution as stable as a rock. For example, the research and experiment of protein and nucleic acid requires precise regulation of the pH of the environment. Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), Monosodium Salt can play a buffering role in a specific pH range to ensure the safety and stability of the experimental system, so that the structure and activity of protein and nucleic acid are not disturbed by pH, just like supporting a stable world.
The environment of cell culture is also indispensable. Cell growth is like a delicate flower blooming, and it needs a delicate environment to nourish it. This salt can maintain the pH stability of the culture medium, just like fertile soil, so that cells can thrive in a suitable acid-base environment, not to decay and apoptosis due to large fluctuations in pH, to ensure the smooth cultivation of cells, and to provide a solid foundation for biomedical research, drug development, etc.
Furthermore, in molecular biology experiments, such as PCR amplification, enzyme digestion reactions, etc., it is necessary to be suitable for pH environment activation or stabilization of enzyme activity. Piperazine - N, N - Bis (2 - Ethanesulfonic Acid), Monosodium Salt is like a wise partner, which can skillfully regulate the pH of the reaction system, enable enzymes to exert the best efficiency, ensure the accuracy of experimental reactions, and achieve remarkable results. It helps researchers to explore the secrets of the microscopic molecular world.