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What are the main uses of Sodium 3-Morpholinopropanesulfonate?
Sodium 3-Morpholinopropanesulfonate, Chinese name 3-morpholinepropanesulfonate sodium, is often abbreviated as MOPSO-Na. This substance has a wide range of uses.
One is that it is often used as a buffer in biochemistry and molecular biology experiments. Many biochemical reactions in organisms are extremely sensitive to pH value, and MOPSO-Na can effectively maintain the pH stability of the reaction system, ensuring the smooth progress of various enzymatic reactions, proteins and nucleic acid related experiments. For example, in the separation and purification of proteins, different proteins have different characteristics such as charge state and solubility under a specific pH environment. The stable pH environment created by MOPSO-Na helps to achieve efficient separation of proteins according to these characteristics.
Second, in the field of cell culture, it also has important applications. Cell growth requires a strict pH value of the environment, and MOPSO-Na can keep the pH of the cell culture medium in a range suitable for cell growth, creating a good living environment for cells, and promoting normal cell growth, proliferation and metabolic activities. Like the culture of some animal cells, precise control of pH value is of great significance for cell adherent growth and maintenance of normal morphology and function, and MOPSO-Na plays an indispensable role in it.
Third, MOPSO-Na also plays a key role in the preparation of electrophoresis buffer. During electrophoresis, a stable pH environment has a profound impact on the migration rate and separation of biomacromolecules. As an electrophoresis buffer, MOPSO-Na can ensure the pH stability of the electrophoresis system, and enable the precise separation of biological macromolecules such as proteins and nucleic acids in the gel medium according to their own charge and molecular weight, which is convenient for subsequent analysis and identification.
What are the physical properties of Sodium 3-Morpholinopropanesulfonate?
Sodium 3-Morpholinopropanesulfonate, or sodium 3-morpholine propane sulfonate, is a commonly used reagent in biochemical research. Its physical properties are as follows:
This substance is usually in the form of a white crystalline powder, which is pure and delicate in appearance. Its properties are stable and easy to store and use. In terms of solubility, it is easily soluble in water and can quickly melt with water to form a uniform solution. This property makes it easy to disperse and play a role in many experiments and reaction systems. The melting point of
is critical, about 274-278 ° C. Within this temperature range, the substance converts from a solid state to a liquid state. This melting point characteristic helps to control and operate it under specific experimental conditions.
In addition, its hygroscopicity is weak, and it can better maintain its own physical state under normal ambient humidity, reducing the property changes caused by hygroscopicity, which provides a guarantee for the accuracy and stability of the experiment. In different biochemical experimental scenarios, it is these physical properties that determine that it can adapt to a variety of buffer systems, and is widely used in the research and analysis of biological macromolecules such as proteins and nucleic acids.
Is Sodium 3-Morpholinopropanesulfonate chemically stable?
Sodium 3-Morpholinopropanesulfonate, that is, sodium 3-morpholine propanesulfonate, is chemically stable. It has zwitterionic properties and can be used as a buffer in a wide pH range (about 6.5 - 7.9).
From a structural perspective, the morpholine ring is connected to the propanesulfonate group, giving the substance unique stability. The morpholine ring structure is relatively stable, which can help it resist external factors. Propanesulfonate group has strong hydrophilicity, which can enhance the solubility and stability of the substance in aqueous solution.
In terms of stability factors, it usually has certain tolerance to temperature, humidity and common chemical reagents. Under normal storage temperatures (e.g. 2-8 ° C) and normal humidity environments, it can maintain its own structure and properties stable, and will not easily decompose or deteriorate. However, when exposed to strong oxidizing agents or reducing agents, chemical reactions may occur to change its properties. At the same time, high temperature environments may also affect its stability. Excessive temperature may cause structural changes and buffer properties to decline.
In many biochemical experiments and industrial production scenarios, due to its good stability, the substance is often used to stabilize the pH value of the reaction system and ensure the smooth progress of the experiment or production process.
What are the precautions for using Sodium 3-Morpholinopropanesulfonate?
Sodium + 3 - Morpholinopropanesulfonate, that is, 3 - morpholine propane sulfonic acid, sodium salt, there are many things to pay attention to when this substance is used in experiments.
First of all, its properties need to be observed. This is a white crystalline powder. When storing, it should be placed in a cool, dry and well-ventilated place, away from heat sources and fires. Because of its hygroscopicity, if stored improperly, it is prone to moisture and affects the quality.
Furthermore, when using, its solubility must be paid attention to. Easily soluble in water, the dissolution rate may be affected by factors such as water temperature. In order to make the dissolution sufficient and fast, it can be stirred moderately or heated slightly, but the heating temperature should not be too high to prevent it from decomposing.
In addition, 3-morpholine propane sulfonic acid, sodium salt is often used in biological buffer systems, and its buffer range is very critical. The effective buffer pH range of this substance is about 6.5-7.9. In related experiments, it is necessary to ensure that the pH value of the system is within this range, so that it can exert its good buffering efficiency and maintain the stability of the pH of the system to ensure the accuracy of the experimental results.
And because it involves biological system experiments, its purity is extremely important. The presence of impurities or interference with the experimental reaction can cause deviations in the results. Therefore, the purity index should be carefully checked before use to ensure that it meets the experimental requirements.
Finally, during operation, personal protection should be paid attention to. Although it is less toxic, direct contact with skin and eyes should still be avoided. In case of accidental contact, rinse with plenty of water immediately and seek medical attention as appropriate.
What is the market price range for Sodium 3-Morpholinopropanesulfonate?
Sodium 3-Morpholinopropanesulfonate, that is, sodium 3-morpholine propane sulfonate, often abbreviated as MOPSO, is a commonly used buffer in biochemical research. Its market price range varies greatly due to a variety of factors.
Let's talk about the purity first. If it is high purity, such as for high-end scientific research and pharmaceutical research and development, it requires very few impurities, and the purity is above 99%. Such high-quality products, the price per gram may be in the tens of yuan. Due to the complex purification process required for the preparation process, the cost is high.
Depending on the packaging specifications, small packages such as 1 gram, 5 grams, for a small amount of laboratory trials, the price is relatively high, 20 or 30 yuan per gram. If it is a kilogram-level large package with a large demand such as industrial production, the unit price will be significantly reduced due to the scale effect, per gram or several yuan.
Furthermore, the difference between the manufacturer and the brand also affects the price. Well-known manufacturers pay attention to quality control, product quality is stable and reliable, and the price may be high. While some small factory products can meet the basic needs, the quality stability is slightly inferior, and the price is relatively close to the people.
Overall, Sodium 3-Morpholinopropanesulfonate market price, small package, high purity, 20 to 50 yuan per gram; large package, ordinary purity products, 1 to 5 yuan per gram. However, the market price changes dynamically, it is advisable to consult different suppliers when purchasing to get the right price.
